Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ceN-0644
         (571 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC776.15c |||dihydrolipoamide S-succinyltransferase, e2 compon...   133   2e-32
SPCC794.07 |||dihydrolipoamide S-acetyltransferase E2 |Schizosac...    59   5e-10
SPBC21C3.02c |sds3||Sds3-like family protein|Schizosaccharomyces...    31   0.090
SPAC18G6.10 |||chromosome segregation protein |Schizosaccharomyc...    28   1.1  
SPBC3H7.12 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    28   1.1  
SPCC550.15c |||ribosome biogenesis protein |Schizosaccharomyces ...    26   3.4  
SPBC1271.04c |||deoxyhypusine synthase|Schizosaccharomyces pombe...    26   3.4  
SPBC14C8.01c |cut2|SPBC1815.02c|securin|Schizosaccharomyces pomb...    26   4.5  
SPAC29A4.07 |srb6|med22|mediator complex subunit Srb6 |Schizosac...    25   5.9  

>SPBC776.15c |||dihydrolipoamide S-succinyltransferase, e2 component
           of oxoglutarate dehydrogenase complex
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 452

 Score =  133 bits (321), Expect = 2e-32
 Identities = 66/129 (51%), Positives = 89/129 (68%), Gaps = 4/129 (3%)
 Frame = +2

Query: 134 TRTEQRVKMNRGRQRIAQRLKDAQNTNAMLTTFNEIDMSHIMAFRKKHLDTFTKKHSIKL 313
           +R E RVKMNR R RIA+RLK++QN  A LTTFNE DMS ++A RKK+ D   K+  +K+
Sbjct: 217 SRNEDRVKMNRMRLRIAERLKESQNRAASLTTFNECDMSAVVALRKKYKDEILKETGVKI 276

Query: 314 GLMSPFVKAAANALMDQPVVNAVIE----ENEIIYRDYVDISVAVATPKGLVVPVIRNVQ 481
           G MS F KA   A+   P +N  IE     + ++YRD+ D+S+AVATPKGLV PVIRN +
Sbjct: 277 GFMSFFSKACTQAMKQIPAINGSIEGEGKGDTLVYRDFCDLSIAVATPKGLVTPVIRNAE 336

Query: 482 NMTYADIRA 508
           +M+  +I +
Sbjct: 337 SMSLLEIES 345



 Score = 27.5 bits (58), Expect = 1.5
 Identities = 14/25 (56%), Positives = 15/25 (60%)
 Frame = +3

Query: 492 MLIFELTIAGLAEKARTGKLTIEKM 566
           +L  E  IA L  KAR GKL IE M
Sbjct: 340 LLEIESAIATLGSKARAGKLAIEDM 364


>SPCC794.07 |||dihydrolipoamide S-acetyltransferase E2
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 483

 Score = 58.8 bits (136), Expect = 5e-10
 Identities = 44/141 (31%), Positives = 65/141 (46%)
 Frame = +2

Query: 80  ETATVKVPPQDYSKEIAGTRTEQRVKMNRGRQRIAQRLKDAQNTNAMLTTFNEIDMSHIM 259
           E A     P   + + A     + + ++  R+ IA RL +++N N        ++M  I+
Sbjct: 234 EAAAKATTPAASAADAAAPGDYEDLPLSNMRKIIASRLAESKNMNPHYYVTVSVNMEKII 293

Query: 260 AFRKKHLDTFTKKHSIKLGLMSPFVKAAANALMDQPVVNAVIEENEIIYRDYVDISVAVA 439
             R   L+        KL +    +KA   AL   P VNA    + I     VDIS+AVA
Sbjct: 294 RLRAA-LNAMADGR-YKLSVNDLVIKATTAALRQVPEVNAAWMGDFIRQYKNVDISMAVA 351

Query: 440 TPKGLVVPVIRNVQNMTYADI 502
           TP GL+ PVIRN   +  A+I
Sbjct: 352 TPSGLITPVIRNTHALGLAEI 372


>SPBC21C3.02c |sds3||Sds3-like family protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 491

 Score = 31.5 bits (68), Expect = 0.090
 Identities = 25/69 (36%), Positives = 31/69 (44%), Gaps = 9/69 (13%)
 Frame = +1

Query: 103 STGLQQRDRRHAHRATGQDEQG--EATHRSAFEGRSEHERHVDD---IQR----DRHVPH 255
           S  L++ D     +AT  D  G  E  H S  E  SEHE   D+   I+R    D  +P 
Sbjct: 171 SPSLKESDFESEEKATN-DNNGLIETNHNSKLEESSEHEEEEDEESNIERTEDSDHQIPQ 229

Query: 256 HGIP*EAPR 282
            G   EAPR
Sbjct: 230 RGGTLEAPR 238


>SPAC18G6.10 |||chromosome segregation protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 688

 Score = 27.9 bits (59), Expect = 1.1
 Identities = 20/104 (19%), Positives = 46/104 (44%), Gaps = 1/104 (0%)
 Frame = +2

Query: 155 KMNRGRQRIAQRLKDAQNTNAMLTTFNEIDMSHIMAFRKKHLDTFTKKHSIKLGLMSP-F 331
           K++ G Q +   L+  +N+N    + +E +++    +      +  K H+    L+SP F
Sbjct: 50  KLSSGPQHLLSHLQKEENSNTSKASSSEDEIAPKYLYPSSPSKSTKKPHNETEPLLSPQF 109

Query: 332 VKAAANALMDQPVVNAVIEENEIIYRDYVDISVAVATPKGLVVP 463
           +   +N      + +  + +N   ++ Y ++S  V T   +  P
Sbjct: 110 IDKPSNIETPVKIESPHVSQNN-TFQSYSELSPNVETSLTMKTP 152


>SPBC3H7.12 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 287

 Score = 27.9 bits (59), Expect = 1.1
 Identities = 14/55 (25%), Positives = 28/55 (50%)
 Frame = +2

Query: 278 LDTFTKKHSIKLGLMSPFVKAAANALMDQPVVNAVIEENEIIYRDYVDISVAVAT 442
           L++F K +S+ + ++ PFV    +A    P++ AV  +   + +   D+S    T
Sbjct: 225 LESFAK-NSVDIEMLKPFVSQRIDAFSQDPILLAVTAKLSALQKKVNDVSYRFKT 278


>SPCC550.15c |||ribosome biogenesis protein |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 463

 Score = 26.2 bits (55), Expect = 3.4
 Identities = 31/115 (26%), Positives = 52/115 (45%), Gaps = 4/115 (3%)
 Frame = +2

Query: 65  HAQAIETATVKVPPQDYSKEIAGTRTEQRVKMNRGRQRIAQRLKDAQNTNAMLTTFNEID 244
           H   ++     +PP   S E+   +     K N   Q+ A+  ++ +  N     ++E  
Sbjct: 29  HHYNLKRKVASLPP--LSAEVFAGKILSIQKQNEEVQKKAEFYQNCEVCNKKF--YSEGA 84

Query: 245 MSHIMAFRKKHLDTFTK--KHSIKLGLMSPFVKAAANAL--MDQPVVNAVIEENE 397
            S  MA  KKH D  +K  ++S    L S    + A++   M +PVV++ IEE E
Sbjct: 85  YSSHMA-SKKHRDNLSKFQRNSRIKKLQSEDASSIASSTLSMGEPVVDSEIEEEE 138


>SPBC1271.04c |||deoxyhypusine synthase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 350

 Score = 26.2 bits (55), Expect = 3.4
 Identities = 10/27 (37%), Positives = 19/27 (70%)
 Frame = +2

Query: 437 ATPKGLVVPVIRNVQNMTYADIRAHHS 517
           ATP+ LV+ ++ +++NM    +RA+ S
Sbjct: 243 ATPRPLVLDIVADIRNMNTHAVRANKS 269


>SPBC14C8.01c |cut2|SPBC1815.02c|securin|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 301

 Score = 25.8 bits (54), Expect = 4.5
 Identities = 8/10 (80%), Positives = 10/10 (100%)
 Frame = -1

Query: 175 LPPPVHLDPL 146
           +PPPVHLDP+
Sbjct: 131 MPPPVHLDPI 140


>SPAC29A4.07 |srb6|med22|mediator complex subunit Srb6
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 136

 Score = 25.4 bits (53), Expect = 5.9
 Identities = 12/50 (24%), Positives = 27/50 (54%)
 Frame = +2

Query: 179 IAQRLKDAQNTNAMLTTFNEIDMSHIMAFRKKHLDTFTKKHSIKLGLMSP 328
           +++++K    TN++ T+F  +D S     + K   T  + H +++ L+ P
Sbjct: 73  VSRQIKSYWLTNSLSTSFPTVDYSEPDLEKVKRTLTKLQNHLLEVSLIEP 122


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,177,983
Number of Sequences: 5004
Number of extensions: 41186
Number of successful extensions: 177
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 138
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 176
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 242064240
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)