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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ceN-1866
         (753 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC17D11.01 |nep1||nedd8 protease Nep1|Schizosaccharomyces pomb...    61   1e-10
SPBC32H8.02c |nep2|mug120|nedd8 protease Nep2|Schizosaccharomyce...    55   1e-08
SPBC19G7.09 |ulp1||SUMO deconjugating enzyme Ulp1|Schizosaccharo...    31   0.23 
SPAC17A5.07c |ulp2||SUMO deconjugating cysteine peptidase Ulp2 |...    29   0.54 

>SPBC17D11.01 |nep1||nedd8 protease Nep1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 420

 Score = 61.3 bits (142), Expect = 1e-10
 Identities = 36/128 (28%), Positives = 67/128 (52%), Gaps = 7/128 (5%)
 Frame = +1

Query: 322 MAKASVVLSFHDILLHRSDIELLEGPHWLNDTIISFYFEYLEKVVF----SKESDLLFVS 489
           M+ +  VL   D+   + D++ L+ P+W  D  I +  E +E + F    ++ +++L + 
Sbjct: 1   MSSSPTVLELFDVCFKQEDVDSLKKPNWFTDVSIDYVDELIEHLWFPSYPNQANEILLLR 60

Query: 490 PEVTQCIK--MVQTEEIKTFLEPLDVNLKKFVFFALND-NNTPDMAGGSHWSLLVYSRPE 660
           P +   +    +  EE+K  L    +N K ++F  +ND +     +GGSHWSL+V S P+
Sbjct: 61  PSLVFLLAEAAISPEELKVALPKKLMNCK-YLFMPINDLDKHAAGSGGSHWSLMVASIPD 119

Query: 661 NCFFHLDS 684
              ++ DS
Sbjct: 120 GQCYYYDS 127


>SPBC32H8.02c |nep2|mug120|nedd8 protease Nep2|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 415

 Score = 54.8 bits (126), Expect = 1e-08
 Identities = 35/125 (28%), Positives = 62/125 (49%), Gaps = 6/125 (4%)
 Frame = +1

Query: 343 LSFHDILLHRSDIELLEGPHWLNDTIISFYFEYLEKVVFSK----ESDLLFV-SPEVTQC 507
           L + ++ L ++D++     +W+ DT I F++E + + V  K    ES  +++  P +   
Sbjct: 71  LEYFEVSLRKNDVDHFRPGYWILDTNIDFFYEIMLRQVLLKRPKEESQQIYLLRPAMVFF 130

Query: 508 IKMVQTE-EIKTFLEPLDVNLKKFVFFALNDNNTPDMAGGSHWSLLVYSRPENCFFHLDS 684
           +       EI++ L P   +   F+F  +ND N   +  GSHWSLLV S  +   ++ DS
Sbjct: 131 LAQAPNPLEIESALPPAMFDAS-FIFLPINDTNECGIESGSHWSLLVVSVEKGLGWYYDS 189

Query: 685 SQATN 699
               N
Sbjct: 190 MSNGN 194


>SPBC19G7.09 |ulp1||SUMO deconjugating enzyme
           Ulp1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 568

 Score = 30.7 bits (66), Expect = 0.23
 Identities = 14/54 (25%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
 Frame = +1

Query: 292 KAKLFLNYTKMAKASV-VLSFHDILLHRSDIELLEGPHWLNDTIISFYFEYLEK 450
           +A  +LN +    +S  +++  +I +   D+  L    WLND +I+FY   + +
Sbjct: 354 RALKYLNQSNSVSSSEPIITKFNIPITLKDLHTLRNRQWLNDEVINFYMNLISE 407


>SPAC17A5.07c |ulp2||SUMO deconjugating cysteine peptidase Ulp2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 652

 Score = 29.5 bits (63), Expect = 0.54
 Identities = 12/31 (38%), Positives = 18/31 (58%)
 Frame = +1

Query: 352 HDILLHRSDIELLEGPHWLNDTIISFYFEYL 444
           + I +  +D+  L    +LNDTI+ FY  YL
Sbjct: 359 NSIAITNTDLTRLNEGEFLNDTIVDFYLRYL 389


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,933,381
Number of Sequences: 5004
Number of extensions: 58718
Number of successful extensions: 125
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 119
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 124
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 359287726
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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