Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ceN-5186
         (623 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr 1|...    98   1e-21
SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr 3|||Ma...    39   5e-04
SPBP4H10.18c |||sequence orphan|Schizosaccharomyces pombe|chr 2|...    28   0.95 
SPAC17H9.11 |||cofilin/tropomyosin family protein|Schizosaccharo...    28   1.3  
SPAC29A4.10 |rrn5||RNA polymerase I upstream activation factor c...    27   2.2  
SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun...    27   2.2  
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi...    26   5.1  
SPAC4G9.17c |mrps5||mitochondrial ribosomal protein subunit S5|S...    25   6.7  
SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr...    25   6.7  
SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr 2||...    25   6.7  
SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual     25   8.9  
SPBP26C9.03c |||iron ion transporter |Schizosaccharomyces pombe|...    25   8.9  

>SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 137

 Score = 97.9 bits (233), Expect = 1e-21
 Identities = 54/140 (38%), Positives = 81/140 (57%)
 Frame = +3

Query: 87  SGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTG 266
           SGV VS  C   ++E+K  K  RYVVF + D K   V      + +++ FL DL +    
Sbjct: 4   SGVKVSPECLEAFQELKLGKSLRYVVFKMNDTKTEIVVEKKSTDKDFDTFLGDLPEK--- 60

Query: 267 ECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQ 446
           +CRY ++DFE+ +  +G      + K+  +SW PD A +K KM+YSSS D L+++  G+ 
Sbjct: 61  DCRYAIYDFEF-NLGEGV-----RNKIIFISWSPDVAPIKSKMVYSSSKDTLRRAFTGIG 114

Query: 447 KYIQATDLSEASQEAVEKKL 506
             IQATD SE + E V +K+
Sbjct: 115 TDIQATDFSEVAYETVLEKV 134


>SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 328

 Score = 39.1 bits (87), Expect = 5e-04
 Identities = 38/137 (27%), Positives = 62/137 (45%), Gaps = 2/137 (1%)
 Frame = +3

Query: 153 RYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTGECRYGLFDFEYTHQCQGTSEAS 332
           R  +  I +E   DV+T+ E++   E    D +K    EC  G  +  +        + S
Sbjct: 26  RAAIISISNENSFDVKTMVEKSESIES---DFKK--VRECLLGSEEPAFVL----VYDDS 76

Query: 333 KKQKLFLMSWCPDTAKVKKKMLYSSSFDALKK--SLVGVQKYIQATDLSEASQEAVEKKL 506
           KK  L L+S+ P+ A V++KMLY+SS  A  +  +L  + +   A+   E   + + K L
Sbjct: 77  KKNLLQLISYVPENANVRRKMLYASSRAAFVRCVTLAKLDESYFASTPEELDYQQIMKSL 136

Query: 507 RATDRQ*TAFTHELATK 557
              + Q      EL  K
Sbjct: 137 SKQEDQSPLRQDELERK 153



 Score = 28.7 bits (61), Expect = 0.72
 Identities = 16/56 (28%), Positives = 32/56 (57%), Gaps = 5/56 (8%)
 Frame = +3

Query: 342 KLFLMSWCPDTAKVKKKMLYSSS----FDALKKSL-VGVQKYIQATDLSEASQEAV 494
           K+  +  CP  A VK +M+YSSS     D++K  L + +   I++ D ++ +++ +
Sbjct: 244 KILFIYICPMQATVKHRMVYSSSKLGLLDSIKAELGIVIDGKIESNDAADITEKEI 299


>SPBP4H10.18c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 314

 Score = 28.3 bits (60), Expect = 0.95
 Identities = 35/163 (21%), Positives = 65/163 (39%), Gaps = 3/163 (1%)
 Frame = +3

Query: 60  LRE*HQKMASGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGE-RNAEYEQF 236
           LR    ++  G    D+  ++ +E  ++KK +   F  RD++++ ++     R       
Sbjct: 41  LRSEGSELIFGGRSFDSLLSSIKETMEEKKKKSSSFEKRDKRRVQLKEKSPLRTPRNRTQ 100

Query: 237 LEDLQ--KGGTGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSS 410
           + D +  +  T        +F+   Q   T  A+K     +    P T    K  + ++ 
Sbjct: 101 IIDARSTRKDTENESLRKNNFKRRTQFAFTGRATKSNAGVMDVQSPSTMSTSKNNVRNAE 160

Query: 411 FDALKKSLVGVQKYIQATDLSEASQEAVEKKLRATDRQ*TAFT 539
             A KK + G +KY    + S    +  E K R  DR  + FT
Sbjct: 161 RPASKKPVFGSKKYFDVINDSNVENKE-ETKDRNLDRALSKFT 202


>SPAC17H9.11 |||cofilin/tropomyosin family
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 141

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 16/63 (25%), Positives = 30/63 (47%)
 Frame = +3

Query: 318 TSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEASQEAVE 497
           T++      LF++ W P        M+Y+S+     + +  V K  +A D  + + EAV+
Sbjct: 79  TTDGRLSTPLFMIYWRPSATPNDLSMIYASA-KVWFQDVSQVHKVFEARDSEDITSEAVD 137

Query: 498 KKL 506
           + L
Sbjct: 138 EFL 140


>SPAC29A4.10 |rrn5||RNA polymerase I upstream activation factor
           complex subunit Rrn5|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 556

 Score = 27.1 bits (57), Expect = 2.2
 Identities = 22/87 (25%), Positives = 38/87 (43%), Gaps = 4/87 (4%)
 Frame = -2

Query: 562 FGFVASSCVNAVYWRSVARSFFSTAS*DASERSVAWMYF*TPTR---DFFRASNELEYNI 392
           +G ++ SCV+  YW +  +  F  A     +R +  + +  P++      R  N LE  +
Sbjct: 61  WGVLSGSCVDGTYWSAEEKELFFQAVARNGKRDLDLIAYSIPSKSAVQIERYINALENEL 120

Query: 391 FFLTLAVSGHHDIR-KSFCFLLASDVP 314
            +L      H D   +S C L   D+P
Sbjct: 121 RWL----RNHVDASVRSQCLLKYEDIP 143


>SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 214

 Score = 27.1 bits (57), Expect = 2.2
 Identities = 10/26 (38%), Positives = 19/26 (73%)
 Frame = +3

Query: 408 SFDALKKSLVGVQKYIQATDLSEASQ 485
           S +AL++ L G + Y+Q+TD++  S+
Sbjct: 188 SLEALQEELEGFEDYVQSTDIAAMSK 213


>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
           Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 690

 Score = 25.8 bits (54), Expect = 5.1
 Identities = 12/44 (27%), Positives = 23/44 (52%), Gaps = 5/44 (11%)
 Frame = -2

Query: 361 HDIRKSFCFLLASDVPWHWCVYSKSNRPY-----LHSPVPPFCR 245
           HD+     +++ S   +H C  ++S  P+     LH+P+P  C+
Sbjct: 107 HDVASHPSYMVQSPTSYHACSNNQSPFPHSHHPPLHNPLPVSCQ 150


>SPAC4G9.17c |mrps5||mitochondrial ribosomal protein subunit
           S5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 387

 Score = 25.4 bits (53), Expect = 6.7
 Identities = 11/23 (47%), Positives = 16/23 (69%)
 Frame = +2

Query: 20  CRCRGVGEVSGVIFT*ITSKNGV 88
           CRC G+ ++SG I   + SKNG+
Sbjct: 330 CRCAGIKDISGEI---LGSKNGM 349


>SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 319

 Score = 25.4 bits (53), Expect = 6.7
 Identities = 14/61 (22%), Positives = 31/61 (50%)
 Frame = +3

Query: 114 KTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTGECRYGLFDF 293
           K + + ++ + K ++     +D K+ D + +  +  EY  F ++L+   TG+  Y   DF
Sbjct: 108 KCSSDFLEANVKSQFTTCENKDSKE-DYQILHNKRLEYVSFNDELKSLETGQPLYCFQDF 166

Query: 294 E 296
           +
Sbjct: 167 Q 167


>SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 357

 Score = 25.4 bits (53), Expect = 6.7
 Identities = 13/46 (28%), Positives = 20/46 (43%), Gaps = 2/46 (4%)
 Frame = -2

Query: 313 WHWCVYSKSNRPY--LHSPVPPFCRSSRNCSYSALRSPTVSTSICF 182
           W++  Y+ S R Y      VPP+CR         +R+     + CF
Sbjct: 122 WNFVDYALSLRKYGWTDDVVPPYCRKVLTYKSLGIRTAVYVGAFCF 167


>SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 366

 Score = 25.0 bits (52), Expect = 8.9
 Identities = 10/22 (45%), Positives = 11/22 (50%)
 Frame = +3

Query: 96  TVSDACKTTYEEIKKDKKHRYV 161
           TV D C   +  IK   KH YV
Sbjct: 316 TVEDFCNNIHSSIKSQFKHAYV 337


>SPBP26C9.03c |||iron ion transporter |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 584

 Score = 25.0 bits (52), Expect = 8.9
 Identities = 14/35 (40%), Positives = 19/35 (54%)
 Frame = +3

Query: 93  VTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDV 197
           VT  D  KT  +  +K  KH+ V  YIR  K +D+
Sbjct: 113 VTNDDGTKTKVDSDEKKHKHKNVRDYIR-FKHVDI 146


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,307,183
Number of Sequences: 5004
Number of extensions: 44147
Number of successful extensions: 149
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 146
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 275671126
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)