BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-3758
(444 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 29 0.24
SPAC4G9.20c |||mitochondrial carrier with solute carrier repeats... 25 4.0
SPAC6F6.10c |arc2|arc34|ARP2/3 actin-organizing complex subunit ... 25 4.0
SPAC110.02 |pds5||cohesin-associated protein Pds5|Schizosaccharo... 25 4.0
SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizos... 25 5.2
SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyce... 25 6.9
SPBC2D10.05 |exg3||glucan 1,3-beta-glucosidase Exg3|Schizosaccha... 24 9.1
SPAC3G6.09c |tps2||trehalose-phosphate synthase Tps2 |Schizosacc... 24 9.1
SPAC11G7.06c |mug132||S. pombe specific UPF0300 family protein 3... 24 9.1
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 29.5 bits (63), Expect = 0.24
Identities = 16/47 (34%), Positives = 25/47 (53%)
Frame = +1
Query: 172 DQSLSFIELRDSIVLQWKCY*EKSSSQNYFALT*LLDNTKSILKYID 312
D IEL + IVLQ + E ++ QN LT + + +++YID
Sbjct: 995 DLPSQLIELLEKIVLQPSSFSENANLQNLLFLTAIKADKSRVMEYID 1041
>SPAC4G9.20c |||mitochondrial carrier with solute carrier
repeats|Schizosaccharomyces pombe|chr 1|||Manual
Length = 302
Score = 25.4 bits (53), Expect = 4.0
Identities = 9/26 (34%), Positives = 17/26 (65%)
Frame = +3
Query: 291 IDIKIYRLRNPLTHGPWFCMEYLKTK 368
I ++I +N L HGPW C++ + ++
Sbjct: 138 IRLQIQTGKNVLYHGPWDCIKKISSQ 163
>SPAC6F6.10c |arc2|arc34|ARP2/3 actin-organizing complex subunit
Arc34|Schizosaccharomyces pombe|chr 1|||Manual
Length = 317
Score = 25.4 bits (53), Expect = 4.0
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = -2
Query: 161 HYNIKANC*YMHSRMTIELMHYYIKLNLA 75
H++IKA+ YMH RM + + LN A
Sbjct: 270 HFHIKASKAYMHQRMRKRVADFQKVLNRA 298
>SPAC110.02 |pds5||cohesin-associated protein
Pds5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1205
Score = 25.4 bits (53), Expect = 4.0
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +3
Query: 21 IVNVVHRLFIDAMKKTDTCQVEFYVI 98
+VN+ RLF D +K T VEFY++
Sbjct: 144 LVNIF-RLFFDLARKGTTKNVEFYML 168
>SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 556
Score = 25.0 bits (52), Expect = 5.2
Identities = 15/43 (34%), Positives = 19/43 (44%)
Frame = -2
Query: 134 YMHSRMTIELMHYYIKLNLACIRFLHCIYK*SMYNIYNTNKLL 6
Y HS T+ L YY L+ F IYK + Y N + L
Sbjct: 203 YFHSDNTVLLNEYYKSLSKLKNNFRGIIYKTTPYGSCNDTESL 245
>SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 381
Score = 24.6 bits (51), Expect = 6.9
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = -2
Query: 374 LIFSFQIFHTKPWT 333
++ SFQ+ TKPWT
Sbjct: 104 IVLSFQVKPTKPWT 117
>SPBC2D10.05 |exg3||glucan 1,3-beta-glucosidase
Exg3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 464
Score = 24.2 bits (50), Expect = 9.1
Identities = 9/20 (45%), Positives = 12/20 (60%)
Frame = +3
Query: 294 DIKIYRLRNPLTHGPWFCME 353
D+ IYR + + G WFC E
Sbjct: 7 DLYIYRKQYGVNLGAWFCAE 26
>SPAC3G6.09c |tps2||trehalose-phosphate synthase Tps2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 849
Score = 24.2 bits (50), Expect = 9.1
Identities = 14/52 (26%), Positives = 26/52 (50%)
Frame = +2
Query: 5 IIIYLYCKCCTSIIYRCNEENGYMPS*VLCNSA*VRLSFLNAYTSSLL*YCN 160
I+ +L+ CTS ++RC + + VL ++ V ++YT L C+
Sbjct: 244 IVFFLHAPFCTSEVFRCLSKRAEILKGVLASN--VIAMQTDSYTRHFLSTCS 293
>SPAC11G7.06c |mug132||S. pombe specific UPF0300 family protein
3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 24.2 bits (50), Expect = 9.1
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = +3
Query: 312 LRNPLTHGPWFCMEYLKTKNQ*ARLGV*D 398
+RNP THG FC+ + ++GV D
Sbjct: 97 VRNPFTHG-LFCVVFYHQNKSYLKMGVTD 124
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,894,327
Number of Sequences: 5004
Number of extensions: 37672
Number of successful extensions: 81
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 69
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 81
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 162176800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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