BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-3982
(721 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23H4.12 |alp13||Clr6 histone deacetylase complex subunit Alp... 55 1e-08
SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosacc... 28 1.5
SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|ch... 27 3.6
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 26 4.7
SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces ... 26 6.2
SPAC2E12.03c |||G-protein coupled receptor |Schizosaccharomyces ... 26 6.2
SPAC19A8.07c |||U3 snoRNP-associated protein Imp4 |Schizosacchar... 25 8.2
SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizos... 25 8.2
>SPAC23H4.12 |alp13||Clr6 histone deacetylase complex subunit
Alp13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 337
Score = 54.8 bits (126), Expect = 1e-08
Identities = 28/72 (38%), Positives = 46/72 (63%)
Frame = +2
Query: 272 ERVLCYEPDPTKAKVLYDSKVLEVVESKDKRGRRTVEYLIHFQGWNSSWDRCVSEDFVLK 451
ERVLC+ +LY++K+++ + +G T YLIH++GW +SWD V +D +L+
Sbjct: 9 ERVLCFH-----GPLLYEAKIVDT----EMKGDVTT-YLIHYKGWKNSWDEWVEQDRILQ 58
Query: 452 DTEENRQLQRDL 487
TEEN + Q++L
Sbjct: 59 WTEENLKTQKEL 70
>SPAC10F6.02c |prp22||ATP-dependent RNA helicase
Prp22|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1168
Score = 27.9 bits (59), Expect = 1.5
Identities = 13/37 (35%), Positives = 18/37 (48%)
Frame = -3
Query: 644 RLRLAGYLSKSRRR*SHDGCAWRAPPRRRLYYHPSFV 534
R +GY + +R SH+GC +Y HPS V
Sbjct: 1051 RALCSGYFTNVAKRDSHEGCYKTIVENAPVYMHPSGV 1087
>SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 778
Score = 26.6 bits (56), Expect = 3.6
Identities = 13/47 (27%), Positives = 22/47 (46%)
Frame = -2
Query: 300 GSGS*HRTRSPSVNLYRSPRVETIVN*CLAQNRWELNKITPFNKCDG 160
G+G + P N Y +P + + + + L ++TPF K DG
Sbjct: 525 GAGLPSKGYDPGSNTYVAPSSVNVKDVAIDPHSKRLQRLTPFKKWDG 571
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 26.2 bits (55), Expect = 4.7
Identities = 12/35 (34%), Positives = 18/35 (51%)
Frame = +2
Query: 20 NNSTVPLILLLHTTTWSHSDVRNQLISLGYYRSLF 124
++ST P L +W HS + L +L + R LF
Sbjct: 155 SSSTDPAFELKEDQSWKHSSILRPLETLNFKRFLF 189
>SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1502
Score = 25.8 bits (54), Expect = 6.2
Identities = 20/73 (27%), Positives = 30/73 (41%), Gaps = 5/73 (6%)
Frame = +2
Query: 149 LQI*PSHLLNGVILLSSHRF---WARHQLTMVSTRGLRYKFTEGERVLCYEPD--PTKAK 313
L + PS + LL S + W RH + + L K T E V ++PD K
Sbjct: 140 LLVCPSEDIKNDFLLDSLKHALHWDRHAKVVCTAIQLLVKVTGAEAVFAFQPDFFEQSLK 199
Query: 314 VLYDSKVLEVVES 352
+L D + + S
Sbjct: 200 LLKDYSYAQAISS 212
>SPAC2E12.03c |||G-protein coupled receptor |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 283
Score = 25.8 bits (54), Expect = 6.2
Identities = 14/41 (34%), Positives = 25/41 (60%), Gaps = 3/41 (7%)
Frame = +3
Query: 273 NESCATSQTLLKPRCF---MIPRFLKS*RAKTNEGVELLSI 386
N + +T +L C+ +IP+ +K+ RAK+ EG++ L I
Sbjct: 15 NATASTVFAILGTVCWCVQLIPQIIKNYRAKSTEGLDTLFI 55
>SPAC19A8.07c |||U3 snoRNP-associated protein Imp4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 289
Score = 25.4 bits (53), Expect = 8.2
Identities = 14/30 (46%), Positives = 17/30 (56%)
Frame = +2
Query: 449 KDTEENRQLQRDLAEKSQLQLGAYLYRRER 538
K E N++L +DL E SQLQ Y Y R
Sbjct: 34 KALEGNKELNKDLQEDSQLQKD-YKYDESR 62
>SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 647
Score = 25.4 bits (53), Expect = 8.2
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = -1
Query: 415 RRVPSLEMD*ILNSSTPSFVFALYDFKNLGIIKHLGFSRVWL 290
+R+PS + IL FV + +N G K +G +VWL
Sbjct: 559 QRLPSSDCSKILKCLLDGFVRNVAHLQNDGSYKTIGGKQVWL 600
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,729,108
Number of Sequences: 5004
Number of extensions: 53453
Number of successful extensions: 156
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 150
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 156
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 337208592
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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