Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-0855
(763 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g53480.1 68418.m06646 importin beta-2, putative similar to im... 155 4e-38
At2g16950.1 68415.m01953 importin beta-2 subunit family protein ... 50 2e-06
At2g16960.1 68415.m01954 importin beta-2 subunit family protein ... 40 0.002
At4g27640.1 68417.m03973 importin beta-2 subunit family protein ... 34 0.12
At4g16143.1 68417.m02447 importin alpha-2, putative (IMPA-2) sim... 33 0.21
At4g02150.1 68417.m00287 importin alpha-2 subunit identical to i... 33 0.21
At1g02690.2 68414.m00220 importin alpha-2 subunit, putative simi... 33 0.27
At1g02690.1 68414.m00219 importin alpha-2 subunit, putative simi... 33 0.27
At3g06720.2 68416.m00797 importin alpha-1 subunit, putative (IMP... 32 0.36
At3g06720.1 68416.m00796 importin alpha-1 subunit, putative (IMP... 32 0.36
At3g19270.1 68416.m02444 cytochrome P450 family protein similar ... 32 0.48
At2g20190.1 68415.m02361 CLIP-associating protein (CLASP) -relat... 31 0.84
At1g27210.1 68414.m03314 expressed protein 31 0.84
At1g09270.2 68414.m01036 importin alpha-1 subunit, putative (IMP... 31 0.84
At1g09270.1 68414.m01035 importin alpha-1 subunit, putative (IMP... 31 0.84
At5g62600.1 68418.m07856 transportin-SR-related contains weak si... 30 1.5
At5g65700.1 68418.m08269 leucine-rich repeat transmembrane prote... 29 3.4
At5g19890.1 68418.m02367 peroxidase, putative identical to perox... 29 3.4
At2g35050.1 68415.m04300 protein kinase family protein contains ... 29 4.5
At3g18520.2 68416.m02354 histone deacetylase family protein simi... 28 5.9
At3g18520.1 68416.m02353 histone deacetylase family protein simi... 28 5.9
At3g54910.1 68416.m06085 expressed protein various pedicted prot... 28 7.8
>At5g53480.1 68418.m06646 importin beta-2, putative similar to
importin-beta2 [Oryza sativa (japonica cultivar-group)]
GI:3983665; contains Pfam profile PF03810: Importin-beta
N-terminal domain
Length = 870
Score = 155 bits (375), Expect = 4e-38
Identities = 86/204 (42%), Positives = 120/204 (58%), Gaps = 2/204 (0%)
Frame = +1
Query: 1 VRLAATQALLNSLEFTRANFEKENERNFIMEVVCEATQSSDMRISVAALQCLVKILSLYY 180
VRLAAT+AL +L F +ANF + ER++IM VVCEAT S +++I AA +CLV I S YY
Sbjct: 192 VRLAATRALYMALGFAQANFNNDMERDYIMRVVCEATLSPEVKIRQAAFECLVSIASTYY 251
Query: 181 QHMEPYMGQALFPITLEAMKSDIDEISLQGIEFWSNVSXXXXXXXXXXXXXXXXGRPPTR 360
+ + YM Q +F IT +A++ D + ++LQ IEFWS++
Sbjct: 252 EKLAHYM-QDIFNITAKAVREDDESVALQAIEFWSSICDEEIDILEEYGGEFAGDSDV-- 308
Query: 361 TSRFYARGALQYLAPVLMQKLTKQ-DDSD-DELEWNPSKAASVCLMLLSNCCEDEIVPHV 534
++ + AL L P+L++ L KQ +D D DE WN + A CL L++ D+IVPHV
Sbjct: 309 PCFYFTKQALPGLVPLLLETLLKQEEDQDLDEGAWNIAMAGGTCLGLVARAVGDDIVPHV 368
Query: 535 LPFINCNIKSENWRYREAALMAFG 606
+PFI I +WR REAA AFG
Sbjct: 369 MPFIEEKISKPDWREREAATYAFG 392
Score = 40.3 bits (90), Expect = 0.001
Identities = 22/50 (44%), Positives = 30/50 (60%), Gaps = 1/50 (2%)
Frame = +2
Query: 605 GSILGGLGANTLKPLVENAMPTLIEAMY-DSSIAVRDTAAWTFGRIXEIV 751
GSIL G A+ L +V A+ ++ A+ D S V+DT AWT GRI E +
Sbjct: 392 GSILEGPSADKLMAIVNAALTFMLNALTNDPSNHVKDTTAWTLGRIFEFL 441
>At2g16950.1 68415.m01953 importin beta-2 subunit family protein
similar to SP|Q92973 Importin beta-2 subunit
(Transportin) {Homo sapiens}; contains Pfam profile
PF03810: Importin-beta N-terminal domain
Length = 891
Score = 50.0 bits (114), Expect = 2e-06
Identities = 25/62 (40%), Positives = 37/62 (59%), Gaps = 4/62 (6%)
Frame = +1
Query: 433 DDSDDELE-WNPSKAASVCLMLLSNCCEDEIVPHVLPFINCNIKS---ENWRYREAALMA 600
DD DD WN K ++ + +LSN DEI+P ++P I N+ + E W+ REAA++A
Sbjct: 357 DDDDDSFNVWNLRKCSAAAIDVLSNVFGDEILPALMPLIQKNLSASGDEAWKQREAAVLA 416
Query: 601 FG 606
G
Sbjct: 417 LG 418
>At2g16960.1 68415.m01954 importin beta-2 subunit family protein
contains weak hit to Pfam PF02985: HEAT repeat (4
copies); contains weak hit to Pfam PF03130: PBS lyase
HEAT-like repeat (2 copies); supported by tandem
duplication of importin beta family protein
(TIGR_Ath1:At2g16950) [Arabidopsis thaliana]; similar to
Importin beta-2 subunit (Karyopherin beta-2 subunit)
(Transportin) (M9 region interaction protein) (MIP)
(Swiss-Prot:Q92973) [Homo sapiens]
Length = 547
Score = 39.9 bits (89), Expect = 0.002
Identities = 22/65 (33%), Positives = 36/65 (55%), Gaps = 6/65 (9%)
Frame = +1
Query: 430 QDDSD---DELEWNPSKAASVCLMLLSNCCEDEIVPHVLPFINCNIK---SENWRYREAA 591
+DD + +E EWN ++ + +L+N DEI+ ++P I + E W+ REAA
Sbjct: 4 EDDDETLLNEEEWNLRACSAKFIGILANVFGDEILLTLMPLIEAKLSKFDDETWKEREAA 63
Query: 592 LMAFG 606
+ AFG
Sbjct: 64 VFAFG 68
>At4g27640.1 68417.m03973 importin beta-2 subunit family protein low
similarity to importin 4 GI:18700635 from [Homo sapiens]
Length = 1048
Score = 33.9 bits (74), Expect = 0.12
Identities = 20/73 (27%), Positives = 38/73 (52%)
Frame = +1
Query: 388 LQYLAPVLMQKLTKQDDSDDELEWNPSKAASVCLMLLSNCCEDEIVPHVLPFINCNIKSE 567
LQ + P+L + ++DD D P +A++ + L+ + VL F + + +S
Sbjct: 294 LQVMCPLLAESSDQEDDDD----LAPDRASAEVIDTLAMNLPKHVFLPVLEFASVHCQST 349
Query: 568 NWRYREAALMAFG 606
N ++REA++ A G
Sbjct: 350 NLKFREASVTALG 362
>At4g16143.1 68417.m02447 importin alpha-2, putative (IMPA-2)
similar to importin alpha 2 [Capsicum annuum]
GI:13752562; contains Pfam profiles PF01749: Importin
beta binding domain, PF00514:
Armadillo/beta-catenin-like repeat; non-consensus GG
donor splice site at exon 1 and 6; CT acceptor splice
site at exon 2
Length = 444
Score = 33.1 bits (72), Expect = 0.21
Identities = 14/48 (29%), Positives = 22/48 (45%)
Frame = +2
Query: 596 WHLGSILGGLGANTLKPLVENAMPTLIEAMYDSSIAVRDTAAWTFGRI 739
W L +I G NT + A+P ++ + S VR+ A W G +
Sbjct: 143 WALTNIASGTSENTKVVIEHGAVPIFVQLLASQSDDVREQAVWALGNV 190
>At4g02150.1 68417.m00287 importin alpha-2 subunit identical to
importin alpha-2 subunit (Karyopherin alpha-2 subunit)
(KAP alpha) SP:O04294 from [Arabidopsis thaliana]
Length = 531
Score = 33.1 bits (72), Expect = 0.21
Identities = 15/48 (31%), Positives = 23/48 (47%)
Frame = +2
Query: 596 WHLGSILGGLGANTLKPLVENAMPTLIEAMYDSSIAVRDTAAWTFGRI 739
W L +I G NT + A+P I+ + +S VR+ A W G +
Sbjct: 141 WALTNIASGTSENTNVIIESGAVPIFIQLLSSASEDVREQAVWALGNV 188
>At1g02690.2 68414.m00220 importin alpha-2 subunit, putative similar
to importin alpha-2 subunit (Karyopherin alpha-2
subunit) (KAP alpha) SP:O04294 from [Arabidopsis
thaliana]
Length = 539
Score = 32.7 bits (71), Expect = 0.27
Identities = 14/48 (29%), Positives = 23/48 (47%)
Frame = +2
Query: 596 WHLGSILGGLGANTLKPLVENAMPTLIEAMYDSSIAVRDTAAWTFGRI 739
W L +I G NT + A+P ++ + +S VR+ A W G +
Sbjct: 142 WALTNIASGTSENTRVIIDSGAVPLFVKLLSSASEEVREQAVWALGNV 189
>At1g02690.1 68414.m00219 importin alpha-2 subunit, putative similar
to importin alpha-2 subunit (Karyopherin alpha-2
subunit) (KAP alpha) SP:O04294 from [Arabidopsis
thaliana]
Length = 538
Score = 32.7 bits (71), Expect = 0.27
Identities = 14/48 (29%), Positives = 23/48 (47%)
Frame = +2
Query: 596 WHLGSILGGLGANTLKPLVENAMPTLIEAMYDSSIAVRDTAAWTFGRI 739
W L +I G NT + A+P ++ + +S VR+ A W G +
Sbjct: 142 WALTNIASGTSENTRVIIDSGAVPLFVKLLSSASEEVREQAVWALGNV 189
>At3g06720.2 68416.m00797 importin alpha-1 subunit, putative (IMPA1)
similar to importin alpha-1 subunit (Karyopherin alpha-1
subunit, KAP alpha) [Arabidopsis thaliana]
SWISS-PROT:Q96321
Length = 532
Score = 32.3 bits (70), Expect = 0.36
Identities = 14/48 (29%), Positives = 23/48 (47%)
Frame = +2
Query: 596 WHLGSILGGLGANTLKPLVENAMPTLIEAMYDSSIAVRDTAAWTFGRI 739
W L +I G +T + NA+P ++ + S VR+ A W G +
Sbjct: 138 WALTNIASGTSDHTKVVIDHNAVPIFVQLLASPSDDVREQAVWALGNV 185
>At3g06720.1 68416.m00796 importin alpha-1 subunit, putative (IMPA1)
similar to importin alpha-1 subunit (Karyopherin alpha-1
subunit, KAP alpha) [Arabidopsis thaliana]
SWISS-PROT:Q96321
Length = 532
Score = 32.3 bits (70), Expect = 0.36
Identities = 14/48 (29%), Positives = 23/48 (47%)
Frame = +2
Query: 596 WHLGSILGGLGANTLKPLVENAMPTLIEAMYDSSIAVRDTAAWTFGRI 739
W L +I G +T + NA+P ++ + S VR+ A W G +
Sbjct: 138 WALTNIASGTSDHTKVVIDHNAVPIFVQLLASPSDDVREQAVWALGNV 185
>At3g19270.1 68416.m02444 cytochrome P450 family protein similar to
Cytochrome P450 85 (SP:Q43147) {Lycopersicon
esculentum};
Length = 468
Score = 31.9 bits (69), Expect = 0.48
Identities = 10/39 (25%), Positives = 23/39 (58%)
Frame = +1
Query: 175 YYQHMEPYMGQALFPITLEAMKSDIDEISLQGIEFWSNV 291
Y+ H+ + + +P T+ + DI+ I+L ++ W+N+
Sbjct: 123 YHSHIRKLVQSSFYPETIRKLIPDIEHIALSSLQSWANM 161
>At2g20190.1 68415.m02361 CLIP-associating protein (CLASP) -related
similar to CLIP-associating protein CLASP2 (GI:13508651)
[Rattus norvegicus]
Length = 1439
Score = 31.1 bits (67), Expect = 0.84
Identities = 21/78 (26%), Positives = 39/78 (50%)
Frame = +1
Query: 4 RLAATQALLNSLEFTRANFEKENERNFIMEVVCEATQSSDMRISVAALQCLVKILSLYYQ 183
R+AA + L LE +R + E +++ + + S+ R+S ALQ L L +
Sbjct: 16 RMAAVERLHQLLEASRKSLSPA-EVTSLVDSCLDLLKDSNFRVSQGALQALASAAVLAGE 74
Query: 184 HMEPYMGQALFPITLEAM 237
H++ ++ AL P +E +
Sbjct: 75 HLKLHL-NALVPAVVERL 91
>At1g27210.1 68414.m03314 expressed protein
Length = 625
Score = 31.1 bits (67), Expect = 0.84
Identities = 17/46 (36%), Positives = 27/46 (58%)
Frame = +2
Query: 602 LGSILGGLGANTLKPLVENAMPTLIEAMYDSSIAVRDTAAWTFGRI 739
+GSI+ GA T KP+++ +P LIE + A R +AA G++
Sbjct: 218 VGSIITAGGAGT-KPVLDWLVPVLIEFLSSEDWAARKSAAEALGKV 262
>At1g09270.2 68414.m01036 importin alpha-1 subunit, putative (IMPA4)
similar to importin alpha-1 subunit (Karyopherin alpha-1
subunit, KAP alpha) [Arabidopsis thaliana]
SWISS-PROT:Q96321
Length = 538
Score = 31.1 bits (67), Expect = 0.84
Identities = 12/48 (25%), Positives = 24/48 (50%)
Frame = +2
Query: 596 WHLGSILGGLGANTLKPLVENAMPTLIEAMYDSSIAVRDTAAWTFGRI 739
W L ++ G +T + + A+P ++ + +S VR+ A W G +
Sbjct: 145 WALTNVASGTSDHTRVVIEQGAVPIFVKLLTSASDDVREQAVWALGNV 192
>At1g09270.1 68414.m01035 importin alpha-1 subunit, putative (IMPA4)
similar to importin alpha-1 subunit (Karyopherin alpha-1
subunit, KAP alpha) [Arabidopsis thaliana]
SWISS-PROT:Q96321
Length = 538
Score = 31.1 bits (67), Expect = 0.84
Identities = 12/48 (25%), Positives = 24/48 (50%)
Frame = +2
Query: 596 WHLGSILGGLGANTLKPLVENAMPTLIEAMYDSSIAVRDTAAWTFGRI 739
W L ++ G +T + + A+P ++ + +S VR+ A W G +
Sbjct: 145 WALTNVASGTSDHTRVVIEQGAVPIFVKLLTSASDDVREQAVWALGNV 192
>At5g62600.1 68418.m07856 transportin-SR-related contains weak
similarity to transportin-SR (GI:5052414) [Homo sapiens]
Length = 958
Score = 30.3 bits (65), Expect = 1.5
Identities = 14/58 (24%), Positives = 29/58 (50%), Gaps = 3/58 (5%)
Frame = +1
Query: 454 EWNPSKAASVCLMLLSN---CCEDEIVPHVLPFINCNIKSENWRYREAALMAFGFNSW 618
+W P++A C+ +SN E E++P V+ + N+ + + A L+ ++ W
Sbjct: 449 DWRPAEAILFCIWAISNYVSVVEAEVMPQVMALLQ-NLPQQAQLLQTACLLVGAYSKW 505
>At5g65700.1 68418.m08269 leucine-rich repeat transmembrane protein
kinase, putative
Length = 1003
Score = 29.1 bits (62), Expect = 3.4
Identities = 13/46 (28%), Positives = 21/46 (45%)
Frame = -1
Query: 484 KQKQPLTDSIPIHHQNHLVLLVFASRQVQGIEVPLLHRIVKF*LEV 347
K P SIPIH H+ + + Q +E P + +V+ E+
Sbjct: 923 KVLDPRLSSIPIHEVTHVFYVAMLCVEEQAVERPTMREVVQILTEI 968
>At5g19890.1 68418.m02367 peroxidase, putative identical to
peroxidase [Arabidopsis thaliana]
gi|1403134|emb|CAA67092
Length = 328
Score = 29.1 bits (62), Expect = 3.4
Identities = 17/53 (32%), Positives = 28/53 (52%)
Frame = +2
Query: 575 DTEKLL*WHLGSILGGLGANTLKPLVENAMPTLIEAMYDSSIAVRDTAAWTFG 733
D+EKL ++ S G +T+K VENA P ++ ++A RD+ + G
Sbjct: 87 DSEKLAIPNINSARGFEVIDTIKAAVENACPGVVSCADILTLAARDSVVLSGG 139
>At2g35050.1 68415.m04300 protein kinase family protein contains Pfam
profile: PF00069 Eukaryotic protein kinase domain
Length = 1257
Score = 28.7 bits (61), Expect = 4.5
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = +2
Query: 599 HLGSILGGLGANTLKPLVEN 658
H G+I+GG+ NTL+P V N
Sbjct: 1186 HYGAIIGGIVNNTLRPTVPN 1205
>At3g18520.2 68416.m02354 histone deacetylase family protein similar
to SP|P53973 Histone deacetylase HDA1 {Saccharomyces
cerevisiae}; contains Pfam profile PF00850: Histone
deacetylase family; AT-acceptor splice site at intron 7
Length = 556
Score = 28.3 bits (60), Expect = 5.9
Identities = 15/63 (23%), Positives = 31/63 (49%)
Frame = +1
Query: 421 LTKQDDSDDELEWNPSKAASVCLMLLSNCCEDEIVPHVLPFINCNIKSENWRYREAALMA 600
L DD DD+ +W P +A + + CC + + + ++C I E ++E+ ++
Sbjct: 66 LNDDDDEDDDCDWEPVQAP---MEFVKWCCVNCTMSNPGDMVHCCICGE---HKESGILR 119
Query: 601 FGF 609
G+
Sbjct: 120 HGY 122
>At3g18520.1 68416.m02353 histone deacetylase family protein similar
to SP|P53973 Histone deacetylase HDA1 {Saccharomyces
cerevisiae}; contains Pfam profile PF00850: Histone
deacetylase family; AT-acceptor splice site at intron 7
Length = 552
Score = 28.3 bits (60), Expect = 5.9
Identities = 15/63 (23%), Positives = 31/63 (49%)
Frame = +1
Query: 421 LTKQDDSDDELEWNPSKAASVCLMLLSNCCEDEIVPHVLPFINCNIKSENWRYREAALMA 600
L DD DD+ +W P +A + + CC + + + ++C I E ++E+ ++
Sbjct: 66 LNDDDDEDDDCDWEPVQAP---MEFVKWCCVNCTMSNPGDMVHCCICGE---HKESGILR 119
Query: 601 FGF 609
G+
Sbjct: 120 HGY 122
>At3g54910.1 68416.m06085 expressed protein various pedicted
proteins, Arabidopsis thaliana
Length = 373
Score = 27.9 bits (59), Expect = 7.8
Identities = 15/40 (37%), Positives = 19/40 (47%)
Frame = -3
Query: 668 LAWHFPLMVSEC*LQDHQELNPNAIRAASLYRQFSLLMLQ 549
L WH P V EC L Q LN +A R R ++ L+
Sbjct: 293 LYWHQPSTVPECMLLSLQTLNLSAYRGTPEERDLAVYFLK 332
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,349,922
Number of Sequences: 28952
Number of extensions: 270492
Number of successful extensions: 749
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 725
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 743
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1702303248
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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