Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-5495
(682 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ... 172 5e-44
SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 160 1e-40
SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomy... 42 6e-05
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 39 6e-04
SPBC31F10.06c |sar1||ADP-ribosylation factor Sar1|Schizosaccharo... 36 0.005
SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosacchar... 34 0.022
SPBC1685.01 |pmp1||dual-specificity MAP kinase phosphatase Pmp1|... 29 0.82
SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces pombe... 27 1.9
SPAC9E9.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 27 2.5
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy... 26 4.4
SPAC869.10c |||proline specific permease |Schizosaccharomyces po... 26 4.4
SPBC27B12.12c |||CorA family magnesium ion transporter |Schizosa... 26 5.8
SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32 |Schi... 25 7.7
SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 25 7.7
>SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 407
Score = 172 bits (418), Expect = 5e-44
Identities = 91/222 (40%), Positives = 134/222 (60%)
Frame = +1
Query: 1 LLLGAGESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSNTIQSLVAILRAMPNLGIIYG 180
LLLGAG+SGK+TI+KQM++++ GF+ KQYR +++ N I SL +L AM N +
Sbjct: 78 LLLGAGDSGKTTIMKQMRLLYSPGFSQVVRKQYRVMIFENIISSLCLLLEAMDNSNVSLL 137
Query: 181 NRDRESDGKMVFDVIQRMEDTEPFSEELLAAMKRLWADSGVQECFGRSNEYQLNDSAKYF 360
+ + + V + EPFS E+ A+ L D+ ++ L D+ Y+
Sbjct: 138 PENEKY--RAVILRKHTSQPNEPFSPEIYEAVHALTLDTKLRTVQSCGTNLSLLDNFYYY 195
Query: 361 LDDLDRLGAKDYQPTEQDILRTRVKTTGIVEVHFSFKNLNFKLFDVGGQRSERKKWIHCF 540
D +DR+ Y P++QDIL R+KTTGI E F +++ FDVGGQRSER+KWIHCF
Sbjct: 196 QDHIDRIFDPQYIPSDQDILHCRIKTTGISEETFLLNRHHYRFFDVGGQRSERRKWIHCF 255
Query: 541 EDVTAIIFCVAMSEYDQVLHEDENNEPYARRV*SCFDSICNN 666
E+VTA++F V+++ YDQ L ED N+ + +DSICN+
Sbjct: 256 ENVTALLFLVSLAGYDQCLVED-NSGNQMQEALLLWDSICNS 296
>SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit
Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 354
Score = 160 bits (389), Expect = 1e-40
Identities = 89/228 (39%), Positives = 140/228 (61%), Gaps = 5/228 (2%)
Frame = +1
Query: 1 LLLGAGESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSNTIQSLVAILRAMPNLGIIYG 180
LLLGA +SGKSTI KQ+KI++++GF+ E+ + PV+ N ++S +++ + GI
Sbjct: 38 LLLGASDSGKSTISKQIKILNKNGFSQEEIMTFIPVIRRNLLESAKTLVKIIVQKGI--- 94
Query: 181 NRDRESDGKMVFDVIQRMEDT--EPFSEELLAAMKRLWADSGVQECFGRSNEYQLNDSAK 354
+ + G ++I++ T E + + A+ LW+ + V+ C N+ L DSA
Sbjct: 95 --NLDPLGTHNCEIIEKFNPTPGELINANIGQAITSLWSANSVRSCT-YGNDSVLIDSAP 151
Query: 355 YFLDDLDRLGAKDYQPTEQDILRTRVKTTGIVEVHFSFKNLNFKLFDVGGQRSERKKWIH 534
YF D + ++ Y PT DILR+R T GI E+ F+ +L ++FDVGGQR+ER+KWI+
Sbjct: 152 YFFSRADEICSRHYVPTIDDILRSRNSTLGISEISFTLDHLQIRMFDVGGQRTERRKWIY 211
Query: 535 CFEDVTAIIFCVAMSEYDQVLHEDENNEPYARRV---*SCFDSICNNQ 669
CFE+V +IIFCV++++YD+ L+ E P R+ S FDSI N+Q
Sbjct: 212 CFENVNSIIFCVSLNDYDKKLY--ERAAPERNRLVESISLFDSIINSQ 257
>SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 180
Score = 42.3 bits (95), Expect = 6e-05
Identities = 20/63 (31%), Positives = 35/63 (55%), Gaps = 2/63 (3%)
Frame = +1
Query: 424 TRVKTTGIVEVHFSFKNLNFKLFDVGGQRSERKKWIHCFEDVTAIIFCVAMSEYDQV--L 597
T + T G ++N++F ++DVGGQ R W H F++ IIF V ++ +++
Sbjct: 44 TTIPTIGFNVETVEYRNISFTVWDVGGQDKIRPLWRHYFQNTQGIIFVVDSNDRERISEA 103
Query: 598 HED 606
HE+
Sbjct: 104 HEE 106
>SPBC1539.08 |||ADP-ribosylation factor, Arf
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 39.1 bits (87), Expect = 6e-04
Identities = 17/55 (30%), Positives = 29/55 (52%)
Frame = +1
Query: 430 VKTTGIVEVHFSFKNLNFKLFDVGGQRSERKKWIHCFEDVTAIIFCVAMSEYDQV 594
+ T G ++KN+ F ++DVGGQ R W H F +IF V ++ +++
Sbjct: 50 IPTVGFNVETVTYKNIKFNVWDVGGQDKIRPLWRHYFTGTKGLIFVVDSADSNRI 104
>SPBC31F10.06c |sar1||ADP-ribosylation factor
Sar1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 190
Score = 35.9 bits (79), Expect = 0.005
Identities = 21/87 (24%), Positives = 36/87 (41%), Gaps = 1/87 (1%)
Frame = +1
Query: 337 LNDSAKYFLDDLDRLGAKDY-QPTEQDILRTRVKTTGIVEVHFSFKNLNFKLFDVGGQRS 513
+N AK LD G + D L T + N+ F FD+GG +
Sbjct: 17 VNKHAKMLFLGLDNAGKTTLLHMLKNDRLAVMQPTLHPTSEELAIGNVRFTTFDLGGHQQ 76
Query: 514 ERKKWIHCFEDVTAIIFCVAMSEYDQV 594
R+ W F +V I++ V +++++
Sbjct: 77 ARRLWRDYFPEVNGIVYLVDCCDFERL 103
>SPAC22F3.05c |alp41||ADP-ribosylation factor
Alp41|Schizosaccharomyces pombe|chr 1|||Manual
Length = 186
Score = 33.9 bits (74), Expect = 0.022
Identities = 14/34 (41%), Positives = 22/34 (64%)
Frame = +1
Query: 469 KNLNFKLFDVGGQRSERKKWIHCFEDVTAIIFCV 570
+ L F ++D+GGQ++ R W + FE AII+ V
Sbjct: 58 EGLRFTIWDIGGQKTLRNFWKNYFESTEAIIWVV 91
>SPBC1685.01 |pmp1||dual-specificity MAP kinase phosphatase
Pmp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 278
Score = 28.7 bits (61), Expect = 0.82
Identities = 17/65 (26%), Positives = 30/65 (46%)
Frame = +1
Query: 430 VKTTGIVEVHFSFKNLNFKLFDVGGQRSERKKWIHCFEDVTAIIFCVAMSEYDQVLHEDE 609
+ + + + F K LN + D ER WI ++IF +SEY Q++ ++
Sbjct: 162 ISRSACLMIAFIMKTLNLNVSDAYEYVKERSPWIG---PNMSLIF--QLSEYQQIIRKNS 216
Query: 610 NNEPY 624
+ PY
Sbjct: 217 SQGPY 221
>SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 972
Score = 27.5 bits (58), Expect = 1.9
Identities = 11/11 (100%), Positives = 11/11 (100%)
Frame = +1
Query: 463 SFKNLNFKLFD 495
SFKNLNFKLFD
Sbjct: 553 SFKNLNFKLFD 563
>SPAC9E9.05 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 313
Score = 27.1 bits (57), Expect = 2.5
Identities = 15/48 (31%), Positives = 29/48 (60%), Gaps = 2/48 (4%)
Frame = +2
Query: 308 SALVVPTSTNSTTPLNISWTTSTG*EQRITSPPN--KIS*GLESKRLE 445
S L+ P + S++P+ +S +++ +QR +P N S ++SK+LE
Sbjct: 130 SPLLPPNGSESSSPIPLSLLSTSSLQQRKITPSNLSNTSKPMDSKQLE 177
>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1583
Score = 26.2 bits (55), Expect = 4.4
Identities = 17/56 (30%), Positives = 33/56 (58%), Gaps = 5/56 (8%)
Frame = -2
Query: 336 LVLVGTTKALLNARVGPEPFHRGEKLLTERFRVLHPLN-----HVEHHLTIAFSVS 184
L+++ T ++L A +GP +KL ERF++L +N +++ ++ AFS+S
Sbjct: 1469 LMIISTVDSVL-ATIGPTITGWMKKLDHERFKILAGINLCNLIYLKRYIKYAFSIS 1523
>SPAC869.10c |||proline specific permease |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 552
Score = 26.2 bits (55), Expect = 4.4
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = -2
Query: 609 FILVQHLIIFRHGNTEYDRGHVLEAMNPFL 520
FIL L+IF G TE R ++ +A + F+
Sbjct: 251 FILAPELVIFSAGETEAPRRNIPKATSRFI 280
>SPBC27B12.12c |||CorA family magnesium ion transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 803
Score = 25.8 bits (54), Expect = 5.8
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = -1
Query: 307 PERQSRPRAFSSRREAPH*TVPCPP 233
P R S ++ S+ + PH T+P PP
Sbjct: 275 PRRPSATKSCSAAVDCPHTTIPKPP 299
>SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 749
Score = 25.4 bits (53), Expect = 7.7
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = +2
Query: 11 VLANRARVL*SNR*KLSMKVDSQMKTSNSTGPWS 112
+LAN R++ SN K+ D Q + S GPW+
Sbjct: 1 MLANALRLV-SNSKKIKYDYDIQKENSVQVGPWT 33
>SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 374
Score = 25.4 bits (53), Expect = 7.7
Identities = 20/64 (31%), Positives = 30/64 (46%)
Frame = +2
Query: 317 VVPTSTNSTTPLNISWTTSTG*EQRITSPPNKIS*GLESKRLESSKYISPSKTSISNCST 496
V +S +STT + S + S+ TSP + S S SS S S +S S+ S+
Sbjct: 127 VSSSSVSSTTSSSSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS 186
Query: 497 WAGS 508
+ S
Sbjct: 187 SSSS 190
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,996,832
Number of Sequences: 5004
Number of extensions: 63695
Number of successful extensions: 234
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 210
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 232
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 313902888
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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