Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ceN-3676
         (728 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC16H5.13 |||WD repeat protein |Schizosaccharomyces pombe|chr ...    29   0.51 
SPAC227.12 |||U4/U6 x U5 tri-snRNP complex subunit Prp4 family|S...    27   2.7  
SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces...    27   2.7  
SPAC19B12.02c |||1,3-beta-glucanosyltransferase|Schizosaccharomy...    26   6.3  
SPBC725.06c |ppk31|mug25|serine/threonine protein kinase Ppk31 |...    26   6.3  

>SPBC16H5.13 |||WD repeat protein |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 1026

 Score = 29.5 bits (63), Expect = 0.51
 Identities = 37/127 (29%), Positives = 54/127 (42%), Gaps = 13/127 (10%)
 Frame = -3

Query: 537 VIDLKKFTFLSLKYLITIFGDLGILLSDR*L*MSKTNLFSIKRFFKL*STIALCEF*TKS 358
           VID+     +S  Y + ++ D  +LL      ++  +L  I   F L    A C F   S
Sbjct: 203 VIDVVPADCISSNYALYVYSDAVVLLESHEKFIASYSLAEITSVFVLADGTA-CIFTLSS 261

Query: 357 E---RSSRTVEPSLIMISK*----PSKSASRIECAT*SLLPYDHK------TKTQPISCN 217
               +  ++ EP   +ISK     P KS + ++    SL  Y  K      T+    SCN
Sbjct: 262 STLLKLHQSPEPHFELISKYSGDFPWKSCTILKSKPVSLCVYPEKITFNWLTEGTVDSCN 321

Query: 216 SCKLSYT 196
            CKLS T
Sbjct: 322 LCKLSNT 328


>SPAC227.12 |||U4/U6 x U5 tri-snRNP complex subunit Prp4
           family|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 462

 Score = 27.1 bits (57), Expect = 2.7
 Identities = 19/77 (24%), Positives = 33/77 (42%)
 Frame = +2

Query: 263 RDHVAHSMRDADLEGYFDIIISEGSTVREERSDLVQNSHNAIVDYNLKKRFIENKFVFDI 442
           R  +   M +  LEG   + + +G++   E+   VQ SH  +V       +   K    +
Sbjct: 75  RQRLQQLMIEKSLEGDNPLDVDQGASENIEKETYVQGSHELLVARKKIALYSLEKAKLRL 134

Query: 443 HNHRSDNNIPKSPKIVI 493
              R  + IP  P+IV+
Sbjct: 135 KKEREISEIP-VPEIVL 150


>SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 486

 Score = 27.1 bits (57), Expect = 2.7
 Identities = 25/72 (34%), Positives = 34/72 (47%), Gaps = 4/72 (5%)
 Frame = +2

Query: 2   IRHEGVLSNKPPMYSFLKEWFLLP---HYKVVSLKS-ESLTWGFPHVVVFDLDSTLITEE 169
           I H+  LS+ PP  S L E  LLP   H  + S  S E+    F H+   DL    ++ E
Sbjct: 109 ICHDDCLSSSPPCTSALTEITLLPPTFHNLIPSSSSYETAVAEFLHME--DLLQENVSRE 166

Query: 170 EQIQIRDSFVYD 205
            + QI    +YD
Sbjct: 167 LECQICFGMLYD 178


>SPAC19B12.02c |||1,3-beta-glucanosyltransferase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 542

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 10/27 (37%), Positives = 16/27 (59%)
 Frame = +2

Query: 602 PTPVRDWHNYHEQIIDNLIEYDNMYNF 682
           PT   D  + + +++D+L  YDNM  F
Sbjct: 126 PTWTVDLFSRYTEVVDSLAPYDNMLGF 152


>SPBC725.06c |ppk31|mug25|serine/threonine protein kinase Ppk31
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1032

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 18/61 (29%), Positives = 30/61 (49%), Gaps = 1/61 (1%)
 Frame = +2

Query: 488 VIKYLSDKNVNFFKSITLVDDLPTNNYAY-DFYVKVKRCPTPVRDWHNYHEQIIDNLIEY 664
           V +YLS++   FF  +  + ++  NN  Y D   + +R  T  R  H  + +I + L E 
Sbjct: 397 VNQYLSEQRPVFFNRLLALGNVENNNSIYDDIQKRTERISTIKR--HKKYFEIGERLTEK 454

Query: 665 D 667
           D
Sbjct: 455 D 455


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,960,222
Number of Sequences: 5004
Number of extensions: 64174
Number of successful extensions: 206
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 203
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 206
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 343230174
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)