Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ceN-5034
         (756 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyce...    29   0.95 
SPAC1039.03 |||esterase/lipase |Schizosaccharomyces pombe|chr 1|...    29   0.95 
SPBC725.15 |ura5||orotate phosphoribosyltransferase Ura5 |Schizo...    28   1.7  
SPCC550.03c |||RNA helicase involved in mRNA catabolism|Schizosa...    27   2.2  
SPBC1347.03 |meu14||sporulation protein Meu14|Schizosaccharomyce...    27   3.8  
SPAC23D3.13c |||guanyl-nucleotide exchange factor|Schizosaccharo...    26   5.0  
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce...    26   6.7  
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1...    26   6.7  
SPAC4A8.07c |||sphingoid long chain base |Schizosaccharomyces po...    26   6.7  

>SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 242

 Score = 28.7 bits (61), Expect = 0.95
 Identities = 19/76 (25%), Positives = 38/76 (50%), Gaps = 1/76 (1%)
 Frame = +3

Query: 207 LYLLAELKT-ISLKVTTVDMQKPPPDFRTNFEATHPPILIDNGLAILENEKIERHIMKSV 383
           +++L ELK    +KV      + PP +         PI++D+G+  +E+  I  H+++  
Sbjct: 16  VWMLEELKVPYEIKVYDRVDGRAPPAYTKLSPLGKSPIVVDDGVTYIESAAILEHLVRKY 75

Query: 384 PGGHNLFVQDKEVASL 431
             G +    +++VA L
Sbjct: 76  --GPSFKPSEEDVAEL 89


>SPAC1039.03 |||esterase/lipase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 341

 Score = 28.7 bits (61), Expect = 0.95
 Identities = 21/57 (36%), Positives = 25/57 (43%), Gaps = 3/57 (5%)
 Frame = -1

Query: 561 PLGTWSPCVLEVHR--CVRDELVTP-LISVHLCEPAPASVLNTGSRLETRPPCPARI 400
           P G W PC L  H    V   + T    + H+CE A   V+N   RL    P PA I
Sbjct: 96  PEGGW-PCFLWFHGGGWVLGNINTENSFATHMCEQAKCVVVNVDYRLAPEDPFPACI 151


>SPBC725.15 |ura5||orotate phosphoribosyltransferase Ura5
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 215

 Score = 27.9 bits (59), Expect = 1.7
 Identities = 10/25 (40%), Positives = 17/25 (68%)
 Frame = -2

Query: 752 VVVDDVLVGGTALRERVKLIHVIHV 678
           +++DDV+  GTA+RE +  +   HV
Sbjct: 121 LLLDDVITAGTAIREAISFLEPKHV 145


>SPCC550.03c |||RNA helicase involved in mRNA
           catabolism|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1213

 Score = 27.5 bits (58), Expect = 2.2
 Identities = 16/51 (31%), Positives = 24/51 (47%)
 Frame = +3

Query: 414 KEVASLIENLYSKLKLVLVRKDEQKSAALRAHLGRIDGLLERRETRFLTGD 566
           KEV  LIE+L SK     +RK   K+  +R     I  L +  +   + G+
Sbjct: 101 KEVPELIEDLNSKNSSSFLRKPSSKNEFVRGSTSNIPFLADDSDVDAIAGE 151


>SPBC1347.03 |meu14||sporulation protein Meu14|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 335

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 21/90 (23%), Positives = 40/90 (44%)
 Frame = +3

Query: 447 SKLKLVLVRKDEQKSAALRAHLGRIDGLLERRETRFLTGDTMCCFDCELMPRLQHIRVAG 626
           +++K   +R  E   A  RAH  R+   +ER E R      +     +L+ R +   + G
Sbjct: 114 NRIKFKAIRAMEDSIAPSRAHRQRLISSIEREEERDPLSPKLTDLQNQLV-RTEAENLVG 172

Query: 627 KYFVDFEIPTTFRALWRYMYHMYQLDAFTQ 716
           +  +D      F++ ++ +   +QL A  Q
Sbjct: 173 EMQLDNTSREVFKSSFQGLMDAFQLRAQKQ 202


>SPAC23D3.13c |||guanyl-nucleotide exchange
           factor|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1616

 Score = 26.2 bits (55), Expect = 5.0
 Identities = 13/39 (33%), Positives = 22/39 (56%)
 Frame = +3

Query: 351 EKIERHIMKSVPGGHNLFVQDKEVASLIENLYSKLKLVL 467
           +KIERHI  S+     L + D     ++E+L++ L  V+
Sbjct: 67  KKIERHIYISLNSIQLLAINDALSPDILESLFNSLNAVI 105


>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1610

 Score = 25.8 bits (54), Expect = 6.7
 Identities = 21/82 (25%), Positives = 35/82 (42%)
 Frame = +3

Query: 297 EATHPPILIDNGLAILENEKIERHIMKSVPGGHNLFVQDKEVASLIENLYSKLKLVLVRK 476
           E T   IL       L N+ ++ H++      H+L      +    ++LYS L ++L  K
Sbjct: 690 EETIKNILFTTLEYFLTNDWVDNHLIILCASLHSLKAHLPTIFDENKSLYSLLPVILFSK 749

Query: 477 DEQKSAALRAHLGRIDGLLERR 542
            E     + AH   I  LL+ +
Sbjct: 750 PED-DGHVAAHFESIFSLLKEK 770


>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 800

 Score = 25.8 bits (54), Expect = 6.7
 Identities = 20/56 (35%), Positives = 23/56 (41%), Gaps = 7/56 (12%)
 Frame = +1

Query: 607 STSASPGSTSWTSKYRRRSVLCGVTCITCISL-------TRSRKAVPPTRTSSTTT 753
           ST   P STS T            +C T  S+       T     VPPT TSST+T
Sbjct: 314 STPPPPASTSSTGTSSSPLPSTSTSCTTSTSIPPTGNSTTPVTPTVPPTSTSSTST 369



 Score = 25.4 bits (53), Expect = 8.8
 Identities = 25/85 (29%), Positives = 42/85 (49%), Gaps = 1/85 (1%)
 Frame = +1

Query: 505 LISDASMDF*NAGRPGS*RATLCAVSTAS*CRGCSTSASPGSTSWTSKYRRRSVLCGVT- 681
           +I++++    +A   GS  + L + ST+     C+TS S   T  +S     S+   +T 
Sbjct: 87  IITESTSSTSSASTTGSSSSPLPSTSTS-----CTTSTSIPPTGGSS-----SLSTPITP 136

Query: 682 CITCISLTRSRKAVPPTRTSSTTTN 756
            +   S + +   +PPT TSST TN
Sbjct: 137 TVPPTSTSSTSIPIPPTSTSSTDTN 161


>SPAC4A8.07c |||sphingoid long chain base |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 458

 Score = 25.8 bits (54), Expect = 6.7
 Identities = 14/44 (31%), Positives = 23/44 (52%), Gaps = 1/44 (2%)
 Frame = +3

Query: 405 VQDKEVASLIENLYSKLKLVLVR-KDEQKSAALRAHLGRIDGLL 533
           + + E   +  + +S  ++VL R KD  KS A    +G  DG+L
Sbjct: 124 IWESEAEPVFSSAHSICEVVLTRRKDHAKSIAKNLDVGSYDGIL 167


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,135,277
Number of Sequences: 5004
Number of extensions: 63920
Number of successful extensions: 205
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 193
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 204
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 361294920
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)