Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-0661
(661 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g80070.1 68414.m09373 splicing factor, putative strong simila... 163 7e-41
At4g38780.1 68417.m05491 splicing factor, putative strong simila... 162 2e-40
At5g59520.1 68418.m07459 zinc transporter (ZIP2) identical to zi... 29 2.1
At5g10520.1 68418.m01218 protein kinase family protein contains ... 28 4.8
At5g15170.1 68418.m01777 tyrosyl-DNA phosphodiesterase-related s... 28 6.3
At5g45380.1 68418.m05577 sodium:solute symporter family protein ... 27 8.4
At5g01620.2 68418.m00078 expressed protein several hypothetical ... 27 8.4
At5g01620.1 68418.m00077 expressed protein several hypothetical ... 27 8.4
At1g21640.1 68414.m02709 ATP-NAD kinase family protein contains ... 27 8.4
>At1g80070.1 68414.m09373 splicing factor, putative strong similarity
to splicing factor Prp8 [Homo sapiens] GI:3661610;
contains Pfam profile PF01398: Mov34/MPN/PAD-1 family
Length = 2382
Score = 163 bits (397), Expect = 7e-41
Identities = 100/213 (46%), Positives = 123/213 (57%), Gaps = 5/213 (2%)
Frame = +1
Query: 37 PRKSYKMNSSCADILLFSAYKWNVSRPSLLADTKDTMDNTTTQKYWLDIQVGWGDYDSHD 216
PRKSYKMNSSCAD+LLF+A+KW +S+PSL+A++KD D + KYW+D+Q+ WGDYDSHD
Sbjct: 1663 PRKSYKMNSSCADVLLFAAHKWPMSKPSLVAESKDMFDQKASNKYWIDVQLRWGDYDSHD 1722
Query: 217 IERYARAKFW-ITLQTTCLYIFTYRFADRH*FGL*LAQCIR*LVPRLQTSY*QAMAKIMK 393
IERY RAKF T +Y L P + QAM KIMK
Sbjct: 1723 IERYTRAKFMDYTTDNMSIYPSPTGVMIGLDLAYNLHSAFGNWFPGSKPLLAQAMNKIMK 1782
Query: 394 ANPALYVLRERIRKALQLYS*NLLNLTFPVRTMENCSXIKSFGSLM----TRMCIV*QLQ 561
+NPALYVLRERIRK LQLYS T P + +N I S + T + V +
Sbjct: 1783 SNPALYVLRERIRKGLQLYS---SEPTEPYLSSQNYGEIFSNQIIWFVDDTNVYRVTIHK 1839
Query: 562 NL*RKLDN*THQRSHFLYFNPRTGQLFLKIIHT 660
L + F+ FNPRTGQLFLK+IHT
Sbjct: 1840 TFEGNLTTKPINGAIFI-FNPRTGQLFLKVIHT 1871
Score = 85.0 bits (201), Expect = 4e-17
Identities = 56/129 (43%), Positives = 69/129 (53%), Gaps = 4/129 (3%)
Frame = +3
Query: 243 LDYTTDNMSIYLHLPVC*SPLIW-PITCTVHTVTGS--PVAN-LLLTSDGQDNES*SCLV 410
+DYTTDNMSIY P +I + +H+ G+ P + LL + + +S L
Sbjct: 1732 MDYTTDNMSIY---PSPTGVMIGLDLAYNLHSAFGNWFPGSKPLLAQAMNKIMKSNPALY 1788
Query: 411 CASGTYT*STAIVFLEPTEPYLSSQNYGELFXNQIIWFVDDTNVYRVTITKPLKET*QLN 590
+ EPTEPYLSSQNYGE+F NQIIWFVDDTNVYRVTI K +
Sbjct: 1789 VLRERIRKGLQLYSSEPTEPYLSSQNYGEIFSNQIIWFVDDTNVYRVTIHKTFEGNLTTK 1848
Query: 591 PSTEPFFIF 617
P FIF
Sbjct: 1849 PINGAIFIF 1857
Score = 76.6 bits (180), Expect = 1e-14
Identities = 47/113 (41%), Positives = 65/113 (57%), Gaps = 2/113 (1%)
Frame = +2
Query: 278 SPTGLLIAIDLAYNLHSAYGNWFPGCKPLINKRWPR**KLILPCMCFGNVYVKHCNCILR 457
SPTG++I +DLAYNLHSA+GNWFPG KPL+ + + K P + ++ +
Sbjct: 1744 SPTGVMIGLDLAYNLHSAFGNWFPGSKPLLAQAMNKIMK-SNPALYVLRERIRKGLQLYS 1802
Query: 458 TY*TLPFQSELWRIVL*SNHLVR**HE--CVSCNNYKTFEGNLTTKPINGAIF 610
+ T P+ S + SN ++ + +KTFEGNLTTKPINGAIF
Sbjct: 1803 SEPTEPYLSSQNYGEIFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIF 1855
>At4g38780.1 68417.m05491 splicing factor, putative strong similarity
to splicing factor Prp8 [Homo sapiens] GI:3661610;
contains Pfam profile PF01398: Mov34/MPN/PAD-1 family
Length = 2332
Score = 162 bits (394), Expect = 2e-40
Identities = 100/213 (46%), Positives = 122/213 (57%), Gaps = 5/213 (2%)
Frame = +1
Query: 37 PRKSYKMNSSCADILLFSAYKWNVSRPSLLADTKDTMDNTTTQKYWLDIQVGWGDYDSHD 216
PRKSYKMNSSCAD+LLF+A+KW +S+PSL+A++KD D + KYW+D+Q+ WGDYDSHD
Sbjct: 1615 PRKSYKMNSSCADVLLFAAHKWPMSKPSLIAESKDVFDQKASNKYWIDVQLRWGDYDSHD 1674
Query: 217 IERYARAKFW-ITLQTTCLYIFTYRFADRH*FGL*LAQCIR*LVPRLQTSY*QAMAKIMK 393
IERY +AKF T +Y L P + QAM KIMK
Sbjct: 1675 IERYTKAKFMDYTTDNMSIYPSPTGVIIGLDLAYNLHSAFGNWFPGSKPLLAQAMNKIMK 1734
Query: 394 ANPALYVLRERIRKALQLYS*NLLNLTFPVRTMENCSXIKSFGSLM----TRMCIV*QLQ 561
+NPALYVLRERIRK LQLYS T P + +N I S + T + V +
Sbjct: 1735 SNPALYVLRERIRKGLQLYS---SEPTEPYLSSQNYGEIFSNQIIWFVDDTNVYRVTIHK 1791
Query: 562 NL*RKLDN*THQRSHFLYFNPRTGQLFLKIIHT 660
L F+ FNPRTGQLFLKIIHT
Sbjct: 1792 TFEGNLTTKPINGVIFI-FNPRTGQLFLKIIHT 1823
Score = 84.6 bits (200), Expect = 5e-17
Identities = 56/129 (43%), Positives = 69/129 (53%), Gaps = 4/129 (3%)
Frame = +3
Query: 243 LDYTTDNMSIYLHLPVC*SPLIW-PITCTVHTVTGS--PVAN-LLLTSDGQDNES*SCLV 410
+DYTTDNMSIY P +I + +H+ G+ P + LL + + +S L
Sbjct: 1684 MDYTTDNMSIY---PSPTGVIIGLDLAYNLHSAFGNWFPGSKPLLAQAMNKIMKSNPALY 1740
Query: 411 CASGTYT*STAIVFLEPTEPYLSSQNYGELFXNQIIWFVDDTNVYRVTITKPLKET*QLN 590
+ EPTEPYLSSQNYGE+F NQIIWFVDDTNVYRVTI K +
Sbjct: 1741 VLRERIRKGLQLYSSEPTEPYLSSQNYGEIFSNQIIWFVDDTNVYRVTIHKTFEGNLTTK 1800
Query: 591 PSTEPFFIF 617
P FIF
Sbjct: 1801 PINGVIFIF 1809
Score = 74.9 bits (176), Expect = 4e-14
Identities = 46/113 (40%), Positives = 64/113 (56%), Gaps = 2/113 (1%)
Frame = +2
Query: 278 SPTGLLIAIDLAYNLHSAYGNWFPGCKPLINKRWPR**KLILPCMCFGNVYVKHCNCILR 457
SPTG++I +DLAYNLHSA+GNWFPG KPL+ + + K P + ++ +
Sbjct: 1696 SPTGVIIGLDLAYNLHSAFGNWFPGSKPLLAQAMNKIMK-SNPALYVLRERIRKGLQLYS 1754
Query: 458 TY*TLPFQSELWRIVL*SNHLVR**HE--CVSCNNYKTFEGNLTTKPINGAIF 610
+ T P+ S + SN ++ + +KTFEGNLTTKPING IF
Sbjct: 1755 SEPTEPYLSSQNYGEIFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGVIF 1807
>At5g59520.1 68418.m07459 zinc transporter (ZIP2) identical to zinc
transporter ZIP2 [Arabidopsis thaliana]
gi|3252868|gb|AAC24198; member of the Zinc (Zn2+)-Iron
(Fe2+) permease (ZIP) family, PMID:11500563
Length = 353
Score = 29.5 bits (63), Expect = 2.1
Identities = 18/69 (26%), Positives = 30/69 (43%)
Frame = +2
Query: 137 RTQWTIQLHKSTGLIYRLVGVITILTTSKDMLVLNFGLHYRQHVYISSPTGLLIAIDLAY 316
R WTI LHK + + ++ ++ L + + + ISSP G+ I I +
Sbjct: 231 RNLWTISLHKVFAAVAMGIALLKLIPKRPFFLTVVYSFAFG----ISSPIGVGIGIGINA 286
Query: 317 NLHSAYGNW 343
A G+W
Sbjct: 287 TSQGAGGDW 295
>At5g10520.1 68418.m01218 protein kinase family protein contains
protein kinase domain, INTERPRO:IPR000719
Length = 467
Score = 28.3 bits (60), Expect = 4.8
Identities = 23/73 (31%), Positives = 39/73 (53%)
Frame = +3
Query: 339 TGSPVANLLLTSDGQDNES*SCLVCASGTYT*STAIVFLEPTEPYLSSQNYGELFXNQII 518
+ SP L + SD DN S SC C+S + ST+ F T+ +SS ++G L N++I
Sbjct: 29 SSSPRGVLGMVSDS-DNSSSSCSSCSSDDKSSSTSSPFSNTTKT-VSSSHHG-LQWNKMI 85
Query: 519 WFVDDTNVYRVTI 557
+ ++ R ++
Sbjct: 86 ESIKKKSMRRFSV 98
>At5g15170.1 68418.m01777 tyrosyl-DNA phosphodiesterase-related
similar to Chain A, Crystal Structure Of Human
Tyrosyl-Dna Phosphodiesterase (Tdp1) [Homo sapiens]
GI:20150581
Length = 605
Score = 27.9 bits (59), Expect = 6.3
Identities = 10/40 (25%), Positives = 22/40 (55%)
Frame = +2
Query: 221 KDMLVLNFGLHYRQHVYISSPTGLLIAIDLAYNLHSAYGN 340
K L ++FG H+ + +++ P G+ + + A +H + N
Sbjct: 225 KPRLPISFGTHHSKAIFLVYPRGVRVVVHTANLIHVDWNN 264
>At5g45380.1 68418.m05577 sodium:solute symporter family protein
contains Pfam profile: PF00474 sodium:solute symporter
family
Length = 694
Score = 27.5 bits (58), Expect = 8.4
Identities = 10/38 (26%), Positives = 19/38 (50%)
Frame = +1
Query: 169 YWLDIQVGWGDYDSHDIERYARAKFWITLQTTCLYIFT 282
+W + + WG S I + W T+++ C+ +FT
Sbjct: 614 FWAIVAIAWGTIGSIVIIGLPLVESWDTIKSVCMGMFT 651
>At5g01620.2 68418.m00078 expressed protein several hypothetical
proteins - Arabidopsis thaliana
Length = 449
Score = 27.5 bits (58), Expect = 8.4
Identities = 14/68 (20%), Positives = 34/68 (50%), Gaps = 3/68 (4%)
Frame = +1
Query: 70 ADILLFSAYKW---NVSRPSLLADTKDTMDNTTTQKYWLDIQVGWGDYDSHDIERYARAK 240
ADIL+F+ Y W + + ++ K + + + + WGD+ +++++ +
Sbjct: 270 ADILIFNTYLWWRQDSVKLRWSSEEKGSCEEVKSAEGMEMAMDSWGDWVANNVDPNKKRV 329
Query: 241 FWITLQTT 264
F++T+ T
Sbjct: 330 FFVTMSPT 337
>At5g01620.1 68418.m00077 expressed protein several hypothetical
proteins - Arabidopsis thaliana
Length = 449
Score = 27.5 bits (58), Expect = 8.4
Identities = 14/68 (20%), Positives = 34/68 (50%), Gaps = 3/68 (4%)
Frame = +1
Query: 70 ADILLFSAYKW---NVSRPSLLADTKDTMDNTTTQKYWLDIQVGWGDYDSHDIERYARAK 240
ADIL+F+ Y W + + ++ K + + + + WGD+ +++++ +
Sbjct: 270 ADILIFNTYLWWRQDSVKLRWSSEEKGSCEEVKSAEGMEMAMDSWGDWVANNVDPNKKRV 329
Query: 241 FWITLQTT 264
F++T+ T
Sbjct: 330 FFVTMSPT 337
>At1g21640.1 68414.m02709 ATP-NAD kinase family protein contains
similarity to NAD kinase [Homo sapiens]
gi|20070086|gb|AAM01195; contains Pfam domain, PF01513:
ATP-NAD kinase
Length = 985
Score = 27.5 bits (58), Expect = 8.4
Identities = 15/54 (27%), Positives = 28/54 (51%)
Frame = -2
Query: 510 DXRTILHSSDWKGKVQ*VLRIQLQCFTYTFPKHIQGRISFHYLGHRLLIRGLQP 349
D + ++H ++ V LR++L+C Y K + G++ F L ++ RG P
Sbjct: 791 DLKRVIHGNNTLDGVYITLRMRLRCEIYRKGKAMPGKV-FDVLNEIVVDRGSNP 843
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,449,581
Number of Sequences: 28952
Number of extensions: 335331
Number of successful extensions: 769
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 739
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 767
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1383534864
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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