Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-0884
(711 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 46 2e-05
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 46 3e-05
At4g27670.1 68417.m03979 25.3 kDa small heat shock protein, chlo... 45 6e-05
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 44 1e-04
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 44 1e-04
At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein (HS... 44 1e-04
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 44 1e-04
At1g52560.1 68414.m05933 26.5 kDa class I small heat shock prote... 38 0.007
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 33 0.19
At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock pro... 33 0.25
At3g12020.1 68416.m01490 kinesin motor protein-related similar t... 30 1.7
At3g15250.1 68416.m01926 expressed protein ; expression supporte... 29 2.3
At1g53770.1 68414.m06119 expressed protein 28 5.3
At5g55300.1 68418.m06891 DNA topoisomerase I identical to Swiss-... 28 7.0
At1g31440.1 68414.m03850 SH3 domain-containing protein 1 (SH3P1)... 28 7.0
At5g50630.1 68418.m06272 nodulin family protein similar to nodul... 27 9.3
At5g50520.1 68418.m06257 nodulin family protein similar to nodul... 27 9.3
At1g35160.1 68414.m04360 14-3-3 protein GF14 phi (GRF4) identica... 27 9.3
At1g27680.1 68414.m03383 glucose-1-phosphate adenylyltransferase... 27 9.3
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 46.4 bits (105), Expect = 2e-05
Identities = 37/117 (31%), Positives = 57/117 (48%), Gaps = 8/117 (6%)
Frame = +2
Query: 206 AIANRDVGSTITSNKDKFQVNLDVQHFSPEEISVKTADGYVI-VEGKH----EERQDEHG 370
AI N V T+ F+ D+ EE+ V+ D V+ + G+ EE+QD
Sbjct: 44 AITNARVDWKETAEAHVFKA--DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWH 101
Query: 371 YISR---QFTRRYALPENCNPDTVESRLSSDGVLTVIAPRTPAATKNERAVPITQTG 532
+ R QF+R++ LPEN D V++ + +GVLTV P+ A K + I +G
Sbjct: 102 RVERSSGQFSRKFKLPENVKMDQVKASM-ENGVLTVTVPKVEEAKKKAQVKSIDISG 157
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 45.6 bits (103), Expect = 3e-05
Identities = 42/163 (25%), Positives = 75/163 (46%), Gaps = 8/163 (4%)
Frame = +2
Query: 59 MSLLPFILGYQPNRLRDQDFGLALTPDDLLTAVITPTMSRDYYRPWKQMAIANRDVGSTI 238
MSL+P I G + + D +L D +TP+ + A N V
Sbjct: 1 MSLIPSIFGGRRTNVFDP---FSLDVFDPFEGFLTPSGLANA-PAMDVAAFTNAKVDWRE 56
Query: 239 TSNKDKFQVNLDVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTR 394
T F+ D+ EE+ V+ DG ++ + G+ +EE+ D+ + R +FTR
Sbjct: 57 TPEAHVFKA--DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTR 114
Query: 395 RYALPENCNPDTVESRLSSDGVLTVIAPRTPAATKNERAVPIT 523
R+ LPEN + +++ + +GVL+V P+ P +++ I+
Sbjct: 115 RFRLPENAKMEEIKASM-ENGVLSVTVPKVPEKKPEVKSIDIS 156
>At4g27670.1 68417.m03979 25.3 kDa small heat shock protein,
chloroplast precursor (HSP25.3-P) identical to small
heat shock protein, chloroplast precursor SP:P31170 from
[Arabidopsis thaliana]; identified in Scharf, K-D., et
al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 227
Score = 44.8 bits (101), Expect = 6e-05
Identities = 20/91 (21%), Positives = 45/91 (49%), Gaps = 3/91 (3%)
Frame = +2
Query: 236 ITSNKDKFQVNLDVQHFSPEEISVKTADGYVIVEGKHEERQDEHGYISRQ---FTRRYAL 406
I + + ++ D+ S E++ + D ++++G+ ++ + + R + R L
Sbjct: 131 IKEEEHEIKMRFDMPGLSKEDVKISVEDNVLVIKGEQKKEDSDDSWSGRSVSSYGTRLQL 190
Query: 407 PENCNPDTVESRLSSDGVLTVIAPRTPAATK 499
P+NC D +++ L +GVL + P+T K
Sbjct: 191 PDNCEKDKIKAEL-KNGVLFITIPKTKVERK 220
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 44.0 bits (99), Expect = 1e-04
Identities = 27/92 (29%), Positives = 50/92 (54%), Gaps = 8/92 (8%)
Frame = +2
Query: 272 DVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPD 427
D+ EE+ V+ D V+ + G+ +EE+ D+ + R +F RR+ LPEN +
Sbjct: 68 DLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKME 127
Query: 428 TVESRLSSDGVLTVIAPRTPAATKNERAVPIT 523
V++ + +GVLTV+ P+ P +++ I+
Sbjct: 128 EVKATM-ENGVLTVVVPKAPEKKPQVKSIDIS 158
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 44.0 bits (99), Expect = 1e-04
Identities = 34/114 (29%), Positives = 57/114 (50%), Gaps = 8/114 (7%)
Frame = +2
Query: 206 AIANRDVGSTITSNKDKFQVNLDVQHFSPEEISVKTADGYVI-VEG-KHEERQDEHGYIS 379
AI N V T F+ D+ EE+ V+ + V+ + G +H E++D++
Sbjct: 42 AIVNARVDWRETPEAHVFKA--DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWH 99
Query: 380 R------QFTRRYALPENCNPDTVESRLSSDGVLTVIAPRTPAATKNERAVPIT 523
R QFTRR+ LPEN D V++ + +GVLTV P+ + +++ I+
Sbjct: 100 RVERSSGQFTRRFRLPENVKMDQVKAAM-ENGVLTVTVPKAETKKADVKSIQIS 152
>At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein
(HSP17.6A-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]
Length = 155
Score = 44.0 bits (99), Expect = 1e-04
Identities = 36/117 (30%), Positives = 56/117 (47%), Gaps = 8/117 (6%)
Frame = +2
Query: 206 AIANRDVGSTITSNKDKFQVNLDVQHFSPEEISVKTADGYVI-VEGKH----EERQDEHG 370
AIAN V T+ F+ D+ EE+ V+ D V+ + G+ EE+QD
Sbjct: 42 AIANARVDWKETAEAHVFKA--DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWH 99
Query: 371 YISRQ---FTRRYALPENCNPDTVESRLSSDGVLTVIAPRTPAATKNERAVPITQTG 532
+ R F+R++ LPEN D V++ + +GVLTV P+ K + I +G
Sbjct: 100 RVERSSGGFSRKFRLPENVKMDQVKASM-ENGVLTVTVPKVETNKKKAQVKSIDISG 155
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 43.6 bits (98), Expect = 1e-04
Identities = 44/164 (26%), Positives = 75/164 (45%), Gaps = 9/164 (5%)
Frame = +2
Query: 59 MSLLPFILGYQPNRLRDQDFGLALTPDDLLTAVITPTMSRDYYRPWKQMA-IANRDVGST 235
MSL+P G + + D +L D +TP ++ P K +A N V
Sbjct: 1 MSLVPSFFGGRRTNVFDP---FSLDVWDPFEGFLTPGLTN---APAKDVAAFTNAKVDWR 54
Query: 236 ITSNKDKFQVNLDVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFT 391
T F+ DV EE+ V+ DG ++ + G+ +EE+ D + R +F
Sbjct: 55 ETPEAHVFKA--DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFM 112
Query: 392 RRYALPENCNPDTVESRLSSDGVLTVIAPRTPAATKNERAVPIT 523
RR+ LPEN + V++ + +GVL+V P+ + ++V I+
Sbjct: 113 RRFRLPENAKVEEVKASM-ENGVLSVTVPKVQESKPEVKSVDIS 155
>At1g52560.1 68414.m05933 26.5 kDa class I small heat shock
protein-like (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family; identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 232
Score = 37.9 bits (84), Expect = 0.007
Identities = 21/104 (20%), Positives = 51/104 (49%), Gaps = 9/104 (8%)
Frame = +2
Query: 236 ITSNKDKFQVNLDVQHFSPEEISVKTADGYVIVEGKHEERQD-----EHGYISRQ----F 388
+ D +++ +V + E++ + DG + ++G H+ ++ E Y S + +
Sbjct: 129 VKEQDDCYKLRYEVPGLTKEDVKITVNDGILTIKGDHKAEEEKGSPEEDEYWSSKSYGYY 188
Query: 389 TRRYALPENCNPDTVESRLSSDGVLTVIAPRTPAATKNERAVPI 520
+LP++ + +++ L + GVL ++ PRT KN + + +
Sbjct: 189 NTSLSLPDDAKVEDIKAELKN-GVLNLVIPRTEKPKKNVQEISV 231
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 33.1 bits (72), Expect = 0.19
Identities = 28/103 (27%), Positives = 40/103 (38%), Gaps = 4/103 (3%)
Frame = +2
Query: 293 EEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTVI 472
+EI D Y EG EE ++ R + L E+C + VES D
Sbjct: 48 DEIDSAYGDFYEYEEGVPEEESRKNNRYERVVNYEFELAEDCEDENVESEDDDDDDDDDD 107
Query: 473 APRTPAATKNERAVPIT----QTGPVRKEIKEPTAEAESNETK 589
R KN +PI+ ++ R EP E+E N T+
Sbjct: 108 DDRHSRMLKNVTELPISAFQGESKNKRVVFTEPYPESEFNPTR 150
>At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock
protein (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family: identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 134
Score = 32.7 bits (71), Expect = 0.25
Identities = 20/81 (24%), Positives = 36/81 (44%)
Frame = +2
Query: 239 TSNKDKFQVNLDVQHFSPEEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENC 418
+ + D ++D+ EEI V+ D ++ + F R++ LPE+
Sbjct: 31 SQSPDSHTFSVDLPGLRKEEIKVEIEDSIYLIIRTEATPMSPPDQPLKTFKRKFRLPESI 90
Query: 419 NPDTVESRLSSDGVLTVIAPR 481
+ + + DGVLTVI P+
Sbjct: 91 DMIGISAGYE-DGVLTVIVPK 110
>At3g12020.1 68416.m01490 kinesin motor protein-related similar to
putative kinesin heavy chain GB:AAD23684 GI:4567271 from
[Arabidopsis thaliana]
Length = 1030
Score = 29.9 bits (64), Expect = 1.7
Identities = 24/95 (25%), Positives = 43/95 (45%), Gaps = 6/95 (6%)
Frame = +2
Query: 317 DGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTVI----APRT 484
D YV VEG HE R ++ Y + TR++ L P + S+ +V+ P T
Sbjct: 524 DLYVSVEGNHEIR--DNAYREEKKTRKHGLLNWLKPKKRDHSSSASDQSSVVKSNSTPST 581
Query: 485 P--AATKNERAVPITQTGPVRKEIKEPTAEAESNE 583
P + +++ P+ +++ EP + E+ E
Sbjct: 582 PQGGGSHLHTESRLSEGSPLMEQLSEPREDREALE 616
>At3g15250.1 68416.m01926 expressed protein ; expression supported
by MPSS
Length = 217
Score = 29.5 bits (63), Expect = 2.3
Identities = 16/53 (30%), Positives = 25/53 (47%)
Frame = +2
Query: 416 CNPDTVESRLSSDGVLTVIAPRTPAATKNERAVPITQTGPVRKEIKEPTAEAE 574
CN S S+ T +PRT +T N+ PI Q P+ ++K P + +
Sbjct: 75 CNSTNSSSSFGSNNSSTSQSPRTSYSTSNKNNSPINQE-PLDLKLKPPISRCD 126
>At1g53770.1 68414.m06119 expressed protein
Length = 563
Score = 28.3 bits (60), Expect = 5.3
Identities = 22/63 (34%), Positives = 28/63 (44%)
Frame = -3
Query: 571 SFRSGLLNLLPDRAGLSDGNSSLVLRGSRSPGSDHGQHTVRGQPRFDSVRVAVFRQSVTP 392
+FRS N L R SD + +LR S HTV D V+ AVFRQ
Sbjct: 102 AFRS---NTLTGRVHESDLQALYLLRKQESDLFSIWNHTVSNLSTIDDVKSAVFRQISLN 158
Query: 391 REL 383
R++
Sbjct: 159 RQI 161
>At5g55300.1 68418.m06891 DNA topoisomerase I identical to
Swiss-Prot:P30181 DNA topoisomerase I [Arabidopsis
thaliana]
Length = 916
Score = 27.9 bits (59), Expect = 7.0
Identities = 10/31 (32%), Positives = 17/31 (54%)
Frame = +2
Query: 494 TKNERAVPITQTGPVRKEIKEPTAEAESNET 586
++ +AVP T+ P+R + P SN+T
Sbjct: 43 SQRSKAVPTTKVSPMRSPVTSPNGTTPSNKT 73
>At1g31440.1 68414.m03850 SH3 domain-containing protein 1 (SH3P1)
nearly identical to SH3 domain-containing protein 1
[Arabidopsis thaliana] GI:16974676; contains Pfam
profile PF00018: SH3 domain
Length = 439
Score = 27.9 bits (59), Expect = 7.0
Identities = 16/38 (42%), Positives = 17/38 (44%)
Frame = +1
Query: 115 LRTGAYSGRSPDGRHHANDVQRLLPSVEADGDRQ*RRR 228
+RT YS D RH N RL VEA RRR
Sbjct: 136 IRTMIYSAPLEDARHLVNHYDRLRQEVEAQATDVLRRR 173
>At5g50630.1 68418.m06272 nodulin family protein similar to
nodulin-like protein [Arabidopsis thaliana] GI:3329368,
nodule-specific protein Nlj70 [Lotus japonicus]
GI:3329366
Length = 540
Score = 27.5 bits (58), Expect = 9.3
Identities = 14/57 (24%), Positives = 27/57 (47%)
Frame = +2
Query: 527 TGPVRKEIKEPTAEAESNETKQ*LSERCESKKCYSVC*FYLHSSCVLVRLIALSYLY 697
+G + I + A ++ T + S C CYSV + C++ +++LS +Y
Sbjct: 468 SGVIASNIYDYYARKQAGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVY 524
>At5g50520.1 68418.m06257 nodulin family protein similar to
nodulin-like protein [Arabidopsis thaliana] GI:3329368,
nodule-specific protein Nlj70 [Lotus japonicus]
GI:3329366
Length = 540
Score = 27.5 bits (58), Expect = 9.3
Identities = 14/57 (24%), Positives = 27/57 (47%)
Frame = +2
Query: 527 TGPVRKEIKEPTAEAESNETKQ*LSERCESKKCYSVC*FYLHSSCVLVRLIALSYLY 697
+G + I + A ++ T + S C CYSV + C++ +++LS +Y
Sbjct: 468 SGVIASNIYDYYARKQAGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVY 524
>At1g35160.1 68414.m04360 14-3-3 protein GF14 phi (GRF4) identical
to GF14 protein phi chain GI:1493805, SP:P46077 from
[Arabidopsis thaliana]
Length = 267
Score = 27.5 bits (58), Expect = 9.3
Identities = 13/46 (28%), Positives = 24/46 (52%)
Frame = +3
Query: 93 RTAFAIRTSDWRLLRTIS*RPSSRQRCPEITTVRGSRWRSPIETSV 230
+ R + WR++ +I + SR +TT+R +RS IE+ +
Sbjct: 59 KNVIGARRASWRIISSIEQKEESRGNDDHVTTIRD--YRSKIESEL 102
>At1g27680.1 68414.m03383 glucose-1-phosphate adenylyltransferase
large subunit 2 (APL2) / ADP-glucose pyrophosphorylase
identical to SP|P55230
Length = 518
Score = 27.5 bits (58), Expect = 9.3
Identities = 15/59 (25%), Positives = 26/59 (44%)
Frame = +2
Query: 293 EEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTV 469
E+ K + +I+ G H R D ++ + + +C P ESR S G+L +
Sbjct: 198 EDAKTKNVEHVLILSGDHLYRMDYMNFVQKHIESNADITVSCLP-MDESRASDFGLLKI 255
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,386,778
Number of Sequences: 28952
Number of extensions: 288503
Number of successful extensions: 943
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 920
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 942
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1535986264
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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