Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ceN-6422
         (651 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase Ub...    31   0.14 
SPAC23C11.04c |pnk1||DNA kinase/phosphatase Pnk1|Schizosaccharom...    29   0.58 
SPAC3F10.03 |||glycine tRNA-ligase|Schizosaccharomyces pombe|chr...    27   2.3  
SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyc...    27   3.1  
SPCC1753.03c |rec7||meiotic recombination protein Rec7 |Schizosa...    26   4.1  
SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces ...    26   5.4  
SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosacc...    26   5.4  
SPBC337.14 |rpb4||DNA-directed RNA polymerase II complex subunit...    26   5.4  

>SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase
           Ubp21|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1129

 Score = 31.1 bits (67), Expect = 0.14
 Identities = 13/43 (30%), Positives = 23/43 (53%)
 Frame = +2

Query: 245 DDEARSEATFRFTVHNFKNLKDSMLSPPCYVRNLPWKIMVMPR 373
           D E  + A++ + V NF  L+D   SP     +  W+I++ P+
Sbjct: 45  DLEEAAHASYSWVVKNFSTLEDKTYSPLFKAGHTTWRIVLFPK 87


>SPAC23C11.04c |pnk1||DNA kinase/phosphatase
           Pnk1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 421

 Score = 29.1 bits (62), Expect = 0.58
 Identities = 11/34 (32%), Positives = 19/34 (55%)
 Frame = +2

Query: 209 EMPLACLDAEMEDDEARSEATFRFTVHNFKNLKD 310
           E+P+ C+  +  ++ AR    FR+  HN K L +
Sbjct: 346 EIPIRCIHLQSSEELARHNNVFRYIHHNQKQLPE 379


>SPAC3F10.03 |||glycine tRNA-ligase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 652

 Score = 27.1 bits (57), Expect = 2.3
 Identities = 16/48 (33%), Positives = 25/48 (52%)
 Frame = +2

Query: 80  VEMETQDVETITTEKTWNEEMMKGPNGEVVMADVMKTQETASAEMPLA 223
           V+ ET   ETIT  +  + + ++G   EV+ A V   +  +S E  LA
Sbjct: 595 VDFETLQNETITLRERDSTKQVRGSQDEVIAALVSMVEGKSSFEDALA 642


>SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 259

 Score = 26.6 bits (56), Expect = 3.1
 Identities = 10/38 (26%), Positives = 21/38 (55%)
 Frame = +2

Query: 119 EKTWNEEMMKGPNGEVVMADVMKTQETASAEMPLACLD 232
           EK W E + +     + ++ +++  +TAS  + LA +D
Sbjct: 175 EKNWKEGLSRDETIHLAVSSLLEVVQTASGNIELAIMD 212


>SPCC1753.03c |rec7||meiotic recombination protein Rec7
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 339

 Score = 26.2 bits (55), Expect = 4.1
 Identities = 13/50 (26%), Positives = 28/50 (56%), Gaps = 2/50 (4%)
 Frame = +2

Query: 2   LVPNSARGNHTPAPDRQQHDSNVNQVVEMETQDVETITT--EKTWNEEMM 145
           ++PN+   ++TP   R + D+ + Q+       +E+IT   + TW+E ++
Sbjct: 192 VMPNAEIFSNTPPLKRFRGDAGMTQMPLRSDTSIESITASQQPTWDENVV 241


>SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 444

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 10/37 (27%), Positives = 20/37 (54%)
 Frame = +2

Query: 77  VVEMETQDVETITTEKTWNEEMMKGPNGEVVMADVMK 187
           V++M+T D+  +  +  +NE  +  P   V  + +MK
Sbjct: 73  VIDMDTIDITNLNRQFLFNESNIDEPKANVAASMIMK 109


>SPAC10F6.02c |prp22||ATP-dependent RNA helicase
           Prp22|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1168

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 10/27 (37%), Positives = 17/27 (62%)
 Frame = -3

Query: 181 NISHDNLTIRTLHHFFIPGFFSGNCFN 101
           NI+  +LTI  +++   PGF   +CF+
Sbjct: 786 NIAETSLTIDGIYYVVDPGFVKQSCFD 812


>SPBC337.14 |rpb4||DNA-directed RNA polymerase II complex subunit
           Rpb4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 135

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 4/39 (10%)
 Frame = +2

Query: 83  EMETQDVETITTEKTWNEEMM----KGPNGEVVMADVMK 187
           E E +D+ T++  K   E ++    +  NGE+ M DVMK
Sbjct: 18  EFENEDMLTVSEAKILIETVLAQRARETNGEIPMTDVMK 56


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,516,339
Number of Sequences: 5004
Number of extensions: 48262
Number of successful extensions: 133
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 126
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 133
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)