Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-2012
(803 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1672.12c |||DUF410 family protein|Schizosaccharomyces pombe|... 69 8e-13
SPBC15D4.09c |||cystathionine gamma-synthase |Schizosaccharomyce... 32 0.11
SPBC3E7.06c |||membrane transporter|Schizosaccharomyces pombe|ch... 27 2.4
SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces... 27 3.1
SPAC26A3.02 |myh1|myh|adenine DNA glycosylase |Schizosaccharomyc... 27 4.1
SPCC613.09 |sen54||tRNA-splicing endonuclease subunit Sen54 |Sch... 27 4.1
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo... 26 5.5
SPCC297.03 |ssp1||serine/threonine protein kinase Ssp1 |Schizosa... 26 7.2
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 26 7.2
SPBC1773.14 |arg7||argininosuccinate lyase |Schizosaccharomyces ... 25 9.5
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 25 9.5
SPAC22H10.09 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 9.5
SPAC2G11.05c |||BRO1 domain protein|Schizosaccharomyces pombe|ch... 25 9.5
SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1 |Schiz... 25 9.5
>SPCC1672.12c |||DUF410 family protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 303
Score = 68.9 bits (161), Expect = 8e-13
Identities = 40/136 (29%), Positives = 67/136 (49%)
Frame = +1
Query: 151 YEAHQMYRTLYFRYLTQKKYPEXXXXXXXXXXXXXERDQQGSGADLAILFVEVLTKSETK 330
YE HQM RTL R + KK+ + E +Q GS ADLAI ++V K+
Sbjct: 17 YEGHQMLRTLVNRQVKAKKHDDAVALLYSGAKTLFEIEQTGSAADLAIYMLDVYEKASYA 76
Query: 331 PNEEWVSKLAKLFELISSSVPERETFLTNSIKWSMDNNKKGDPLLHKKIAEVFWKEKKYT 510
+ + ++ L L + R+ ++ ++WS +GD +H +A +F K K+
Sbjct: 77 ASLDNKARALTLLGLFPAEEGARKQYVKRLLEWSKSAGPQGDKDVHFAVATMFVKWKEPA 136
Query: 511 AAHRHFLHSSDGSAYA 558
+A +HF+ ++ SA A
Sbjct: 137 SAEKHFVLGNEKSARA 152
>SPBC15D4.09c |||cystathionine gamma-synthase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 610
Score = 31.9 bits (69), Expect = 0.11
Identities = 17/71 (23%), Positives = 36/71 (50%), Gaps = 1/71 (1%)
Frame = +1
Query: 430 SMDNNKKGDPLLH-KKIAEVFWKEKKYTAAHRHFLHSSDGSAYANMLIELHTTKGLKSEI 606
S+D N+K +H ++ V + K + A + + H+ DG + LH+ K ++S
Sbjct: 129 SIDRNRKYTEEIHIPQVYSVLFPSKYFGIAKQFWQHTGDGISSRRAAAFLHSYKKIQSIS 188
Query: 607 DLFIAQACVAN 639
+ +AQ +++
Sbjct: 189 EFLVAQRSISH 199
>SPBC3E7.06c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 577
Score = 27.5 bits (58), Expect = 2.4
Identities = 14/28 (50%), Positives = 15/28 (53%)
Frame = -3
Query: 795 RTCLCSTALSKNHKKFNSGNIYGGPLLF 712
RTCL S + H N G IYG PL F
Sbjct: 349 RTCLSSYLCNFFHSVANFGWIYGMPLFF 376
>SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1526
Score = 27.1 bits (57), Expect = 3.1
Identities = 30/106 (28%), Positives = 46/106 (43%), Gaps = 5/106 (4%)
Frame = +1
Query: 310 LTKSETKPNEEWVSKLAKLFELIS---SSVPERETFLTNSIKWSMDNNKKGDPLLHKKIA 480
L SE K VS L K +S S VPE E +TN + K + K+ A
Sbjct: 1221 LASSENKDLSNKVSSLTKQVNELSPKASKVPELERKITNLMHEYSQLGKTFED--EKRKA 1278
Query: 481 EVFWKEKKYTAAHRHFLHSSDGSA--YANMLIELHTTKGLKSEIDL 612
+ ++ + + + L S Y +L E+ TT+ L+SE+ L
Sbjct: 1279 LIASRDNEELRSLKSELESKRKLEVEYQKVLEEVKTTRSLRSEVTL 1324
>SPAC26A3.02 |myh1|myh|adenine DNA glycosylase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 461
Score = 26.6 bits (56), Expect = 4.1
Identities = 10/34 (29%), Positives = 20/34 (58%)
Frame = +1
Query: 304 EVLTKSETKPNEEWVSKLAKLFELISSSVPERET 405
E + SE P E+W + +L+E++ S + ++T
Sbjct: 42 ECIPPSEDSPLEDWEQPVQRLYEVLVSEIMLQQT 75
>SPCC613.09 |sen54||tRNA-splicing endonuclease subunit Sen54
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 384
Score = 26.6 bits (56), Expect = 4.1
Identities = 12/31 (38%), Positives = 18/31 (58%)
Frame = +2
Query: 494 RKKSTQLLIDTFYTHRMVQHMLTCL*SYTQL 586
R++ + LL DTFYT+ V H L + Y +
Sbjct: 227 RRRQSILLHDTFYTYEEVYHDLQIVRGYVPI 257
>SPAC1805.15c |pub2||ubiquitin-protein ligase
Pub2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 671
Score = 26.2 bits (55), Expect = 5.5
Identities = 14/31 (45%), Positives = 20/31 (64%)
Frame = -1
Query: 470 LCRSGSPFLLLSIDHFIEFVRKVSRSGTLEL 378
L RS P+LL+S+D +F++ SGTL L
Sbjct: 23 LLRSLKPYLLISVDD-DQFIKTNVASGTLRL 52
>SPCC297.03 |ssp1||serine/threonine protein kinase Ssp1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 652
Score = 25.8 bits (54), Expect = 7.2
Identities = 9/24 (37%), Positives = 15/24 (62%)
Frame = +2
Query: 671 ETFLRYTETHPKIKNSKGPPYIFP 742
+ LR HP++ +S+ PP +FP
Sbjct: 15 QRLLRKNTWHPEVDDSEVPPSVFP 38
>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1778
Score = 25.8 bits (54), Expect = 7.2
Identities = 20/62 (32%), Positives = 33/62 (53%), Gaps = 5/62 (8%)
Frame = +1
Query: 331 PNEEWVSKLAKLFELISSSVPERETFLTNSIK--WSMDNN--KKGD-PLLHKKIAEVFWK 495
PN+ +L + F + S P +TF++ +K +S D+N ++GD KK+ EV
Sbjct: 986 PNDSKNKELNRHFLKVDDSAPLDDTFMSKKVKLDFSSDSNVSERGDYDDNAKKVDEVISI 1045
Query: 496 EK 501
EK
Sbjct: 1046 EK 1047
>SPBC1773.14 |arg7||argininosuccinate lyase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 461
Score = 25.4 bits (53), Expect = 9.5
Identities = 8/30 (26%), Positives = 19/30 (63%)
Frame = +1
Query: 61 CNKNIMATRGERGVVRVLDKLEASINSGQY 150
C KN+++ +++ L++++ NSGQ+
Sbjct: 51 CQKNVISEEELNSILKGLEQIQREWNSGQF 80
>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2100
Score = 25.4 bits (53), Expect = 9.5
Identities = 15/67 (22%), Positives = 29/67 (43%)
Frame = -1
Query: 509 VYFFSFQNTSAIFLCRSGSPFLLLSIDHFIEFVRKVSRSGTLELINSNNFANFETHSSLG 330
V+ Q I L S SPF+L +E++ + S + + NF+N +L
Sbjct: 1418 VFLLCAQECCRILLTDSKSPFILEVFSAILEYICQASTKTAINVSLYWNFSNDLEKLNLP 1477
Query: 329 LVSDFVK 309
++ ++
Sbjct: 1478 IILSLIR 1484
>SPAC22H10.09 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 646
Score = 25.4 bits (53), Expect = 9.5
Identities = 12/45 (26%), Positives = 23/45 (51%)
Frame = -1
Query: 140 ELIEASNLSNTLTTPLSPRVAMIFLLQFPDYSVFFRFRDHNQFTN 6
+L++++N+ ++L + +FLL DYS+F H N
Sbjct: 223 KLLKSANIRSSLNSKTFNDFRTLFLLLIRDYSLFHGNSSHCMLAN 267
>SPAC2G11.05c |||BRO1 domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 701
Score = 25.4 bits (53), Expect = 9.5
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = +2
Query: 656 VRMATETFLRYTETHPKIKNSKGPPYIF 739
V++ + FL Y + + KN+ G P IF
Sbjct: 195 VKILAKVFLLYEDAYKLFKNATGAPSIF 222
>SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 676
Score = 25.4 bits (53), Expect = 9.5
Identities = 9/24 (37%), Positives = 17/24 (70%)
Frame = +1
Query: 730 IYIPTIEFFVVLAESCRTETCTTV 801
+Y PT F+++ AE+C +++ T V
Sbjct: 126 LYAPTSAFWLLTAEACLSQSMTIV 149
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,557,190
Number of Sequences: 5004
Number of extensions: 77937
Number of successful extensions: 223
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 212
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 223
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 390427050
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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