Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-1009
(582 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.09c |paa1||protein phosphatase regulatory subunit Paa1|S... 132 4e-32
SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizos... 37 0.002
SPAC2F3.06c |kap104||karyopherin Kap104|Schizosaccharomyces pomb... 29 0.65
SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom... 27 1.5
SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosacc... 26 3.5
SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr 2|||M... 26 3.5
SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces pombe... 26 3.5
SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb... 25 6.1
SPCC1902.02 |mug72|SPCC663.16c|ketopantoate reductase |Schizosac... 25 6.1
SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit C... 25 6.1
SPBC3B8.09 |||U3 snoRNP-associated protein Utp3 |Schizosaccharom... 25 8.1
SPBC1703.15c |vps33|SPBC2A9.01c|vacuolar sorting protein Vps33|S... 25 8.1
>SPAP8A3.09c |paa1||protein phosphatase regulatory subunit
Paa1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 590
Score = 132 bits (319), Expect = 4e-32
Identities = 68/156 (43%), Positives = 100/156 (64%)
Frame = +2
Query: 23 LLAPEDMEQHVMPTVRARAGDTSWRVRYMVADKFVELQQAVGPELARTDLAQIFQALLKD 202
L + ++ +++ +R+ D+SWR RYMVA FV+L + VGP L + +L + F L+KD
Sbjct: 236 LKSDSEIRHYLLQPLRSFVSDSSWRTRYMVAANFVKLAKVVGPSLIKDELIKPFVLLMKD 295
Query: 203 SEAEVRAAAAGKVKDFCMNLDKAHQEHIIMTMILPQIKDLVCDANQHVKSGLAFVIMGLS 382
+E EVR A A ++ FC LDK I++ I+P I++L+ D QHV++ L I L+
Sbjct: 296 TEQEVRRAIATQIPGFCELLDK----RIVLEEIIPVIQELINDPAQHVRAALGMNIGALA 351
Query: 383 PIVGRQNTIEHFLPLFLTQLKDECSEVRLNIISNLE 490
P +G++ T E+ LP+FL LKDE EVRLNIIS LE
Sbjct: 352 PQLGKEKTTEYLLPMFLELLKDENPEVRLNIISKLE 387
Score = 42.7 bits (96), Expect = 4e-05
Identities = 33/155 (21%), Positives = 65/155 (41%)
Frame = +2
Query: 14 VASLLAPEDMEQHVMPTVRARAGDTSWRVRYMVADKFVELQQAVGPELARTDLAQIFQAL 193
+A ++ P ++ ++ DT VR +A + + + + ++ + Q L
Sbjct: 272 LAKVVGPSLIKDELIKPFVLLMKDTEQEVRRAIATQIPGFCELLDKRIVLEEIIPVIQEL 331
Query: 194 LKDSEAEVRAAAAGKVKDFCMNLDKAHQEHIIMTMILPQIKDLVCDANQHVKSGLAFVIM 373
+ D VRAA + L K ++ M L +L+ D N V+ + +
Sbjct: 332 INDPAQHVRAALGMNIGALAPQLGKEKTTEYLLPMFL----ELLKDENPEVRLNIISKLE 387
Query: 374 GLSPIVGRQNTIEHFLPLFLTQLKDECSEVRLNII 478
++ +VG + + LP +T +D+ VRL II
Sbjct: 388 VVNKVVGIELLSQSLLPAIVTLAEDKQWRVRLAII 422
Score = 29.1 bits (62), Expect = 0.50
Identities = 30/122 (24%), Positives = 50/122 (40%), Gaps = 1/122 (0%)
Frame = +2
Query: 107 MVADKFVELQQAVGPELARTDLAQIFQALLKD-SEAEVRAAAAGKVKDFCMNLDKAHQEH 283
+V DK V+ V L++ L Q F L++ S AE + A +C ++ +
Sbjct: 103 VVRDKAVDSLNKVCICLSQEQLEQYFVPLVQRLSTAEWFTSRASSAGLYCAAYSQS-ENP 161
Query: 284 IIMTMILPQIKDLVCDANQHVKSGLAFVIMGLSPIVGRQNTIEHFLPLFLTQLKDECSEV 463
+ + L D V+ A +V +Q I+ F+PLF + D+ V
Sbjct: 162 AVKVSLRQSFSHLCHDEAPMVRRPAATNCAKFVFLVTKQEAIDEFIPLFNSLSNDDQDSV 221
Query: 464 RL 469
RL
Sbjct: 222 RL 223
Score = 27.9 bits (59), Expect = 1.1
Identities = 30/137 (21%), Positives = 56/137 (40%)
Frame = +2
Query: 83 DTSWRVRYMVADKFVELQQAVGPELARTDLAQIFQALLKDSEAEVRAAAAGKVKDFCMNL 262
D + VR A + V + A + +F +L D + VR + + L
Sbjct: 177 DEAPMVRRPAATNCAKFVFLVTKQEAIDEFIPLFNSLSNDDQDSVRLLSFDIMVSLAEVL 236
Query: 263 DKAHQEHIIMTMILPQIKDLVCDANQHVKSGLAFVIMGLSPIVGRQNTIEHFLPLFLTQL 442
K+ E I +L ++ V D++ + +A + L+ +VG + + F+ +
Sbjct: 237 -KSDSE--IRHYLLQPLRSFVSDSSWRTRYMVAANFVKLAKVVGPSLIKDELIKPFVLLM 293
Query: 443 KDECSEVRLNIISNLEG 493
KD EVR I + + G
Sbjct: 294 KDTEQEVRRAIATQIPG 310
>SPBC119.07 |ppk19||serine/threonine protein kinase
Ppk19|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1706
Score = 37.1 bits (82), Expect = 0.002
Identities = 25/101 (24%), Positives = 46/101 (45%)
Frame = +2
Query: 191 LLKDSEAEVRAAAAGKVKDFCMNLDKAHQEHIIMTMILPQIKDLVCDANQHVKSGLAFVI 370
LL DS + VR + + C+ KA +I++ ++ + D + ++ I
Sbjct: 600 LLADSSSIVRRSLLNALAPLCVFFGKAKSNDLILSHLITYLND----TDWMLRCAFFESI 655
Query: 371 MGLSPIVGRQNTIEHFLPLFLTQLKDECSEVRLNIISNLEG 493
GLS +G ++ E+ LPL L L D V +++ + G
Sbjct: 656 TGLSIFIGPRSVDEYILPLMLQALVDPEPAVLESVLGSFSG 696
Score = 25.0 bits (52), Expect = 8.1
Identities = 21/96 (21%), Positives = 36/96 (37%)
Frame = +2
Query: 83 DTSWRVRYMVADKFVELQQAVGPELARTDLAQIFQALLKDSEAEVRAAAAGKVKDFCMNL 262
DT W +R + L +GP + + L D E V + G F +
Sbjct: 642 DTDWMLRCAFFESITGLSIFIGPRSVDEYILPLMLQALVDPEPAVLESVLG---SFSGLI 698
Query: 263 DKAHQEHIIMTMILPQIKDLVCDANQHVKSGLAFVI 370
+ E +++ IL + LV N +++ VI
Sbjct: 699 ELHLFEKLVVVDILQLVLPLVAVPNAYIRRAALSVI 734
>SPAC2F3.06c |kap104||karyopherin Kap104|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 910
Score = 28.7 bits (61), Expect = 0.65
Identities = 23/68 (33%), Positives = 28/68 (41%), Gaps = 2/68 (2%)
Frame = +2
Query: 59 PTVRARAGDTSWRVRYMVADKFVELQQAVGPELARTD--LAQIFQALLKDSEAEVRAAAA 232
PTV D +V+ L V P LA+ D L QI KD EVR +A
Sbjct: 651 PTVDVPDRDFLVTTLDLVSGIIQALGSQVSPLLAQADPPLGQIIGICAKDEVPEVRQSAY 710
Query: 233 GKVKDFCM 256
+ D CM
Sbjct: 711 ALLGDMCM 718
>SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1067
Score = 27.5 bits (58), Expect = 1.5
Identities = 13/29 (44%), Positives = 18/29 (62%)
Frame = +2
Query: 167 DLAQIFQALLKDSEAEVRAAAAGKVKDFC 253
D+ +F A L+DSE EVR+ AA + C
Sbjct: 894 DVFSLFMAALEDSEGEVRSNAAYSMGLLC 922
>SPCC132.04c |||NAD-dependent glutamate dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1106
Score = 26.2 bits (55), Expect = 3.5
Identities = 9/42 (21%), Positives = 21/42 (50%)
Frame = -1
Query: 420 RKCSIVFCLPTMGLRPIMTKARPDLTCWLASHTKSLICGKIM 295
R+ S+++CLP+ +P+ + + +H + C +M
Sbjct: 387 REASLLYCLPSTDFQPLFVSEKLSIQEVTYAHCVRIFCEHVM 428
>SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 685
Score = 26.2 bits (55), Expect = 3.5
Identities = 13/33 (39%), Positives = 17/33 (51%)
Frame = +2
Query: 287 IMTMILPQIKDLVCDANQHVKSGLAFVIMGLSP 385
I T+ + I+ L D H KSG MGL+P
Sbjct: 9 IDTLAINTIRTLAVDTTAHAKSGHPGAPMGLAP 41
>SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 570
Score = 26.2 bits (55), Expect = 3.5
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = -3
Query: 322 QVLDLWQDHGHDNVFLMSFIQVHTEVLDLP 233
++LDL++D ++ ++VH VLD P
Sbjct: 525 ELLDLFKDEEFGKADVIQLVEVHMPVLDAP 554
>SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2685
Score = 25.4 bits (53), Expect = 6.1
Identities = 13/31 (41%), Positives = 19/31 (61%)
Frame = -3
Query: 268 FIQVHTEVLDLPSSCGSNLSLRVLEQCLKDL 176
FIQ+HT+ D+P+ L+L + LKDL
Sbjct: 814 FIQIHTKNSDVPTLWDRFLALGNVRYQLKDL 844
>SPCC1902.02 |mug72|SPCC663.16c|ketopantoate reductase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 574
Score = 25.4 bits (53), Expect = 6.1
Identities = 11/42 (26%), Positives = 21/42 (50%)
Frame = +2
Query: 299 ILPQIKDLVCDANQHVKSGLAFVIMGLSPIVGRQNTIEHFLP 424
ILP + +L + V G +++ + IVG + ++H P
Sbjct: 91 ILPSVYNLDTAIKEVVTPGHTCIVLNTTGIVGAEKELQHAFP 132
>SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit
Cct2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 527
Score = 25.4 bits (53), Expect = 6.1
Identities = 16/51 (31%), Positives = 25/51 (49%)
Frame = -3
Query: 343 VLVGITHQVLDLWQDHGHDNVFLMSFIQVHTEVLDLPSSCGSNLSLRVLEQ 191
VL G THQ+LD + HD + ++S + V L C L + +E+
Sbjct: 370 VLRGATHQLLDESERAIHDALAVLSQTVAESRV-TLGGGCAEMLMAKAVEE 419
>SPBC3B8.09 |||U3 snoRNP-associated protein Utp3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 597
Score = 25.0 bits (52), Expect = 8.1
Identities = 10/21 (47%), Positives = 15/21 (71%)
Frame = +2
Query: 128 ELQQAVGPELARTDLAQIFQA 190
EL Q + P+L RT+L +I +A
Sbjct: 191 ELVQQISPDLPRTELLKILEA 211
>SPBC1703.15c |vps33|SPBC2A9.01c|vacuolar sorting protein
Vps33|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 25.0 bits (52), Expect = 8.1
Identities = 16/61 (26%), Positives = 27/61 (44%)
Frame = +2
Query: 170 LAQIFQALLKDSEAEVRAAAAGKVKDFCMNLDKAHQEHIIMTMILPQIKDLVCDANQHVK 349
L++I + L D E +A +++DF L EH + + + LV QH K
Sbjct: 294 LSKIARKLSSDFEGRRQAKTVNQIRDFVSKLGSLQSEHTSLNIHTGLAETLV----QHTK 349
Query: 350 S 352
+
Sbjct: 350 N 350
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,516,867
Number of Sequences: 5004
Number of extensions: 51558
Number of successful extensions: 145
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 134
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 142
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 250133048
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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