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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ceN-0705
         (705 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_30637| Best HMM Match : No HMM Matches (HMM E-Value=.)             134   7e-32
SB_49315| Best HMM Match : Calreticulin (HMM E-Value=0)                67   1e-11
SB_29195| Best HMM Match : No HMM Matches (HMM E-Value=.)              40   0.002
SB_50300| Best HMM Match : No HMM Matches (HMM E-Value=.)              31   0.91 
SB_48987| Best HMM Match : Ank (HMM E-Value=2.7e-36)                   29   2.8  
SB_28048| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   4.9  
SB_5367| Best HMM Match : wnt (HMM E-Value=0)                          29   4.9  
SB_15074| Best HMM Match : PID (HMM E-Value=0.00012)                   28   6.4  
SB_59294| Best HMM Match : PI3_PI4_kinase (HMM E-Value=2.20004e-41)    28   6.4  
SB_10980| Best HMM Match : PID (HMM E-Value=0.00012)                   28   6.4  

>SB_30637| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1137

 Score =  134 bits (324), Expect = 7e-32
 Identities = 61/92 (66%), Positives = 71/92 (77%), Gaps = 1/92 (1%)
 Frame = +3

Query: 93   VFFEEKFPDDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDARFYALSRK 272
            V F EKF D SWE  WV S   G + GKFK TAGKF+ D E DKG++TSEDA+FY +S K
Sbjct: 756  VHFLEKFEDKSWEDRWVSSTSKGAQQGKFKWTAGKFYGDAEADKGIQTSEDAKFYGISAK 815

Query: 273  F-KPFSNEGKPLVVQFTVKHEQDIDCGGGYLK 365
            F KPF+NEGK LV+QF+VKHEQ+IDCGGGY K
Sbjct: 816  FEKPFTNEGKTLVIQFSVKHEQNIDCGGGYAK 847



 Score =  116 bits (280), Expect = 1e-26
 Identities = 51/59 (86%), Positives = 53/59 (89%)
 Frame = +1

Query: 523  KDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEADWDFLSPKKIKDPEAKKPEDWDDK 699
            KDD  THLYTLIV+PDNTYEV IDNEKVESG+LE DWDFL PK IKDPEAKKPEDWDDK
Sbjct: 847  KDDEMTHLYTLIVRPDNTYEVKIDNEKVESGELEKDWDFLPPKTIKDPEAKKPEDWDDK 905



 Score = 41.1 bits (92), Expect = 9e-04
 Identities = 16/24 (66%), Positives = 19/24 (79%)
 Frame = +1

Query: 628 DWDFLSPKKIKDPEAKKPEDWDDK 699
           DWD   P+ I DP+AKKPEDWDD+
Sbjct: 918 DWD--KPQNIPDPDAKKPEDWDDE 939


>SB_49315| Best HMM Match : Calreticulin (HMM E-Value=0)
          Length = 1086

 Score = 67.3 bits (157), Expect = 1e-11
 Identities = 30/60 (50%), Positives = 40/60 (66%), Gaps = 2/60 (3%)
 Frame = +1

Query: 526 DDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEADW--DFLSPKKIKDPEAKKPEDWDDK 699
           D   THL+TL+V+PDNT+EV ID E V  G L  D       P +I+DP+ K+PEDWD++
Sbjct: 87  DGKKTHLFTLVVRPDNTFEVFIDQESVNKGSLLEDMTPPVNPPAEIEDPDDKRPEDWDER 146



 Score = 45.2 bits (102), Expect = 5e-05
 Identities = 22/48 (45%), Positives = 31/48 (64%), Gaps = 1/48 (2%)
 Frame = +3

Query: 375 CKLEQKDMHGETPYEIMFGPDICGPGTKKVHVIFSYKG-KNHLIKKRY 515
           C L Q+    +TPY IMFGPD CG   +K+H IF +K  KN  I++++
Sbjct: 28  CHLFQESFGDKTPYTIMFGPDKCGE-DRKLHFIFRHKNPKNGTIEEKH 74



 Score = 39.5 bits (88), Expect = 0.003
 Identities = 20/40 (50%), Positives = 24/40 (60%), Gaps = 3/40 (7%)
 Frame = +1

Query: 592 DNEKVESGDLEA--DWDFLSPKKIKDPEAKKPEDW-DDKP 702
           + EK+   D E   DWD  +P KI D  AKKPE W DD+P
Sbjct: 145 EREKIPDPDAEKPDDWDEDAPVKIPDTNAKKPEGWLDDEP 184



 Score = 30.3 bits (65), Expect = 1.6
 Identities = 10/18 (55%), Positives = 13/18 (72%)
 Frame = +1

Query: 646 PKKIKDPEAKKPEDWDDK 699
           P+ + DP   KPEDWDD+
Sbjct: 184 PEYVPDPNGIKPEDWDDE 201


>SB_29195| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 539

 Score = 39.9 bits (89), Expect = 0.002
 Identities = 30/98 (30%), Positives = 44/98 (44%), Gaps = 3/98 (3%)
 Frame = -3

Query: 352 PPQSMSCSCLTVNWTTKGLPSLLNG---LNLRERA*NLASSEVFKPLSSSGSLKNFPAVN 182
           P +  SC CL V W T GLP L++G   L    R   L+   +  P   S SL +   V 
Sbjct: 429 PSEQNSCQCLFVGWQTVGLPQLIHGTVALISFRRKRTLSGGSL--PRGRSRSLSS-DRVG 485

Query: 181 LNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQFIEDNA 68
              P++  G +    +    S+G+  S+ +SQ   D A
Sbjct: 486 TRLPSARKGSASKIGIPRTSSTGSLGSRRSSQSSTDGA 523


>SB_50300| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 3669

 Score = 31.1 bits (67), Expect = 0.91
 Identities = 15/40 (37%), Positives = 23/40 (57%)
 Frame = -3

Query: 313  WTTKGLPSLLNGLNLRERA*NLASSEVFKPLSSSGSLKNF 194
            +TT+GLPS ++ +NL E   NL S    KP   S  + ++
Sbjct: 959  YTTQGLPSKISSVNLTEALSNLISISWSKPSDGSSLITDY 998


>SB_48987| Best HMM Match : Ank (HMM E-Value=2.7e-36)
          Length = 551

 Score = 29.5 bits (63), Expect = 2.8
 Identities = 16/42 (38%), Positives = 21/42 (50%)
 Frame = +1

Query: 511 DIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEADWD 636
           D+ CKDD         V  D T E L+D+ +V+S  L  D D
Sbjct: 485 DVSCKDDKNRTALHWAVGQDKTIEGLLDDPRVQSLQLVNDQD 526


>SB_28048| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 125

 Score = 28.7 bits (61), Expect = 4.9
 Identities = 10/22 (45%), Positives = 17/22 (77%)
 Frame = -2

Query: 155 VLTVHPIAFPRIIRKLLLKEYI 90
           +LT H +  P++IRK+ LK+Y+
Sbjct: 73  LLTAHLVVAPKLIRKMPLKDYV 94


>SB_5367| Best HMM Match : wnt (HMM E-Value=0)
          Length = 367

 Score = 28.7 bits (61), Expect = 4.9
 Identities = 9/33 (27%), Positives = 17/33 (51%)
 Frame = -3

Query: 616 RQIQLSHCQ*GLHRCCQVSQSECTNVCRHHLCS 518
           +++Q + C+   H CC+V    C      H+C+
Sbjct: 335 KEVQATRCRCKFHWCCKVKCKTCIKNVTTHICN 367


>SB_15074| Best HMM Match : PID (HMM E-Value=0.00012)
          Length = 1153

 Score = 28.3 bits (60), Expect = 6.4
 Identities = 15/45 (33%), Positives = 26/45 (57%), Gaps = 1/45 (2%)
 Frame = -2

Query: 524 LQRISFFDEMVLTL-VTEDYVYLLGSRTTNVRAEHNLIWSLSVHV 393
           L ++S  D   +TL +  D + ++  RT++V   HN+ W LS+ V
Sbjct: 766 LDKLSPRDYRPITLKIRPDEIVIVDERTSSVLHTHNVAWVLSLGV 810


>SB_59294| Best HMM Match : PI3_PI4_kinase (HMM E-Value=2.20004e-41)
          Length = 851

 Score = 28.3 bits (60), Expect = 6.4
 Identities = 14/34 (41%), Positives = 21/34 (61%)
 Frame = +1

Query: 556 IVKPDNTYEVLIDNEKVESGDLEADWDFLSPKKI 657
           ++K + T EV  + E V++ D E  WD +SP KI
Sbjct: 677 LIKYEKTPEV--ETETVKTADGETAWDVVSPPKI 708


>SB_10980| Best HMM Match : PID (HMM E-Value=0.00012)
          Length = 463

 Score = 28.3 bits (60), Expect = 6.4
 Identities = 15/45 (33%), Positives = 26/45 (57%), Gaps = 1/45 (2%)
 Frame = -2

Query: 524 LQRISFFDEMVLTL-VTEDYVYLLGSRTTNVRAEHNLIWSLSVHV 393
           L ++S  D   +TL +  D + ++  RT++V   HN+ W LS+ V
Sbjct: 96  LDKLSPRDYRPITLKIRPDEIVIVDERTSSVLHTHNVAWVLSLGV 140


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 23,141,983
Number of Sequences: 59808
Number of extensions: 532249
Number of successful extensions: 1380
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1264
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1374
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1853669818
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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