BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-0723
(625 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC24H6.11c |||sulfate transporter |Schizosaccharomyces pombe|c... 29 0.41
SPAC323.01c |||mitochondrial NADH kinase |Schizosaccharomyces po... 29 0.72
SPCC737.03c |||conserved eukaryotic protein|Schizosaccharomyces ... 27 1.7
SPAC19A8.15 |trp2||tryptophan synthase|Schizosaccharomyces pombe... 25 6.7
SPCC1450.11c |cek1||serine/threonine protein kinase Cek1|Schizos... 25 8.9
SPAC2F7.09c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 8.9
>SPAC24H6.11c |||sulfate transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 958
Score = 29.5 bits (63), Expect = 0.41
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = +3
Query: 129 VPNREEASERSPSPRTVVPKMAHRHQPSEKYCGAISEGKLHTDFLPD 269
+P REE S + P + V + PS+K G IS K++ F D
Sbjct: 105 LPEREEHSSQLPPAQNQVLPFQSLNAPSKKLRGRISLSKIYQFFFDD 151
Score = 25.4 bits (53), Expect = 6.7
Identities = 12/29 (41%), Positives = 15/29 (51%)
Frame = +1
Query: 34 LAYSSTIFPRSPPTHLALFADDTTVYYSS 120
L+Y +FP S P L AD +YY S
Sbjct: 176 LSYGLILFPISDPLFKNLGADGLAIYYVS 204
>SPAC323.01c |||mitochondrial NADH kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 361
Score = 28.7 bits (61), Expect = 0.72
Identities = 23/70 (32%), Positives = 28/70 (40%), Gaps = 1/70 (1%)
Frame = +1
Query: 22 HPSYLAYSST-IFPRSPPTHLALFADDTTVYYSSRNKSLIAKKLQSAALALGQWFRKWRI 198
HPS A T I P S LF D + + NKS + +L LG RI
Sbjct: 261 HPSINALLLTPICPNSLSFRPVLFPDTFKISIETSNKSRVRPQLSIDGRPLGLTDIGQRI 320
Query: 199 DINPAKSTAV 228
DI K A+
Sbjct: 321 DITSVKDNAI 330
>SPCC737.03c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 615
Score = 27.5 bits (58), Expect = 1.7
Identities = 18/64 (28%), Positives = 26/64 (40%)
Frame = +1
Query: 16 SSHPSYLAYSSTIFPRSPPTHLALFADDTTVYYSSRNKSLIAKKLQSAALALGQWFRKWR 195
SSHP Y AY + P + F + Y S L A ++ LG W +K +
Sbjct: 118 SSHPDYQAYEKAL-PEYKKSIEEKFPIVCSECYDSVQDQLDANDYEAKNQVLGYWLQKSK 176
Query: 196 IDIN 207
+N
Sbjct: 177 EQLN 180
>SPAC19A8.15 |trp2||tryptophan synthase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 697
Score = 25.4 bits (53), Expect = 6.7
Identities = 9/19 (47%), Positives = 15/19 (78%)
Frame = +1
Query: 433 PMICKRSKMSLRNKVTLYK 489
P ICK ++++L+N +TL K
Sbjct: 63 PTICKGNEIALKNNITLEK 81
>SPCC1450.11c |cek1||serine/threonine protein kinase
Cek1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1338
Score = 25.0 bits (52), Expect = 8.9
Identities = 12/38 (31%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
Frame = +1
Query: 13 LSSHPSYLAYS-STIFPRSPPTHLALFADDTTVYYSSR 123
+ +HP + + + TI PP F+ + TVY+ SR
Sbjct: 952 IKAHPFFKSVNWDTILEEDPPFVPKPFSPEDTVYFDSR 989
>SPAC2F7.09c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 491
Score = 25.0 bits (52), Expect = 8.9
Identities = 11/36 (30%), Positives = 22/36 (61%)
Frame = +1
Query: 343 LGVTLDASMTFRPHIKSVRDRAAFILGRLYPMICKR 450
LG++ +M + H ++ D+ +LGR+ P++C R
Sbjct: 177 LGISSKYAMLYTSHSFNLVDK---LLGRINPLLCSR 209
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,482,975
Number of Sequences: 5004
Number of extensions: 50468
Number of successful extensions: 130
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 123
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 130
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 275671126
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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