Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ceN-1713
         (641 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1...    33   0.035
SPAC3G9.14 |sak1||transcriptional repressor Sak1|Schizosaccharom...    30   0.25 
SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2...    30   0.25 
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit...    28   1.3  
SPBC3D6.04c |mad1||mitotic spindle checkpoint protein Mad1|Schiz...    26   4.0  
SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyce...    26   5.3  
SPBC16H5.14c ||SPBC21H7.08|short chain dehydrogenase DHRS family...    26   5.3  
SPBC543.07 |pek1|skh1, mkk1|MAP kinase kinase Pek1 |Schizosaccha...    25   9.3  
SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces ...    25   9.3  

>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 857

 Score = 33.1 bits (72), Expect = 0.035
 Identities = 23/64 (35%), Positives = 26/64 (40%)
 Frame = +1

Query: 235 PTYHAAPAVHEASSYQAIHSAPTYHVAPAGHEAPTYHAAPAAHSATTYHTAPAIHATSAI 414
           P   AAP V EA S   +H  P   VAP    AP   AAP    A +    PA+      
Sbjct: 515 PQPPAAPVVPEAPS---VHQPPAAPVAPEVPSAPQRPAAPVVPEAPSVPQRPAVPVVPEA 571

Query: 415 HQVP 426
             VP
Sbjct: 572 LSVP 575



 Score = 32.7 bits (71), Expect = 0.046
 Identities = 17/58 (29%), Positives = 27/58 (46%)
 Frame = +1

Query: 253 PAVHEASSYQAIHSAPTYHVAPAGHEAPTYHAAPAAHSATTYHTAPAIHATSAIHQVP 426
           P+V +  +   +  AP+ H  PA   AP   +AP   +A     AP++    A+  VP
Sbjct: 512 PSVPQPPAAPVVPEAPSVHQPPAAPVAPEVPSAPQRPAAPVVPEAPSVPQRPAVPVVP 569



 Score = 30.3 bits (65), Expect = 0.25
 Identities = 22/69 (31%), Positives = 29/69 (42%)
 Frame = +1

Query: 223 IQEAPTYHAAPAVHEASSYQAIHSAPTYHVAPAGHEAPTYHAAPAAHSATTYHTAPAIHA 402
           I  A    A P+V   S  Q   +AP    AP+ H+ P    AP   SA     AP +  
Sbjct: 497 ISSASQKAAQPSVITPSVPQP-PAAPVVPEAPSVHQPPAAPVAPEVPSAPQRPAAPVVPE 555

Query: 403 TSAIHQVPA 429
             ++ Q PA
Sbjct: 556 APSVPQRPA 564



 Score = 29.9 bits (64), Expect = 0.33
 Identities = 21/71 (29%), Positives = 28/71 (39%), Gaps = 3/71 (4%)
 Frame = +1

Query: 223 IQEAPTYH---AAPAVHEASSYQAIHSAPTYHVAPAGHEAPTYHAAPAAHSATTYHTAPA 393
           + EAP+ H   AAP   E  S     +AP    AP+  + P     P A S      AP 
Sbjct: 523 VPEAPSVHQPPAAPVAPEVPSAPQRPAAPVVPEAPSVPQRPAVPVVPEALSVPQPPVAPV 582

Query: 394 IHATSAIHQVP 426
                ++ Q P
Sbjct: 583 APEVPSVPQPP 593



 Score = 27.5 bits (58), Expect = 1.7
 Identities = 16/68 (23%), Positives = 22/68 (32%)
 Frame = +1

Query: 223 IQEAPTYHAAPAVHEASSYQAIHSAPTYHVAPAGHEAPTYHAAPAAHSATTYHTAPAIHA 402
           + EAP+    P    A    ++   P   V P     P   AAP      +    PA+  
Sbjct: 598 VPEAPSVPQPPVAPVAPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQRPAVPV 657

Query: 403 TSAIHQVP 426
                 VP
Sbjct: 658 VPEAPSVP 665



 Score = 27.5 bits (58), Expect = 1.7
 Identities = 20/70 (28%), Positives = 27/70 (38%), Gaps = 3/70 (4%)
 Frame = +1

Query: 229 EAPTYH---AAPAVHEASSYQAIHSAPTYHVAPAGHEAPTYHAAPAAHSATTYHTAPAIH 399
           E P+     A P V EA S     +AP     P+  + P     P A S      AP + 
Sbjct: 615 EVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVP 674

Query: 400 ATSAIHQVPA 429
              ++ Q PA
Sbjct: 675 EVPSVPQPPA 684



 Score = 26.6 bits (56), Expect = 3.0
 Identities = 17/68 (25%), Positives = 23/68 (33%)
 Frame = +1

Query: 223 IQEAPTYHAAPAVHEASSYQAIHSAPTYHVAPAGHEAPTYHAAPAAHSATTYHTAPAIHA 402
           + EAP+    PAV       ++   P   VAP     P    AP    A +    P    
Sbjct: 553 VPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVPQPPVAPVVPEAPSVPQPPVAPV 612

Query: 403 TSAIHQVP 426
              +  VP
Sbjct: 613 APEVPSVP 620



 Score = 26.2 bits (55), Expect = 4.0
 Identities = 14/61 (22%), Positives = 21/61 (34%)
 Frame = +1

Query: 214 IHTIQEAPTYHAAPAVHEASSYQAIHSAPTYHVAPAGHEAPTYHAAPAAHSATTYHTAPA 393
           +  + EAP+    PA        ++   P   V P     P   AAP      +    PA
Sbjct: 625 VPVVPEAPSVPQPPAAPVVPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQPPA 684

Query: 394 I 396
           +
Sbjct: 685 V 685



 Score = 25.4 bits (53), Expect = 7.0
 Identities = 19/68 (27%), Positives = 23/68 (33%)
 Frame = +1

Query: 223 IQEAPTYHAAPAVHEASSYQAIHSAPTYHVAPAGHEAPTYHAAPAAHSATTYHTAPAIHA 402
           +   P    AP V EA S   +   P   VAP     P   A P    A +    PA   
Sbjct: 586 VPSVPQPPVAPVVPEAPS---VPQPPVAPVAPEVPSVPQRPAVPVVPEAPSVPQPPAAPV 642

Query: 403 TSAIHQVP 426
              +  VP
Sbjct: 643 VPEVPSVP 650



 Score = 25.0 bits (52), Expect = 9.3
 Identities = 15/65 (23%), Positives = 24/65 (36%)
 Frame = +1

Query: 232 APTYHAAPAVHEASSYQAIHSAPTYHVAPAGHEAPTYHAAPAAHSATTYHTAPAIHATSA 411
           AP     P+V +  +   +  AP+    PA    P   + P   +      AP++    A
Sbjct: 610 APVAPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQRPAVPVVPEAPSVPQPPA 669

Query: 412 IHQVP 426
              VP
Sbjct: 670 APVVP 674


>SPAC3G9.14 |sak1||transcriptional repressor
           Sak1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 766

 Score = 30.3 bits (65), Expect = 0.25
 Identities = 15/32 (46%), Positives = 18/32 (56%), Gaps = 2/32 (6%)
 Frame = +2

Query: 434 IQHQLFMKLQLFTFPPHFTP--PQLDMKIIML 523
           +QH LF KL+L   PPH  P  P LD+    L
Sbjct: 342 LQHSLFFKLKLKFLPPHKLPWIPSLDVSSFSL 373


>SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 244

 Score = 30.3 bits (65), Expect = 0.25
 Identities = 16/49 (32%), Positives = 26/49 (53%)
 Frame = +2

Query: 491 PPQLDMKIIMLTNMPTLNTDTLTRLKTLTPATTNLNTRPVMVMSSRVNT 637
           PP   +     T  PT + ++ T   T+ P TT++NT  V+  ++ VNT
Sbjct: 97  PPTTSLNTTTTTAPPTTHVNSTT---TVVPPTTHVNTTTVVPPTTHVNT 142


>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
            hand and WH2 motif |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1794

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 20/64 (31%), Positives = 26/64 (40%)
 Frame = +1

Query: 235  PTYHAAPAVHEASSYQAIHSAPTYHVAPAGHEAPTYHAAPAAHSATTYHTAPAIHATSAI 414
            PT   AP+ H     Q +  AP + VAP   +AP       A S+     AP      A+
Sbjct: 1433 PTNPGAPSNHAP---QVVPPAPMHAVAPVQPKAPGMVTNAPAPSSAPAPPAPVSQLPPAV 1489

Query: 415  HQVP 426
              VP
Sbjct: 1490 PNVP 1493


>SPBC3D6.04c |mad1||mitotic spindle checkpoint protein
           Mad1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 689

 Score = 26.2 bits (55), Expect = 4.0
 Identities = 11/27 (40%), Positives = 19/27 (70%)
 Frame = -2

Query: 523 QHNDLHVQLRRREVRWKREQLELHEQL 443
           Q  +L   L+R+E+ ++REQ+EL  +L
Sbjct: 63  QLENLKNDLKRKELEFEREQIELQRKL 89


>SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1008

 Score = 25.8 bits (54), Expect = 5.3
 Identities = 21/60 (35%), Positives = 34/60 (56%), Gaps = 1/60 (1%)
 Frame = +2

Query: 122 KPSTTRR-PFTTLPCILLPWHRLPLKSMLLQLSTPSKKLLPTMQPQQSTRLLPTKQSTLP 298
           +PST R+ P +T+P  + P  +L   + +   STPSK +LP M P+ + +    + ST P
Sbjct: 446 RPSTPRKVPPSTVPK-MNP--KLQGGNSVTAPSTPSK-VLPAMSPKVAPKFQGGRSSTAP 501


>SPBC16H5.14c ||SPBC21H7.08|short chain dehydrogenase DHRS family
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 286

 Score = 25.8 bits (54), Expect = 5.3
 Identities = 8/28 (28%), Positives = 18/28 (64%)
 Frame = +2

Query: 191 LKSMLLQLSTPSKKLLPTMQPQQSTRLL 274
           +KS + +  TP++ L P ++PQ   +++
Sbjct: 213 IKSTMFEKDTPNRVLAPLLEPQNLAKII 240


>SPBC543.07 |pek1|skh1, mkk1|MAP kinase kinase Pek1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 363

 Score = 25.0 bits (52), Expect = 9.3
 Identities = 10/27 (37%), Positives = 17/27 (62%), Gaps = 1/27 (3%)
 Frame = +2

Query: 458 LQLFTFPPHFTPPQLDMKII-MLTNMP 535
           L  F FPP  +PP + ++++  + NMP
Sbjct: 274 LNRFPFPPEGSPPPMPIELLSYIINMP 300


>SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 509

 Score = 25.0 bits (52), Expect = 9.3
 Identities = 8/18 (44%), Positives = 12/18 (66%)
 Frame = -2

Query: 166 YAGQCSEWASRGGWFDHG 113
           YA +C+EW++ G W   G
Sbjct: 124 YAVRCAEWSTDGRWLISG 141


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.315    0.126    0.387 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,493,392
Number of Sequences: 5004
Number of extensions: 49748
Number of successful extensions: 175
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 147
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 171
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 287744314
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)