Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-0850
(584 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 308 5e-85
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 308 5e-85
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 119 3e-28
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 116 3e-27
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 60 3e-10
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 45 7e-06
SPAC22H12.02 |tfg3|taf14|transcription factor TFIIF complex subu... 29 0.50
SPAC21E11.06 |tif224||translation initiation factor eIF2B delta ... 28 1.2
SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces po... 27 2.7
SPBC1604.05 |pgi1||glucose-6-phosphate isomerase |Schizosaccharo... 26 3.5
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 26 4.7
SPCC622.12c |||NADP-specific glutamate dehydrogenase |Schizosacc... 26 4.7
SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccha... 25 6.2
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 25 8.1
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 25 8.1
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 308 bits (755), Expect = 5e-85
Identities = 146/193 (75%), Positives = 164/193 (84%)
Frame = +1
Query: 4 AQKYRMEMLYEGPHDDEAAIGIKSCDPEAPLMMYVSKMVPTSDKGRFYAFGRVFSGKVVT 183
AQ+YR E LYEGP DDE A+GI++CD APLM+YVSKMVPTSD+GRFYAFGRVFSG V +
Sbjct: 348 AQQYRAETLYEGPMDDECAVGIRNCDANAPLMIYVSKMVPTSDRGRFYAFGRVFSGTVRS 407
Query: 184 GQKARIMGPNFTPGKKEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTG 363
G K RI GPN+ PGKK+DL+ K IQRT+LMMG +E IED P+GNI GLVGVDQFLVK+G
Sbjct: 408 GLKVRIQGPNYVPGKKDDLFIKAIQRTVLMMGSRIEPIEDCPAGNIIGLVGVDQFLVKSG 467
Query: 364 TITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGE 543
T+TT + AHNMKVMKFSVSPVV+VAVE KN DLPKLVEGLKRL+KSDP V C ESGE
Sbjct: 468 TLTTSEVAHNMKVMKFSVSPVVQVAVEVKNGNDLPKLVEGLKRLSKSDPCVLCTTSESGE 527
Query: 544 HIVAGAGELHLEI 582
HIVAGAGELHLEI
Sbjct: 528 HIVAGAGELHLEI 540
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 308 bits (755), Expect = 5e-85
Identities = 146/193 (75%), Positives = 164/193 (84%)
Frame = +1
Query: 4 AQKYRMEMLYEGPHDDEAAIGIKSCDPEAPLMMYVSKMVPTSDKGRFYAFGRVFSGKVVT 183
AQ+YR E LYEGP DDE A+GI++CD APLM+YVSKMVPTSD+GRFYAFGRVFSG V +
Sbjct: 348 AQQYRAETLYEGPMDDECAVGIRNCDANAPLMIYVSKMVPTSDRGRFYAFGRVFSGTVRS 407
Query: 184 GQKARIMGPNFTPGKKEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTG 363
G K RI GPN+ PGKK+DL+ K IQRT+LMMG +E IED P+GNI GLVGVDQFLVK+G
Sbjct: 408 GLKVRIQGPNYVPGKKDDLFIKAIQRTVLMMGSRIEPIEDCPAGNIIGLVGVDQFLVKSG 467
Query: 364 TITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGE 543
T+TT + AHNMKVMKFSVSPVV+VAVE KN DLPKLVEGLKRL+KSDP V C ESGE
Sbjct: 468 TLTTSEVAHNMKVMKFSVSPVVQVAVEVKNGNDLPKLVEGLKRLSKSDPCVLCTTSESGE 527
Query: 544 HIVAGAGELHLEI 582
HIVAGAGELHLEI
Sbjct: 528 HIVAGAGELHLEI 540
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 119 bits (287), Expect = 3e-28
Identities = 67/188 (35%), Positives = 106/188 (56%), Gaps = 5/188 (2%)
Frame = +1
Query: 31 YEGPHDDEAAIGI--KSCDPEAPLMMYVSKMVPTSDKGRFYAFGRVFSGKVVTGQKARIM 204
Y GP + I S + APL+M+V+K+ T D FYAF RV+SG+V GQK +++
Sbjct: 464 YIGPINSSIGKAILEMSREESAPLVMHVTKLYNTVDANNFYAFARVYSGQVKKGQKVKVL 523
Query: 205 GPNFTPGKKEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITTFK- 381
G N++ +ED+ I + RY ++ +G + L GVD + KT TI +
Sbjct: 524 GENYSLEDEEDMVVAHIAEICVPCARYRLHVDGAVAGMLVLLGGVDNSISKTATIVSDNL 583
Query: 382 --NAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVA 555
+ + + + V +VAVEP NP++LPKL++GL++ KS P+ EESGEH +
Sbjct: 584 KDDPYIFRPIAHMSESVFKVAVEPHNPSELPKLLDGLRKTNKSYPLSITKVEESGEHTIF 643
Query: 556 GAGELHLE 579
G GE++++
Sbjct: 644 GTGEMYMD 651
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 116 bits (279), Expect = 3e-27
Identities = 57/151 (37%), Positives = 90/151 (59%), Gaps = 1/151 (0%)
Frame = +1
Query: 130 DKGRFYAFGRVFSGKVVTGQKARIMGPNFTPGKKEDLYEK-TIQRTILMMGRYVEAIEDV 306
DK F R++SG + GQ+ + GP + P E K T++ LMMG+ + +E V
Sbjct: 464 DKDILIGFARIYSGTISVGQEVYVYGPKYDPVNPEKHITKVTVESLYLMMGQELVYLETV 523
Query: 307 PSGNICGLVGVDQFLVKTGTITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGL 486
P+GN+ + G+ +++T T+ + N N+ + + P+VRVA+EP P ++ KLV GL
Sbjct: 524 PAGNVFAIGGLAGTVLRTATLCSSPNGPNLVGVTQQMEPIVRVALEPVRPFEMNKLVTGL 583
Query: 487 KRLAKSDPMVQCINEESGEHIVAGAGELHLE 579
L ++DP VQ EE+GEH++ AGE+HLE
Sbjct: 584 DMLNQADPCVQIAVEENGEHVIMCAGEIHLE 614
Score = 29.5 bits (63), Expect = 0.38
Identities = 12/32 (37%), Positives = 22/32 (68%), Gaps = 2/32 (6%)
Frame = +1
Query: 46 DDEAAIGIKSCDP--EAPLMMYVSKMVPTSDK 135
D + + ++SCD E P+++Y+SKMV S++
Sbjct: 376 DPDITLAMESCDASKEQPVLVYISKMVAFSER 407
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 59.7 bits (138), Expect = 3e-10
Identities = 52/183 (28%), Positives = 84/183 (45%), Gaps = 9/183 (4%)
Frame = +1
Query: 61 IGIKSCDPEAPLMMYVSKMVPTS------DKGRF--YAFGRVFSGKVVTGQKARIMGPNF 216
I + + D E P+ + S P ++GRF + R++ G + G I N
Sbjct: 358 IALNAADSEKPVSLVPSSEKPLVALAFKLEEGRFGQLTYLRIYQGTLKRGNY--IYNVNS 415
Query: 217 TPGKKEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITTFKNAHNM 396
T K + R + M +E IE V +G IC L G++ T T ++ M
Sbjct: 416 TKKIK-------VSRLVRMHSNDMEEIEKVEAGGICALFGIE--CASGDTFTDGSVSYTM 466
Query: 397 KVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQC-INEESGEHIVAGAGELH 573
M F PV+ ++++PK+ D + L R + DP + ++ ES E I++G GELH
Sbjct: 467 TSM-FVPEPVISLSLKPKSK-DTTSFSKALNRFQREDPTFRVQLDNESKETIISGMGELH 524
Query: 574 LEI 582
LE+
Sbjct: 525 LEV 527
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 45.2 bits (102), Expect = 7e-06
Identities = 45/173 (26%), Positives = 78/173 (45%), Gaps = 9/173 (5%)
Frame = +1
Query: 91 PLMMYVSKMVPTSDKGRFYAFGRVFSGKVVTGQKARIMGPNFTPGKKEDLYEKTIQRTIL 270
PL+ + K++ + +G + RV G + G F P K+ E+ I R
Sbjct: 337 PLLAKIFKVIHHASRG-ILTYVRVNEGTLSRGMMM------FNPRTKKS--ERAI-RLYN 386
Query: 271 MMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTIT----TFKNAHNMKVMKFSV----SPV 426
+ + ++ + +GNI + G+ QF TG I KN H SV PV
Sbjct: 387 VFADQTQEVDCISAGNIGVISGIKQF--HTGDIIINKENSKNFHEYLSGNQSVISIPEPV 444
Query: 427 VRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINE-ESGEHIVAGAGELHLEI 582
++EP + D P L+E L + + DP + + E+G+ ++ G G +HL++
Sbjct: 445 CIASIEPYSLKDEPALLEALANMNREDPSFRYTQDLENGQLLIQGMGIMHLQV 497
>SPAC22H12.02 |tfg3|taf14|transcription factor TFIIF complex subunit
Tfg3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 241
Score = 29.1 bits (62), Expect = 0.50
Identities = 16/49 (32%), Positives = 26/49 (53%), Gaps = 6/49 (12%)
Frame = +1
Query: 454 PADLPKLVEGLKRLAKSD--PMVQCINEESGEHIVA----GAGELHLEI 582
P D+ KL EGL++L + D +VQ +NE + GE H+++
Sbjct: 172 PVDMDKLAEGLQKLQEDDLLQVVQMVNENKTPDMYVRNDIEGGEFHIDL 220
>SPAC21E11.06 |tif224||translation initiation factor eIF2B delta
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 467
Score = 27.9 bits (59), Expect = 1.2
Identities = 12/33 (36%), Positives = 21/33 (63%), Gaps = 2/33 (6%)
Frame = +1
Query: 331 VGVDQFLVKTGTITTFKNAHNMKV--MKFSVSP 423
+GVD F K G + +K+ N+K+ +K+ V+P
Sbjct: 404 MGVDDFEEKPGVLANWKSVKNLKLLSLKYDVTP 436
>SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 793
Score = 26.6 bits (56), Expect = 2.7
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = +1
Query: 340 DQFLVKTGTITTFKNAHNMKVMKFSVSPVV 429
D FLVK G + ++ +N+ FSVS +V
Sbjct: 82 DTFLVKYGDLINIQDPYNLNKTLFSVSDLV 111
>SPBC1604.05 |pgi1||glucose-6-phosphate isomerase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 550
Score = 26.2 bits (55), Expect = 3.5
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = +1
Query: 34 EGPHDDEAAIGIKSCDPEAPLMMYVSKMVPTSD 132
+G H EA +K CDPE L + SK T++
Sbjct: 193 DGTHLAEA---LKKCDPETTLFLIASKTFTTAE 222
>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 4717
Score = 25.8 bits (54), Expect = 4.7
Identities = 19/68 (27%), Positives = 32/68 (47%)
Frame = +1
Query: 100 MYVSKMVPTSDKGRFYAFGRVFSGKVVTGQKARIMGPNFTPGKKEDLYEKTIQRTILMMG 279
+YV VP D RFY + ++ + T + + +F P +D EK + L G
Sbjct: 2356 IYVWTNVPDCDISRFYCYTKMLGEWMFTLTEKTKLLESFLP---KDSLEKFSELQNLSTG 2412
Query: 280 RYVEAIED 303
+++AI D
Sbjct: 2413 LHMQAIWD 2420
>SPCC622.12c |||NADP-specific glutamate dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 451
Score = 25.8 bits (54), Expect = 4.7
Identities = 15/45 (33%), Positives = 25/45 (55%), Gaps = 1/45 (2%)
Frame = +1
Query: 205 GPNFTP-GKKEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVG 336
G +F P GK ++ + Q + + RY+ DVP+G+I G+ G
Sbjct: 115 GSDFDPKGKSDNEIRRFSQAFMRQLFRYIGPQTDVPAGDI-GVTG 158
>SPAC23E2.03c |ste7||meiotic suppressor protein
Ste7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 569
Score = 25.4 bits (53), Expect = 6.2
Identities = 10/33 (30%), Positives = 22/33 (66%), Gaps = 2/33 (6%)
Frame = -1
Query: 569 SSPAPATMCSPDSSL--IHCTMGSDLARRLRPS 477
S+ +P+T+CSPD+++ ++ G L ++ P+
Sbjct: 403 SNVSPSTICSPDNNVTFVNLAPGEKLTFKIEPN 435
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 25.0 bits (52), Expect = 8.1
Identities = 27/98 (27%), Positives = 44/98 (44%), Gaps = 4/98 (4%)
Frame = -1
Query: 569 SSPAPATMCSPDSSLIHCTMGSD-LARRLRPS---TSLGRSAGFLGSTATRTTGDTLNFI 402
SSP P T S +S T GS L+ + P+ TS ++ + T+T +T + +
Sbjct: 219 SSPLPTTSTSCTTSTSIPTGGSSSLSTPITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPL 278
Query: 401 TFMLWAFLKVVMVPVLTKN*STPTRPQMLPEGTSSIAS 288
+ +P T N +TP P + P TSS ++
Sbjct: 279 PTTSTSCTTSTSIPP-TGNSTTPVTPTVPPTSTSSTST 315
>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 897
Score = 25.0 bits (52), Expect = 8.1
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = +2
Query: 122 RPPTRVVSTPLDAFSL 169
RPPTR+ STP ++ SL
Sbjct: 104 RPPTRLTSTPSNSSSL 119
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,645,696
Number of Sequences: 5004
Number of extensions: 56235
Number of successful extensions: 180
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 176
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 252150250
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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