Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ceN-0705
         (705 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe...    66   3e-12
SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyce...    37   0.002
SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam...    36   0.006
SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    28   1.1  
SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces pomb...    27   2.6  
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    27   3.5  
SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor Thi1|Sc...    26   4.6  
SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc...    26   4.6  
SPCC11E10.08 |rik1||silencing protein Rik1|Schizosaccharomyces p...    26   6.0  
SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    26   6.0  
SPBC215.15 |sec13||COPII-coated vesicle component Sec13|Schizosa...    26   6.0  
SPAC4H3.02c |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    26   6.0  
SPCC1672.11c |||P-type ATPase |Schizosaccharomyces pombe|chr 3||...    25   8.0  
SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase Swr1|Schizo...    25   8.0  
SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces ...    25   8.0  
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual    25   8.0  

>SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 560

 Score = 66.5 bits (155), Expect = 3e-12
 Identities = 35/90 (38%), Positives = 52/90 (57%), Gaps = 1/90 (1%)
 Frame = +3

Query: 219 DKGLKTSEDARFYALSRKF-KPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKD 395
           + GL   ++A  +A+S +F +P +   K LVVQ+ V  E+ ++CGG YLK+   +    +
Sbjct: 89  EAGLVMKDEAAHHAISYEFDEPINEPEKDLVVQYEVNPEEGLNCGGAYLKLL-AEPTHGE 147

Query: 396 MHGETPYEIMFGPDICGPGTKKVHVIFSYK 485
           M     Y IMFGPD CG    +VH IF +K
Sbjct: 148 MSNSIDYRIMFGPDKCGV-NDRVHFIFKHK 176



 Score = 58.8 bits (136), Expect = 7e-10
 Identities = 30/57 (52%), Positives = 37/57 (64%), Gaps = 2/57 (3%)
 Frame = +1

Query: 538 THLYTLIVKPDNTYEVLIDNEKVESGDLEADW--DFLSPKKIKDPEAKKPEDWDDKP 702
           T+LYTLIVKPD T+EV I+ + V  G L  D+    L P +I DPE  KP DW D+P
Sbjct: 201 TNLYTLIVKPDQTFEVRINGDVVRQGSLFYDFIPPVLPPVEIYDPEDIKPADWVDEP 257



 Score = 37.9 bits (84), Expect = 0.001
 Identities = 15/26 (57%), Positives = 20/26 (76%), Gaps = 1/26 (3%)
 Frame = +1

Query: 628 DWDFLSPKKIKDPEAKKPEDW-DDKP 702
           DWD  +P+ I DP+A KPEDW +D+P
Sbjct: 269 DWDEDAPRMIPDPDAVKPEDWLEDEP 294



 Score = 37.1 bits (82), Expect = 0.002
 Identities = 15/24 (62%), Positives = 17/24 (70%)
 Frame = +1

Query: 628 DWDFLSPKKIKDPEAKKPEDWDDK 699
           DW    P  I DPEA+KPEDWDD+
Sbjct: 288 DWLEDEPLYIPDPEAQKPEDWDDE 311


>SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 381

 Score = 37.1 bits (82), Expect = 0.002
 Identities = 38/137 (27%), Positives = 57/137 (41%), Gaps = 6/137 (4%)
 Frame = +3

Query: 99  FEEKFPDD---SWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDARFYAL-S 266
           F E+F  +   +W S W       K+ G + L      S   D+ GL T +  + + L S
Sbjct: 33  FSEQFTSEDITTWRSRW--RAPVNKDLGVWDLVEAPG-SHLRDEYGLITLKSNKPHILIS 89

Query: 267 RKFKPFSNEGK--PLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDI 440
               P + +    P+V+ F VK  +   CG  Y+ +   +   K++  E P  I FG   
Sbjct: 90  NLENPTTRQSSSVPIVLSFQVKPTKPWTCGHAYVSLVH-QSNPKNVSKEPPSVIRFGVKK 148

Query: 441 CGPGTKKVHVIFSYKGK 491
           CG        I SY GK
Sbjct: 149 CGMFDYISLSIISYDGK 165


>SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein
           Mam3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1082

 Score = 35.9 bits (79), Expect = 0.006
 Identities = 26/72 (36%), Positives = 39/72 (54%), Gaps = 1/72 (1%)
 Frame = -3

Query: 253 NLASSEVFKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHE-SSGNFSSKNTSQFIE 77
           N  SSE F   S+S SL   P VN +   S    + ++ L S + S+ NF+S ++S  + 
Sbjct: 475 NYTSSEAF---STSSSLVVIPPVNSSLVTSSTSFTKFSSLSSSQLSTENFTSASSSLSLT 531

Query: 76  DNASKLTTTSTT 41
           +  S L+T STT
Sbjct: 532 NAKSSLSTPSTT 543


>SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 821

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 15/44 (34%), Positives = 19/44 (43%)
 Frame = -3

Query: 184 NLNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQFIEDNASKLTT 53
           N N    F      + + S  +SGNFS   T Q   DN S  +T
Sbjct: 575 NSNSQRPFSTVPSESNVFSRNASGNFSMSQTHQPTTDNTSSFST 618


>SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1254

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 19/70 (27%), Positives = 37/70 (52%), Gaps = 5/70 (7%)
 Frame = +1

Query: 511 DIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDL-EADWDFLSPKKIKDP----EAK 675
           D   K   +  L T I + ++ +   + + K+E+ +L E D  F++ ++ + P    E+ 
Sbjct: 148 DSPTKKKSFKSLDTSIFQAEDQFRHPVSS-KLENSELSEVDKPFIASRRSRKPVSYAESD 206

Query: 676 KPEDWDDKPT 705
           + ED+DD PT
Sbjct: 207 EDEDFDDAPT 216


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 26.6 bits (56), Expect = 3.5
 Identities = 33/137 (24%), Positives = 59/137 (43%)
 Frame = -3

Query: 445 PQMSGPNIISYGVSPCMSFCSSLQSKTLRYPPPQSMSCSCLTVNWTTKGLPSLLNGLNLR 266
           P  S   I S   SP  S  SS  S +       S S +  + + T+    +  +  + R
Sbjct: 336 PTSSSSTISSSSSSPSSSSFSSTTSSS---KSSSSFSSTVSSSSSTSSSTLTSSSSSSSR 392

Query: 265 ERA*NLASSEVFKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQ 86
             + +  SS +    SSS S  +   V+  F ++    S  ++  SH SS + SS ++  
Sbjct: 393 PASSSSHSSSLSSHKSSSSSKSSSAPVSSAFYHN----STSSRSSSHSSSHSLSSLSSKP 448

Query: 85  FIEDNASKLTTTSTTAF 35
            +  ++S L T+S+  +
Sbjct: 449 ILTASSSSLLTSSSHTY 465


>SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor
           Thi1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 775

 Score = 26.2 bits (55), Expect = 4.6
 Identities = 10/32 (31%), Positives = 18/32 (56%)
 Frame = -3

Query: 253 NLASSEVFKPLSSSGSLKNFPAVNLNFPNSFP 158
           N   +E F+P   + SL N P +N ++ ++ P
Sbjct: 685 NSTPNEAFQPFDQTSSLYNVPGLNKSYVSNQP 716


>SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1190

 Score = 26.2 bits (55), Expect = 4.6
 Identities = 12/48 (25%), Positives = 24/48 (50%)
 Frame = +3

Query: 105 EKFPDDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDA 248
           + F +D  + +W   EH  K   ++   + + FS+  DD+  + SE +
Sbjct: 49  QAFQEDEGDEDWEEEEHKPKAKRRYNTRSNESFSE-GDDEPFEVSESS 95


>SPCC11E10.08 |rik1||silencing protein Rik1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1040

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 18/58 (31%), Positives = 31/58 (53%), Gaps = 4/58 (6%)
 Frame = -3

Query: 277 LNLRERA*NLASSEV----FKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHESS 116
           ++LR RA  L+  E+     + LS+   LK   +++  FP SFP   +Y   + ++SS
Sbjct: 121 VDLRMRAIFLSIDEISMICIRILSAEERLKTGRSIDSGFPFSFPVHLIYDMCILNDSS 178


>SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 536

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 27/123 (21%), Positives = 53/123 (43%), Gaps = 2/123 (1%)
 Frame = -3

Query: 385 SSLQSKTLRYPPPQSMSCSCLTVNWTTKGLPSLLNGLNLRERA*NLASSEVFKPLSSSG- 209
           +SL S      P  S S +  T   ++  +PS  + +     A  ++SS    PLSSS  
Sbjct: 12  TSLSSSASSSIPASSSSAAASTSLSSSSVIPSSSSSMLSSSSATAISSSSSSSPLSSSSF 71

Query: 208 -SLKNFPAVNLNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQFIEDNASKLTTTSTTAFI 32
            S  +   +     +S    S  +  L+  SS   +S +++     ++S   ++S+++ +
Sbjct: 72  TSPASSSFITSLVSSSSQQSSSSSASLTSSSSATLTSSSSASPTSSSSSHALSSSSSSLV 131

Query: 31  FDS 23
             S
Sbjct: 132 ASS 134


>SPBC215.15 |sec13||COPII-coated vesicle component
           Sec13|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 297

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 9/16 (56%), Positives = 11/16 (68%)
 Frame = +3

Query: 102 EEKFPDDSWESNWVYS 149
           EEKFPD +W  +W  S
Sbjct: 251 EEKFPDIAWRVSWSLS 266


>SPAC4H3.02c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 391

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 12/32 (37%), Positives = 16/32 (50%)
 Frame = +1

Query: 607 ESGDLEADWDFLSPKKIKDPEAKKPEDWDDKP 702
           E   L+     L   + K P AKKPED++  P
Sbjct: 157 EKNTLDLKHCLLPSPEYKAPPAKKPEDYEASP 188


>SPCC1672.11c |||P-type ATPase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1315

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 12/31 (38%), Positives = 14/31 (45%)
 Frame = -1

Query: 147 CTPNCFPTNHQETSPQRIHHNLSKIMPAS*Q 55
           C P  FP N+QE      H+    I  AS Q
Sbjct: 791 CKPESFPANYQEVLDYYTHNGFRVIACASKQ 821


>SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase
            Swr1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1288

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 18/58 (31%), Positives = 23/58 (39%)
 Frame = +3

Query: 231  KTSEDARFYALSRKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHG 404
            KT      Y +SRK  P       L + F  K     DC  G L+V D  L+    +G
Sbjct: 952  KTIPRDLLYEVSRKINPLHQASTRLAIAFPDKRLLQYDC--GKLQVLDRLLKDLVSNG 1007


>SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1364

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 14/35 (40%), Positives = 17/35 (48%)
 Frame = +3

Query: 189 AGKFFSDPEDDKGLKTSEDARFYALSRKFKPFSNE 293
           A  F S  E  KGL +SE    Y+L     P SN+
Sbjct: 263 ADSFLSHSESIKGLSSSEQGTVYSLKASHDP-SNQ 296


>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1236

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 30/145 (20%), Positives = 58/145 (40%), Gaps = 1/145 (0%)
 Frame = -3

Query: 454 VPGPQMSGPNIISYGVSPCMSFCSSLQSKTLRYPPPQSMSCSCLTVNWTTKGLPSLLNGL 275
           +P    S P+  S   SP  S  SS  S         S S + ++   ++  +PS  + +
Sbjct: 518 IPSTSSSTPSS-SIISSPMTSVLSSSSSIPTSSSSDFSSSITTISSGISSSSIPSTFSSV 576

Query: 274 NLRERA*NLASSEVFKPLSSSGSLKNFPAVNLNFPNSF-PGCSLYTQLLSHESSGNFSSK 98
           +    +   + S     +SSS +   F + + + P+S     S  + +LS  +    S  
Sbjct: 577 SSILSSSTSSPSSTSLSISSSSTSSTFSSASTSSPSSISSSISSSSTILSSPTPSTSSLM 636

Query: 97  NTSQFIEDNASKLTTTSTTAFIFDS 23
            +S  I   +S + ++S +     S
Sbjct: 637 ISSSSIISGSSSILSSSISTIPISS 661


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,095,887
Number of Sequences: 5004
Number of extensions: 68592
Number of successful extensions: 244
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 231
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 242
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 327172622
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)