Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-1270
(816 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 39 7e-04
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 36 0.009
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 35 0.012
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 35 0.016
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 33 0.037
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 30 0.34
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 29 0.79
SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2... 28 1.4
SPCP20C8.01c |||B13958 domain|Schizosaccharomyces pombe|chr 3|||... 27 4.2
SPBC21D10.08c |||sequence orphan|Schizosaccharomyces pombe|chr 2... 27 4.2
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 27 4.2
SPAC1039.05c |||conserved fungal protein|Schizosaccharomyces pom... 26 7.4
SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma... 26 7.4
SPCC320.13c |ark1|aim1, SPCC330.16|aurora-B kinase Ark1|Schizosa... 26 7.4
SPAC1039.03 |||esterase/lipase |Schizosaccharomyces pombe|chr 1|... 26 7.4
SPAC17A5.02c |dbr1||RNA lariat debranching enzyme Dbr1 |Schizosa... 26 7.4
SPBC14C8.10 |mrpl24||mitochondrial ribosomal protein subunit L28... 26 7.4
SPBPB8B6.04c |grt1|SPAPB8B6.04c, SPAPB8B6.04c|transcription fact... 25 9.7
SPBC947.01 |||AAA family ATPase, unknown biological role|Schizos... 25 9.7
SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosacchar... 25 9.7
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 39.1 bits (87), Expect = 7e-04
Identities = 18/60 (30%), Positives = 29/60 (48%)
Frame = +1
Query: 10 MNHKKSRLTDLFRKMDKDNNGLIPRNEFIDGIVNTKFDTSRLEMGAVADLFDRNGSGLID 189
+N+ S L LF + DK G + + + GI KF + + +L+D NG G +D
Sbjct: 574 INNSSSFLRHLFLRFDKSMTGSLSLQDLVSGIAELKFRDVMRNISFIFELYDFNGDGFMD 633
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 35.5 bits (78), Expect = 0.009
Identities = 20/64 (31%), Positives = 32/64 (50%), Gaps = 1/64 (1%)
Frame = +1
Query: 25 SRLTDLFRKMDKDNNGLIPRNEFIDGIVNTKFDT-SRLEMGAVADLFDRNGSGLIDWEEF 201
+ L D+ ++D D NG I EF+ + DT + E+ +FD++G+G I EE
Sbjct: 48 AELQDMINEVDADGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEEL 107
Query: 202 IAAL 213
L
Sbjct: 108 THVL 111
Score = 32.7 bits (71), Expect = 0.064
Identities = 17/60 (28%), Positives = 30/60 (50%)
Frame = +1
Query: 22 KSRLTDLFRKMDKDNNGLIPRNEFIDGIVNTKFDTSRLEMGAVADLFDRNGSGLIDWEEF 201
+ + + F+ DKD NG I E + + S+ E+ + D +G G+I++EEF
Sbjct: 84 EEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEF 143
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 35.1 bits (77), Expect = 0.012
Identities = 22/69 (31%), Positives = 37/69 (53%)
Frame = +1
Query: 7 FMNHKKSRLTDLFRKMDKDNNGLIPRNEFIDGIVNTKFDTSRLEMGAVADLFDRNGSGLI 186
F N + R+ F K+D + +G I RNEF+ I + + + +V D +G G +
Sbjct: 18 FSNEEIERIRKRFIKIDANQSGSIDRNEFL-SIPSVASNPLASRLFSVV---DEDGGGDV 73
Query: 187 DWEEFIAAL 213
D++EFI +L
Sbjct: 74 DFQEFINSL 82
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 34.7 bits (76), Expect = 0.016
Identities = 19/64 (29%), Positives = 30/64 (46%)
Frame = +1
Query: 22 KSRLTDLFRKMDKDNNGLIPRNEFIDGIVNTKFDTSRLEMGAVADLFDRNGSGLIDWEEF 201
+S LF K+D +N G I E + + + D+ E+ + D D G ID +EF
Sbjct: 301 RSNFYQLFSKIDNENKGYIVGGEAVPFFMASHLDSE--ELARIWDTVDTQDRGYIDKDEF 358
Query: 202 IAAL 213
A+
Sbjct: 359 AVAM 362
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 33.5 bits (73), Expect = 0.037
Identities = 16/78 (20%), Positives = 35/78 (44%)
Frame = +1
Query: 1 LKFMNHKKSRLTDLFRKMDKDNNGLIPRNEFIDGIVNTKFDTSRLEMGAVADLFDRNGSG 180
++ ++ + + F+ D D + I +E + F+ + E+ + FD+ G G
Sbjct: 29 VEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKG 88
Query: 181 LIDWEEFIAALRPDWVER 234
+ E+F+ + VER
Sbjct: 89 YLQMEDFVRVMTEKIVER 106
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 30.3 bits (65), Expect = 0.34
Identities = 17/54 (31%), Positives = 25/54 (46%)
Frame = +1
Query: 43 FRKMDKDNNGLIPRNEFIDGIVNTKFDTSRLEMGAVADLFDRNGSGLIDWEEFI 204
FR DKDN+G I +F D + S E+ + D SG D+ +F+
Sbjct: 84 FRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDFV 137
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 29.1 bits (62), Expect = 0.79
Identities = 16/55 (29%), Positives = 26/55 (47%)
Frame = +1
Query: 40 LFRKMDKDNNGLIPRNEFIDGIVNTKFDTSRLEMGAVADLFDRNGSGLIDWEEFI 204
+F D D NG I EFI + T ++ L+D + +GLI ++E +
Sbjct: 68 VFNVFDADKNGYIDFKEFICALSVTSRGELNDKLIWAFQLYDLDNNGLISYDEML 122
Score = 26.2 bits (55), Expect = 5.6
Identities = 11/22 (50%), Positives = 17/22 (77%)
Frame = +1
Query: 148 VADLFDRNGSGLIDWEEFIAAL 213
V ++FD + +G ID++EFI AL
Sbjct: 68 VFNVFDADKNGYIDFKEFICAL 89
Score = 25.8 bits (54), Expect = 7.4
Identities = 21/72 (29%), Positives = 31/72 (43%), Gaps = 12/72 (16%)
Frame = +1
Query: 43 FRKMDKDNNGLIPRNE---FIDGIVN-----TKF----DTSRLEMGAVADLFDRNGSGLI 186
F+ D DNNGLI +E +D I K DT + + ++ D+N G +
Sbjct: 105 FQLYDLDNNGLISYDEMLRIVDAIYKMVGSMVKLPEDEDTPEKRVNKIFNMMDKNKDGQL 164
Query: 187 DWEEFIAALRPD 222
EEF + D
Sbjct: 165 TLEEFCEGSKRD 176
>SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis
Mei2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 750
Score = 28.3 bits (60), Expect = 1.4
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = +2
Query: 137 RWAPWRICSTGTVAASSTGRSSLRRFALTGWNAADPRPMRTKFTT 271
+W+P+ +TG V S TG +RR G NA+ P F +
Sbjct: 501 QWSPFS-SNTGKVFDSPTGSLGMRRSLTVGANASCSNPTNLSFAS 544
>SPCP20C8.01c |||B13958 domain|Schizosaccharomyces pombe|chr
3|||Manual
Length = 247
Score = 26.6 bits (56), Expect = 4.2
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +1
Query: 40 LFRKMDKDNNGLIPRNEFIDGIVNTKFDTSRLEM 141
+F KMD N + R + + +N++FDT + EM
Sbjct: 111 IFSKMDSMQNDMNSRFDAMQNEMNSRFDTVQNEM 144
>SPBC21D10.08c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 277
Score = 26.6 bits (56), Expect = 4.2
Identities = 18/75 (24%), Positives = 34/75 (45%), Gaps = 2/75 (2%)
Frame = +3
Query: 585 NRTHHEGEGKNSS--IGAYVRWSGSGRSRFQIFVECRNTRLAQ**RSGERTWNKIQEAQR 758
N+ G+ KN +G Y + + + Q +EC N R+ + ++ E + I E+Q
Sbjct: 183 NKVQTLGKAKNKKHWLGVYQSFGKASVDQIQKQLECYNVRMMELNKTDENNESAICESQA 242
Query: 759 SEVHFDTGNISTRFS 803
S + + +T S
Sbjct: 243 SSKEDERSDKTTSSS 257
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 26.6 bits (56), Expect = 4.2
Identities = 20/62 (32%), Positives = 23/62 (37%)
Frame = -3
Query: 310 SVGTCTASRVASPSREFCPHRSGVRGVPPSQGEAPQ*TPPSR*GRYRSCRTDPPRRPSPA 131
S GT T++ A P P VP AP PP G S PPR +P
Sbjct: 220 SAGTPTSTS-APPIPPSIPSSRPPERVPSLSAPAPPPIPPPSNGTVSSPPNSPPRPIAPV 278
Query: 130 GM 125
M
Sbjct: 279 SM 280
>SPAC1039.05c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 781
Score = 25.8 bits (54), Expect = 7.4
Identities = 11/45 (24%), Positives = 21/45 (46%)
Frame = +1
Query: 94 IDGIVNTKFDTSRLEMGAVADLFDRNGSGLIDWEEFIAALRPDWV 228
+ G+ + SR +G V ++ NG+ L +W + A W+
Sbjct: 541 LHGLTTIGWILSRENLGIVENIMQNNGTNLKNWRILLKASYKFWL 585
>SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 614
Score = 25.8 bits (54), Expect = 7.4
Identities = 16/64 (25%), Positives = 32/64 (50%)
Frame = +1
Query: 13 NHKKSRLTDLFRKMDKDNNGLIPRNEFIDGIVNTKFDTSRLEMGAVADLFDRNGSGLIDW 192
N + LTD F K+D D G + + I ++K + A+ ++ + + SG ++
Sbjct: 15 NEEILTLTDQFNKLDVDGKGYLDQPTTIKAFEDSKKGSYDEVREAIREV-NVDSSGRVEP 73
Query: 193 EEFI 204
E+F+
Sbjct: 74 EDFV 77
>SPCC320.13c |ark1|aim1, SPCC330.16|aurora-B kinase
Ark1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 355
Score = 25.8 bits (54), Expect = 7.4
Identities = 16/47 (34%), Positives = 24/47 (51%), Gaps = 2/47 (4%)
Frame = -1
Query: 333 SDLEHAELLSARAQHHES--LHLVVNFVRIGRGSAAFHPVRAKRRNE 199
S+L H +L H+ ++L++ F GRG H RAKR +E
Sbjct: 142 SNLRHKNILRLYGHFHDEKRIYLILEFA--GRGELYQHLRRAKRFSE 186
>SPAC1039.03 |||esterase/lipase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 341
Score = 25.8 bits (54), Expect = 7.4
Identities = 13/40 (32%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
Frame = -1
Query: 195 LPVDEAATVPVEQIRHGAHLQPGCVE-FGVDDAIDELVPR 79
LP+ + T PV+ +R+ ++ PG E V+ D +PR
Sbjct: 36 LPIVKTHTYPVDFLRNNGNVMPGQSELLPVESTEDITIPR 75
>SPAC17A5.02c |dbr1||RNA lariat debranching enzyme Dbr1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 466
Score = 25.8 bits (54), Expect = 7.4
Identities = 12/45 (26%), Positives = 20/45 (44%)
Frame = -2
Query: 437 SSSATQPPPTLTMTVERRIRTRRCFWESPNLYFPSPTWNTRNFCR 303
S + Q PP ++ V + ++ + W NL RNFC+
Sbjct: 383 SHTIEQDPPLPSLEVVKTLKRKEEIWVDENLVKKDKLGIPRNFCQ 427
>SPBC14C8.10 |mrpl24||mitochondrial ribosomal protein subunit
L28|Schizosaccharomyces pombe|chr 2|||Manual
Length = 176
Score = 25.8 bits (54), Expect = 7.4
Identities = 19/59 (32%), Positives = 31/59 (52%), Gaps = 4/59 (6%)
Frame = +1
Query: 331 GEGKYRFGDSQKQRLVRILRSTVMVRVG--GGWVALDEFLVKNDPCRAK--GRTNIELR 495
G K+ + + +++ + S V+ + GG LDE+LVK+ P R K G +ELR
Sbjct: 59 GHSKWLYSNVLEEKFHLYVTSRVLRTIDKEGG---LDEYLVKSTPSRLKSLGLRGVELR 114
>SPBPB8B6.04c |grt1|SPAPB8B6.04c, SPAPB8B6.04c|transcription factor
Grt1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 648
Score = 25.4 bits (53), Expect = 9.7
Identities = 11/37 (29%), Positives = 18/37 (48%)
Frame = -2
Query: 527 WDTPSAKINCSRSSMLVRPLALQGSFFTRNSSSATQP 417
W PSA ++ L+ + S+F S+S+T P
Sbjct: 193 WKNPSASVHDKALLYLILSIGSAASYFDLQSNSSTLP 229
>SPBC947.01 |||AAA family ATPase, unknown biological
role|Schizosaccharomyces pombe|chr 2|||Manual
Length = 660
Score = 25.4 bits (53), Expect = 9.7
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = -2
Query: 257 SASVGGPRRSTQSGRSAAMNSSQSMRPLPFLSNRSATAPISSRDVSN 117
SA+ S+ +GRSA MNS+ + ++S T P S VS+
Sbjct: 202 SAAASASALSSDTGRSATMNSTTFPTA---MKSQSTTKPTLSNSVSS 245
>SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1131
Score = 25.4 bits (53), Expect = 9.7
Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 4/49 (8%)
Frame = -2
Query: 221 SGRSAAMNSSQSMRPLPFL----SNRSATAPISSRDVSNLVLTMPSMNS 87
SG + + NSSQ+ P S+ SAT I+ +S V + SMNS
Sbjct: 759 SGYTISSNSSQNSASEPQTAFTSSSSSATPTITQSSISTSVSSQSSMNS 807
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,433,386
Number of Sequences: 5004
Number of extensions: 73978
Number of successful extensions: 245
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 223
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 241
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 398435810
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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