Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-0661
(661 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q6P2Q9 Cluster: Pre-mRNA-processing-splicing factor 8; ... 189 6e-47
UniRef50_A6R4K4 Cluster: Pre-mRNA processing splicing factor 8; ... 173 3e-42
UniRef50_Q8I1X5 Cluster: Pre-mRNA splicing factor, putative; n=1... 89 4e-30
UniRef50_A4IBT3 Cluster: PRP8 protein homologue, putative; n=8; ... 94 2e-18
UniRef50_Q9AW36 Cluster: Splicing factor Prp8; n=1; Guillardia t... 79 7e-14
UniRef50_A7Q5A9 Cluster: Chromosome undetermined scaffold_52, wh... 49 1e-04
UniRef50_A5C0J0 Cluster: Putative uncharacterized protein; n=1; ... 40 0.053
UniRef50_A5E0E9 Cluster: Putative uncharacterized protein; n=1; ... 33 6.1
UniRef50_Q4PIF4 Cluster: Putative uncharacterized protein; n=1; ... 33 8.0
>UniRef50_Q6P2Q9 Cluster: Pre-mRNA-processing-splicing factor 8;
n=106; Eukaryota|Rep: Pre-mRNA-processing-splicing factor
8 - Homo sapiens (Human)
Length = 2335
Score = 189 bits (460), Expect = 6e-47
Identities = 115/213 (53%), Positives = 131/213 (61%), Gaps = 5/213 (2%)
Frame = +1
Query: 37 PRKSYKMNSSCADILLFSAYKWNVSRPSLLADTKDTMDNTTTQKYWLDIQVGWGDYDSHD 216
PRKSYKMNSSCADILLF++YKWNVSRPSLLAD+KD MD+TTTQKYW+DIQ+ WGDYDSHD
Sbjct: 1616 PRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHD 1675
Query: 217 IERYARAKFW-ITLQTTCLYIFTYRFADRH*FGL*LAQCIR*LVPRLQTSY*QAMAKIMK 393
IERYARAKF T +Y L P + QAMAKIMK
Sbjct: 1676 IERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMK 1735
Query: 394 ANPALYVLRERIRKALQLYS*NLLNLTFPVRTMENCSXIKSFGSLM----TRMCIV*QLQ 561
ANPALYVLRERIRK LQLYS T P + +N + S + T + V +
Sbjct: 1736 ANPALYVLRERIRKGLQLYS---SEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHK 1792
Query: 562 NL*RKLDN*THQRSHFLYFNPRTGQLFLKIIHT 660
L + F+ FNPRTGQLFLKIIHT
Sbjct: 1793 TFEGNLTTKPINGAIFI-FNPRTGQLFLKIIHT 1824
Score = 84.6 bits (200), Expect = 2e-15
Identities = 57/129 (44%), Positives = 69/129 (53%), Gaps = 4/129 (3%)
Frame = +3
Query: 243 LDYTTDNMSIYLHLPVC*SPLIW-PITCTVHTVTGS--PVAN-LLLTSDGQDNES*SCLV 410
LDYTTDNMSIY P LI + +H+ G+ P + L+ + + ++ L
Sbjct: 1685 LDYTTDNMSIY---PSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALY 1741
Query: 411 CASGTYT*STAIVFLEPTEPYLSSQNYGELFXNQIIWFVDDTNVYRVTITKPLKET*QLN 590
+ EPTEPYLSSQNYGELF NQIIWFVDDTNVYRVTI K +
Sbjct: 1742 VLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTK 1801
Query: 591 PSTEPFFIF 617
P FIF
Sbjct: 1802 PINGAIFIF 1810
Score = 84.6 bits (200), Expect = 2e-15
Identities = 53/113 (46%), Positives = 66/113 (58%), Gaps = 2/113 (1%)
Frame = +2
Query: 278 SPTGLLIAIDLAYNLHSAYGNWFPGCKPLINKRWPR**KLILPCMCFGNVYVKHCNCILR 457
SPTG+LIAIDLAYNLHSAYGNWFPG KPLI + + K P + ++ +
Sbjct: 1697 SPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMK-ANPALYVLRERIRKGLQLYS 1755
Query: 458 TY*TLPFQSELWRIVL*SNHLVR**HE--CVSCNNYKTFEGNLTTKPINGAIF 610
+ T P+ S L SN ++ + +KTFEGNLTTKPINGAIF
Sbjct: 1756 SEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIF 1808
>UniRef50_A6R4K4 Cluster: Pre-mRNA processing splicing factor 8; n=1;
Ajellomyces capsulatus NAm1|Rep: Pre-mRNA processing
splicing factor 8 - Ajellomyces capsulatus NAm1
Length = 2739
Score = 173 bits (421), Expect = 3e-42
Identities = 111/217 (51%), Positives = 130/217 (59%), Gaps = 9/217 (4%)
Frame = +1
Query: 37 PRKSYKMNSSCADILLFSAYKWNVSRPSLLADTKDTMDNTTTQKYWLDIQVGWGDYDSHD 216
PRKSYKMNSSCADILLF+ +KWNV+RPSLL DTKD ++ TTT K+WLD+Q+ +GDYDSHD
Sbjct: 2019 PRKSYKMNSSCADILLFATHKWNVTRPSLLFDTKDVIEPTTTNKFWLDVQLRYGDYDSHD 2078
Query: 217 IERYARAKFWITLQTTCLYIFTYRFADRH*FGL*LA----QCIR*LVPRLQTSY*QAMAK 384
IERY RAK+ L T + Y A G+ LA P L+ QAMAK
Sbjct: 2079 IERYVRAKY---LDYTTDSMSIYPSATGLMIGIDLAYKPYSAYGQYFPGLKALVQQAMAK 2135
Query: 385 IMKANPALYVLRERIRKALQLY----S*NLLNLTFPVRTMENCSXIKSFGSLMTRMCIV* 552
IMKANPALYVLRERIRK LQLY + LN N + + + R+ I
Sbjct: 2136 IMKANPALYVLRERIRKGLQLYASESNQEFLNSQNYSELFSNKIQLFIDDTNVYRVTIHK 2195
Query: 553 QLQ-NL*RKLDN*THQRSHFLYFNPRTGQLFLKIIHT 660
+ NL K N FNPRTGQLFLKIIHT
Sbjct: 2196 TFEGNLTTKPIN-----GAIFIFNPRTGQLFLKIIHT 2227
Score = 56.0 bits (129), Expect = 8e-07
Identities = 27/54 (50%), Positives = 33/54 (61%)
Frame = +3
Query: 456 EPTEPYLSSQNYGELFXNQIIWFVDDTNVYRVTITKPLKET*QLNPSTEPFFIF 617
E + +L+SQNY ELF N+I F+DDTNVYRVTI K + P FIF
Sbjct: 2160 ESNQEFLNSQNYSELFSNKIQLFIDDTNVYRVTIHKTFEGNLTTKPINGAIFIF 2213
>UniRef50_Q8I1X5 Cluster: Pre-mRNA splicing factor, putative; n=1;
Plasmodium falciparum 3D7|Rep: Pre-mRNA splicing factor,
putative - Plasmodium falciparum (isolate 3D7)
Length = 3136
Score = 89.4 bits (212), Expect(2) = 4e-30
Identities = 65/173 (37%), Positives = 90/173 (52%), Gaps = 5/173 (2%)
Frame = +1
Query: 157 TTQKYWLDIQVGWGDYDSHDIERYARAKFW-ITLQTTCLYIFTYRFADRH*FGL*LAQCI 333
T+ ++W+DIQ+ WGD+DSHDIERY+RAKF T +Y L
Sbjct: 2353 TSNQFWIDIQLRWGDFDSHDIERYSRAKFLDYTTDNLSIYPCLTGVLIGVDLAYNLYSAY 2412
Query: 334 R*LVPRLQTSY*QAMAKIMKANPALYVLRERIRKALQLYS*NLLNLTFPVRTMENCSXIK 513
L+ +A+ KI+++NP+LYVLRERIRK LQLYS T P +N + +
Sbjct: 2413 GNWFNNLKPLMQKALQKIVQSNPSLYVLRERIRKGLQLYS---SEPTEPYLNTQNYNELF 2469
Query: 514 SFGSLM----TRMCIV*QLQNL*RKLDN*THQRSHFLYFNPRTGQLFLKIIHT 660
S ++ T + V + L + F+ NP+TGQLFLKIIHT
Sbjct: 2470 SSQTIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFI-LNPKTGQLFLKIIHT 2521
Score = 72.9 bits (171), Expect = 6e-12
Identities = 52/128 (40%), Positives = 65/128 (50%), Gaps = 4/128 (3%)
Frame = +3
Query: 243 LDYTTDNMSIYLHLPVC*SPLIW-PITCTVHTVTGSPVANL--LLTSDGQDN-ES*SCLV 410
LDYTTDN+SIY P LI + +++ G+ NL L+ Q +S L
Sbjct: 2382 LDYTTDNLSIY---PCLTGVLIGVDLAYNLYSAYGNWFNNLKPLMQKALQKIVQSNPSLY 2438
Query: 411 CASGTYT*STAIVFLEPTEPYLSSQNYGELFXNQIIWFVDDTNVYRVTITKPLKET*QLN 590
+ EPTEPYL++QNY ELF +Q IWFVDDTNVYRVTI K +
Sbjct: 2439 VLRERIRKGLQLYSSEPTEPYLNTQNYNELFSSQTIWFVDDTNVYRVTIHKTFEGNLTTK 2498
Query: 591 PSTEPFFI 614
P FI
Sbjct: 2499 PINGAIFI 2506
Score = 64.9 bits (151), Expect(2) = 4e-30
Identities = 28/41 (68%), Positives = 31/41 (75%)
Frame = +1
Query: 37 PRKSYKMNSSCADILLFSAYKWNVSRPSLLADTKDTMDNTT 159
PRKSYKMNSSCADILLF+ YKW +S+PSLL D N T
Sbjct: 2272 PRKSYKMNSSCADILLFANYKWGISKPSLLTDEDHIFTNNT 2312
Score = 52.0 bits (119), Expect = 1e-05
Identities = 21/30 (70%), Positives = 25/30 (83%)
Frame = +2
Query: 284 TGLLIAIDLAYNLHSAYGNWFPGCKPLINK 373
TG+LI +DLAYNL+SAYGNWF KPL+ K
Sbjct: 2396 TGVLIGVDLAYNLYSAYGNWFNNLKPLMQK 2425
Score = 41.9 bits (94), Expect = 0.013
Identities = 20/31 (64%), Positives = 24/31 (77%)
Frame = +2
Query: 557 YKTFEGNLTTKPINGAIFYISIPELDSYSLK 649
+KTFEGNLTTKPINGAIF ++ P+ LK
Sbjct: 2488 HKTFEGNLTTKPINGAIFILN-PKTGQLFLK 2517
>UniRef50_A4IBT3 Cluster: PRP8 protein homologue, putative; n=8;
Trypanosomatidae|Rep: PRP8 protein homologue, putative -
Leishmania infantum
Length = 2427
Score = 94.3 bits (224), Expect = 2e-18
Identities = 53/141 (37%), Positives = 79/141 (56%), Gaps = 2/141 (1%)
Frame = +1
Query: 37 PRKSYKMNSSCADILLFSAYKWNVS-RPSLLAD-TKDTMDNTTTQKYWLDIQVGWGDYDS 210
P+KSY SS AD++L SA +W+VS +P+ LAD D ++TT K+W+D+Q+ WG+YDS
Sbjct: 1690 PQKSYTFTSSAADVILVSAARWSVSSKPTSLADEVGDVYQHSTTSKFWIDVQLRWGNYDS 1749
Query: 211 HDIERYARAKFWITLQTTCLYIFTYRFADRH*FGL*LAQCIR*LVPRLQTSY*QAMAKIM 390
H+I YAR KF+ T +Y F +P ++ + M IM
Sbjct: 1750 HNIAEYARKKFY-EYSTARMYPFPAGIVVAIDLAYNCHSAFGYWIPGMKPFMSKLMPVIM 1808
Query: 391 KANPALYVLRERIRKALQLYS 453
K N L LR+R+++ L L+S
Sbjct: 1809 KNNITLNTLRDRMKRDLGLFS 1829
>UniRef50_Q9AW36 Cluster: Splicing factor Prp8; n=1; Guillardia
theta|Rep: Splicing factor Prp8 - Guillardia theta
(Cryptomonas phi)
Length = 2057
Score = 79.4 bits (187), Expect = 7e-14
Identities = 61/216 (28%), Positives = 101/216 (46%), Gaps = 5/216 (2%)
Frame = +1
Query: 16 QKGNHAMPRKSYKMNSSCADILLFSAYKWNVSRPSLLADTKDTMDNT---TTQKYWLDIQ 186
+K NH P+KSYK SS AD++L+ + ++ P LL K +Q YW+D+Q
Sbjct: 1428 KKVNH--PKKSYKFESSSADLILYPKINFLITYPILLGIKKILTHGDFLKVSQVYWIDVQ 1485
Query: 187 VGWGDYDSHDIERYARAKFW-ITLQTTCLYIFTYRFADRH*FGL*LAQCIR*LVPRLQTS 363
+ WGD+DSHDIERY R K++ LY + + + + L
Sbjct: 1486 LRWGDFDSHDIERYVRMKYYEYNDVKKKLYPSGHGILIAYDLCYNVYSSYGNWILGLSNF 1545
Query: 364 Y*QAMAKIMKANPALYVLRERIRKALQLYS*NLLNLTFPVRTMENCSXIKSFGSLMTRMC 543
+ K + L +LR RIRK+LQ+Y N + + +++ K ++ C
Sbjct: 1546 IKNELFSFHKNSAILNILRSRIRKSLQIYQKNNIESNESILNIDD--FFKKKCLIVDDSC 1603
Query: 544 IV*QLQNL*RKLDN*THQRSHFLY-FNPRTGQLFLK 648
+ L+ + + + S FL+ FNP G +++K
Sbjct: 1604 LSNHLELQNLQKNKVINYHSGFLFIFNPINGLIYIK 1639
Score = 37.9 bits (84), Expect = 0.21
Identities = 15/27 (55%), Positives = 19/27 (70%)
Frame = +2
Query: 287 GLLIAIDLAYNLHSAYGNWFPGCKPLI 367
G+LIA DL YN++S+YGNW G I
Sbjct: 1520 GILIAYDLCYNVYSSYGNWILGLSNFI 1546
>UniRef50_A7Q5A9 Cluster: Chromosome undetermined scaffold_52, whole
genome shotgun sequence; n=1; Vitis vinifera|Rep:
Chromosome undetermined scaffold_52, whole genome
shotgun sequence - Vitis vinifera (Grape)
Length = 97
Score = 48.8 bits (111), Expect = 1e-04
Identities = 20/33 (60%), Positives = 27/33 (81%)
Frame = +1
Query: 40 RKSYKMNSSCADILLFSAYKWNVSRPSLLADTK 138
RK YKMNSSC+D LLF+A+KW++ +PSL + K
Sbjct: 39 RKRYKMNSSCSDTLLFAAHKWSMPKPSLDPEPK 71
>UniRef50_A5C0J0 Cluster: Putative uncharacterized protein; n=1;
Vitis vinifera|Rep: Putative uncharacterized protein -
Vitis vinifera (Grape)
Length = 195
Score = 39.9 bits (89), Expect = 0.053
Identities = 17/19 (89%), Positives = 18/19 (94%)
Frame = +1
Query: 37 PRKSYKMNSSCADILLFSA 93
PRKSYKMNSSCAD LLF+A
Sbjct: 26 PRKSYKMNSSCADTLLFAA 44
>UniRef50_A5E0E9 Cluster: Putative uncharacterized protein; n=1;
Lodderomyces elongisporus NRRL YB-4239|Rep: Putative
uncharacterized protein - Lodderomyces elongisporus
(Yeast) (Saccharomyces elongisporus)
Length = 150
Score = 33.1 bits (72), Expect = 6.1
Identities = 16/37 (43%), Positives = 23/37 (62%)
Frame = -3
Query: 569 QRFCNCYTIHIRVINEPNDLIXEQFSIVLTGKVRFSR 459
Q F N T+HI VIN+ ++ EQF I++ + FSR
Sbjct: 97 QCFMNSDTVHICVINKQLRIVVEQFGIIIRIQESFSR 133
>UniRef50_Q4PIF4 Cluster: Putative uncharacterized protein; n=1;
Ustilago maydis|Rep: Putative uncharacterized protein -
Ustilago maydis (Smut fungus)
Length = 616
Score = 32.7 bits (71), Expect = 8.0
Identities = 14/37 (37%), Positives = 18/37 (48%)
Frame = +1
Query: 130 DTKDTMDNTTTQKYWLDIQVGWGDYDSHDIERYARAK 240
D + +D TT WL +Q GWG H +R A K
Sbjct: 342 DLLELLDYPTTAYSWLGVQHGWGGTSGHPRDRLAEEK 378
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 709,368,964
Number of Sequences: 1657284
Number of extensions: 14907130
Number of successful extensions: 31712
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 30662
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 31698
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 50000004659
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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