BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= cesb0065 (403 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1685.02c |rps1202|rps12-2|40S ribosomal protein S12|Schizosa... 151 5e-38 SPCC962.04 |rps1201|rps12-1, rps12|40S ribosomal protein S12|Sch... 149 2e-37 SPBC29A3.04 |rpl8||60S ribosomal protein L7a |Schizosaccharomyce... 31 0.067 SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyce... 29 0.20 SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces p... 27 1.1 SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|ch... 25 4.4 SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccha... 25 4.4 SPBC2G2.08 |ade9||C-1-tetrahydrofolatesynthase/methylenetetrahyd... 25 5.8 SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 24 7.7 >SPBC1685.02c |rps1202|rps12-2|40S ribosomal protein S12|Schizosaccharomyces pombe|chr 2|||Manual Length = 148 Score = 151 bits (365), Expect = 5e-38 Identities = 68/105 (64%), Positives = 83/105 (79%) Frame = +1 Query: 1 EVLKTALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQIPLVKV 180 EVLK AL+H GL G+ EA+KALD+RQA LCVL E+CD+ AY KLV+ALC E Q PLVKV Sbjct: 38 EVLKRALVHDGLARGIREASKALDRRQAHLCVLCESCDQEAYVKLVEALCAESQTPLVKV 97 Query: 181 DNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEETPALDVL 315 + K LGEWAGLC +D+DG ARK+VGCSCV + D+GE++ AL L Sbjct: 98 ADPKILGEWAGLCVLDRDGNARKVVGCSCVAVTDYGEDSVALQTL 142 >SPCC962.04 |rps1201|rps12-1, rps12|40S ribosomal protein S12|Schizosaccharomyces pombe|chr 3|||Manual Length = 145 Score = 149 bits (361), Expect = 2e-37 Identities = 66/105 (62%), Positives = 83/105 (79%) Frame = +1 Query: 1 EVLKTALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQIPLVKV 180 EVLK AL+H GL G+ EA+KALD+RQA LCVL E+CD+ AY KLV+ALC E + PL+KV Sbjct: 35 EVLKRALVHDGLARGIREASKALDRRQAHLCVLCESCDQEAYVKLVEALCAESETPLIKV 94 Query: 181 DNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEETPALDVL 315 + K LGEWAGLC +D+DG ARK+VGCSCV + D+GE++ AL L Sbjct: 95 ADPKVLGEWAGLCVLDRDGNARKVVGCSCVAVTDYGEDSAALQKL 139 >SPBC29A3.04 |rpl8||60S ribosomal protein L7a |Schizosaccharomyces pombe|chr 2|||Manual Length = 259 Score = 31.1 bits (67), Expect = 0.067 Identities = 14/54 (25%), Positives = 27/54 (50%) Frame = +1 Query: 40 HGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQIPLVKVDNNKKLG 201 +GL+ ++ ++A L ++A + D + ALC + +P V N +LG Sbjct: 134 YGLNHVVALIEAKKAKLVLIASDVDPIELVVFLPALCKKMGVPYAIVKNKARLG 187 >SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 154 Score = 29.5 bits (63), Expect = 0.20 Identities = 16/68 (23%), Positives = 32/68 (47%) Frame = +1 Query: 1 EVLKTALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQIPLVKV 180 + +K A ++ G+ E KA+ K + L +LA + + LC ++ +P + Sbjct: 43 KTVKKASKQKHILRGVKEVVKAVRKGEKGLVILAGDISPMDVISHIPVLCEDNNVPYLYT 102 Query: 181 DNNKKLGE 204 + + LGE Sbjct: 103 VSKELLGE 110 >SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 404 Score = 27.1 bits (57), Expect = 1.1 Identities = 10/25 (40%), Positives = 14/25 (56%) Frame = -2 Query: 303 RWSFLAEIFDDNTGAADNFPCLAIL 229 RW +LA +F N A N P + +L Sbjct: 257 RWEYLASLFTSNFTAVHNIPSVPLL 281 >SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|chr 3|||Manual Length = 923 Score = 25.0 bits (52), Expect = 4.4 Identities = 10/35 (28%), Positives = 19/35 (54%) Frame = +2 Query: 98 WLRTVMKPHTRNLFRLCATNIRFHWSRLTTTKSLE 202 ++ +V P L+RLC++ R W ++SL+ Sbjct: 220 FILSVFHPDAARLYRLCSSLKRICWELYDPSRSLD 254 >SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccharomyces pombe|chr 1|||Manual Length = 549 Score = 25.0 bits (52), Expect = 4.4 Identities = 10/28 (35%), Positives = 20/28 (71%), Gaps = 1/28 (3%) Frame = -1 Query: 271 QHRSSRQFSLPCHPCQSCRD-QPILQAF 191 + ++ +QF LP PCQ+ + +PI+++F Sbjct: 226 KRKALKQFPLPPDPCQALANLRPIVKSF 253 >SPBC2G2.08 |ade9||C-1- tetrahydrofolatesynthase/methylenetetrahydrofolatedehydr ogenase/methylenetetrahydrofolatecyclohydrolase/formylte trahydrofolatesynthetase|Schizosaccharomyces pombe|chr 2|||Manual Length = 969 Score = 24.6 bits (51), Expect = 5.8 Identities = 11/20 (55%), Positives = 15/20 (75%) Frame = +2 Query: 245 GKLSAAPVLSSKISARKLQR 304 G S+A +L+ K+ ARKLQR Sbjct: 28 GTSSSANILNGKLLARKLQR 47 >SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1647 Score = 24.2 bits (50), Expect = 7.7 Identities = 13/34 (38%), Positives = 17/34 (50%), Gaps = 1/34 (2%) Frame = +1 Query: 19 LIHGGLVHGLHEAAKALDKRQAVLCVLAEN-CDE 117 L+H GL+H L + K +LA N CDE Sbjct: 896 LLHSGLIHNLLLSLKKFGSSSLRTFLLAMNTCDE 929 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,514,023 Number of Sequences: 5004 Number of extensions: 28201 Number of successful extensions: 101 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 100 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 101 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 136158338 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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