Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ceN-0886
         (702 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC285.08 |ret2||coatomer delta subunit Ret2 |Schizosaccharomyc...   171   6e-44
SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosacc...    38   0.001
SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosac...    33   0.030
SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosacc...    33   0.030
SPAC1B1.01 |||transcription factor Rdp1|Schizosaccharomyces pomb...    27   2.6  
SPAP27G11.06c |||AP-1 adaptor complex subunit |Schizosaccharomyc...    26   4.5  
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos...    26   6.0  
SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces ...    25   7.9  
SPAC328.06 |ubp2||ubiquitin C-terminal hydrolase Ubp2|Schizosacc...    25   7.9  
SPBC14C8.01c |cut2|SPBC1815.02c|securin|Schizosaccharomyces pomb...    25   7.9  
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc...    25   7.9  

>SPCC285.08 |ret2||coatomer delta subunit Ret2 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 240

 Score =  171 bits (417), Expect = 6e-44
 Identities = 79/176 (44%), Positives = 124/176 (70%)
 Frame = +3

Query: 48  MVLIAATVCTKSGKALVSRQFVEMTKARIEGLLAAFPKLMTGGRQHTFVETESVRYVYQP 227
           MV++A ++  + GKA++SRQF EM++ R+E LL++FP L++   Q+T VE+++VR+VYQP
Sbjct: 1   MVVLAVSIVNRGGKAIISRQFREMSRVRVESLLSSFPALVSEKSQNTTVESDNVRFVYQP 60

Query: 228 LDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAFNLLFAFDEIVALG 407
           LD+LY++LIT   SNIL+D++TL L S+VV   C  L E E+L  AF +  AFDE  +LG
Sbjct: 61  LDELYIVLITNLQSNILQDIDTLHLLSQVVTSICSSLEEREILEYAFEIFTAFDEATSLG 120

Query: 408 YRESVNLAQVRSFVEMDSHEEKIYQAVRQTQXXXXXXXXXXXXXXLQRERLEAAKR 575
           YR++V+L Q+++++EM+SHEEKI + V + +              L+ ++ EAA+R
Sbjct: 121 YRDNVSLTQIKTYLEMESHEEKIQEIVSRNKEIEATEERKRRIKQLELQKKEAARR 176


>SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 426

 Score = 38.3 bits (85), Expect = 0.001
 Identities = 29/140 (20%), Positives = 63/140 (45%), Gaps = 2/140 (1%)
 Frame = +3

Query: 60  AATVCTKSGKALVSRQF-VEMTKARIEGLLAAFPKLMTG-GRQHTFVETESVRYVYQPLD 233
           A  V    GK ++SR +  ++  + +E  L    ++    G     +  E + Y+Y   +
Sbjct: 4   AIFVLNLKGKVIISRDYRADIPMSVVEKFLPLKSEVEEEQGFSTPCLTHEGINYIYIHHN 63

Query: 234 KLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAFNLLFAFDEIVALGYR 413
            +Y+L ++   S+ +E L  LR  + V  +Y  +L E  + +    +    DEI+  G+ 
Sbjct: 64  DVYLLALSKMNSDAMEMLVFLRKMADVFIDYFKELQEESIRDNFVLVYELLDEIMDFGFP 123

Query: 414 ESVNLAQVRSFVEMDSHEEK 473
           ++     ++ ++   S+  K
Sbjct: 124 QTTETKILQEYITQTSNTVK 143


>SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 446

 Score = 33.5 bits (73), Expect = 0.030
 Identities = 20/76 (26%), Positives = 34/76 (44%)
 Frame = +3

Query: 180 QHTFVETESVRYVYQPLDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLN 359
           +H  V   S  Y+Y   + LY++ IT    N++  LE L    + +  Y  +L E  V +
Sbjct: 43  RHPIVSIGSSTYIYTKHEDLYVVAITKGNPNVMIVLEFLESLIQDLTHYFGKLNENTVKD 102

Query: 360 QAFNLLFAFDEIVALG 407
               +    DE++  G
Sbjct: 103 NVSFIFELLDEMIDYG 118


>SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 143

 Score = 33.5 bits (73), Expect = 0.030
 Identities = 28/128 (21%), Positives = 51/128 (39%), Gaps = 3/128 (2%)
 Frame = +3

Query: 78  KSGKALVSRQFVEMTKARIEGLLAAFPKLMTGGRQH---TFVETESVRYVYQPLDKLYML 248
           + GK  +S+ +V         L A   +L++   Q     F+E E+ + VY+    LY  
Sbjct: 10  RHGKNRLSKYYVPFDDDEKVRLKARIHQLISQRNQKFQANFLEWENSKLVYRRYAGLYFC 69

Query: 249 LITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAFNLLFAFDEIVALGYRESVNL 428
                  N L  LE +  F  ++  +   + E +++   + +    DEI+  G     N 
Sbjct: 70  FCVDSTDNDLAILEMIHFFVEILDSFFGNVCELDLIFNFYKVSAILDEIILGGEIGESNK 129

Query: 429 AQVRSFVE 452
             V   +E
Sbjct: 130 KSVLERIE 137


>SPAC1B1.01 |||transcription factor Rdp1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 478

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 10/25 (40%), Positives = 16/25 (64%)
 Frame = +1

Query: 544 CSVRDWRRPNVASRRAHRCPSAVAL 618
           C  ++WRRP   +  A +CPS +A+
Sbjct: 189 CGRQNWRRPVNFASHAKQCPSFLAM 213


>SPAP27G11.06c |||AP-1 adaptor complex subunit |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 162

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 18/87 (20%), Positives = 37/87 (42%)
 Frame = +3

Query: 189 FVETESVRYVYQPLDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAF 368
           FVE +  + VY+    L+ +    +  N L  LE +  F   + +Y   + E +++    
Sbjct: 50  FVEYKGEKIVYRRYASLFFVCGIEQDDNELIILEVIHKFVECLDKYFGNVCELDLIFNFE 109

Query: 369 NLLFAFDEIVALGYRESVNLAQVRSFV 449
              +  +E++  G  +  +   V S V
Sbjct: 110 KAYYVMEELLLAGELQESSKTNVLSAV 136


>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
           Plc1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 899

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 13/34 (38%), Positives = 19/34 (55%)
 Frame = -3

Query: 337 SCTQYSGTTLLKSLNVSRSSRMLLAFVVISNIYN 236
           SC      + L S+N + +SR LLA +V  +I N
Sbjct: 134 SCDSNENLSTLSSVNQNFNSRQLLATIVPESIQN 167


>SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces
            pombe|chr 3|||Manual
          Length = 2344

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 8/28 (28%), Positives = 15/28 (53%)
 Frame = -3

Query: 187  VCCRPPVISLGKAASRPSMRAFVISTNW 104
            +CC P  +++ +   +PS R+F     W
Sbjct: 2128 LCCDPSRLNIEQELQKPSGRSFACKKRW 2155


>SPAC328.06 |ubp2||ubiquitin C-terminal hydrolase
           Ubp2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1141

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 14/36 (38%), Positives = 26/36 (72%), Gaps = 1/36 (2%)
 Frame = +3

Query: 171 GGRQHTFVE-TESVRYVYQPLDKLYMLLITTKASNI 275
           GGR  T +E   ++++ Y+ L KL++ LIT+K+S++
Sbjct: 664 GGRIVTRIEFLRALQFTYE-LRKLFIELITSKSSSV 698


>SPBC14C8.01c |cut2|SPBC1815.02c|securin|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 301

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 21/64 (32%), Positives = 31/64 (48%), Gaps = 4/64 (6%)
 Frame = -1

Query: 684 PVSLESSRQ*IQ-METTRTSSSYQSHCRRTSV---SAAAGHVWPPPISHAATP*RVRACG 517
           P +L++S Q  + +E    S    S  R ++V     A+G   P P+SH  T  RV A G
Sbjct: 213 PATLKASDQQRKVLEKPSVSKQSSSRTRLSTVYRTKLASGKSIPRPLSHKLTRPRVTASG 272

Query: 516 SLPR 505
           +  R
Sbjct: 273 NSRR 276


>SPAC29B12.07 |sec16||multidomain vesicle coat component
            Sec16|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1995

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 26/92 (28%), Positives = 41/92 (44%), Gaps = 1/92 (1%)
 Frame = +3

Query: 228  LDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTET-EVLNQAFNLLFAFDEIVAL 404
            L  L MLL  +    I+E L  + L S  +PE C   T   E++N   N       IVA 
Sbjct: 1197 LQCLKMLLEVSDRKLIVEKLRPILLPSFEIPEPCNTATSVQELINPEINQ--DDSPIVAS 1254

Query: 405  GYRESVNLAQVRSFVEMDSHEEKIYQAVRQTQ 500
             Y  +  L +   ++   + +E +  A++Q Q
Sbjct: 1255 RYCTTSFLHRFYEYLLSGNKDEALTYALQQKQ 1286


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,796,874
Number of Sequences: 5004
Number of extensions: 54603
Number of successful extensions: 141
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 138
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 141
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 325165428
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)