BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= cesb0065
(403 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1685.02c |rps1202|rps12-2|40S ribosomal protein S12|Schizosa... 151 5e-38
SPCC962.04 |rps1201|rps12-1, rps12|40S ribosomal protein S12|Sch... 149 2e-37
SPBC29A3.04 |rpl8||60S ribosomal protein L7a |Schizosaccharomyce... 31 0.067
SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyce... 29 0.20
SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces p... 27 1.1
SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|ch... 25 4.4
SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccha... 25 4.4
SPBC2G2.08 |ade9||C-1-tetrahydrofolatesynthase/methylenetetrahyd... 25 5.8
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 24 7.7
>SPBC1685.02c |rps1202|rps12-2|40S ribosomal protein
S12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 148
Score = 151 bits (365), Expect = 5e-38
Identities = 68/105 (64%), Positives = 83/105 (79%)
Frame = +1
Query: 1 EVLKTALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQIPLVKV 180
EVLK AL+H GL G+ EA+KALD+RQA LCVL E+CD+ AY KLV+ALC E Q PLVKV
Sbjct: 38 EVLKRALVHDGLARGIREASKALDRRQAHLCVLCESCDQEAYVKLVEALCAESQTPLVKV 97
Query: 181 DNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEETPALDVL 315
+ K LGEWAGLC +D+DG ARK+VGCSCV + D+GE++ AL L
Sbjct: 98 ADPKILGEWAGLCVLDRDGNARKVVGCSCVAVTDYGEDSVALQTL 142
>SPCC962.04 |rps1201|rps12-1, rps12|40S ribosomal protein
S12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 145
Score = 149 bits (361), Expect = 2e-37
Identities = 66/105 (62%), Positives = 83/105 (79%)
Frame = +1
Query: 1 EVLKTALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQIPLVKV 180
EVLK AL+H GL G+ EA+KALD+RQA LCVL E+CD+ AY KLV+ALC E + PL+KV
Sbjct: 35 EVLKRALVHDGLARGIREASKALDRRQAHLCVLCESCDQEAYVKLVEALCAESETPLIKV 94
Query: 181 DNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEETPALDVL 315
+ K LGEWAGLC +D+DG ARK+VGCSCV + D+GE++ AL L
Sbjct: 95 ADPKVLGEWAGLCVLDRDGNARKVVGCSCVAVTDYGEDSAALQKL 139
>SPBC29A3.04 |rpl8||60S ribosomal protein L7a |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 259
Score = 31.1 bits (67), Expect = 0.067
Identities = 14/54 (25%), Positives = 27/54 (50%)
Frame = +1
Query: 40 HGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQIPLVKVDNNKKLG 201
+GL+ ++ ++A L ++A + D + ALC + +P V N +LG
Sbjct: 134 YGLNHVVALIEAKKAKLVLIASDVDPIELVVFLPALCKKMGVPYAIVKNKARLG 187
>SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 154
Score = 29.5 bits (63), Expect = 0.20
Identities = 16/68 (23%), Positives = 32/68 (47%)
Frame = +1
Query: 1 EVLKTALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQIPLVKV 180
+ +K A ++ G+ E KA+ K + L +LA + + LC ++ +P +
Sbjct: 43 KTVKKASKQKHILRGVKEVVKAVRKGEKGLVILAGDISPMDVISHIPVLCEDNNVPYLYT 102
Query: 181 DNNKKLGE 204
+ + LGE
Sbjct: 103 VSKELLGE 110
>SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 404
Score = 27.1 bits (57), Expect = 1.1
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = -2
Query: 303 RWSFLAEIFDDNTGAADNFPCLAIL 229
RW +LA +F N A N P + +L
Sbjct: 257 RWEYLASLFTSNFTAVHNIPSVPLL 281
>SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|chr
3|||Manual
Length = 923
Score = 25.0 bits (52), Expect = 4.4
Identities = 10/35 (28%), Positives = 19/35 (54%)
Frame = +2
Query: 98 WLRTVMKPHTRNLFRLCATNIRFHWSRLTTTKSLE 202
++ +V P L+RLC++ R W ++SL+
Sbjct: 220 FILSVFHPDAARLYRLCSSLKRICWELYDPSRSLD 254
>SPAPJ696.01c |vps17||retromer complex subunit
Vps17|Schizosaccharomyces pombe|chr 1|||Manual
Length = 549
Score = 25.0 bits (52), Expect = 4.4
Identities = 10/28 (35%), Positives = 20/28 (71%), Gaps = 1/28 (3%)
Frame = -1
Query: 271 QHRSSRQFSLPCHPCQSCRD-QPILQAF 191
+ ++ +QF LP PCQ+ + +PI+++F
Sbjct: 226 KRKALKQFPLPPDPCQALANLRPIVKSF 253
>SPBC2G2.08 |ade9||C-1-
tetrahydrofolatesynthase/methylenetetrahydrofolatedehydr
ogenase/methylenetetrahydrofolatecyclohydrolase/formylte
trahydrofolatesynthetase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 969
Score = 24.6 bits (51), Expect = 5.8
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = +2
Query: 245 GKLSAAPVLSSKISARKLQR 304
G S+A +L+ K+ ARKLQR
Sbjct: 28 GTSSSANILNGKLLARKLQR 47
>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
E3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1647
Score = 24.2 bits (50), Expect = 7.7
Identities = 13/34 (38%), Positives = 17/34 (50%), Gaps = 1/34 (2%)
Frame = +1
Query: 19 LIHGGLVHGLHEAAKALDKRQAVLCVLAEN-CDE 117
L+H GL+H L + K +LA N CDE
Sbjct: 896 LLHSGLIHNLLLSLKKFGSSSLRTFLLAMNTCDE 929
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,514,023
Number of Sequences: 5004
Number of extensions: 28201
Number of successful extensions: 101
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 100
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 101
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 136158338
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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