Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-0841
(629 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NA... 222 2e-58
At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NA... 220 7e-58
At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NA... 219 2e-57
At5g14460.1 68418.m01692 pseudouridylate synthase TruB family pr... 33 0.21
At2g46980.2 68415.m05869 expressed protein 29 1.9
At2g46980.1 68415.m05868 expressed protein 29 1.9
At1g15870.1 68414.m01904 mitochondrial glycoprotein family prote... 29 1.9
At5g56100.1 68418.m06999 glycine-rich protein / oleosin contains... 29 3.4
At4g40050.1 68417.m05671 expressed protein 28 4.4
At5g63070.1 68418.m07914 40S ribosomal protein S15, putative 28 5.9
At5g08390.1 68418.m00988 transducin family protein / WD-40 repea... 28 5.9
At4g08470.1 68417.m01398 mitogen-activated protein kinase, putat... 28 5.9
At3g02250.1 68416.m00206 expressed protein contains Pfam PF03138... 28 5.9
At5g06940.1 68418.m00784 leucine-rich repeat family protein cont... 27 7.8
>At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
NADP-isocitrate dehydrogenase GI:5764653 from [Citrus
limon]; Nicotiana tabacum SP|P50218
Length = 416
Score = 222 bits (542), Expect = 2e-58
Identities = 102/144 (70%), Positives = 118/144 (81%), Gaps = 1/144 (0%)
Frame = +3
Query: 198 KIKA-GPVVDILGDEMTRIIWDLIKEKLILPFLDIELHVYDLGMENRDKTDDQVTIDCAE 374
KIK PVV++ GDEMTR+IW IK+KLI PFL++++ +DLG+ NRD TDD+VTI+ AE
Sbjct: 5 KIKVINPVVEMDGDEMTRVIWKFIKDKLIFPFLELDIKYFDLGLPNRDFTDDKVTIETAE 64
Query: 375 AIKKYNVGIKCATITPDEKRVEEFKLKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVT 554
A KYNV IKCATITPDE RV EF LKKMW+SPNGTIRNIL GTVFRE IIC+NIPRLV
Sbjct: 65 ATLKYNVAIKCATITPDEARVREFGLKKMWRSPNGTIRNILNGTVFREPIICRNIPRLVP 124
Query: 555 GWDKPIIIGRHAHADQYKATDFVV 626
GW KPI IGRHA DQY+ATD +V
Sbjct: 125 GWTKPICIGRHAFGDQYRATDLIV 148
>At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase (NADP+) [Nicotiana tabacum]
GI:3021512; contains Pfam domain PF00180: dehydrogenase,
isocitrate/isopropylmalate family
Length = 485
Score = 220 bits (537), Expect = 7e-58
Identities = 97/138 (70%), Positives = 117/138 (84%)
Frame = +3
Query: 213 PVVDILGDEMTRIIWDLIKEKLILPFLDIELHVYDLGMENRDKTDDQVTIDCAEAIKKYN 392
P+V++ GDEMTR+IW +IKEKLILP+LD+++ +DLG+ NRD TDD+VT++ AEA KYN
Sbjct: 81 PIVEMDGDEMTRVIWSMIKEKLILPYLDLDIKYFDLGILNRDATDDKVTVESAEAALKYN 140
Query: 393 VGIKCATITPDEKRVEEFKLKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWDKPI 572
V IKCATITPDE RV+EF LK MW+SPNGTIRNIL GTVFRE I+C NIPRLV GW+KPI
Sbjct: 141 VAIKCATITPDEGRVKEFGLKSMWRSPNGTIRNILDGTVFREPIMCSNIPRLVPGWEKPI 200
Query: 573 IIGRHAHADQYKATDFVV 626
IGRHA DQY+ATD V+
Sbjct: 201 CIGRHAFGDQYRATDTVI 218
>At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase SP|Q40345 from [Medicago
sativa]
Length = 410
Score = 219 bits (534), Expect = 2e-57
Identities = 99/144 (68%), Positives = 120/144 (83%), Gaps = 1/144 (0%)
Frame = +3
Query: 198 KIK-AGPVVDILGDEMTRIIWDLIKEKLILPFLDIELHVYDLGMENRDKTDDQVTIDCAE 374
KIK A P+V++ GDEMTR+IW IK+KLI PF+++++ +DLG+ +RD TDD+VTI+ AE
Sbjct: 5 KIKVANPIVEMDGDEMTRVIWKSIKDKLITPFVELDIKYFDLGLPHRDATDDKVTIESAE 64
Query: 375 AIKKYNVGIKCATITPDEKRVEEFKLKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVT 554
A KKYNV IKCATITPDE RV EF LK+MW+SPNGTIRNIL GTVFRE IICKN+P+LV
Sbjct: 65 ATKKYNVAIKCATITPDEGRVTEFGLKQMWRSPNGTIRNILNGTVFREPIICKNVPKLVP 124
Query: 555 GWDKPIIIGRHAHADQYKATDFVV 626
GW KPI IGRHA DQY+ATD V+
Sbjct: 125 GWTKPICIGRHAFGDQYRATDAVI 148
>At5g14460.1 68418.m01692 pseudouridylate synthase TruB family
protein similar to SP|P09171 tRNA pseudouridine synthase
B (EC 4.2.1.70) (tRNA pseudouridine 55 synthase)
(Pseudouridylate synthase) (Uracil hydrolyase)
{Escherichia coli O157:H7}; contains Pfam profile
PF01509: TruB family pseudouridylate synthase (N
terminal domain)
Length = 540
Score = 32.7 bits (71), Expect = 0.21
Identities = 27/101 (26%), Positives = 43/101 (42%)
Frame = +3
Query: 267 KEKLILPFLDIELHVYDLGMENRDKTDDQVTIDCAEAIKKYNVGIKCATITPDEKRVEEF 446
K+K++ V +GMEN D+ DD++ K+ + + EK+VE+F
Sbjct: 222 KKKVVAALGGKRSEVKRIGMENCDEDDDKLVFFDEVKEKEEKKKSEDDVVVVTEKKVEQF 281
Query: 447 KLKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWDKP 569
K + KSPN + + G E + L WD P
Sbjct: 282 -FKGLTKSPNE--KGMASGGGDGEPFLVTRNGELPPRWDGP 319
>At2g46980.2 68415.m05869 expressed protein
Length = 516
Score = 29.5 bits (63), Expect = 1.9
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = -1
Query: 599 ISMSMTSNDDGFVPPCYKSRYILANNSLPENSTTKNIT 486
+S SNDD + P + S I ++ PEN+T + +T
Sbjct: 229 LSQDKGSNDDPLIKPRHNSDSIETDSESPENATRRPVT 266
>At2g46980.1 68415.m05868 expressed protein
Length = 516
Score = 29.5 bits (63), Expect = 1.9
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = -1
Query: 599 ISMSMTSNDDGFVPPCYKSRYILANNSLPENSTTKNIT 486
+S SNDD + P + S I ++ PEN+T + +T
Sbjct: 229 LSQDKGSNDDPLIKPRHNSDSIETDSESPENATRRPVT 266
>At1g15870.1 68414.m01904 mitochondrial glycoprotein family protein
/ MAM33 family protein similar to SUAPRGA1 [Emericella
nidulans] GI:6562379; contains Pfam profile PF02330:
Mitochondrial glycoprotein
Length = 242
Score = 29.5 bits (63), Expect = 1.9
Identities = 22/83 (26%), Positives = 36/83 (43%)
Frame = +3
Query: 141 PTNLKSTCLFIEVERYNMSKIKAGPVVDILGDEMTRIIWDLIKEKLILPFLDIELHVYDL 320
P ++ T F+ N GP + DE+ ++ ++E+ I L LH Y
Sbjct: 162 PDTIQITKFFVRKSSQNSPNAYIGPEFQEMEDELQDSVYRFLEERGISDDLAEFLHQY-- 219
Query: 321 GMENRDKTDDQVTIDCAEAIKKY 389
M+N+DK + I E +K Y
Sbjct: 220 -MKNKDKAE---YIRWMETVKSY 238
>At5g56100.1 68418.m06999 glycine-rich protein / oleosin contains
Pfam PF01277: Oleosin domain
Length = 150
Score = 28.7 bits (61), Expect = 3.4
Identities = 12/39 (30%), Positives = 24/39 (61%)
Frame = -2
Query: 286 GRISFSLIRSQIILVISSPRMSTTGPAFILDILYLSTSM 170
G +SFS + + + +I+SP + PAF++ + L ++M
Sbjct: 41 GLMSFSFVATVTLFLIASPLLLIFAPAFMVTVAVLVSAM 79
>At4g40050.1 68417.m05671 expressed protein
Length = 599
Score = 28.3 bits (60), Expect = 4.4
Identities = 17/53 (32%), Positives = 31/53 (58%), Gaps = 3/53 (5%)
Frame = -1
Query: 605 VLISMSMTSNDDGFVPPCYKS-RYILANNSLPENSTTKNITD--GPIRAFPHF 456
VL S S T +DD ++P C+ + +L++ S P S+ + + + G ++F HF
Sbjct: 545 VLTSFSRTEDDDPYLPQCHPNLPELLSSVSKPVQSSVQRLAEHLGVAKSF-HF 596
>At5g63070.1 68418.m07914 40S ribosomal protein S15, putative
Length = 160
Score = 27.9 bits (59), Expect = 5.9
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = +2
Query: 518 GYYLQEYTETCNRVGQTHHHWTSCSC 595
G+YL E++ TC +V HH C C
Sbjct: 124 GHYLAEFSMTCKKV--NHHRPRICGC 147
>At5g08390.1 68418.m00988 transducin family protein / WD-40 repeat
family protein similar to katanin p80 subunit
[Strongylocentrotus purpuratus] GI:3005601; contains
Pfam profile PF00400: WD domain, G-beta repeat
Length = 871
Score = 27.9 bits (59), Expect = 5.9
Identities = 23/81 (28%), Positives = 33/81 (40%), Gaps = 12/81 (14%)
Frame = +3
Query: 231 GDEMTRIIWDLIKEKLILPF---------LDIELHVYDLGMENRDKTDDQVTIDCAEAIK 383
G++ +WDL KL+ F LD H + L + DKT ++ E I
Sbjct: 255 GEDNVVKVWDLTAGKLLHEFKSHEGKIQSLDFHPHEFLLATGSADKTVKFWDLETFELIG 314
Query: 384 K---YNVGIKCATITPDEKRV 437
G++C T PD K V
Sbjct: 315 SGGTETTGVRCLTFNPDGKSV 335
>At4g08470.1 68417.m01398 mitogen-activated protein kinase, putative
similar to mitogen-activated protein kinase [Arabidopsis
thaliana] gi|1255448|dbj|BAA09057; contains Pfam
PF00069: Protein kinase domain
Length = 560
Score = 27.9 bits (59), Expect = 5.9
Identities = 21/84 (25%), Positives = 37/84 (44%), Gaps = 4/84 (4%)
Frame = +3
Query: 372 EAIKKYNVGIK--CATITPDEKRVEEFKLKKMWKSPNGTIRNILGGTVFREAIICKNI-- 539
EAI +VG + C+ +TP + + +K S G + + +N
Sbjct: 237 EAISDSSVGSESTCSLMTPSMEFPDRISFRKRDFSEEGPSGRVKEKRKLMRNKLIENFRK 296
Query: 540 PRLVTGWDKPIIIGRHAHADQYKA 611
P +T W K ++GR ++A Y+A
Sbjct: 297 PEDITSWLKGQLLGRGSYASVYEA 320
>At3g02250.1 68416.m00206 expressed protein contains Pfam PF03138:
Plant protein family. The function of this family of
plant proteins is unknown; previously annotated as
'auxin-independent growth promoter -related' based on
similarity to axi 1 protein (GB:X80301) (GI:559920) from
[Nicotiana tabacum], which, due to scienitific fraud was
retracted. Retraction in: Schell J. EMBO J 1999 May
17;18(10):2908. PMID:10400497.
Length = 512
Score = 27.9 bits (59), Expect = 5.9
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = -2
Query: 514 LKTVPPRILRMVPLGLFH 461
LK +PPR+ R V LGL+H
Sbjct: 176 LKELPPRLKRRVRLGLYH 193
>At5g06940.1 68418.m00784 leucine-rich repeat family protein
contains protein kinase domain, Pfam:PF00069; contains
leucine-rich repeats, Pfam:PF00560
Length = 872
Score = 27.5 bits (58), Expect = 7.8
Identities = 15/46 (32%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
Frame = -1
Query: 614 SCFVLISMSMTSND-DGFVPPCYKSRYILANNSLPENSTTKNITDG 480
+C L+S+S+ N G +PP ++L L +NS T I G
Sbjct: 434 NCKKLVSLSLAGNAFTGEIPPSLADLHVLTYLDLSDNSLTGLIPQG 479
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,800,998
Number of Sequences: 28952
Number of extensions: 284960
Number of successful extensions: 697
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 684
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 697
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1285411824
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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