BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= cesb0035 (754 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g18600.1 68417.m02755 expressed protein 30 1.4 At2g39630.1 68415.m04858 glycosyl transferase family 2 protein s... 29 3.3 At5g15630.1 68418.m01829 phytochelatin synthetase family protein... 29 4.4 At5g49110.1 68418.m06079 expressed protein ; expression support... 28 5.8 At5g23050.1 68418.m02695 acyl-activating enzyme 17 (AAE17) nearl... 28 5.8 >At4g18600.1 68417.m02755 expressed protein Length = 1907 Score = 30.3 bits (65), Expect = 1.4 Identities = 19/55 (34%), Positives = 31/55 (56%), Gaps = 2/55 (3%) Frame = +1 Query: 124 LSIPTAV--DEMEKKELRTSATETGLNSKIGIRGTDRLEILSNVAVFDESLSDTG 282 LS+ AV +++ E +S TET ++K R T ++ S AVFDE ++ +G Sbjct: 1376 LSLKEAVFDEKIPGSEASSSTTETSPHNKTFPRETITTDLSSTEAVFDEKITGSG 1430 Score = 28.7 bits (61), Expect = 4.4 Identities = 23/84 (27%), Positives = 38/84 (45%) Frame = +1 Query: 115 EHTLSIPTAVDEMEKKELRTSATETGLNSKIGIRGTDRLEILSNVAVFDESLSDTGGREK 294 E++L+ +++ E TS TETG ++K +E N AVFDE + + Sbjct: 1258 ENSLNEAVFDEKIPGSEASTSTTETGPHNKTFPEEPFAMENFLNEAVFDEKIPGSEAPVS 1317 Query: 295 CLLRAHKSVSLTEETGVLPMPTDL 366 + + TEE P+ TD+ Sbjct: 1318 TTETGLHNETFTEE----PVATDI 1337 >At2g39630.1 68415.m04858 glycosyl transferase family 2 protein similar to dolichyl-phosphate beta-glucosyltransferase from Saccharomyces cerevisiae [SP|P40350]; contains Pfam glycosyltransferase group 2 domain PF00535 Length = 336 Score = 29.1 bits (62), Expect = 3.3 Identities = 17/48 (35%), Positives = 28/48 (58%) Frame = -3 Query: 323 DTDLCALNKHFSLPPVSLSDSSKTATLLKISSLSVPRIPIFELSPVSV 180 D +L L K F++P V +S K+S LS+P + ++EL+ +SV Sbjct: 278 DVELVYLCKRFNIPMVEISVKWSEIPGSKVSMLSIPNM-LWELALMSV 324 >At5g15630.1 68418.m01829 phytochelatin synthetase family protein / COBRA cell expansion protein COBL4 similar to phytochelatin synthetase [Hordeum vulgare subsp. vulgare] GI:29570314; identified in Roudier, et al, Plant Phys. (2002) 130:538-548 (PMID:12376623); contains Pfam profile PF04833: Phytochelatin synthetase-like conserved region Length = 431 Score = 28.7 bits (61), Expect = 4.4 Identities = 12/30 (40%), Positives = 22/30 (73%), Gaps = 2/30 (6%) Frame = -3 Query: 707 VNNTDWSCILEHHNINDM--VNSFNYKVIS 624 +N+T W+ ++H N+N++ V SF+YK +S Sbjct: 305 MNHTLWTLAIQHPNLNNVTQVFSFDYKPVS 334 >At5g49110.1 68418.m06079 expressed protein ; expression supported by MPSS Length = 1487 Score = 28.3 bits (60), Expect = 5.8 Identities = 22/67 (32%), Positives = 35/67 (52%) Frame = -3 Query: 293 FSLPPVSLSDSSKTATLLKISSLSVPRIPIFELSPVSVADVRNSFFSISSTAVGIDNVCS 114 FSLPP LS SS + L + S S P ++S +A + S S+S T ++CS Sbjct: 33 FSLPPYLLSPSSHDSLLSYLKSRSSSPSPSKQVSEYVIALL--SLISLSPTT---PSLCS 87 Query: 113 KMIALIL 93 + +L++ Sbjct: 88 LLASLLI 94 >At5g23050.1 68418.m02695 acyl-activating enzyme 17 (AAE17) nearly identical to acyl-activating enzyme 17 [Arabidopsis thaliana] GI:29893266; similar to acetyl-CoA synthetase [SP|P27095] from Methanothrix soehngenii; contains Pfam AMP-binding enzyme domain PF00501; identical to cDNA acyl-activating enzyme 17 (At5g23050) GI:29893265 Length = 721 Score = 28.3 bits (60), Expect = 5.8 Identities = 13/28 (46%), Positives = 21/28 (75%), Gaps = 1/28 (3%) Frame = +1 Query: 127 SIP-TAVDEMEKKELRTSATETGLNSKI 207 S+P TA +++ ++ LR T+TGLNSK+ Sbjct: 694 SLPRTATNKVMRRVLRQQLTQTGLNSKL 721 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,026,808 Number of Sequences: 28952 Number of extensions: 329670 Number of successful extensions: 892 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 875 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 892 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1672953192 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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