BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-5855
(672 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC17G9.13c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 30 0.26
SPBP8B7.15c |||ubiquitin-protein ligase E3 RBBP6 family |Schizos... 26 4.3
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 26 5.7
SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosacchar... 25 9.9
SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 25 9.9
SPBC1347.07 |rex2||RNA exonuclease|Schizosaccharomyces pombe|chr... 25 9.9
SPBC8D2.01 |gsk31||serine/threonine protein kinase Gsk31|Schizos... 25 9.9
SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |... 25 9.9
>SPBC17G9.13c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 317
Score = 30.3 bits (65), Expect = 0.26
Identities = 13/27 (48%), Positives = 18/27 (66%)
Frame = -2
Query: 380 RWPLACTGPARPRRSHYLQSHEVSYSK 300
R+ + TGP R RR HYLQ +++ SK
Sbjct: 126 RYAYSQTGPLRHRRLHYLQDNKIRPSK 152
>SPBP8B7.15c |||ubiquitin-protein ligase E3 RBBP6 family
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 482
Score = 26.2 bits (55), Expect = 4.3
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = +3
Query: 498 SAVPQYRPQIRS**AAQ*DEPPPP 569
SA P Y+P R A+ D+PPPP
Sbjct: 158 SATPIYKPNQRRIAASVPDKPPPP 181
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 25.8 bits (54), Expect = 5.7
Identities = 11/29 (37%), Positives = 14/29 (48%)
Frame = +2
Query: 209 HLLEVTEQQQETLVPVVSASTPPRKPDEQ 295
HLLE + +P +S PPR D Q
Sbjct: 547 HLLEERIHSKRANIPTISVIPPPRSMDAQ 575
>SPAC26A3.09c |rga2||GTPase activating protein
Rga2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1275
Score = 25.0 bits (52), Expect = 9.9
Identities = 19/46 (41%), Positives = 21/46 (45%)
Frame = +3
Query: 492 PYSAVPQYRPQIRS**AAQ*DEPPPPYRSSGSSTGRTWWCPSGSPR 629
P S VP Y R + PPPP SS S+T R PS PR
Sbjct: 402 PTSNVPAYSTPARPT-----ESPPPPPISSSSTTPRPDDKPSLPPR 442
>SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 425
Score = 25.0 bits (52), Expect = 9.9
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = -1
Query: 120 LKHECNEVSSSSDCINVLYRDL 55
L H+CN S + CI+ +Y+ L
Sbjct: 362 LGHKCNSCHSYNTCISSIYKPL 383
>SPBC1347.07 |rex2||RNA exonuclease|Schizosaccharomyces pombe|chr
2|||Manual
Length = 180
Score = 25.0 bits (52), Expect = 9.9
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = +2
Query: 461 LADVTHAERVALLSCPPIPAADKELISGAVRRAAAAISELR 583
+ DV+ + +A CP IPA DK+ A+ +I EL+
Sbjct: 132 IIDVSTIKELAKRWCPDIPAYDKKGDHRALSDILESIGELQ 172
>SPBC8D2.01 |gsk31||serine/threonine protein kinase
Gsk31|Schizosaccharomyces pombe|chr 2|||Manual
Length = 381
Score = 25.0 bits (52), Expect = 9.9
Identities = 10/33 (30%), Positives = 21/33 (63%), Gaps = 1/33 (3%)
Frame = +2
Query: 158 ICHKDNDPQENVINERTHLLEVTE-QQQETLVP 253
+CH+D PQ +++ +T +L++ + + LVP
Sbjct: 146 VCHRDIKPQNLLVDYKTGILKLCDFGSAKVLVP 178
>SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 499
Score = 25.0 bits (52), Expect = 9.9
Identities = 14/46 (30%), Positives = 19/46 (41%)
Frame = +2
Query: 236 QETLVPVVSASTPPRKPDEQTALNRILHETANNVIDVGALGPYTLE 373
Q+T V AST E A + HE +G P++LE
Sbjct: 273 QDTFFLKVPASTDKLPDPEYVARVKATHENGGETKGIGYRAPFSLE 318
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,187,578
Number of Sequences: 5004
Number of extensions: 39116
Number of successful extensions: 156
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 151
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 156
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 307866294
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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