Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-2032
(754 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 35 0.011
SPAC29A4.08c |prp19|cwf8|ubiquitin-protein ligase E4 |Schizosacc... 34 0.025
SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 30 0.41
SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4 |Schi... 30 0.41
SPAC22E12.19 ||SPAC2E12.01|histone deacetylase complex subunit |... 27 2.2
SPBC3E7.05c |||conserved eukaryotic protein|Schizosaccharomyces ... 27 2.9
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 26 5.0
SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces pom... 26 6.6
SPAC26H5.04 |||vacuolar import and degradation protein Vid28|Sch... 26 6.6
SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|... 26 6.6
>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
Sen1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1687
Score = 35.1 bits (77), Expect = 0.011
Identities = 35/128 (27%), Positives = 53/128 (41%), Gaps = 13/128 (10%)
Frame = -1
Query: 364 LCSLHKTGKSF----MACLRIATSSSSHKSNWASIACSDKVGKLLKNFAMN*DAKRFSLS 197
L +L KT +S M+CLRI +SSS W++ +V LKN N A F +S
Sbjct: 254 LLNLVKTSESLSSQQMSCLRICCNSSSF---WSAADSFKEVSSFLKNLLKNVTATPFEVS 310
Query: 196 DPFTAASISKYSFSCCE---------ISSFAAAKIS*FLYNFSILSPAIFLCPLTFVKIS 44
D A I+ + +C + F K + +S+L L L + S
Sbjct: 311 DMNWAYIIASFFRTCLNDFVSVFPEWLKGFVEEKRTIGFIIYSVLDALFDLLSLDYSSPS 370
Query: 43 LTNNNIEI 20
NN + +
Sbjct: 371 FLNNTLSL 378
>SPAC29A4.08c |prp19|cwf8|ubiquitin-protein ligase E4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 488
Score = 33.9 bits (74), Expect = 0.025
Identities = 34/125 (27%), Positives = 55/125 (44%), Gaps = 9/125 (7%)
Frame = +3
Query: 123 LAAAKDEISQHEKEYLEILAAVKGSDKEKRLASQFIAKFFNSFPTLSEQAIEAQ-FDLCE 299
L K E+S L + KE+ A + +AKF ++ T+S + IE Q ++ E
Sbjct: 94 LTETKQELSTALYSLDAALRVISRLTKERDEAREALAKFSDNIGTVSSKTIEVQEVEMGE 153
Query: 300 DDD---VAIRKQAIKDLPVLCKEHK----EHTQRIADILAQLLQSEDSTEI-NVVTNSLV 455
DD ++R K L + K + D ++QLLQ+ ST + N+ T S
Sbjct: 154 SDDQLKTSLRSTVEKTFQELSSKRKRTKLQPKWATDDAVSQLLQATPSTILENLETESTT 213
Query: 456 TILKS 470
+ S
Sbjct: 214 SFFPS 218
>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 513
Score = 29.9 bits (64), Expect = 0.41
Identities = 24/83 (28%), Positives = 38/83 (45%), Gaps = 3/83 (3%)
Frame = +3
Query: 486 LSGIFSQIHQNTDGELANEIVRERCIKFLSSKIQQLGREIINKEAEELIITECKKILEDV 665
L I SQ+ + G A E I + K+Q+ +E+I++E E I E KI +DV
Sbjct: 352 LDDIISQLMEQAQGHNAPAPAPEDVIAKM--KVQKPPKELIDEEGECTICMEMFKINDDV 409
Query: 666 VAEEFEHIME---LLTWSRLGKT 725
+ +H + W R+ T
Sbjct: 410 IQLPCKHYFHENCIKPWLRVNGT 432
>SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 865
Score = 29.9 bits (64), Expect = 0.41
Identities = 28/102 (27%), Positives = 45/102 (44%), Gaps = 8/102 (7%)
Frame = +3
Query: 375 QRIADILAQLLQSEDSTEINVVTNSLVTILKSDPKGALSGIFSQIHQNTDGELANEI--- 545
+R+ + A LL E+ + ++TNSL LKS K + S ELA ++
Sbjct: 110 KRLGYLAAMLLLDENQEVLTLLTNSLQNDLKSRDKFIVGLALSAFGNVAGPELARDLSND 169
Query: 546 VRERCI---KFLSSKIQQLGREIINKE--AEELIITECKKIL 656
+ E C ++S K +I KE E L I + ++L
Sbjct: 170 IAELCSNHHNYISKKAVLCALRVIQKEPDLESLYIEKTDELL 211
>SPAC22E12.19 ||SPAC2E12.01|histone deacetylase complex subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 661
Score = 27.5 bits (58), Expect = 2.2
Identities = 17/51 (33%), Positives = 31/51 (60%), Gaps = 2/51 (3%)
Frame = +3
Query: 141 EISQHEKEYLEILAAVKGSDKEKRLASQFIAKFFNSFP--TLSEQAIEAQF 287
+I + E+E+LEILA ++ +KE S+ A + P LSE+ +++Q+
Sbjct: 179 DIVRSEEEFLEILAKLEQQEKEASNVSE--ASRIATIPPMILSEEEVKSQY 227
>SPBC3E7.05c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 550
Score = 27.1 bits (57), Expect = 2.9
Identities = 12/44 (27%), Positives = 24/44 (54%)
Frame = +3
Query: 159 KEYLEILAAVKGSDKEKRLASQFIAKFFNSFPTLSEQAIEAQFD 290
++++ L D+ LAS+ I K +S P L ++ ++ +FD
Sbjct: 394 RQHVNTLKEATKDDELAALASRVIEKIVDSGPILDKEELQTKFD 437
>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 4717
Score = 26.2 bits (55), Expect = 5.0
Identities = 22/88 (25%), Positives = 37/88 (42%), Gaps = 1/88 (1%)
Frame = +3
Query: 387 DILAQLLQSEDSTEINVVTNSLVTILKSDPKGALSGIFSQIHQNTDGELANEIVRERCIK 566
D L +L ED+ + + S L+SD G ++N+D E N+ + E
Sbjct: 3931 DDLEELANEEDTANQSDLDESEARELESDMNGVTKDSVVSENENSDSEEENQDLDEEVND 3990
Query: 567 FLSSKIQQLGREIINKEAEE-LIITECK 647
L ++ ++ EE L+ TE K
Sbjct: 3991 IPEDLSNSLNEKLWDEPNEEDLLETEQK 4018
>SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 772
Score = 25.8 bits (54), Expect = 6.6
Identities = 9/24 (37%), Positives = 17/24 (70%)
Frame = -3
Query: 518 VLMNL*EDTRQSSFGITFQNCYKR 447
+L+++ E TRQ ++ + FQ YK+
Sbjct: 443 ILLDMKESTRQKNWSLFFQRLYKK 466
>SPAC26H5.04 |||vacuolar import and degradation protein
Vid28|Schizosaccharomyces pombe|chr 1|||Manual
Length = 729
Score = 25.8 bits (54), Expect = 6.6
Identities = 28/115 (24%), Positives = 56/115 (48%), Gaps = 1/115 (0%)
Frame = +3
Query: 288 DLCEDDDVAIRKQAIKDLPVLCKEHKEHTQRIADILAQLLQSEDSTEINVVTNSLVTILK 467
+LC D+D+ +++Q ++ L + +E D L +++ E +I L +
Sbjct: 518 ELCNDEDLGVQEQMLQVLRNFTCQKEES----VDFLLKMVPMELLAKI-----LLEKLES 568
Query: 468 SDPKGALSGIFSQIH-QNTDGELANEIVRERCIKFLSSKIQQLGREIINKEAEEL 629
+P I+ +H +DGEL + I+R+ +K+ L +EI+ +EA+ L
Sbjct: 569 KNPILIEPSIYILVHIAASDGELRDSILRQ-------TKLLLLVKEIMLQEAQRL 616
>SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 732
Score = 25.8 bits (54), Expect = 6.6
Identities = 15/42 (35%), Positives = 23/42 (54%), Gaps = 2/42 (4%)
Frame = -3
Query: 386 SNSLSVFFVLFA*NW--QIFYGLFTNSNVVILAQVKLGFYCL 267
S S+ FF+L Q+ YGL+TNS +I + + F C+
Sbjct: 347 SRSIFYFFLLNVSYMFVQVIYGLWTNSLGLISDAIHMAFDCI 388
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,026,181
Number of Sequences: 5004
Number of extensions: 61863
Number of successful extensions: 208
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 197
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 208
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 359287726
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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