BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-1866
(753 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC17D11.01 |nep1||nedd8 protease Nep1|Schizosaccharomyces pomb... 61 1e-10
SPBC32H8.02c |nep2|mug120|nedd8 protease Nep2|Schizosaccharomyce... 55 1e-08
SPBC19G7.09 |ulp1||SUMO deconjugating enzyme Ulp1|Schizosaccharo... 31 0.23
SPAC17A5.07c |ulp2||SUMO deconjugating cysteine peptidase Ulp2 |... 29 0.54
>SPBC17D11.01 |nep1||nedd8 protease Nep1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 420
Score = 61.3 bits (142), Expect = 1e-10
Identities = 36/128 (28%), Positives = 67/128 (52%), Gaps = 7/128 (5%)
Frame = +1
Query: 322 MAKASVVLSFHDILLHRSDIELLEGPHWLNDTIISFYFEYLEKVVF----SKESDLLFVS 489
M+ + VL D+ + D++ L+ P+W D I + E +E + F ++ +++L +
Sbjct: 1 MSSSPTVLELFDVCFKQEDVDSLKKPNWFTDVSIDYVDELIEHLWFPSYPNQANEILLLR 60
Query: 490 PEVTQCIK--MVQTEEIKTFLEPLDVNLKKFVFFALND-NNTPDMAGGSHWSLLVYSRPE 660
P + + + EE+K L +N K ++F +ND + +GGSHWSL+V S P+
Sbjct: 61 PSLVFLLAEAAISPEELKVALPKKLMNCK-YLFMPINDLDKHAAGSGGSHWSLMVASIPD 119
Query: 661 NCFFHLDS 684
++ DS
Sbjct: 120 GQCYYYDS 127
>SPBC32H8.02c |nep2|mug120|nedd8 protease Nep2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 415
Score = 54.8 bits (126), Expect = 1e-08
Identities = 35/125 (28%), Positives = 62/125 (49%), Gaps = 6/125 (4%)
Frame = +1
Query: 343 LSFHDILLHRSDIELLEGPHWLNDTIISFYFEYLEKVVFSK----ESDLLFV-SPEVTQC 507
L + ++ L ++D++ +W+ DT I F++E + + V K ES +++ P +
Sbjct: 71 LEYFEVSLRKNDVDHFRPGYWILDTNIDFFYEIMLRQVLLKRPKEESQQIYLLRPAMVFF 130
Query: 508 IKMVQTE-EIKTFLEPLDVNLKKFVFFALNDNNTPDMAGGSHWSLLVYSRPENCFFHLDS 684
+ EI++ L P + F+F +ND N + GSHWSLLV S + ++ DS
Sbjct: 131 LAQAPNPLEIESALPPAMFDAS-FIFLPINDTNECGIESGSHWSLLVVSVEKGLGWYYDS 189
Query: 685 SQATN 699
N
Sbjct: 190 MSNGN 194
>SPBC19G7.09 |ulp1||SUMO deconjugating enzyme
Ulp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 568
Score = 30.7 bits (66), Expect = 0.23
Identities = 14/54 (25%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
Frame = +1
Query: 292 KAKLFLNYTKMAKASV-VLSFHDILLHRSDIELLEGPHWLNDTIISFYFEYLEK 450
+A +LN + +S +++ +I + D+ L WLND +I+FY + +
Sbjct: 354 RALKYLNQSNSVSSSEPIITKFNIPITLKDLHTLRNRQWLNDEVINFYMNLISE 407
>SPAC17A5.07c |ulp2||SUMO deconjugating cysteine peptidase Ulp2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 652
Score = 29.5 bits (63), Expect = 0.54
Identities = 12/31 (38%), Positives = 18/31 (58%)
Frame = +1
Query: 352 HDILLHRSDIELLEGPHWLNDTIISFYFEYL 444
+ I + +D+ L +LNDTI+ FY YL
Sbjct: 359 NSIAITNTDLTRLNEGEFLNDTIVDFYLRYL 389
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,933,381
Number of Sequences: 5004
Number of extensions: 58718
Number of successful extensions: 125
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 119
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 124
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 359287726
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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