Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-4117
(564 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 150 1e-37
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 103 2e-23
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 98 9e-22
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 93 2e-20
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 87 2e-18
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 86 4e-18
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 71 2e-13
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 67 1e-12
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 67 2e-12
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 64 1e-11
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 61 1e-10
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 60 3e-10
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 54 1e-08
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 28 1.1
SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|ch... 27 1.9
SPAC31G5.15 |||phosphatidylserine decarboxylase |Schizosaccharom... 25 5.8
SPCC757.11c |||membrane transporter|Schizosaccharomyces pombe|ch... 25 5.8
SPBC215.07c |||PWWP domain protein|Schizosaccharomyces pombe|chr... 25 7.7
>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 148
Score = 150 bits (364), Expect = 1e-37
Identities = 69/117 (58%), Positives = 82/117 (70%)
Frame = +3
Query: 174 ALPRRIIKETQRLMQEPVPGISAVPSETNARYFHVIVTGPEDSXXXXXXXXXXXXXXXDY 353
ALP+RIIKE + L ++P PGI A P+E N RYF + + GP+ S +Y
Sbjct: 2 ALPKRIIKEIETLTRDPPPGIVAAPTEDNLRYFKITMEGPQQSAYEGGKFHLELFLPDEY 61
Query: 354 PMSAPKVRFITKIYHPNIDRLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDD 524
PM P VRF+TKIYHPN+D+LGRICL LK WSPALQIRTVLLSIQAL+ APNPDD
Sbjct: 62 PMMPPNVRFLTKIYHPNVDKLGRICLSTLKKDWSPALQIRTVLLSIQALMGAPNPDD 118
Score = 25.0 bits (52), Expect = 7.7
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = +1
Query: 526 PLANDVAELWKVN 564
PL NDVA++WK N
Sbjct: 119 PLDNDVAKIWKEN 131
>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 103 bits (246), Expect = 2e-23
Identities = 52/114 (45%), Positives = 66/114 (57%)
Frame = +3
Query: 183 RRIIKETQRLMQEPVPGISAVPSETNARYFHVIVTGPEDSXXXXXXXXXXXXXXXDYPMS 362
+RI +E L ++P SA P + ++ + GP DS DYP
Sbjct: 4 KRINRELADLGKDPPSSCSAGPVGDDLFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFK 63
Query: 363 APKVRFITKIYHPNIDRLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDD 524
PKV F T+IYHPNI+ G ICLDIL+D+WSPAL I VLLSI +LL+ PNPDD
Sbjct: 64 PPKVNFTTRIYHPNINSNGSICLDILRDQWSPALTISKVLLSICSLLTDPNPDD 117
>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 176
Score = 97.9 bits (233), Expect = 9e-22
Identities = 46/115 (40%), Positives = 67/115 (58%), Gaps = 1/115 (0%)
Frame = +3
Query: 174 ALPRRIIKETQRLMQEPVPGISAVP-SETNARYFHVIVTGPEDSXXXXXXXXXXXXXXXD 350
++ +R+ E LM PGISA P S++N ++ +TGP D+ +
Sbjct: 28 SVTKRLRSELMSLMMSNTPGISAFPDSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPAN 87
Query: 351 YPMSAPKVRFITKIYHPNIDRLGRICLDILKDKWSPALQIRTVLLSIQALLSAPN 515
YP S P + F + ++HPN+D G ICLDILKDKWS ++T+LLS+Q+LL PN
Sbjct: 88 YPYSPPTIIFTSPMWHPNVDMSGNICLDILKDKWSAVYNVQTILLSLQSLLGEPN 142
>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 151
Score = 93.5 bits (222), Expect = 2e-20
Identities = 38/111 (34%), Positives = 65/111 (58%)
Frame = +3
Query: 183 RRIIKETQRLMQEPVPGISAVPSETNARYFHVIVTGPEDSXXXXXXXXXXXXXXXDYPMS 362
RR++++ +R+ Q+P G+SA P N ++ ++ GP D+ YP
Sbjct: 7 RRLMRDFKRMQQDPPAGVSASPVSDNVMLWNAVIIGPADTPFEDGTFKLVLSFDEQYPNK 66
Query: 363 APKVRFITKIYHPNIDRLGRICLDILKDKWSPALQIRTVLLSIQALLSAPN 515
P V+F++ ++HPN+ G +CLDIL+++WSP + +L SIQ+LL+ PN
Sbjct: 67 PPLVKFVSTMFHPNVYANGELCLDILQNRWSPTYDVAAILTSIQSLLNDPN 117
>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 217
Score = 86.6 bits (205), Expect = 2e-18
Identities = 43/115 (37%), Positives = 60/115 (52%), Gaps = 1/115 (0%)
Frame = +3
Query: 183 RRIIKETQRLMQEPVPGISAVPSETNARYFHVIVTGPEDSXXXXXXXXXXXXXXXDYPMS 362
RRI KE + Q+ GI + + + GPE + DYP
Sbjct: 7 RRIAKELADVQQDKQAGIQVWTINDDISHLKGMFRGPEGTPYEGGYFVVDIEIPIDYPFR 66
Query: 363 APKVRFITKIYHPNID-RLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDD 524
PK+ F TKIYHPN+ + G ICLDILKD+WSP +++ L+S+Q+LL P P +
Sbjct: 67 PPKMNFDTKIYHPNVSSQTGAICLDILKDQWSPVYTMKSALISLQSLLCTPEPSN 121
>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
Length = 155
Score = 85.8 bits (203), Expect = 4e-18
Identities = 43/115 (37%), Positives = 59/115 (51%), Gaps = 1/115 (0%)
Frame = +3
Query: 183 RRIIKETQRLMQEPVPGISAVPSETNARYFHVIVTGPEDSXXXXXXXXXXXXXXXDYPMS 362
RR+ KE L + P+P I + N ++ GP DS DYP
Sbjct: 10 RRLTKEYSDLREHPIPDIRVNLVDDNLFHWACTALGPSDSVYAGGKFHFSLKFPLDYPFQ 69
Query: 363 APKVRFITKIYHPNIDRLGRICLDILKDK-WSPALQIRTVLLSIQALLSAPNPDD 524
P + F T+IYHPN D G +CL ILK + + P++++R+VL I LL PNPDD
Sbjct: 70 PPTIEFTTRIYHPNFDSEGNVCLAILKQQVFKPSIKLRSVLEQILQLLREPNPDD 124
>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 177
Score = 70.5 bits (165), Expect = 2e-13
Identities = 27/59 (45%), Positives = 41/59 (69%)
Frame = +3
Query: 348 DYPMSAPKVRFITKIYHPNIDRLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDD 524
+YP PKV+ + KIYHPNID G +CL+IL+ W+P L + ++L+ +Q L +PN +D
Sbjct: 79 NYPHDPPKVKCLNKIYHPNIDIEGNVCLNILRQDWNPVLNLNSILVGLQFLFLSPNAED 137
>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 67.3 bits (157), Expect = 1e-12
Identities = 35/137 (25%), Positives = 67/137 (48%), Gaps = 14/137 (10%)
Frame = +3
Query: 183 RRIIKETQRLMQEPVPGISAVPSETNARY-FHVIVTGPEDSXXXXXXXXXXXXXXXDYPM 359
RR++KE + L + GI+A PS + + + ++ GP+ + DYP+
Sbjct: 8 RRLMKEYKELTENGPDGITAGPSNEDDFFTWDCLIQGPDGTPFEGGLYPATLKFPSDYPL 67
Query: 360 SAPKVRFITKIYHPNIDRLGRICLDIL-------------KDKWSPALQIRTVLLSIQAL 500
P ++F + +HPN+ + G +C+ IL ++WSP + +LLS+ ++
Sbjct: 68 GPPTLKFECEFFHPNVYKDGTVCISILHAPGDDPNMYESSSERWSPVQSVEKILLSVMSM 127
Query: 501 LSAPNPDDSAGERRCRI 551
L+ PN + A C++
Sbjct: 128 LAEPNDESGANIDACKM 144
>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 160
Score = 66.9 bits (156), Expect = 2e-12
Identities = 37/111 (33%), Positives = 55/111 (49%), Gaps = 3/111 (2%)
Frame = +3
Query: 183 RRIIKETQRLMQEPVPGISAVPSETNARYFH--VIVTGPEDSXXXXXXXXXXXXXXXDYP 356
+R+ KE + G+ + T+ FH ++ GP ++ YP
Sbjct: 6 KRLHKEYASFQAGDISGLLLLKPVTDVDMFHWKAVIEGPTETPYEGGQWVLDIHVHEGYP 65
Query: 357 MSAPKVRFITKIYHPNIDRL-GRICLDILKDKWSPALQIRTVLLSIQALLS 506
+S P V F TKI HPNI G +C+DILK WSPA +++ L+I +LLS
Sbjct: 66 ISPPSVYFQTKIVHPNISWTNGEVCMDILKTHWSPAWSLQSACLAIISLLS 116
>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
Length = 167
Score = 64.1 bits (149), Expect = 1e-11
Identities = 34/126 (26%), Positives = 62/126 (49%), Gaps = 14/126 (11%)
Frame = +3
Query: 195 KETQRLMQEPVPGISA-VPSETNARYFHVIVTGPEDSXXXXXXXXXXXXXXXDYPMSAPK 371
K+ + + + P G S + + + + V++ GPED+ DYP+ PK
Sbjct: 12 KQLKEIQKNPPQGFSVGLVDDKSIFEWEVMIIGPEDTLYEGGFFHATLSFPQDYPLMPPK 71
Query: 372 VRFITKIYHPNIDRLGRICLDILK-------------DKWSPALQIRTVLLSIQALLSAP 512
++F T+I+HPN+ G +C+ IL ++W P T+L+S+ ++LS+P
Sbjct: 72 MKFTTEIWHPNVHPNGEVCISILHPPGDDKYGYEDAGERWLPVHSPETILISVISMLSSP 131
Query: 513 NPDDSA 530
N + A
Sbjct: 132 NDESPA 137
>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 61.3 bits (142), Expect = 1e-10
Identities = 34/121 (28%), Positives = 58/121 (47%), Gaps = 2/121 (1%)
Frame = +3
Query: 168 MAALPRRIIKETQRLMQEPVPGISAVPSETNARYFHVIVTGPEDSXXXXXXXXXXXXXXX 347
M++ RRI + +L+ ++ V N + F+V GP ++
Sbjct: 1 MSSPRRRIETDVMKLLMSDYE-VTLV--NDNMQEFYVRFHGPSETPYSGGIWKVHVELPS 57
Query: 348 DYPMSAPKVRFITKIYHPNIDRL-GRICLDILKDKWSPALQIRTVL-LSIQALLSAPNPD 521
+YP +P + F+ +I+HPNID L G +CLD++ WSP + + + + LL PN
Sbjct: 58 EYPWKSPSIGFVNRIFHPNIDELSGSVCLDVINQTWSPMFDMINIFEVFLPQLLRYPNAS 117
Query: 522 D 524
D
Sbjct: 118 D 118
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 59.7 bits (138), Expect = 3e-10
Identities = 26/58 (44%), Positives = 36/58 (62%), Gaps = 2/58 (3%)
Frame = +3
Query: 348 DYPMSAPKVRFITKIYHPNIDRLGRICLDILKDK--WSPALQIRTVLLSIQALLSAPN 515
+YP PK RF ++HPN+ G +CL IL ++ W PA+ I+ +LL IQ LL PN
Sbjct: 67 EYPTRPPKCRFTPPLFHPNVYPSGTVCLSILNEEEGWKPAITIKQILLGIQDLLDDPN 124
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 54.4 bits (125), Expect = 1e-08
Identities = 30/112 (26%), Positives = 51/112 (45%), Gaps = 3/112 (2%)
Frame = +3
Query: 183 RRIIKETQRLMQEPVPGISAVPSETNARYFHVIVTGPEDSXXXXXXXXXXXXXXXDYPMS 362
+R++KE L + PV + A P+ N +H I+TGP D+ DYP
Sbjct: 8 KRLMKEYLALQKNPVELVDAKPATENILEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFK 67
Query: 363 APKVRFITKIYHPNIDRLGRICL---DILKDKWSPALQIRTVLLSIQALLSA 509
P +R IT R+CL D W+P+ + T+L+ + + +++
Sbjct: 68 PPAIRMITP--SGRFQTNTRLCLSFSDFHPKSWNPSWMVSTILVGLVSFMTS 117
>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
Mms2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 27.9 bits (59), Expect = 1.1
Identities = 19/79 (24%), Positives = 35/79 (44%), Gaps = 4/79 (5%)
Frame = +3
Query: 270 FHVIVTGPEDSXXXXXXXXXXXXXXXDYPMSAPKVRFITKIYHPNID-RLGRI---CLDI 437
++ + GP S +YP + P V F+++I P +D G++ +D
Sbjct: 41 WNATILGPAHSVHENRIYSLKIHCDANYPDAPPIVTFVSRINLPGVDGETGKVNPHKIDC 100
Query: 438 LKDKWSPALQIRTVLLSIQ 494
L+ W + TVLL ++
Sbjct: 101 LR-HWKREYSMETVLLDLK 118
>SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|chr
3|||Manual
Length = 923
Score = 27.1 bits (57), Expect = 1.9
Identities = 9/26 (34%), Positives = 16/26 (61%)
Frame = +3
Query: 375 RFITKIYHPNIDRLGRICLDILKDKW 452
+FI ++HP+ RL R+C + + W
Sbjct: 219 KFILSVFHPDAARLYRLCSSLKRICW 244
>SPAC31G5.15 |||phosphatidylserine decarboxylase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 980
Score = 25.4 bits (53), Expect = 5.8
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = +3
Query: 423 ICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDS 527
+CL K KW + + + IQALL + NPD++
Sbjct: 111 LCL-YRKSKWKKQI-VGEAYIGIQALLLSTNPDET 143
>SPCC757.11c |||membrane transporter|Schizosaccharomyces pombe|chr
3|||Manual
Length = 471
Score = 25.4 bits (53), Expect = 5.8
Identities = 8/26 (30%), Positives = 15/26 (57%)
Frame = +3
Query: 360 SAPKVRFITKIYHPNIDRLGRICLDI 437
++P V +TK+ HP +G + + I
Sbjct: 393 NSPSVSLLTKVLHPKYHLIGNVAVQI 418
>SPBC215.07c |||PWWP domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 568
Score = 25.0 bits (52), Expect = 7.7
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = +3
Query: 402 NIDRLGRICLDILKDKWSPALQ 467
N D + IC DIL+++W LQ
Sbjct: 416 NEDEVKSICGDILENRWKSLLQ 437
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,201,781
Number of Sequences: 5004
Number of extensions: 42184
Number of successful extensions: 99
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 88
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 92
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 238029836
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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