Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-2462
(713 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual 67 2e-12
SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit C... 31 0.22
SPBC713.03 |||D-lactate dehydrogenase |Schizosaccharomyces pombe... 27 2.7
SPBC2F12.12c |||conserved eukaryotic protein|Schizosaccharomyces... 26 4.7
SPAC4F8.07c |hxk2||hexokinase 2 |Schizosaccharomyces pombe|chr 1... 26 6.1
SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence orphan|Schizos... 25 8.1
SPBC8D2.02c |||vacuolar sorting protein Vps68|Schizosaccharomyce... 25 8.1
>SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 1279
Score = 67.3 bits (157), Expect = 2e-12
Identities = 31/106 (29%), Positives = 59/106 (55%)
Frame = +2
Query: 2 IVIAGEKNGVLAAKAQIEQIHEEMVKKCATVRVEVPKSQHKYVIGARGTTIQEILKETGV 181
IV++GEK+ V ++ EE+++ + +P+ QH+++ G +G IQ+IL+++G
Sbjct: 377 IVLSGEKSAVRECALYLQGQAEELLRTTIPTMLPIPRRQHRFINGEKGVGIQDILRKSGC 436
Query: 182 SVEMPPTDSPTGTITLHGPHNKIGLALSKVCEKANSVKTATVDAPT 319
SV +PP + + +++ GP I + E+ANS V+ T
Sbjct: 437 SVILPPINGDSDVVSVRGPALNISEGIRLTLERANSTIVDAVNITT 482
Score = 45.2 bits (102), Expect = 9e-06
Identities = 31/111 (27%), Positives = 55/111 (49%), Gaps = 2/111 (1%)
Frame = +2
Query: 2 IVIAGEKNGVLAAKAQIEQIHEEMVKKCATVRVEVPKSQHKYVIGARGTTIQEILKETGV 181
+V+ G+K V A + ++ +I EE+ K ++EVP+ +IG G+T ++I ++T
Sbjct: 1012 VVLRGDKEIVEALETRLLEIVEEL-KNQVEEKIEVPQRCISSIIGRMGSTRRDIERKTST 1070
Query: 182 SVEMPPTDSP--TGTITLHGPHNKIGLALSKVCEKANSVKTATVDAPTWIH 328
+ +P P T TIT+ G A + EK S T + P ++
Sbjct: 1071 MLNIPNVLDPEETVTITIVGSPENCEKAKEMIQEKVASQYTQMITVPDTVY 1121
Score = 42.3 bits (95), Expect = 7e-05
Identities = 23/99 (23%), Positives = 48/99 (48%), Gaps = 1/99 (1%)
Frame = +2
Query: 2 IVIAGEKNGVLAAKAQIEQIHEEMVKKCATVRVEVPKSQHKYVIGARGTTIQEILKETGV 181
+VI G K V AAK ++ +++E T +++P V+G G+T++ I + V
Sbjct: 855 VVIRGGKKSVAAAKQELLELYEYEKSIAYTSTIDIPSKAVSRVVGRNGSTVENIRTQFDV 914
Query: 182 SVEMPPTDS-PTGTITLHGPHNKIGLALSKVCEKANSVK 295
+++ + T +++ G + A+ ++ A VK
Sbjct: 915 KIDIGDVSTEETTPVSVRGAKADVENAIKEISAIAEEVK 953
Score = 40.3 bits (90), Expect = 3e-04
Identities = 45/210 (21%), Positives = 87/210 (41%), Gaps = 20/210 (9%)
Frame = +2
Query: 2 IVIAGEKNGVLAAKAQIEQIHEEMVKKCATVRVEVPKSQHKYVIGARGTTIQEILKETGV 181
I I G K V A+I+ E ++ +RV +P H+ +IG+ G ++ + ++ V
Sbjct: 770 IRIQGIKKNVEETAARIKSQIEALIDD-TILRVNIPNDFHRQLIGSNGKYVRRLEEKFSV 828
Query: 182 SVEMPPTDS-----------PTGT--ITLHGPHNKIGLALSKVCEKANSVK----TATVD 310
V P D PT + + G + A ++ E K T+T+D
Sbjct: 829 RVRFPREDDSSNSTGNELMKPTSPDEVVIRGGKKSVAAAKQELLELYEYEKSIAYTSTID 888
Query: 311 APTW-IHXXXXXXXXXXXXXXTQDFSKVHVDITHSEDK--VKIDGPPEEVERAQVELDNF 481
P+ + TQ K+ + +E+ V + G +VE A E+
Sbjct: 889 IPSKAVSRVVGRNGSTVENIRTQFDVKIDIGDVSTEETTPVSVRGAKADVENAIKEISAI 948
Query: 482 VKNLLATLTYVELSVDPKFFKHIIGKNGNQ 571
+ + + V + +D ++ +++IG NG++
Sbjct: 949 AEEVKNLVEKV-IKIDREYHRYLIGPNGSK 977
Score = 38.7 bits (86), Expect = 8e-04
Identities = 39/192 (20%), Positives = 75/192 (39%), Gaps = 14/192 (7%)
Frame = +2
Query: 32 LAAKAQIEQIHEEMVKKCATVRVEVPKSQHKYVIGARGTTIQEIL--KETGVSVEMPPT- 202
LA A+ + E K + ++VP HK+++G +GTT+ I+ E V V++
Sbjct: 629 LADLAESVKSSAEASAKIVSEIIQVPSVYHKHIVGPKGTTLNAIIGKSEENVIVQLGKVS 688
Query: 203 ---DSPTGTITLHGPHNKIGLALSKVCEKANSVKTATV--------DAPTWIHXXXXXXX 349
DS + + G + +S++ + K + D P
Sbjct: 689 YRPDSTDDDVYIRGFSKDVERVVSEIKQVVRDAKNHEILHSHVEEFDFPAQYSKNVIGKN 748
Query: 350 XXXXXXXTQDFSKVHVDITHSEDKVKIDGPPEEVERAQVELDNFVKNLLATLTYVELSVD 529
+D V I E ++I G + VE + + ++ L+ T + +++
Sbjct: 749 GSNVSSLREDLG---VQINVEEGHIRIQGIKKNVEETAARIKSQIEALIDD-TILRVNIP 804
Query: 530 PKFFKHIIGKNG 565
F + +IG NG
Sbjct: 805 NDFHRQLIGSNG 816
Score = 38.3 bits (85), Expect = 0.001
Identities = 21/67 (31%), Positives = 34/67 (50%)
Frame = +2
Query: 2 IVIAGEKNGVLAAKAQIEQIHEEMVKKCATVRVEVPKSQHKYVIGARGTTIQEILKETGV 181
+ + G K V A +I I EE VK +++ + H+Y+IG G+ +Q +KE G
Sbjct: 929 VSVRGAKADVENAIKEISAIAEE-VKNLVEKVIKIDREYHRYLIGPNGSKLQNTIKECGG 987
Query: 182 SVEMPPT 202
S + T
Sbjct: 988 STDKTET 994
Score = 27.1 bits (57), Expect = 2.7
Identities = 19/57 (33%), Positives = 31/57 (54%), Gaps = 4/57 (7%)
Frame = +2
Query: 410 SEDKVKIDGPPEEVERAQVELDNFV---KNLLATLTYVE-LSVDPKFFKHIIGKNGN 568
++D V I G ++VER E+ V KN ++VE ++ K++IGKNG+
Sbjct: 694 TDDDVYIRGFSKDVERVVSEIKQVVRDAKNHEILHSHVEEFDFPAQYSKNVIGKNGS 750
Score = 25.8 bits (54), Expect = 6.1
Identities = 8/24 (33%), Positives = 18/24 (75%)
Frame = +3
Query: 549 LLARMGTNINRLKDETGVVININE 620
++ + G+N++ L+++ GV IN+ E
Sbjct: 744 VIGKNGSNVSSLREDLGVQINVEE 767
>SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit
Cct6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 535
Score = 30.7 bits (66), Expect = 0.22
Identities = 18/74 (24%), Positives = 31/74 (41%)
Frame = +2
Query: 20 KNGVLAAKAQIEQIHEEMVKKCATVRVEVPKSQHKYVIGARGTTIQEILKETGVSVEMPP 199
KNG++A + + E + C V + V+G G+ + L E +
Sbjct: 306 KNGIMALRRAKRRNMERLQLACGGVAQNSVDDLNPEVLGWAGSVYERTLGEEKYTFVEDV 365
Query: 200 TDSPTGTITLHGPH 241
D + TI +HGP+
Sbjct: 366 KDPKSATILIHGPN 379
>SPBC713.03 |||D-lactate dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 526
Score = 27.1 bits (57), Expect = 2.7
Identities = 18/67 (26%), Positives = 32/67 (47%), Gaps = 1/67 (1%)
Frame = +2
Query: 32 LAAKAQIEQIHEEMVKKCATVRVEV-PKSQHKYVIGARGTTIQEILKETGVSVEMPPTDS 208
LA K + Q E++K C ++ V P+ + ++G EI+ G+ ++ D
Sbjct: 99 LALKPKTTQQVSEILKYCNQKKLAVVPQGGNTGLVGGSVPVFDEIVLNLGLMNQIHTFDE 158
Query: 209 PTGTITL 229
+G ITL
Sbjct: 159 ISGVITL 165
>SPBC2F12.12c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 517
Score = 26.2 bits (55), Expect = 4.7
Identities = 14/43 (32%), Positives = 25/43 (58%)
Frame = +2
Query: 440 PEEVERAQVELDNFVKNLLATLTYVELSVDPKFFKHIIGKNGN 568
PEE + EL++ V N++ + TY+ +S+ + K + KN N
Sbjct: 298 PEEQADYEKELNDHV-NIVKSSTYIPISIPTQKQKPTLPKNSN 339
>SPAC4F8.07c |hxk2||hexokinase 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 455
Score = 25.8 bits (54), Expect = 6.1
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = +3
Query: 27 VCWLQRLKSSKYMKKWSRNV 86
VCW+Q L KY K S++V
Sbjct: 76 VCWIQLLGDGKYEMKHSKSV 95
>SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence
orphan|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1563
Score = 25.4 bits (53), Expect = 8.1
Identities = 17/58 (29%), Positives = 23/58 (39%)
Frame = +2
Query: 146 TTIQEILKETGVSVEMPPTDSPTGTITLHGPHNKIGLALSKVCEKANSVKTATVDAPT 319
TT+ ETG + T PT T + + +NS+ T TVD PT
Sbjct: 646 TTVPHSGNETGPTTVYVETPYPTTVTTTTTVGYSGSVTTTLTGSGSNSIVTETVDVPT 703
>SPBC8D2.02c |||vacuolar sorting protein Vps68|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 170
Score = 25.4 bits (53), Expect = 8.1
Identities = 13/42 (30%), Positives = 24/42 (57%), Gaps = 2/42 (4%)
Frame = -2
Query: 478 VVKFNLSTLHFFRRSINFYF--VFRMSYVYMYL*KVLSNLFN 359
+ +F LS+LH RS+ YF + S V++++ L + F+
Sbjct: 8 IFRFRLSSLHVSSRSLGVYFAGIMFASAVWVFVDAALYSAFD 49
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,082,666
Number of Sequences: 5004
Number of extensions: 65677
Number of successful extensions: 210
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 194
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 209
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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