Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-1298
(682 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC17D11.07c |rpn2||19S proteasome regulatory subunit Rpn2|Schi... 251 8e-68
SPBP19A11.03c |mts4|rpn1|19S proteasome regulatory subunit Mts4|... 48 1e-06
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 29 0.82
SPBC15C4.02 |||ABC1 kinase family protein|Schizosaccharomyces po... 27 2.5
SPBC651.06 |mug166||sequence orphan|Schizosaccharomyces pombe|ch... 27 3.3
SPCC1620.12c |||GTPase activating protein |Schizosaccharomyces p... 26 4.4
SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces ... 26 5.8
SPAC10F6.01c ||SPAC4C5.05c|sulfite reductase beta subunit |Schiz... 26 5.8
>SPBC17D11.07c |rpn2||19S proteasome regulatory subunit
Rpn2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 965
Score = 251 bits (614), Expect = 8e-68
Identities = 115/222 (51%), Positives = 160/222 (72%), Gaps = 3/222 (1%)
Frame = +2
Query: 5 DMLILKNTKDAI--RVSICHTATVIANAFMHAGTTSDQFLRDNLEWLARATNWAKLTVTA 178
DMLIL TKD++ R S+ H A ANAFM+ GT+SD F RDNL WL++A NW+K T TA
Sbjct: 329 DMLILNRTKDSLEARNSVFHNAVTFANAFMNFGTSSDSFFRDNLSWLSKANNWSKFTATA 388
Query: 179 SLGVIHRGHENESLALMQSYLPKEAGPSSG-YSEGGGLYALGLIHANHGANIIDYLLTQL 355
+LGVIHRG+ N+++ +++ YLP+E PSS YSEGG YA+GLIHANHG + +YL QL
Sbjct: 389 ALGVIHRGYYNQAMNILRPYLPEEDAPSSSTYSEGGAFYAMGLIHANHGRGVTEYLREQL 448
Query: 356 KDAQNEMVRHXXXXXXXXAAMGTHRQDVYEQLKFNLYQDDAVTGEAAGIAMGMVMLGSRH 535
K ++E+V++ M + + +YE +K L+ D+AV G AAGI+MG++MLG+
Sbjct: 449 KHTEDEIVQYGLLLGIGLTGMASRDETLYESVKTILFNDNAVAGSAAGISMGLIMLGTAS 508
Query: 536 AAAIEDMVAYAQETQHEKILRGLAVGIAFTMYGRLEEADALV 661
+AAI++M+ YA ETQHEKI+RGL +GIA +YGR +EAD ++
Sbjct: 509 SAAIDEMLQYAHETQHEKIIRGLGIGIALIVYGRQQEADGII 550
Score = 30.7 bits (66), Expect = 0.20
Identities = 29/108 (26%), Positives = 47/108 (43%), Gaps = 1/108 (0%)
Frame = +2
Query: 62 ATVIANAFMHAGTTSDQFLRDNLEWLARATNW-AKLTVTASLGVIHRGHENESLALMQSY 238
A + A A + GT++ + +RD L N + +LG I N ++ ++
Sbjct: 564 AGMFATALAYCGTSNSKIVRDVLHISVSDVNDDVRRAAVCALGFICFKDPNALISTVE-L 622
Query: 239 LPKEAGPSSGYSEGGGLYALGLIHANHGANIIDYLLTQLKDAQNEMVR 382
L P Y G ALG+ AN G+N LL++L + + VR
Sbjct: 623 LVDSYNPHVRY---GSAIALGIACANSGSNAALDLLSRLVEDATDFVR 667
>SPBP19A11.03c |mts4|rpn1|19S proteasome regulatory subunit
Mts4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 48.0 bits (109), Expect = 1e-06
Identities = 41/211 (19%), Positives = 100/211 (47%), Gaps = 11/211 (5%)
Frame = +2
Query: 62 ATVIANAFMHAGTTSDQFLR---DNLEWLARATNWAKLTVTASLGVIHRGHENESLALMQ 232
A NA ++AG ++D+ + + W+ + ++ TAS+G++ + + L+L+
Sbjct: 370 ANTFVNALVNAGYSNDRLILVDDEKTSWIYKNKESGLISATASIGLLQLWNVDMGLSLLD 429
Query: 233 SYLPKEAGPSSGYSEGGGLYALGL--IHANHGANIIDYLLTQLKDAQNEMVRHXXXXXXX 406
YL S ++ G L +G+ + + A+ +L++ + + +R
Sbjct: 430 KYLYS----SEENTKAGALLGIGVTNVAVRNEADPAMAILSEYLETGSVKLRASAILGLG 485
Query: 407 XAAMGTHRQDVYEQLKFNLYQDDAVTGEA--AGIAMGMVMLGSRH----AAAIEDMVAYA 568
A G +R+D+ + L + D + A +++G++ +G+ + + ++ ++
Sbjct: 486 LAYSGANREDLLDMLSPIVTDTDCPMQLSCLAALSLGLIFVGTCNGDVASTILQTLMERE 545
Query: 569 QETQHEKILRGLAVGIAFTMYGRLEEADALV 661
+ Q+++ R +A+G+A G+ + ADA V
Sbjct: 546 ESAQNDQWGRFMALGLALLFNGKQDLADATV 576
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 28.7 bits (61), Expect = 0.82
Identities = 15/67 (22%), Positives = 33/67 (49%)
Frame = -1
Query: 565 IGNHIFYGSSMSRT*HDHTHSNTCCFSCDSIILIQIKLELFINILSMCAHGS*AQTQAAS 386
+G+ FYG +S + + T NTC S + ++ ++ E+ ++ L + S Q
Sbjct: 784 LGSRKFYGDYLSASKPNGTLWNTCGLSQNDHVIFSMRCEVLVHKLGRTSKPSPRQLVLTK 843
Query: 385 MSYHLIL 365
+ +L++
Sbjct: 844 KNLYLVI 850
>SPBC15C4.02 |||ABC1 kinase family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 594
Score = 27.1 bits (57), Expect = 2.5
Identities = 40/183 (21%), Positives = 72/183 (39%), Gaps = 2/183 (1%)
Frame = +2
Query: 89 HAGTTSDQFLRDNLEWLARATNWAKLTVTASLGVIHRGHENESLALMQSYLPKEAGPSSG 268
HAG +S + R L +++ K+ LG + E LAL + +L
Sbjct: 100 HAGVSSKRAYRVVLAGFLCFSDYKKV-----LGSSYASEEERQLALSECHLRCAERSLKV 154
Query: 269 YSEGGGLYALGLIHANHGANIIDYLLTQLKDAQNEMVRHXXXXXXXXAAMGTHRQDVYEQ 448
+ E GG+Y I + + Y++ K+ N MV+ T +D+
Sbjct: 155 FEENGGIY----IKIGQHLSAMGYVIP--KEWTNTMVK------LQDRCPSTSLKDIDHL 202
Query: 449 LKFNLYQD-DAVTGEAAGIAMGMVMLGSRHAAAIEDM-VAYAQETQHEKILRGLAVGIAF 622
+ + + D E IA+G+ L H A ++D V A + QH + + ++
Sbjct: 203 FRVDTGKGLDETFDEFDPIALGVASLAQVHKARLKDSDVWVAVKVQHPSVSLNSPLDLSM 262
Query: 623 TMY 631
T +
Sbjct: 263 TRW 265
>SPBC651.06 |mug166||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 234
Score = 26.6 bits (56), Expect = 3.3
Identities = 12/47 (25%), Positives = 23/47 (48%)
Frame = +2
Query: 56 HTATVIANAFMHAGTTSDQFLRDNLEWLARATNWAKLTVTASLGVIH 196
HT + ++ GTTS Q +R+ + L + K + ++ V+H
Sbjct: 168 HTPSSASSRASETGTTSPQSMRNQISLLYSKVDQVKTEIASAQAVMH 214
>SPCC1620.12c |||GTPase activating protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 595
Score = 26.2 bits (55), Expect = 4.4
Identities = 15/43 (34%), Positives = 23/43 (53%)
Frame = +2
Query: 422 THRQDVYEQLKFNLYQDDAVTGEAAGIAMGMVMLGSRHAAAIE 550
T+RQ+ E+LK Q+D +T + + GMVML + E
Sbjct: 453 TYRQNNLEELK---NQNDYLTSQITNLEEGMVMLNKENTKLSE 492
>SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1877
Score = 25.8 bits (54), Expect = 5.8
Identities = 13/40 (32%), Positives = 24/40 (60%)
Frame = +2
Query: 11 LILKNTKDAIRVSICHTATVIANAFMHAGTTSDQFLRDNL 130
+++KN +A+ ++IC + +IA GT S+ F R +L
Sbjct: 539 MLVKNGDEALTIAICDSRCLIAR-----GTESNSFKRSDL 573
>SPAC10F6.01c ||SPAC4C5.05c|sulfite reductase beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1473
Score = 25.8 bits (54), Expect = 5.8
Identities = 22/87 (25%), Positives = 39/87 (44%), Gaps = 2/87 (2%)
Frame = +2
Query: 110 QFLRDNLEWLARATNWAKLTVTASLGVIHRGHENESLALM--QSYLPKEAGPSSGYSEGG 283
+F + W+ + WA ++L + +N +L ++ Q Y +EA SS +
Sbjct: 516 EFFTNVSHWIIGSDAWAYDLGNSALHQVLCLEKNVNLLIVDTQPYSTREAVRSSSRKKDI 575
Query: 284 GLYALGLIHANHGANIIDYLLTQLKDA 364
GLYA+ +A + + TQL A
Sbjct: 576 GLYAMNFGNAYVASTALYSSYTQLISA 602
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,662,976
Number of Sequences: 5004
Number of extensions: 49545
Number of successful extensions: 132
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 122
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 129
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 313902888
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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