BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= cesb0024 (892 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi... 166 3e-42 SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch... 166 3e-42 SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|... 106 4e-24 SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz... 106 4e-24 SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M... 106 4e-24 SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like pro... 67 3e-12 SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pomb... 39 0.001 SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc... 38 0.002 SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase Ubp6|Schizosacc... 36 0.006 SPAC227.15 |||protein phosphatase regulatory subunit Reg1 |Schiz... 28 1.6 SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 27 2.7 SPAC29B12.06c |rcd1||RNA-binding protein Rcd1 |Schizosaccharomyc... 27 4.7 >SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 166 bits (404), Expect = 3e-42 Identities = 82/132 (62%), Positives = 96/132 (72%) Frame = -2 Query: 891 VEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVXXXXXX 712 VE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 17 VESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76 Query: 711 XXXXXXKNYSTPXXXXXXXXXXKLAVLRFYKVDENGKIHRLRRECTGEQCGAGVFMAVME 532 K Y+TP +LAVL++YKV+++G + RLRREC CGA FMA + Sbjct: 77 GKKRKKKTYTTPKKIKHKHKKVELAVLKYYKVEDDGSVKRLRREC--PNCGASTFMANHK 134 Query: 531 DRHYCGKCHSTM 496 DR YCG+CH T+ Sbjct: 135 DRLYCGRCHLTL 146 >SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 166 bits (404), Expect = 3e-42 Identities = 82/132 (62%), Positives = 96/132 (72%) Frame = -2 Query: 891 VEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVXXXXXX 712 VE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 17 VESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76 Query: 711 XXXXXXKNYSTPXXXXXXXXXXKLAVLRFYKVDENGKIHRLRRECTGEQCGAGVFMAVME 532 K Y+TP +LAVL++YKV+++G + RLRREC CGA FMA + Sbjct: 77 GKKRKKKTYTTPKKIKHKHKKVELAVLKYYKVEDDGSVKRLRREC--PNCGASTFMANHK 134 Query: 531 DRHYCGKCHSTM 496 DR YCG+CH T+ Sbjct: 135 DRLYCGRCHLTL 146 >SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 106 bits (255), Expect = 4e-24 Identities = 50/54 (92%), Positives = 53/54 (98%) Frame = -2 Query: 891 VEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 730 VE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 17 VESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 70 >SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 106 bits (255), Expect = 4e-24 Identities = 50/54 (92%), Positives = 53/54 (98%) Frame = -2 Query: 891 VEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 730 VE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 17 VESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 70 >SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||Manual Length = 382 Score = 106 bits (255), Expect = 4e-24 Identities = 50/54 (92%), Positives = 53/54 (98%) Frame = -2 Query: 891 VEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 730 VE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 17 VESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 70 Score = 106 bits (255), Expect = 4e-24 Identities = 50/54 (92%), Positives = 53/54 (98%) Frame = -2 Query: 891 VEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 730 VE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 93 VESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 146 Score = 106 bits (255), Expect = 4e-24 Identities = 50/54 (92%), Positives = 53/54 (98%) Frame = -2 Query: 891 VEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 730 VE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 169 VESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 222 Score = 106 bits (255), Expect = 4e-24 Identities = 50/54 (92%), Positives = 53/54 (98%) Frame = -2 Query: 891 VEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 730 VE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 245 VESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 298 Score = 106 bits (255), Expect = 4e-24 Identities = 50/54 (92%), Positives = 53/54 (98%) Frame = -2 Query: 891 VEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 730 VE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 321 VESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 374 >SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like protein modifier Ned8|Schizosaccharomyces pombe|chr 2|||Manual Length = 78 Score = 67.3 bits (157), Expect = 3e-12 Identities = 25/54 (46%), Positives = 41/54 (75%) Frame = -2 Query: 891 VEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 730 ++P+D + +K ++++KEGIPP+QQRLI+AGKQ+ D + Y+++ S LHLV Sbjct: 17 IDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYHLEGGSVLHLV 70 >SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pombe|chr 2|||Manual Length = 368 Score = 38.7 bits (86), Expect = 0.001 Identities = 17/46 (36%), Positives = 32/46 (69%), Gaps = 1/46 (2%) Frame = -2 Query: 882 SDT-IENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKE 748 +DT I +K KIQ ++ +Q+LI++G+ L D +T+ +YNI+++ Sbjct: 20 ADTKISELKEKIQTQQNYEVERQKLIYSGRILADDKTVGEYNIKEQ 65 >SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces pombe|chr 1|||Manual Length = 354 Score = 37.9 bits (84), Expect = 0.002 Identities = 19/54 (35%), Positives = 30/54 (55%) Frame = -2 Query: 891 VEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 730 V+ ++ +K I I +QRLI+AG+ L+D +L Y IQ ++HLV Sbjct: 20 VDSESSVLALKEAIAPVADIEKERQRLIYAGRVLKDEESLKTYKIQDGHSIHLV 73 >SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase Ubp6|Schizosaccharomyces pombe|chr 1|||Manual Length = 467 Score = 36.3 bits (80), Expect = 0.006 Identities = 16/51 (31%), Positives = 29/51 (56%) Frame = -2 Query: 891 VEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTL 739 +EP++T +K ++ +PP +Q++I G QL+D L I+ +TL Sbjct: 17 IEPNETGSTLKHQLYSLTQVPPERQKVIVKGGQLKDDVLLGSVGIKPNATL 67 >SPAC227.15 |||protein phosphatase regulatory subunit Reg1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 873 Score = 28.3 bits (60), Expect = 1.6 Identities = 14/35 (40%), Positives = 18/35 (51%) Frame = +1 Query: 418 NPSYTAVASVPYRLEGTYLFVVVFEDHRAMTLPAV 522 NP+YT Y L TYL F++HR + AV Sbjct: 144 NPAYTNSHQNSYSLNETYLSYDFFDNHRGASSSAV 178 >SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 903 Score = 27.5 bits (58), Expect = 2.7 Identities = 10/29 (34%), Positives = 17/29 (58%) Frame = +3 Query: 750 PSGYCSPRGYVHLPAAFQQRSTFVDWEGF 836 PS + PRG V+ P+ ++R + + GF Sbjct: 17 PSSFLKPRGLVYRPSQIRRRVSLLSLSGF 45 >SPAC29B12.06c |rcd1||RNA-binding protein Rcd1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 283 Score = 26.6 bits (56), Expect = 4.7 Identities = 14/54 (25%), Positives = 24/54 (44%), Gaps = 1/54 (1%) Frame = -2 Query: 636 VLRFY-KVDENGKIHRLRRECTGEQCGAGVFMAVMEDRHYCGKCHSTMVFKDDD 478 V+R Y ++ +N + R C E F V++D H KC + ++ D Sbjct: 224 VIRCYLRLSDNPRAREALRHCLPEPLRDATFAQVLKDDHNTKKCLAQLLINLSD 277 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,373,540 Number of Sequences: 5004 Number of extensions: 67353 Number of successful extensions: 163 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 153 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 161 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 448490560 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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