Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-0735
(695 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g11900.1 68416.m01459 amino acid transporter family protein l... 43 2e-04
At4g38250.1 68417.m05402 amino acid transporter family protein l... 43 2e-04
At2g42005.1 68415.m05196 amino acid transporter family protein l... 42 5e-04
At1g80510.1 68414.m09435 amino acid transporter family protein s... 40 0.002
At5g38820.1 68418.m04695 amino acid transporter family protein l... 34 0.10
At3g30390.1 68416.m03836 amino acid transporter family protein l... 32 0.32
At2g40420.1 68415.m04985 amino acid transporter family protein s... 31 0.55
At5g41800.1 68418.m05089 amino acid transporter family protein s... 31 0.96
At1g44100.1 68414.m05094 amino acid permease 5, putative (AAP5) ... 31 0.96
At5g09220.1 68418.m01045 amino acid permease 2 (AAP2) identical ... 30 1.7
At2g39130.1 68415.m04807 amino acid transporter family protein b... 30 1.7
At1g77380.1 68414.m09011 amino acid carrier, putative / amino ac... 30 1.7
At5g24170.1 68418.m02844 expressed protein 29 2.9
At1g10010.1 68414.m01129 amino acid permease, putative similar t... 29 2.9
At3g56200.1 68416.m06246 amino acid transporter family protein l... 29 3.9
At5g63850.1 68418.m08015 amino acid transporter 4, putative (AAP... 28 6.8
At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot... 28 6.8
At1g58360.1 68414.m06638 amino acid permease I (AAP1) identical ... 28 6.8
At4g08690.1 68417.m01432 SEC14 cytosolic factor family protein /... 27 9.0
>At3g11900.1 68416.m01459 amino acid transporter family protein low
similarity to proton/amino acid transporter 1 [Mus
musculus] GI:21908024; belongs to INTERPRO:IPR002422
amino acid/polyamine transporter, family II
Length = 432
Score = 43.2 bits (97), Expect = 2e-04
Identities = 18/59 (30%), Positives = 37/59 (62%)
Frame = +1
Query: 358 TSSFGALAHLLKASLGSGVLAMPLAFKNAGLLVGCIGTMLIGLICGHVIHILVKTSQQL 534
TS+ L +++ + +G+GVL +P AF+ AG L G +G +++G + + +L++ +L
Sbjct: 31 TSALQTLGNIIVSIVGTGVLGLPYAFRIAGWLAGSLGVIIVGFATYYCMLLLIQCRDKL 89
>At4g38250.1 68417.m05402 amino acid transporter family protein low
similarity to lysosomal amino acid transporter 1 [Rattus
norvegicus] GI:14571904; contains Pfam profile PF01490:
Transmembrane amino acid transporter protein
Length = 436
Score = 42.7 bits (96), Expect = 2e-04
Identities = 20/59 (33%), Positives = 36/59 (61%)
Frame = +1
Query: 358 TSSFGALAHLLKASLGSGVLAMPLAFKNAGLLVGCIGTMLIGLICGHVIHILVKTSQQL 534
+S F A++ A +G+GVL +P AFK G L+G + + + ++ H + +LV T ++L
Sbjct: 34 SSQFKTFANVFIAVVGAGVLGLPYAFKRTGWLMGVLLLVSVSVLTHHCMMLLVYTRRKL 92
>At2g42005.1 68415.m05196 amino acid transporter family protein low
similarity to proton/amino acid transporter 1 [Mus
musculus] GI:21908024; contains Pfam profile PF01490:
Transmembrane amino acid transporter protein
Length = 413
Score = 41.5 bits (93), Expect = 5e-04
Identities = 20/61 (32%), Positives = 34/61 (55%)
Frame = +1
Query: 358 TSSFGALAHLLKASLGSGVLAMPLAFKNAGLLVGCIGTMLIGLICGHVIHILVKTSQQLC 537
+S F A++ A +G+GVL +P AFK G L+G + + + H + +LV ++L
Sbjct: 23 SSKFKTFANVFIAIVGAGVLGLPYAFKRTGWLMGLLTLFSVAALINHCMMLLVHIRRKLG 82
Query: 538 V 540
V
Sbjct: 83 V 83
>At1g80510.1 68414.m09435 amino acid transporter family protein
similar to amino acid transporter system N2 [Rattus
norvegicus] GI:14578932; contains Pfam profile PF01490:
Transmembrane amino acid transporter protein
Length = 489
Score = 39.5 bits (88), Expect = 0.002
Identities = 18/72 (25%), Positives = 40/72 (55%)
Frame = +1
Query: 310 DMGPYVPANHRPMESNTSSFGALAHLLKASLGSGVLAMPLAFKNAGLLVGCIGTMLIGLI 489
D+ Y + + + +GA+ +L + +G+G++A+P K GL++G + +L+ L+
Sbjct: 59 DVADYPLVHGKSSNQGSGIYGAVFNLTTSIIGAGIMALPATMKVLGLVLGFVLIILMALL 118
Query: 490 CGHVIHILVKTS 525
+ +LV+ S
Sbjct: 119 SEISVELLVRFS 130
>At5g38820.1 68418.m04695 amino acid transporter family protein low
similarity to N system amino acids transporter NAT-1
[Mus musculus] GI:7406950; contains Pfam profile
PF01490: Transmembrane amino acid transporter protein
Length = 456
Score = 33.9 bits (74), Expect = 0.10
Identities = 19/60 (31%), Positives = 33/60 (55%), Gaps = 1/60 (1%)
Frame = +1
Query: 349 ESNTSSF-GALAHLLKASLGSGVLAMPLAFKNAGLLVGCIGTMLIGLICGHVIHILVKTS 525
E N +SF GA+ +L +G+G++A+P K GL+ G +L+ + I L++ S
Sbjct: 31 EFNGASFSGAVFNLATTIIGAGIMALPATMKILGLIPGITIIVLMAFLTDASIEFLLRFS 90
>At3g30390.1 68416.m03836 amino acid transporter family protein low
similarity to neuronal glutamine transporter [Rattus
norvegicus] GI:6978016; belongs to INTERPRO:IPR002422
amino acid/polyamine transporter, family II
Length = 460
Score = 32.3 bits (70), Expect = 0.32
Identities = 18/61 (29%), Positives = 34/61 (55%), Gaps = 1/61 (1%)
Frame = +1
Query: 349 ESNTSSF-GALAHLLKASLGSGVLAMPLAFKNAGLLVGCIGTMLIGLICGHVIHILVKTS 525
E N +SF GA+ +L +G+G++A+P K GL +G +++ + I L++ S
Sbjct: 39 EFNGASFSGAVFNLATTIIGAGIMALPATMKILGLGLGITMIVVMAFLTDASIEFLLRFS 98
Query: 526 Q 528
+
Sbjct: 99 K 99
>At2g40420.1 68415.m04985 amino acid transporter family protein
similar to neuronal glutamine transporter [Rattus
norvegicus] GI:6978016; contains Pfam profile PF01490:
Transmembrane amino acid transporter protein
Length = 440
Score = 31.5 bits (68), Expect = 0.55
Identities = 22/73 (30%), Positives = 39/73 (53%), Gaps = 4/73 (5%)
Frame = +1
Query: 319 PYVPANHRPMES---NTSSF-GALAHLLKASLGSGVLAMPLAFKNAGLLVGCIGTMLIGL 486
P +P N P S N SF GA+ ++ + +G+G++A+P AFK G++ ++I
Sbjct: 8 PLLP-NQEPSSSSSENHGSFAGAVFNISTSIVGAGIMAIPAAFKVLGVIPSLSIIVIIAW 66
Query: 487 ICGHVIHILVKTS 525
+ L+K+S
Sbjct: 67 LSNVSAGFLMKSS 79
>At5g41800.1 68418.m05089 amino acid transporter family protein
similar to amino acid permease 1 [Nicotiana sylvestris]
GI:976402; belongs to INTERPRO:IPR002422 amino
acid/polyamine transporter, family II
Length = 452
Score = 30.7 bits (66), Expect = 0.96
Identities = 13/46 (28%), Positives = 25/46 (54%)
Frame = +1
Query: 382 HLLKASLGSGVLAMPLAFKNAGLLVGCIGTMLIGLICGHVIHILVK 519
HL A +G +L +P AF+ G +G + +GL+ + +++ K
Sbjct: 37 HLTTAIVGPTILTLPYAFRGLGWWLGFVCLTTMGLVTFYAYYLMSK 82
>At1g44100.1 68414.m05094 amino acid permease 5, putative (AAP5)
nearly identical to amino acid permease (AAP5) GI:608673
from [Arabidopsis thaliana]
Length = 480
Score = 30.7 bits (66), Expect = 0.96
Identities = 16/50 (32%), Positives = 27/50 (54%)
Frame = +1
Query: 340 RPMESNTSSFGALAHLLKASLGSGVLAMPLAFKNAGLLVGCIGTMLIGLI 489
RP + T + A AH++ A +GSGVL++ A G + G + +L +
Sbjct: 25 RPKRTGTV-WTASAHIITAVIGSGVLSLAWAVAQIGWIGGPVAMLLFSFV 73
>At5g09220.1 68418.m01045 amino acid permease 2 (AAP2) identical to
amine acid permease AAP2 [Arabidopsis thaliana]
GI:510236
Length = 493
Score = 29.9 bits (64), Expect = 1.7
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +1
Query: 373 ALAHLLKASLGSGVLAMPLAFKNAGLLVGCIGTMLIGLI 489
A AH++ A +GSGVL++ A G + G +L L+
Sbjct: 53 ASAHIITAVIGSGVLSLAWAIAQLGWIAGPAVMLLFSLV 91
>At2g39130.1 68415.m04807 amino acid transporter family protein
belongs to INTERPRO:IPR002422 amino acid/polyamine
transporter, family II
Length = 550
Score = 29.9 bits (64), Expect = 1.7
Identities = 24/91 (26%), Positives = 38/91 (41%)
Frame = +1
Query: 217 EKTKPSAPPIQLVMDDNYSSKIELAYNGSREDMGPYVPANHRPMESNTSSFGALAHLLKA 396
E+ A P + S I R+D + ++ PM N+S A+ + L
Sbjct: 113 EQADEQALPKHRLSSQGLLSPIPSRRGSMRKDEKSSMVSHEIPMSRNSSYGQAVLNGLNV 172
Query: 397 SLGSGVLAMPLAFKNAGLLVGCIGTMLIGLI 489
G G+L+ P A K G L G + + GL+
Sbjct: 173 LCGVGILSTPYAAKEGGWL-GLMILFVYGLL 202
>At1g77380.1 68414.m09011 amino acid carrier, putative / amino acid
permease, putative strong similarity to amino acid
carrier GI:3293031 from [Ricinus communis]; contains
Pfam profile PF01490: Transmembrane amino acid
transporter protein; identical to cDNA AAP3 (Amino Acid
Permease) GI:3970651
Length = 476
Score = 29.9 bits (64), Expect = 1.7
Identities = 15/43 (34%), Positives = 24/43 (55%)
Frame = +1
Query: 361 SSFGALAHLLKASLGSGVLAMPLAFKNAGLLVGCIGTMLIGLI 489
S + A AH++ A +GSGVL++ A G L G + +L +
Sbjct: 33 SVWTASAHIITAVIGSGVLSLAWATAQLGWLAGPVVMLLFSAV 75
>At5g24170.1 68418.m02844 expressed protein
Length = 163
Score = 29.1 bits (62), Expect = 2.9
Identities = 15/64 (23%), Positives = 30/64 (46%)
Frame = +1
Query: 355 NTSSFGALAHLLKASLGSGVLAMPLAFKNAGLLVGCIGTMLIGLICGHVIHILVKTSQQL 534
N + G+ A L+ ++ P+ F + +GC+ ++ LIC +IH + T +
Sbjct: 73 NVLAIGSTAFLMGPEQQMSMMFDPVRFLATSIYIGCV---VVALICALLIHSKILTVLAI 129
Query: 535 CVEV 546
E+
Sbjct: 130 LCEI 133
>At1g10010.1 68414.m01129 amino acid permease, putative similar to
amino acid permease I GI:22641 from [Arabidopsis
thaliana]; GC splice site at position 1256 is predicted
from alignment and not confirmed experimentally
Length = 475
Score = 29.1 bits (62), Expect = 2.9
Identities = 15/38 (39%), Positives = 22/38 (57%), Gaps = 1/38 (2%)
Frame = +1
Query: 349 ESNTSSFG-ALAHLLKASLGSGVLAMPLAFKNAGLLVG 459
E T +F A AH++ A +GSGVL++ A G + G
Sbjct: 26 EKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWVAG 63
>At3g56200.1 68416.m06246 amino acid transporter family protein low
similarity to N system amino acids transporter NAT-1
[Mus musculus] GI:7406950; belongs to INTERPRO:IPR002422
amino acid/polyamine transporter, family II
Length = 435
Score = 28.7 bits (61), Expect = 3.9
Identities = 13/56 (23%), Positives = 33/56 (58%)
Frame = +1
Query: 358 TSSFGALAHLLKASLGSGVLAMPLAFKNAGLLVGCIGTMLIGLICGHVIHILVKTS 525
+S+ G + ++ + +G+G+++MP AFK G++ + +I + + L+K++
Sbjct: 23 SSTSGIVFNVSTSIIGAGIMSMPAAFKVLGIVPAFLIITIIAWLSTISVGFLMKST 78
>At5g63850.1 68418.m08015 amino acid transporter 4, putative (AAP4)
identical to amino acid transporter GI:608671 from
[Arabidopsis thaliana];
Length = 466
Score = 27.9 bits (59), Expect = 6.8
Identities = 13/39 (33%), Positives = 21/39 (53%)
Frame = +1
Query: 373 ALAHLLKASLGSGVLAMPLAFKNAGLLVGCIGTMLIGLI 489
A AH++ A +GSGVL++ A G + G +L +
Sbjct: 26 ASAHIITAVIGSGVLSLAWAIGQLGWIAGPTVMLLFSFV 64
>At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein
/ VPS11 family protein similar to Vacuolar protein
sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo
sapiens]; similar to Vacuolar biogenesis protein END1
(PEP5 protein) (Vacuolar protein sorting 11)
(Swiss-Prot:P12868) [Saccharomyces cerevisiae]
Length = 932
Score = 27.9 bits (59), Expect = 6.8
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = -1
Query: 419 ASTPEPRDAFSKWAKAPKLEVFDSIG 342
A TPE + FS A+ PKL+ D IG
Sbjct: 196 AVTPESVNLFSMQAQPPKLQTLDHIG 221
>At1g58360.1 68414.m06638 amino acid permease I (AAP1) identical to
amino acid permease I GI:22641 from [Arabidopsis
thaliana]
Length = 485
Score = 27.9 bits (59), Expect = 6.8
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = +1
Query: 373 ALAHLLKASLGSGVLAMPLAFKNAGLLVG 459
A AH++ A +GSGVL++ A G + G
Sbjct: 44 ASAHIITAVIGSGVLSLAWAIAQLGWIAG 72
>At4g08690.1 68417.m01432 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650: CRAL/TRIO domain; similar to
polyphosphoinositide binding protein Ssh2p (GI:2739046)
[Glycine max]; similar to SEC14-like protein (GB:U82515)
[D. discoideum]
Length = 301
Score = 27.5 bits (58), Expect = 9.0
Identities = 15/58 (25%), Positives = 30/58 (51%), Gaps = 1/58 (1%)
Frame = +1
Query: 238 PPIQLVMDDNYS-SKIELAYNGSREDMGPYVPANHRPMESNTSSFGALAHLLKASLGS 408
P +++M++N+ K+ELA+ G+ + + R E + +L ++ ASL S
Sbjct: 217 PNTKVIMEENFDMEKMELAFGGNDDSGFNIEKHSERMKEDDKKRLASLEGIVSASLDS 274
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,230,715
Number of Sequences: 28952
Number of extensions: 393266
Number of successful extensions: 1053
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 1015
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1053
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1487069504
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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