Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ceN-5837
         (619 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3F10.03 |||glycine tRNA-ligase|Schizosaccharomyces pombe|chr...    35   0.011
SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|ch...    32   0.076
SPAC458.02c |||mRNP complex |Schizosaccharomyces pombe|chr 1|||M...    31   0.18 
SPBC887.03c |noc3||Noc2p-Noc3p complex subunit Noc3 |Schizosacch...    30   0.31 
SPAC23C11.16 |plo1||Polo kinase Plo1|Schizosaccharomyces pombe|c...    28   1.2  
SPCC1393.07c |mug4||sequence orphan|Schizosaccharomyces pombe|ch...    27   2.2  
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit...    27   2.9  
SPAC15A10.13 |ppk3||serine/threonine protein kinase Ppk3|Schizos...    27   2.9  
SPAC688.12c |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    27   2.9  
SPAC1952.14c |mrpl25||mitochondrial ribosomal protein subunit L2...    27   2.9  
SPAC2F7.14c |||exosome subunit Rrp4 |Schizosaccharomyces pombe|c...    26   3.8  
SPBC1539.10 |||ribosome biogenesis protein Nop16 |Schizosaccharo...    26   5.0  
SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces po...    25   6.6  
SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase Ubp6|Schizosacc...    25   6.6  
SPBC31E1.05 |gle1||RNA export factor Gle1 |Schizosaccharomyces p...    25   6.6  

>SPAC3F10.03 |||glycine tRNA-ligase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 652

 Score = 34.7 bits (76), Expect = 0.011
 Identities = 13/23 (56%), Positives = 18/23 (78%)
 Frame = +3

Query: 549 FDRAKMEDLIKRRFFYDQSFAIY 617
           FDR + E+L+K+RFF+  SF IY
Sbjct: 10  FDRTQFEELMKKRFFFSPSFQIY 32


>SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 538

 Score = 31.9 bits (69), Expect = 0.076
 Identities = 16/41 (39%), Positives = 23/41 (56%)
 Frame = +3

Query: 384 LAPLRANVKEQGDLVRKLKEEKAPEIDIKKAVAELKTRKKI 506
           L  +   +  QG+LVR LK + A + DI K VA+L   K +
Sbjct: 5   LVDINERITSQGNLVRSLKSQGASKEDIDKEVAKLLQLKNL 45


>SPAC458.02c |||mRNP complex |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 468

 Score = 30.7 bits (66), Expect = 0.18
 Identities = 23/83 (27%), Positives = 47/83 (56%), Gaps = 1/83 (1%)
 Frame = +3

Query: 297 WGSNKLHRKIKIPNPFREIIMADPKIEEILAPLRANVKEQGDLVRKLKEEKAPEIDIKKA 476
           +G+ K    + +P PF +    D  +E++ + + A+ KEQ D V K + EKA + +I+K 
Sbjct: 375 FGTIKEFDFVGVPAPFTKS-QVDSAVEQLKSRI-AHFKEQQDSVTKQRIEKAKQ-EIEKL 431

Query: 477 VAELKTR-KKILEDKELSLAPAE 542
            A+  ++ +K L + ++ ++  E
Sbjct: 432 EAKYNSKEEKTLTEADMVISSEE 454


>SPBC887.03c |noc3||Noc2p-Noc3p complex subunit Noc3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 747

 Score = 29.9 bits (64), Expect = 0.31
 Identities = 24/96 (25%), Positives = 44/96 (45%), Gaps = 2/96 (2%)
 Frame = +3

Query: 273 LRLSHSQQWGSNKLHR-KIKIPNPFREIIMADPKI-EEILAPLRANVKEQGDLVRKLKEE 446
           LR   S  W +   +R  IK  +   + ++AD    EE L+   +    + D    +K+E
Sbjct: 140 LRPRVSSSWNNESYNRLPIKTKDGLLQNVVADVNNGEEFLSESESEASLEID--SDIKDE 197

Query: 447 KAPEIDIKKAVAELKTRKKILEDKELSLAPAEDLFD 554
           K   ++ +K   E+  +++I  DKE     A+ L +
Sbjct: 198 KQKSLEEQKIAPEIPVKQQIKNDKEALGIQAQQLLE 233


>SPAC23C11.16 |plo1||Polo kinase Plo1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 683

 Score = 27.9 bits (59), Expect = 1.2
 Identities = 15/42 (35%), Positives = 23/42 (54%)
 Frame = +3

Query: 315 HRKIKIPNPFREIIMADPKIEEILAPLRANVKEQGDLVRKLK 440
           HRK+ I +  R+II+ D + E +  PL+       DL  +LK
Sbjct: 626 HRKVVISSTARKIIVLDKERERVELPLQEASAFSEDLRSRLK 667


>SPCC1393.07c |mug4||sequence orphan|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 845

 Score = 27.1 bits (57), Expect = 2.2
 Identities = 13/30 (43%), Positives = 19/30 (63%), Gaps = 1/30 (3%)
 Frame = -1

Query: 427 TKSPCSFTLALKGARISSILG-SAIMISRK 341
           T SPCSFT+    AR+ S+ G S + + R+
Sbjct: 60  TLSPCSFTICSLRARLHSLHGPSTVFLKRE 89


>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
            hand and WH2 motif |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1794

 Score = 26.6 bits (56), Expect = 2.9
 Identities = 23/52 (44%), Positives = 28/52 (53%), Gaps = 12/52 (23%)
 Frame = +3

Query: 429  RKLKEEK---APEI--DIKKAVAELKTR---KKI----LEDKELSLAPAEDL 548
            R  KEEK   AP I  DI   +A LK R   K +    LEDK+L+  PA D+
Sbjct: 977  RDFKEEKIESAPPIINDIDSEIASLKKRIHEKSLVVNALEDKKLAATPANDV 1028


>SPAC15A10.13 |ppk3||serine/threonine protein kinase
           Ppk3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 637

 Score = 26.6 bits (56), Expect = 2.9
 Identities = 14/51 (27%), Positives = 23/51 (45%)
 Frame = +3

Query: 375 EEILAPLRANVKEQGDLVRKLKEEKAPEIDIKKAVAELKTRKKILEDKELS 527
           E + AP + N       +       +P ID+KK   ELK     +++K+ S
Sbjct: 537 ENVSAPAKFNGSFWSKFIHSSSASPSPSIDMKKESLELKNDTTEIKEKKNS 587


>SPAC688.12c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 167

 Score = 26.6 bits (56), Expect = 2.9
 Identities = 12/34 (35%), Positives = 21/34 (61%)
 Frame = +3

Query: 348 EIIMADPKIEEILAPLRANVKEQGDLVRKLKEEK 449
           E+  A+P+ E +LA  +  +KEQ +   + KE+K
Sbjct: 121 EVACANPEDEALLAERKQRLKEQREKKEQKKEQK 154


>SPAC1952.14c |mrpl25||mitochondrial ribosomal protein subunit
           L25|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 148

 Score = 26.6 bits (56), Expect = 2.9
 Identities = 16/50 (32%), Positives = 28/50 (56%)
 Frame = +3

Query: 468 KKAVAELKTRKKILEDKELSLAPAEDLFDRAKMEDLIKRRFFYDQSFAIY 617
           K  ++E K R +ILE++ L L+ +     + K E + K  +F+D   +IY
Sbjct: 100 KGKISERK-RDEILENRRLKLSESLKKTSKFKNERVCKLLYFHDFLISIY 148


>SPAC2F7.14c |||exosome subunit Rrp4 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 329

 Score = 26.2 bits (55), Expect = 3.8
 Identities = 17/58 (29%), Positives = 33/58 (56%), Gaps = 6/58 (10%)
 Frame = +3

Query: 423 LVRKLKEEK---APEIDIKKAVAELKTRKKILEDKELSLAPAEDL---FDRAKMEDLI 578
           +V  LK E+   + E DI   V+  +   +I+ED+++ L   ED+   FD++  ++L+
Sbjct: 1   MVTILKPEEFYVSSEADIVNDVSMTEMEDEIMEDEQMGLVDGEDVLEEFDKSIHQNLV 58


>SPBC1539.10 |||ribosome biogenesis protein Nop16
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 209

 Score = 25.8 bits (54), Expect = 5.0
 Identities = 14/34 (41%), Positives = 21/34 (61%)
 Frame = +3

Query: 483 ELKTRKKILEDKELSLAPAEDLFDRAKMEDLIKR 584
           E KT   +L DKE+S+APA+    R   E+ +K+
Sbjct: 116 EAKTLDDVL-DKEISIAPAKTEVVRQLEEEAVKK 148


>SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces
            pombe|chr 3|||Manual
          Length = 2812

 Score = 25.4 bits (53), Expect = 6.6
 Identities = 14/38 (36%), Positives = 18/38 (47%)
 Frame = +3

Query: 486  LKTRKKILEDKELSLAPAEDLFDRAKMEDLIKRRFFYD 599
            L +  K+   KE SL+    L    +MEDL  R   YD
Sbjct: 2544 LDSAHKVYHPKEWSLSTCRKLIAEKQMEDLETRLKVYD 2581


>SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase
           Ubp6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 467

 Score = 25.4 bits (53), Expect = 6.6
 Identities = 19/66 (28%), Positives = 31/66 (46%), Gaps = 5/66 (7%)
 Frame = +3

Query: 309 KLHRKIKIPNPFREIIMADPKIEEILAPLRANVKE----QGDLVRKLKEEK-APEIDIKK 473
           K+ RK+K P     +    P++ + L P+R  ++E      +  R  K  K  P  D K+
Sbjct: 305 KILRKVKFPFELDAVEFCTPELSQKLIPVRDKLREIEKNDEEHERAAKRIKIQPSEDEKE 364

Query: 474 AVAELK 491
           A AE +
Sbjct: 365 AEAECR 370


>SPBC31E1.05 |gle1||RNA export factor Gle1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 480

 Score = 25.4 bits (53), Expect = 6.6
 Identities = 19/64 (29%), Positives = 31/64 (48%)
 Frame = +3

Query: 411 EQGDLVRKLKEEKAPEIDIKKAVAELKTRKKILEDKELSLAPAEDLFDRAKMEDLIKRRF 590
           EQ    R+L E+K  E + ++   E + RKK L +KE        L +  K ++  +RR 
Sbjct: 109 EQERFNRELLEKKRIEAERQRLKDEEERRKKELMEKEKKEKERIRLIEEQKHKENEQRRL 168

Query: 591 FYDQ 602
             +Q
Sbjct: 169 KQEQ 172


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,081,513
Number of Sequences: 5004
Number of extensions: 36955
Number of successful extensions: 98
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 97
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 98
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 271646730
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)