Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-3625
(462 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC19C2.09 |sre1||sterol regulatory element binding protein Sre... 30 0.20
SPBC215.05 |gpd1||glycerol-3-phosphate dehydrogenase Gpd1|Schizo... 30 0.20
SPAC11E3.08c |nse6||Smc5-6 complex non-SMC subunit Nse6|Schizosa... 27 1.1
SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos... 27 1.1
SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9... 27 1.8
SPBC19G7.10c |||topoisomerase associated protein |Schizosaccharo... 26 2.4
SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo... 26 3.2
SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9 |Schiz... 26 3.2
SPAC56F8.10 |met9|met5|methylenetetrahydrofolate reductase Met9|... 25 4.3
SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy... 25 4.3
SPCC162.06c |||vacuolar sorting protein Vps60|Schizosaccharomyce... 25 4.3
SPBPB2B2.05 |||GMP synthase [glutamine-hydrolyzing] |Schizosacch... 25 5.6
SPBC19F5.05c |ppp1|SPBC25D12.01c|pescadillo-family BRCT domain p... 25 5.6
SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces pom... 25 5.6
SPBC1709.07 |erg27||3-keto sterol reductase |Schizosaccharomyces... 24 9.8
SPAC1A6.04c |plb1||phospholipase B homolog Plb1|Schizosaccharomy... 24 9.8
SPAC1952.08c |||pyridoxamine 5'-phosphate oxidase |Schizosacchar... 24 9.8
SPBC23E6.04c |utp10||U3 snoRNP-associated protein Utp10 |Schizos... 24 9.8
>SPBC19C2.09 |sre1||sterol regulatory element binding protein
Sre1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 900
Score = 29.9 bits (64), Expect = 0.20
Identities = 22/84 (26%), Positives = 38/84 (45%)
Frame = -2
Query: 380 LTP*SYTVPRKMLLVPTLLEPSLTMSSIDSGFSAIYIWLNLLSPCAMRKLATRALFSFTF 201
L P + ++ R LL +L P T S++ +A + N L C+ K ++
Sbjct: 731 LCPKNSSIIRHALLAKLVLFPENTADSLNEVLAA---YKNTLDLCSQDKRKQSSVLKINL 787
Query: 200 NRLFSSDSTDSLLCPKLGDASVKE 129
++LF+ S SL +LG V +
Sbjct: 788 SKLFTLHSCLSLALQRLGYGDVSK 811
>SPBC215.05 |gpd1||glycerol-3-phosphate dehydrogenase
Gpd1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 385
Score = 29.9 bits (64), Expect = 0.20
Identities = 14/40 (35%), Positives = 20/40 (50%), Gaps = 2/40 (5%)
Frame = -1
Query: 441 KVCG--AARHGHIARCAARVW*FNSIVVYGAQKNAVSAHF 328
K+CG A HGH R R+W F + Y +K ++ F
Sbjct: 39 KICGENARAHGHHFRSKVRMWVFEEEIEYKGEKRKLTEVF 78
>SPAC11E3.08c |nse6||Smc5-6 complex non-SMC subunit
Nse6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 522
Score = 27.5 bits (58), Expect = 1.1
Identities = 17/70 (24%), Positives = 33/70 (47%), Gaps = 3/70 (4%)
Frame = -2
Query: 338 VPTLLEPSLTMSSIDSGFSAIYIWLNLLSPC--AMRKLATRALFSFTFNRLFSSDSTD-S 168
+P L P +S+I + +W+ +LS + +K + F+ + +D +D S
Sbjct: 318 LPISLNPDFLISNIHKVSESPRVWVTILSSLSRSCQKFRKKIAFTLFVGKQSKNDDSDFS 377
Query: 167 LLCPKLGDAS 138
LC +L + S
Sbjct: 378 SLCQRLDEIS 387
>SPCC1322.12c |bub1||serine/threonine protein kinase
Bub1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1044
Score = 27.5 bits (58), Expect = 1.1
Identities = 13/43 (30%), Positives = 21/43 (48%)
Frame = +1
Query: 277 IAEKPLSIDDIVKEGSNKVGTNSIFLGTVYDYGVKSPNAGSTS 405
I +PL + + K + + S +LGT S N+G+TS
Sbjct: 396 IFNQPLRSESLEKSSKSPISAQSSYLGTPLKNDENSSNSGATS 438
>SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit
Srb9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1223
Score = 26.6 bits (56), Expect = 1.8
Identities = 14/42 (33%), Positives = 23/42 (54%)
Frame = +1
Query: 301 DDIVKEGSNKVGTNSIFLGTVYDYGVKSPNAGSTSSNVTMTR 426
D I + G NK S+ + T+ + SPN+ +T+SN T+
Sbjct: 891 DSIKRLGDNKFEDKSLRICTIPNSIFDSPNSHTTNSNSFFTK 932
>SPBC19G7.10c |||topoisomerase associated protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 744
Score = 26.2 bits (55), Expect = 2.4
Identities = 21/61 (34%), Positives = 31/61 (50%), Gaps = 2/61 (3%)
Frame = -2
Query: 458 LNSLISKFAVPRVMVTLLDVLPAFGDLTP*SYTVPRKMLLVPTLLEPSLTMSSID--SGF 285
L L+S A R T L + A G + + PR++L V EP+ + SS++ SGF
Sbjct: 392 LQRLVSS-AKERPKATQLSLEGALGKIAVNTVRTPRQLLNVKRPTEPASSNSSLNNFSGF 450
Query: 284 S 282
S
Sbjct: 451 S 451
>SPBC4.03c |||COPII-coated vesicle component Sfb3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 25.8 bits (54), Expect = 3.2
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = -2
Query: 380 LTP*SYTVPRKMLLVPTLLEPSLTMSSIDSG 288
LTP +P+ ++L+P L+ L S+I SG
Sbjct: 673 LTPGQLVLPKNLVLLPILILAVLKSSAIRSG 703
>SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 322
Score = 25.8 bits (54), Expect = 3.2
Identities = 14/52 (26%), Positives = 25/52 (48%)
Frame = +1
Query: 304 DIVKEGSNKVGTNSIFLGTVYDYGVKSPNAGSTSSNVTMTRGTANFDIKEFK 459
D +KE + K +S+F ++Y PNA N T ++D++E +
Sbjct: 64 DSIKEKTEKTKLSSLFYAGKHEYFCVYPNASLIKQNSTT---EPSYDLQELR 112
>SPAC56F8.10 |met9|met5|methylenetetrahydrofolate reductase
Met9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 603
Score = 25.4 bits (53), Expect = 4.3
Identities = 13/43 (30%), Positives = 24/43 (55%), Gaps = 1/43 (2%)
Frame = -2
Query: 356 PRKMLLVPTLLEPSLTMSSIDSGFSAIYIWLNLLSPCAM-RKL 231
P + ++ PT++E + ++ D +S W N SP ++ RKL
Sbjct: 514 PNREIIQPTIVESTSFLAWKDEAYSLGMEWANAYSPDSISRKL 556
>SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 541
Score = 25.4 bits (53), Expect = 4.3
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = -2
Query: 428 PRVMVTLLDVLPAFGDLTP*SYTVPRKMLLVPTL 327
P V++ V AF +TP YTVP+ +++PTL
Sbjct: 384 PPVLMVPYRVKKAF-PITP-DYTVPKDAMVIPTL 415
>SPCC162.06c |||vacuolar sorting protein Vps60|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 210
Score = 25.4 bits (53), Expect = 4.3
Identities = 18/49 (36%), Positives = 28/49 (57%), Gaps = 2/49 (4%)
Frame = +1
Query: 130 SLTEASPSLGQSSESVESDENKRLNVKLN--NARVANLRIAHGDNKLSQ 270
SLT+A SL + S+SVE + +L+ +L+ ++AN R G L Q
Sbjct: 16 SLTDAIDSLDKRSDSVEV-KIAKLDAQLSVFQQKIANTRPGPGQTALKQ 63
>SPBPB2B2.05 |||GMP synthase [glutamine-hydrolyzing]
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 237
Score = 25.0 bits (52), Expect = 5.6
Identities = 17/67 (25%), Positives = 34/67 (50%)
Frame = +1
Query: 217 NARVANLRIAHGDNKLSQMYIAEKPLSIDDIVKEGSNKVGTNSIFLGTVYDYGVKSPNAG 396
N R H +K + Y+A K ++ +K N +G+N I + +++D G+K+ G
Sbjct: 101 NVSSCGFRDIHRPSK-PRHYLAHKVMAKPGKLK---NILGSNVIDVNSIHDQGIKTLGMG 156
Query: 397 STSSNVT 417
S+ ++
Sbjct: 157 LQSTVIS 163
>SPBC19F5.05c |ppp1|SPBC25D12.01c|pescadillo-family BRCT domain
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 607
Score = 25.0 bits (52), Expect = 5.6
Identities = 13/31 (41%), Positives = 17/31 (54%)
Frame = +1
Query: 241 IAHGDNKLSQMYIAEKPLSIDDIVKEGSNKV 333
I HG+NK ++ IA I DIV +N V
Sbjct: 274 IVHGNNKNARKNIATLKSKIRDIVNSDANVV 304
>SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1562
Score = 25.0 bits (52), Expect = 5.6
Identities = 13/40 (32%), Positives = 22/40 (55%)
Frame = +1
Query: 247 HGDNKLSQMYIAEKPLSIDDIVKEGSNKVGTNSIFLGTVY 366
+G KL + E PLS +D++ GSN T+ +F ++
Sbjct: 508 NGSMKLDSIQ-GEIPLSNNDVLYRGSNLRNTSELFALVIF 546
>SPBC1709.07 |erg27||3-keto sterol reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 338
Score = 24.2 bits (50), Expect = 9.8
Identities = 7/21 (33%), Positives = 15/21 (71%)
Frame = -3
Query: 118 MHSSNRDKSVRAKQSITRFSH 56
+H++ D+S++ ++TRF H
Sbjct: 282 LHATKEDQSIKWGAAVTRFGH 302
>SPAC1A6.04c |plb1||phospholipase B homolog Plb1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 613
Score = 24.2 bits (50), Expect = 9.8
Identities = 13/56 (23%), Positives = 26/56 (46%)
Frame = -1
Query: 396 ARVW*FNSIVVYGAQKNAVSAHFVGALFDYVVYR*RFFGNIHLAQFIIAVCDAQIG 229
+++W FN ++Y +AHF ++ + V + NI L+ + V +G
Sbjct: 181 SKLWAFNHPLMYPKSAIVFNAHFYSSIMNEVAEKANAGFNISLSDYWGRVISRTLG 236
>SPAC1952.08c |||pyridoxamine 5'-phosphate oxidase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 24.2 bits (50), Expect = 9.8
Identities = 8/29 (27%), Positives = 19/29 (65%)
Frame = +1
Query: 157 GQSSESVESDENKRLNVKLNNARVANLRI 243
G + + VE ++ + + +KL +AR+ + R+
Sbjct: 147 GNTKQYVEGEDMRIIKIKLESARICDQRL 175
>SPBC23E6.04c |utp10||U3 snoRNP-associated protein Utp10
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1649
Score = 24.2 bits (50), Expect = 9.8
Identities = 13/43 (30%), Positives = 21/43 (48%), Gaps = 2/43 (4%)
Frame = -3
Query: 151 WATPRLRNLNLMHSSNRDKSVRAKQSITRFSHMICF--NLQFQ 29
WA + L HSSN D +V + R S+ + + ++ FQ
Sbjct: 211 WAGTMMEVLVAWHSSNEDPNVLLDRFFLRVSYAVSYVSSIDFQ 253
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.317 0.131 0.370
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,827,917
Number of Sequences: 5004
Number of extensions: 34317
Number of successful extensions: 93
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 90
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 93
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 174340060
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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