Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-2323
(714 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC409.06 |uch2||ubiquitin C-terminal hydrolase Uch2|Schizosacc... 169 4e-43
SPAC27F1.03c |uch1||ubiquitin C-terminal hydrolase Uch1|Schizosa... 66 6e-12
SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosacch... 27 2.0
SPBC530.06c |||translation initiation factor eIF3 alpha subunit ... 27 2.7
SPBC609.03 |||WD repeat protein, human IQWD1 family|Schizosaccha... 26 4.7
SPBC14C8.04 |||acetolactate synthase regulatory unit|Schizosacch... 26 4.7
SPAC30D11.07 |nth1||DNA endonuclease III|Schizosaccharomyces pom... 26 4.7
SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 26 6.1
SPCC1223.08c |dfr1||dihydrofolate reductase Dfr1|Schizosaccharom... 26 6.1
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 25 8.1
SPBC16C6.13c |sec27||coatomer beta' subunit |Schizosaccharomyces... 25 8.1
SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce... 25 8.1
>SPBC409.06 |uch2||ubiquitin C-terminal hydrolase
Uch2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 300
Score = 169 bits (410), Expect = 4e-43
Identities = 86/190 (45%), Positives = 119/190 (62%), Gaps = 2/190 (1%)
Frame = +1
Query: 124 WCLLESDPGVFTQLIHKFGAKGVQVEELWTIEDSMFENLRPVHGLIFLFKYVNYEEPAGP 303
W +ESD GVFT LI G K V+V+EL++++ ++G+IFLFK+ + +
Sbjct: 3 WTTIESDAGVFTDLIENLGVKDVEVDELYSLDVDSLRQFPDIYGIIFLFKWNSKVDKPDG 62
Query: 304 IVSDDRLDKIFFAKQVINNACATQAVISLLLNCKHPD-LELGPELTKLKEFSMTFDSRMR 480
+ D +D IFFAKQVINNACATQA++S+LLN H D ++LG L++ K+FS T ++
Sbjct: 63 TMDYDSMDNIFFAKQVINNACATQALLSVLLN--HSDEIDLGTTLSEFKDFSKTLPPELK 120
Query: 481 GLSLSNSQTIRSAHNSMAQQPLFEVDPKMPAKDED-AYHFIGYMPIDGRLYELDGLQEGP 657
G +L NS+ IR HNS A+ F + A DED YHFI Y I+ YELDGLQ P
Sbjct: 121 GEALGNSEHIRCCHNSFARSDPFISEEVRAATDEDEVYHFIAYTNINNVFYELDGLQAAP 180
Query: 658 IDHGAVAPEQ 687
I+HG+ E+
Sbjct: 181 INHGSCTKEE 190
>SPAC27F1.03c |uch1||ubiquitin C-terminal hydrolase
Uch1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 222
Score = 65.7 bits (153), Expect = 6e-12
Identities = 55/198 (27%), Positives = 87/198 (43%), Gaps = 6/198 (3%)
Frame = +1
Query: 124 WCLLESDPGVFTQLIHKFGAKGVQVEELWTIEDSMFENLRPVHGLIFLFKYVNYEE-PAG 300
W LE+ P V + K G + V +L+++E+ RPVH L+F+F + G
Sbjct: 2 WRPLENTPEVLEPYLQKIGVQDASVFDLFSLEEIPEYIPRPVHALLFVFPSSGTKTIYKG 61
Query: 301 PIVSDDRLDKIFFAKQVINNACATQAVISLLLNCK-HPDLELGPELTKLKEFSMTFDSRM 477
+ DK+ + Q I NAC T ++ + N + + + L +
Sbjct: 62 SRILPKDSDKVLWYPQTIPNACGTIGLLHAVSNGELRRKVNENDFIKSLIRTAEGSSIEE 121
Query: 478 RGLSLSNSQTIRSAHNSMAQQPLFEVDPKMPAKDEDAYHFI----GYMPIDGRLYELDGL 645
R + +S+ + + H + A PL EV+ + D HFI G D YELDG
Sbjct: 122 RAKLIEDSKELEALHAAFAGPPL-EVEGSEEDVETD-LHFICFVKGKSKDDNHFYELDGR 179
Query: 646 QEGPIDHGAVAPEQDWLD 699
QEGP+ H + E D L+
Sbjct: 180 QEGPVQHSEI--ESDLLN 195
>SPBC16D10.05 |mok13||alpha-1,3-glucan synthase
Mok13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2358
Score = 27.5 bits (58), Expect = 2.0
Identities = 22/82 (26%), Positives = 37/82 (45%), Gaps = 4/82 (4%)
Frame = +1
Query: 430 ELTKLKEFSMTFDSRMRGLSLSNSQTIRSAHNSMAQQPLFEVDPKMPAKDEDAYHFIGYM 609
E+T + + R R + S ++S ++ F +PK A D A+H+ Y
Sbjct: 341 EVTGSSSYGSIYYGRGRQPDQRPPSILTSLNSSSLKENYFLREPKANALDASAFHYSLYR 400
Query: 610 PIDGRLYELDG-LQEG---PID 663
+ R ++DG LQ G P+D
Sbjct: 401 AMT-RFLQMDGDLQVGHDLPVD 421
>SPBC530.06c |||translation initiation factor eIF3 alpha subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1173
Score = 27.1 bits (57), Expect = 2.7
Identities = 15/47 (31%), Positives = 24/47 (51%)
Frame = +1
Query: 250 HGLIFLFKYVNYEEPAGPIVSDDRLDKIFFAKQVINNACATQAVISL 390
H L LF Y EP P+++++ + F A QVI + +T S+
Sbjct: 779 HLLNNLFTVYGYVEPTKPLINENIANLFFQATQVIYSINSTSLYSSI 825
>SPBC609.03 |||WD repeat protein, human IQWD1
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 809
Score = 26.2 bits (55), Expect = 4.7
Identities = 13/34 (38%), Positives = 20/34 (58%), Gaps = 3/34 (8%)
Frame = +1
Query: 622 RLYELDGLQEGPIDHGAVAPEQDW---LDVVRPI 714
+L++LD +EG +DHG + W LD V+ I
Sbjct: 116 KLFDLDSSKEGGMDHGMETQTRCWSCALDSVKNI 149
>SPBC14C8.04 |||acetolactate synthase regulatory
unit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 289
Score = 26.2 bits (55), Expect = 4.7
Identities = 9/27 (33%), Positives = 18/27 (66%)
Frame = +1
Query: 127 CLLESDPGVFTQLIHKFGAKGVQVEEL 207
CL++++PGV ++L A+G ++ L
Sbjct: 75 CLVQNEPGVLSRLSGILAARGFNIDSL 101
>SPAC30D11.07 |nth1||DNA endonuclease III|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 355
Score = 26.2 bits (55), Expect = 4.7
Identities = 10/26 (38%), Positives = 18/26 (69%)
Frame = +1
Query: 373 QAVISLLLNCKHPDLELGPELTKLKE 450
Q +++L+L+ + D+ LGP + LKE
Sbjct: 50 QTLVALMLSSQTKDIVLGPTMRNLKE 75
>SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1085
Score = 25.8 bits (54), Expect = 6.1
Identities = 21/69 (30%), Positives = 37/69 (53%), Gaps = 2/69 (2%)
Frame = +1
Query: 394 LNCKHPDL-ELGPELTKLKEFSMTFDSRMRGLSLSN-SQTIRSAHNSMAQQPLFEVDPKM 567
LN + D+ E E T L+E ++ D++ S+SN +QT+ +S QP F+
Sbjct: 383 LNSSNADVDEEVIETTSLEEKNV--DNQEFVTSISNGNQTLEDTSHSPQTQPPFQPPYPS 440
Query: 568 PAKDEDAYH 594
A ++++YH
Sbjct: 441 KADEKNSYH 449
>SPCC1223.08c |dfr1||dihydrofolate reductase
Dfr1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 461
Score = 25.8 bits (54), Expect = 6.1
Identities = 9/22 (40%), Positives = 13/22 (59%)
Frame = +1
Query: 262 FLFKYVNYEEPAGPIVSDDRLD 327
+L KY P GP+V+D+ D
Sbjct: 30 YLSKYAELHFPTGPVVADEEAD 51
>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1024
Score = 25.4 bits (53), Expect = 8.1
Identities = 18/80 (22%), Positives = 34/80 (42%), Gaps = 1/80 (1%)
Frame = +1
Query: 463 FDSRMRGLSLSNSQTIRSAHNSMAQQPLFEVDPKMPAKDEDAYHFIGYMPIDGRLYELDG 642
F S + G +++R +A Q DP P + ++A F+G P YE +
Sbjct: 60 FSSTLEGNRFEELRSLREKEREVAIQNGLIDDPTKPRQLDEAVTFVGTCPDMCPEYEREQ 119
Query: 643 LQ-EGPIDHGAVAPEQDWLD 699
+ + ++ + PE +D
Sbjct: 120 REYQNNLERWEINPETGRVD 139
>SPBC16C6.13c |sec27||coatomer beta' subunit |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 796
Score = 25.4 bits (53), Expect = 8.1
Identities = 9/23 (39%), Positives = 15/23 (65%)
Frame = +1
Query: 571 AKDEDAYHFIGYMPIDGRLYELD 639
A E +++ +GY+P D R+Y D
Sbjct: 555 ANVESSFYLLGYIPRDDRIYLTD 577
>SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 817
Score = 25.4 bits (53), Expect = 8.1
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = -2
Query: 221 SSIVHSSSTCTPLAPNL*IS*VKTPGSLSRRHQ 123
SS + S+ C+P P+L ++ KT +RR Q
Sbjct: 325 SSFISSTRLCSPENPSLLVNTAKTSKMSARREQ 357
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,045,586
Number of Sequences: 5004
Number of extensions: 63805
Number of successful extensions: 169
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 160
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 165
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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