BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-6053
(701 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC25B2.11 |pof2||F-box protein Pof2|Schizosaccharomyces pombe|... 27 2.6
SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme ... 27 3.4
SPAC4F8.11 |||WD repeat protein, human WDR24 family|Schizosaccha... 27 3.4
SPBC354.15 |fap1||L-pipecolate oxidase|Schizosaccharomyces pombe... 26 4.5
SPBC4C3.06 |||actin cytoskeletal protein Syp1|Schizosaccharomyce... 26 6.0
SPBC24C6.05 |sec28||coatomer epsilon subunit |Schizosaccharomyce... 25 7.9
SPAC27F1.08 |pdt1||Nramp family manganese ion transporter|Schizo... 25 7.9
SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 25 7.9
>SPBC25B2.11 |pof2||F-box protein Pof2|Schizosaccharomyces pombe|chr
2|||Manual
Length = 463
Score = 27.1 bits (57), Expect = 2.6
Identities = 14/46 (30%), Positives = 25/46 (54%)
Frame = -3
Query: 372 VNVINVNLHFNHNMYFVRNYYTLDRLLKLTVLPMYNCKFLRNFALT 235
++VI ++ F+ N+ V Y + K MYNCK L++ ++T
Sbjct: 370 LSVILLSGSFSRNLERVHLSYCIGLTAKSVSYLMYNCKTLKHLSVT 415
>SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1012
Score = 26.6 bits (56), Expect = 3.4
Identities = 18/68 (26%), Positives = 34/68 (50%)
Frame = -1
Query: 269 TIVNFLEISH*HISTNKLKEILLFPLNNFTCHDFCLRSVSSATKELEIVFSKHPNYYNLF 90
T+ E++ ++ + + E+ L NF C + + +S TK+LEI H N+
Sbjct: 96 TVSKLAELNQ-YVPVSVVDELSTEYLKNFKC---VVVTETSLTKQLEINDFTHKNHIAYI 151
Query: 89 SLDNIGVF 66
+ D+ G+F
Sbjct: 152 AADSRGLF 159
>SPAC4F8.11 |||WD repeat protein, human WDR24
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 846
Score = 26.6 bits (56), Expect = 3.4
Identities = 15/43 (34%), Positives = 23/43 (53%), Gaps = 1/43 (2%)
Frame = -1
Query: 191 NNFTCHDFCLRSVSSATKELEIVFSKHP-NYYNLFSLDNIGVF 66
N F C D R+ SS + + +VFS +P + L SL+ + F
Sbjct: 345 NAFNCIDMLPRATSSWSTKNSLVFSSNPISNQRLSSLNRVASF 387
>SPBC354.15 |fap1||L-pipecolate oxidase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 412
Score = 26.2 bits (55), Expect = 4.5
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -1
Query: 143 TKELEIVFSKHPNYYNLF 90
T + E VF HP+Y NLF
Sbjct: 334 TADAEFVFDYHPDYENLF 351
>SPBC4C3.06 |||actin cytoskeletal protein Syp1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 818
Score = 25.8 bits (54), Expect = 6.0
Identities = 11/19 (57%), Positives = 14/19 (73%)
Frame = +2
Query: 578 YSRKILKKKLHIYNANIST 634
+SRK K KLH+ N NIS+
Sbjct: 715 FSRKTSKLKLHLSNINISS 733
>SPBC24C6.05 |sec28||coatomer epsilon subunit |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 288
Score = 25.4 bits (53), Expect = 7.9
Identities = 11/36 (30%), Positives = 12/36 (33%)
Frame = -1
Query: 194 LNNFTCHDFCLRSVSSATKELEIVFSKHPNYYNLFS 87
L C D CL A L+ PNY S
Sbjct: 199 LTGMACADICLLRPEEALSSLKTALDSQPNYEEALS 234
>SPAC27F1.08 |pdt1||Nramp family manganese ion
transporter|Schizosaccharomyces pombe|chr 1|||Manual
Length = 521
Score = 25.4 bits (53), Expect = 7.9
Identities = 13/28 (46%), Positives = 15/28 (53%)
Frame = -1
Query: 218 LKEILLFPLNNFTCHDFCLRSVSSATKE 135
L L FPL FTC +R VS +T E
Sbjct: 448 LLPFLTFPLVMFTCSRKVMRVVSDSTNE 475
>SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 486
Score = 25.4 bits (53), Expect = 7.9
Identities = 11/29 (37%), Positives = 14/29 (48%)
Frame = -1
Query: 179 CHDFCLRSVSSATKELEIVFSKHPNYYNL 93
CHD CL S T L + P ++NL
Sbjct: 110 CHDDCLSSSPPCTSALTEITLLPPTFHNL 138
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,209,239
Number of Sequences: 5004
Number of extensions: 35164
Number of successful extensions: 81
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 79
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 81
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 325165428
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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