Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-3665
(660 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces pomb... 123 2e-29
SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme Fub2|Schiz... 72 6e-14
SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces ... 68 1e-12
SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme ... 52 1e-07
SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces ... 52 1e-07
SPBC6B1.05c |||ubiquitin-like conjugating enzyme|Schizosaccharom... 48 1e-06
SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces ... 40 2e-04
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 28 1.0
SPAC23C4.14 |alg1||mannosyltransferase complex subunit Alg1 |Sch... 27 1.8
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 27 3.2
SPBC3F6.03 |trr1|caf4|thioredoxin reductase Trr1|Schizosaccharom... 26 4.2
SPBC19C2.05 |ran1|pat1|serine/threonine protein kinase Ran1|Schi... 25 7.3
SPCC23B6.01c |||oxysterol binding protein |Schizosaccharomyces p... 25 9.7
SPAC20G8.04c |||mitochondrial electron transfer flavoprotein-ubi... 25 9.7
SPAC30C2.05 |erv14||cornichon family protein Erv14|Schizosacchar... 25 9.7
>SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 401
Score = 123 bits (297), Expect = 2e-29
Identities = 57/120 (47%), Positives = 78/120 (65%)
Frame = +1
Query: 301 RYSRQILLSDIGVEGQVKICSAKVLIVGAGGLGCPAAMYLAGAGIGEIGIVDYDAVDLTN 480
RY RQ+LLS+IG+ GQ+ + + VL++GAGGLGCPA YL AGIG +GI+D D VD +N
Sbjct: 23 RYGRQMLLSEIGLPGQLSLKRSSVLVIGAGGLGCPAMQYLVAAGIGTLGIMDGDVVDKSN 82
Query: 481 VHRQLLHHESNENTSKAFSAAESLRCINSKIKITPYNVQLDSKNAQQIASAYDLVLDCSD 660
+HRQ++H S + KA SA + L +N + I Y + N I YD+VLDC+D
Sbjct: 83 LHRQIIHSTSKQGMHKAISAKQFLEDLNPNVIINTYLEFASASNLFSIIEQYDVVLDCTD 142
>SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme
Fub2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 628
Score = 72.1 bits (169), Expect = 6e-14
Identities = 40/110 (36%), Positives = 60/110 (54%), Gaps = 2/110 (1%)
Frame = +1
Query: 337 VEGQVKICSAKVLIVGAGGLGCPAAMYLAGAGIGEIGIVDYDAVDLTNVHRQLLHHESNE 516
VE SAKVL+VGAGG+GC L +G+ E+ I+D D +DL+N++RQ L + +
Sbjct: 17 VEALRNFKSAKVLLVGAGGIGCELLKNLLMSGVKEVHIIDLDTIDLSNLNRQFLFRKKHV 76
Query: 517 NTSKAFSAAESLRCINSKIKITPY--NVQLDSKNAQQIASAYDLVLDCSD 660
KA AA++ N +K+ Y N++ D N +DLV + D
Sbjct: 77 KQPKAIVAAKTASSFNPNVKLEAYHANIKEDRFNVAWFRQ-FDLVFNALD 125
>SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 444
Score = 67.7 bits (158), Expect = 1e-12
Identities = 32/86 (37%), Positives = 52/86 (60%)
Frame = +1
Query: 361 SAKVLIVGAGGLGCPAAMYLAGAGIGEIGIVDYDAVDLTNVHRQLLHHESNENTSKAFSA 540
S+K+LI+GAGGLGC LA +G ++ ++D D +D+TN++RQ L +ESN + KA A
Sbjct: 44 SSKILIIGAGGLGCEILKDLALSGFRDLSVIDMDTIDITNLNRQFLFNESNIDEPKANVA 103
Query: 541 AESLRCINSKIKITPYNVQLDSKNAQ 618
A + +TP+ ++ K +
Sbjct: 104 ASMIMKRIPSTVVTPFYGKIQDKTIE 129
>SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1012
Score = 51.6 bits (118), Expect = 1e-07
Identities = 37/115 (32%), Positives = 57/115 (49%), Gaps = 7/115 (6%)
Frame = +1
Query: 265 SVDGCLPKWAIERYSRQILLSDIGVEGQVKICSAKVLIVGAGGLGCP-----AAMYLAGA 429
S + C P+ RY QI + G E Q KI S +VGAG +GC A M +A
Sbjct: 399 SEETCKPRGC--RYDGQIAV--FGSEFQEKIASLSTFLVGAGAIGCEMLKNWAMMGVATG 454
Query: 430 GIGEIGIVDYDAVDLTNVHRQLLHHESNENTSKAFSAAESLRCINSKI--KITPY 588
G I + D D+++ +N++RQ L + K+ A+ ++ +N + KIT Y
Sbjct: 455 ESGHISVTDMDSIEKSNLNRQFLFRPRDVGKLKSECASTAVSIMNPSLTGKITSY 509
Score = 35.1 bits (77), Expect = 0.009
Identities = 22/93 (23%), Positives = 42/93 (45%)
Frame = +1
Query: 304 YSRQILLSDIGVEGQVKICSAKVLIVGAGGLGCPAAMYLAGAGIGEIGIVDYDAVDLTNV 483
YSRQ+ + +G E ++ + VLI+G GLG A + AG+ + + D + ++
Sbjct: 20 YSRQLYV--LGHEAMKQMSQSNVLIIGCKGLGVEIAKNVCLAGVKSVTLYDPQPTRIEDL 77
Query: 484 HRQLLHHESNENTSKAFSAAESLRCINSKIKIT 582
Q E + +A L +N + ++
Sbjct: 78 SSQYFLTEDDIGVPRAKVTVSKLAELNQYVPVS 110
>SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 485
Score = 51.6 bits (118), Expect = 1e-07
Identities = 29/110 (26%), Positives = 58/110 (52%), Gaps = 1/110 (0%)
Frame = +1
Query: 334 GVEGQVKICSAKVLIVGAGGLGCPAAMYLAGAGIGEIGIVDYDAVDLTNVHRQLLHHESN 513
G +G ++ ++ V++VG GG+G LA +G+ +I IVD+D V L++++R + +
Sbjct: 117 GEDGMERLRNSFVIVVGCGGVGSWVINMLARSGVQKIRIVDFDQVSLSSLNRHSIATLQD 176
Query: 514 ENTSKAFSAAESLRCINSKIKITPYNVQLDSKNAQQIASAY-DLVLDCSD 660
T K + ++++ I++ N + +A + S D V+D D
Sbjct: 177 VGTPKTLAIKKAIKKFAPWIEVDARNALFNPDSADDLLSGNPDFVIDAID 226
>SPBC6B1.05c |||ubiquitin-like conjugating
enzyme|Schizosaccharomyces pombe|chr 2|||Manual
Length = 649
Score = 48.0 bits (109), Expect = 1e-06
Identities = 29/85 (34%), Positives = 43/85 (50%), Gaps = 2/85 (2%)
Frame = +1
Query: 352 KICSAKVLIVGAGGLGCPAAMYLAGAGIGEIGIVDYDAVDLTNVHRQLL--HHESNENTS 525
+I ++K L++GAG LGC A L G+ + VDY V +N RQ L +
Sbjct: 332 RIQNSKCLLLGAGTLGCGVARNLLSWGVRHVTFVDYSTVSYSNPVRQSLFTFEDCKRKLP 391
Query: 526 KAFSAAESLRCINSKIKITPYNVQL 600
KA AA+ L+ I + T YN+ +
Sbjct: 392 KAECAAQRLKEIYPNMFSTGYNISI 416
>SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 500
Score = 40.3 bits (90), Expect = 2e-04
Identities = 27/95 (28%), Positives = 44/95 (46%)
Frame = +1
Query: 295 IERYSRQILLSDIGVEGQVKICSAKVLIVGAGGLGCPAAMYLAGAGIGEIGIVDYDAVDL 474
+++Y RQ+ L EGQ I + V ++ A +GC A L GIG +VD +VD
Sbjct: 7 MQKYDRQVRLWK--AEGQNAIEKSHVCLLYANTVGCEALKNLILPGIGSFAVVDDTSVDF 64
Query: 475 TNVHRQLLHHESNENTSKAFSAAESLRCINSKIKI 579
+ E S+A A L+ +N +++
Sbjct: 65 SMDGMNFFIQYDQEGKSRARCTASLLQQLNPNVEM 99
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 28.3 bits (60), Expect = 1.0
Identities = 11/38 (28%), Positives = 20/38 (52%)
Frame = +1
Query: 244 ISDASKSSVDGCLPKWAIERYSRQILLSDIGVEGQVKI 357
+ DA ++ CL W +ER ++ ++ SD V + I
Sbjct: 375 VRDALSMAIYNCLFDWIVERVNKALVTSDNSVSNSIGI 412
>SPAC23C4.14 |alg1||mannosyltransferase complex subunit Alg1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 424
Score = 27.5 bits (58), Expect = 1.8
Identities = 30/112 (26%), Positives = 48/112 (42%), Gaps = 7/112 (6%)
Frame = +1
Query: 235 SQPISDASKSSVDGCLPKWAIERYSRQILLSDIGV--EGQVKICSAKVLIVGAGGLGCP- 405
SQ I V C+P +IE Y + + +D+GV + +V G G P
Sbjct: 293 SQYIKKHPLHKVRFCMPWLSIEDYPQVMACADLGVCLHTSSSGLDLPMKVVDLFGCGVPV 352
Query: 406 -AAMYLAGAGI---GEIGIVDYDAVDLTNVHRQLLHHESNENTSKAFSAAES 549
A Y + + GE G++ D+ L+ + LL H + N+ K + ES
Sbjct: 353 IALSYPTISELVHDGENGLIVNDSKALSKKMQYLLTHANELNSLKLGALKES 404
>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1024
Score = 26.6 bits (56), Expect = 3.2
Identities = 14/41 (34%), Positives = 24/41 (58%)
Frame = +1
Query: 199 SDQQSSKELNNHSQPISDASKSSVDGCLPKWAIERYSRQIL 321
S++++ K + N +PIS S S+V + ++ R RQIL
Sbjct: 567 SNKEAVKPIKNKPKPISFESLSAVGNLIISDSLSRIVRQIL 607
>SPBC3F6.03 |trr1|caf4|thioredoxin reductase
Trr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 322
Score = 26.2 bits (55), Expect = 4.2
Identities = 15/34 (44%), Positives = 22/34 (64%)
Frame = +1
Query: 367 KVLIVGAGGLGCPAAMYLAGAGIGEIGIVDYDAV 468
KV+I+G+G G AA+YLA GE+ V Y+ +
Sbjct: 5 KVVIIGSGPAGHTAAIYLAR---GELKPVMYEGM 35
>SPBC19C2.05 |ran1|pat1|serine/threonine protein kinase
Ran1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 470
Score = 25.4 bits (53), Expect = 7.3
Identities = 13/35 (37%), Positives = 16/35 (45%)
Frame = -2
Query: 455 STMPISPMPAPARYMAAGHPRPPAPTISTFAEQIL 351
S MPI+P P P G+P P T F +L
Sbjct: 417 SYMPITPTPYPNNAKIFGYPNQPPLTPIPFTGFVL 451
>SPCC23B6.01c |||oxysterol binding protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 489
Score = 25.0 bits (52), Expect = 9.7
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = -1
Query: 453 HDAYFANARPGKVHGSRTSQ 394
HD Y +ARP + G R SQ
Sbjct: 296 HDVYLVSARPLEEQGERESQ 315
>SPAC20G8.04c |||mitochondrial electron transfer
flavoprotein-ubiquinone
oxidoreductase|Schizosaccharomyces pombe|chr 1|||Manual
Length = 632
Score = 25.0 bits (52), Expect = 9.7
Identities = 8/12 (66%), Positives = 11/12 (91%)
Frame = -3
Query: 190 FLIPEVHFPFPL 155
FLIP++HFP P+
Sbjct: 171 FLIPKLHFPIPV 182
>SPAC30C2.05 |erv14||cornichon family protein
Erv14|Schizosaccharomyces pombe|chr 1|||Manual
Length = 141
Score = 25.0 bits (52), Expect = 9.7
Identities = 11/36 (30%), Positives = 15/36 (41%)
Frame = -1
Query: 591 VVWCDFNFRVYTSQRLSGRERLAGVLVAFMVQQLSV 484
V W N+ YT RL+G L + M L +
Sbjct: 4 VSWGSLNYLAYTFYRLNGANMLLQIFCVIMFSDLEM 39
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,553,223
Number of Sequences: 5004
Number of extensions: 50781
Number of successful extensions: 167
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 167
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 299817502
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -