Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-5668
(652 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 259 2e-70
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 255 5e-69
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 106 2e-24
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 53 3e-08
SPBC16A3.01 |spn3|SPBC543.01c|septin Spn3|Schizosaccharomyces po... 29 0.77
SPAC1142.04 |||Noc2p-Noc3p complex subunit Noc2 family |Schizosa... 27 3.1
SPAC890.05 |||ribosome biogenesis protein|Schizosaccharomyces po... 27 3.1
SPBC725.17c |rrn11||RNA polymerase I transcription factor subuni... 26 5.4
SPAC17A5.09c |||protein phosphatase regulatory subunit Glc9|Schi... 25 7.2
SPCC16C4.09 |sts5|orb4|RNB-like protein|Schizosaccharomyces pomb... 25 9.5
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 259 bits (635), Expect = 2e-70
Identities = 113/142 (79%), Positives = 129/142 (90%)
Frame = +3
Query: 138 MVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPT 317
MVKCDPR G+YMA C+LYRGDV+P+DV AA+ +IK++RTIQFVDWCPTGFK+GI Y+PP
Sbjct: 306 MVKCDPRTGRYMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQ 365
Query: 318 VVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEA 497
VPG +AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEA
Sbjct: 366 HVPGSGIAKVNRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEA 425
Query: 498 REDLAALEKDYEEVGVDSTEGE 563
REDLAALE+DYEEVG DS + E
Sbjct: 426 REDLAALERDYEEVGQDSMDNE 447
Score = 79.8 bits (188), Expect = 3e-16
Identities = 33/45 (73%), Positives = 38/45 (84%)
Frame = +2
Query: 2 TNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNACFEPANQ 136
TNLVPYPRIHFPL TY+P++SA KA+HE +V EITN CFEP NQ
Sbjct: 261 TNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQ 305
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 255 bits (624), Expect = 5e-69
Identities = 112/143 (78%), Positives = 128/143 (89%)
Frame = +3
Query: 138 MVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPT 317
MVKCDPR G+YMA C+LYRGDV+P+DV AA+ TIK KRTIQFVDWCPTGFK+GI +PP
Sbjct: 302 MVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQ 361
Query: 318 VVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEA 497
+ G ++AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEA
Sbjct: 362 HIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEA 421
Query: 498 REDLAALEKDYEEVGVDSTEGEF 566
REDLAALE+DYEEVG DS E ++
Sbjct: 422 REDLAALERDYEEVGQDSMEVDY 444
Score = 81.0 bits (191), Expect = 1e-16
Identities = 34/45 (75%), Positives = 38/45 (84%)
Frame = +2
Query: 2 TNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNACFEPANQ 136
TNLVPYPRIHFPL TYAP++SA KA+HE +V EITN CFEP NQ
Sbjct: 257 TNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQ 301
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 106 bits (255), Expect = 2e-24
Identities = 53/146 (36%), Positives = 84/146 (57%), Gaps = 3/146 (2%)
Frame = +3
Query: 138 MVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPT 317
MV DPRHG+Y+ L+RG V K+V+ I +++TK + FV+W P + PP
Sbjct: 300 MVAADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPK 359
Query: 318 VVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEA 497
DL + + + N+T+I E + RL +F M+ ++AF+HWY GEGM+E EF+EA
Sbjct: 360 -----DL---KMSATFIGNSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEA 411
Query: 498 R---EDLAALEKDYEEVGVDSTEGEF 566
DL + + Y+E G+D + ++
Sbjct: 412 ESNMNDLVSEYQQYQEAGIDEGDEDY 437
Score = 37.5 bits (83), Expect = 0.002
Identities = 14/43 (32%), Positives = 27/43 (62%)
Frame = +2
Query: 5 NLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNACFEPAN 133
N+VP+PR+HF + +AP+ + + + ++V E+T F+ N
Sbjct: 256 NMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFDANN 298
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 53.2 bits (122), Expect = 3e-08
Identities = 34/136 (25%), Positives = 69/136 (50%), Gaps = 4/136 (2%)
Frame = +3
Query: 138 MVKCDP-RHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPP 314
MV +P + +++ + +G+ P DV+ ++ I+ +R F+ W P +V ++ + P
Sbjct: 306 MVSVNPSKKSCFISILDIIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALSKKSP 365
Query: 315 TVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEE---GE 485
+ ++ + ML+N T+IA + R ++D + + AF+ Y E + E E
Sbjct: 366 YIKTNHRVSGL-----MLANHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFEDDLNE 420
Query: 486 FSEAREDLAALEKDYE 533
F +R+ +A L +YE
Sbjct: 421 FDSSRDVVADLINEYE 436
Score = 28.3 bits (60), Expect = 1.0
Identities = 16/54 (29%), Positives = 25/54 (46%), Gaps = 3/54 (5%)
Frame = +2
Query: 5 NLVPYPRIHFPLATYAPVIS---AEKAYHEQLTVAEITNACFEPANQYGEM*PA 157
+L+P PR HF L +Y P + E + TV ++ P NQ + P+
Sbjct: 259 SLIPSPRCHFLLTSYTPFTNQQVEEAKAIRKTTVLDVMRRLLLPKNQMVSVNPS 312
>SPBC16A3.01 |spn3|SPBC543.01c|septin Spn3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 412
Score = 28.7 bits (61), Expect = 0.77
Identities = 30/116 (25%), Positives = 50/116 (43%), Gaps = 11/116 (9%)
Frame = -3
Query: 497 GFGELALLHTLTHVPV--HEC------ALSVHQVELV-VQPGPRLGDGGCVGQHAHGALH 345
G G A ++TL P+ H A SV VE+V Q L DG + H
Sbjct: 62 GLGRTAFINTLCEKPLIRHNNNFDPAEASSVSPVEIVPYQTDIILEDGTKINLTVLDTPH 121
Query: 344 LGEVSARHHCWGLVIDAHLETSWAPVHELDGSLGFDG--CDSRIHVLRHNVSSVQH 183
GE + + +++ ++E+ + V E + + + CD R+H L + +S H
Sbjct: 122 FGEAIDNENNFDIILQ-YIESQYDNVLEEESRIKRNARFCDDRVHALIYFISPTGH 176
>SPAC1142.04 |||Noc2p-Noc3p complex subunit Noc2 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 707
Score = 26.6 bits (56), Expect = 3.1
Identities = 12/37 (32%), Positives = 18/37 (48%), Gaps = 1/37 (2%)
Frame = +1
Query: 226 LSQPSKP-REPSSSWTGAQLVSRWASITNPQQWCRAE 333
+ QPSK R+P SW+ + WA + + W E
Sbjct: 417 IHQPSKDSRKPVQSWSYVHSLDFWARLLSQAAWLSRE 453
>SPAC890.05 |||ribosome biogenesis protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 284
Score = 26.6 bits (56), Expect = 3.1
Identities = 16/70 (22%), Positives = 30/70 (42%)
Frame = -3
Query: 386 DGGCVGQHAHGALHLGEVSARHHCWGLVIDAHLETSWAPVHELDGSLGFDGCDSRIHVLR 207
+G +G+ HG +H +VS + G+ A + W+ + E + G D + +
Sbjct: 40 NGNGLGEKQHGRIHNIKVSLKDDTLGIGAKATNDLEWSGLGEFNAIFGRLNGDESAYGVY 99
Query: 206 HNVSSVQHTT 177
+ VQ T
Sbjct: 100 AEKAKVQQLT 109
>SPBC725.17c |rrn11||RNA polymerase I transcription factor subunit
Rrn11 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 200
Score = 25.8 bits (54), Expect = 5.4
Identities = 14/42 (33%), Positives = 20/42 (47%), Gaps = 1/42 (2%)
Frame = +1
Query: 4 EPGAVPAHPLPARHVRARHLRGE-GLPRAANGRRDHQRVLRA 126
E +P PLP+R + RH+ L R+D+ R RA
Sbjct: 22 ESSVIPPIPLPSRRYKTRHIDALCSLMHLCLLRKDYPRASRA 63
>SPAC17A5.09c |||protein phosphatase regulatory subunit
Glc9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 310
Score = 25.4 bits (53), Expect = 7.2
Identities = 18/49 (36%), Positives = 26/49 (53%)
Frame = +3
Query: 465 EGMEEGEFSEAREDLAALEKDYEEVGVDSTEGEFGRRK*ILKRDEEQST 611
E MEE SEA E++ + D+ E+ + F K LKRDE +S+
Sbjct: 241 EAMEEEALSEAEENIPKKKPDFNEL---RKKHYFAMAK-PLKRDELESS 285
>SPCC16C4.09 |sts5|orb4|RNB-like protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1066
Score = 25.0 bits (52), Expect = 9.5
Identities = 14/49 (28%), Positives = 24/49 (48%)
Frame = +3
Query: 459 VGEGMEEGEFSEAREDLAALEKDYEEVGVDSTEGEFGRRK*ILKRDEEQ 605
V G E E + R+D + ++ + +D+ E +R I RD+EQ
Sbjct: 358 VWAGKLEKEENRRRKDPISTRGSFDNLRIDAVPFEVPQRSAIKARDDEQ 406
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,563,432
Number of Sequences: 5004
Number of extensions: 53623
Number of successful extensions: 179
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 171
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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