Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-0641
(696 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g05710.1 68418.m00628 pleckstrin homology (PH) domain-contain... 38 0.006
At4g24580.1 68417.m03522 pleckstrin homology (PH) domain-contain... 33 0.24
At3g10180.1 68416.m01219 kinesin motor protein-related similar t... 30 1.3
At1g77730.1 68414.m09050 pleckstrin homology (PH) domain-contain... 30 1.3
At1g59610.1 68414.m06704 dynamin-like protein, putative (ADL3) s... 30 1.3
At1g10290.1 68414.m01159 dynamin-like protein 6 (ADL6) identical... 29 2.2
At5g57370.1 68418.m07168 expressed protein low similarity to nuc... 28 5.1
At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-contain... 28 5.1
At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-contain... 28 5.1
At5g12150.1 68418.m01426 pleckstrin homology (PH) domain-contain... 28 5.1
At3g56410.2 68416.m06274 expressed protein 27 9.0
At3g56410.1 68416.m06273 expressed protein 27 9.0
At2g31020.1 68415.m03782 oxysterol-binding family protein simila... 27 9.0
At1g06660.1 68414.m00706 expressed protein 27 9.0
>At5g05710.1 68418.m00628 pleckstrin homology (PH) domain-containing
protein similar to AtPH1 [Arabidopsis thaliana]
GI:5926716; contains Pfam profile PF00169: PH domain
Length = 144
Score = 37.9 bits (84), Expect = 0.006
Identities = 37/132 (28%), Positives = 57/132 (43%), Gaps = 2/132 (1%)
Frame = +3
Query: 84 GWLTKSPPTKRIFRTKWQKRWFALRQSGELPGQYFLDYYSDRNCRRHKGTINLDLCEQVD 263
GWLTK + W++RWF L+Q G+L +F D R R +G + ++ C
Sbjct: 32 GWLTKQGE----YIKTWRRRWFVLKQ-GKL--FWFKDSDVTR-VSRPRGVVPVESC---- 79
Query: 264 AGLHMERSGNGTLDPKIRGSVFTIQTQTRTYHLEAECEAEMEKWVDVICR--VCGLRSTD 437
+ G D + + F + T+ T + A+ E E E W++ I R V RS
Sbjct: 80 ------LTAKGAEDVLNKQNAFELSTRNETMYFIADSEKEKEDWINSIGRSIVQNSRSVT 133
Query: 438 DDRPEAVASYQN 473
D + Y N
Sbjct: 134 DSE---IVDYDN 142
>At4g24580.1 68417.m03522 pleckstrin homology (PH) domain-containing
protein-related / RhoGAP domain-containing protein
contains Pfam domain, PF00620: RhoGAP domain
Length = 902
Score = 32.7 bits (71), Expect = 0.24
Identities = 26/99 (26%), Positives = 48/99 (48%), Gaps = 3/99 (3%)
Frame = +3
Query: 108 TKRIFRTKWQKRWFALRQSGELPGQYFLDYYSDRNCRRHKGT-INLDLCEQVDAGLHMER 284
+K I T W+KRWF L ++ + + SD + + KG+ +NL L G+ +
Sbjct: 71 SKGIGWTSWKKRWFILTRTS------LVFFRSDPSAVQQKGSEVNLTL-----GGIDLNN 119
Query: 285 SGNGTL--DPKIRGSVFTIQTQTRTYHLEAECEAEMEKW 395
SG+ + D K+ +F R + L+A+ ++ +W
Sbjct: 120 SGSVVVKADKKLLTVLFPDGRDGRAFTLKADTMEDLHEW 158
>At3g10180.1 68416.m01219 kinesin motor protein-related similar to
centromere protein E GB:4502781 [Homo sapiens]
Length = 1348
Score = 30.3 bits (65), Expect = 1.3
Identities = 22/77 (28%), Positives = 32/77 (41%), Gaps = 1/77 (1%)
Frame = +3
Query: 270 LHMERSGNGTLDPKIRG-SVFTIQTQTRTYHLEAECEAEMEKWVDVICRVCGLRSTDDDR 446
L +ERS N +L + + V + +T LE E AE + + +CR GL S D
Sbjct: 889 LLVERSANQSLQEEFKQLEVLAFEMETTIASLEEELAAERGEKEEALCRNDGLGSEITDL 948
Query: 447 PEAVASYQNNTRAASND 497
E + ND
Sbjct: 949 TEKLEHSNTKLEHLQND 965
>At1g77730.1 68414.m09050 pleckstrin homology (PH) domain-containing
protein similar to SP|P16258 Oxysterol-binding protein 1
{Oryctolagus cuniculus}; contains Pfam profile PF00169:
PH domain
Length = 265
Score = 30.3 bits (65), Expect = 1.3
Identities = 27/108 (25%), Positives = 42/108 (38%), Gaps = 16/108 (14%)
Frame = +3
Query: 132 WQKRWFALRQSGEL-------PGQYFLDYYSDRNCRRHKGTINLDLCEQVDAG-LHMERS 287
W++RWF L Q G L P + L DR + G +C G +H
Sbjct: 68 WKRRWFVL-QDGVLSYYRIHGPDKISLSVEMDRRSKLIGGESLRFICRHSKRGDVHSPGK 126
Query: 288 GNGTLDPKIR--------GSVFTIQTQTRTYHLEAECEAEMEKWVDVI 407
G + K+ G FT+ T T++ HL A + W++ +
Sbjct: 127 PLGQIHLKVSSIGQSISDGKRFTVFTGTKSLHLRAATSEDRASWIEAL 174
>At1g59610.1 68414.m06704 dynamin-like protein, putative (ADL3)
strong similarity to dynamin-like protein 6 (ADL6)
[Arabidopsis thaliana] GI:6651399; contains Pfam
profiles PF01031: Dynamin central region, PF00350:
Dynamin family, PF02212: Dynamin GTPase effector domain,
PF00169: PH domain; identical to cDNA dynamin-like
protein ADL3, GI:4803835
Length = 920
Score = 30.3 bits (65), Expect = 1.3
Identities = 37/134 (27%), Positives = 61/134 (45%), Gaps = 18/134 (13%)
Frame = +3
Query: 81 EGWLTKSPPTKRIFRTK-WQKRWFALRQSGELPGQYFLDYYSDRNCRRHKGTINLDLC-- 251
EG +T K+ +T W +RWF L E G+ L Y + R +GT+ L+ C
Sbjct: 578 EGEITAGYLMKKSAKTNGWSRRWFVL---NEKTGK--LGYTKKQEERNFRGTVTLEECSI 632
Query: 252 EQV--DAG----LHMERSGNGTLDPKIRGSVFTI------QTQTRTYH---LEAECEAEM 386
E++ D G ++ NG D K G VF I +T + ++ L+AE +
Sbjct: 633 EEISDDEGEKSKSSKDKKSNGP-DSKGPGLVFKITCRVPYKTVLKAHNALVLKAESMVDK 691
Query: 387 EKWVDVICRVCGLR 428
+W++ + +V R
Sbjct: 692 NEWINKLQKVIQAR 705
>At1g10290.1 68414.m01159 dynamin-like protein 6 (ADL6) identical to
dynamin-like protein 6 (ADL6) [Arabidopsis thaliana]
GI:6651399; contains Pfam profiles PF01031: Dynamin
central region, PF00350: Dynamin family, PF02212:
Dynamin GTPase effector domain, PF00169: PH domain
Length = 914
Score = 29.5 bits (63), Expect = 2.2
Identities = 20/58 (34%), Positives = 28/58 (48%), Gaps = 1/58 (1%)
Frame = +3
Query: 81 EGWLTKSPPTKRIFRTK-WQKRWFALRQSGELPGQYFLDYYSDRNCRRHKGTINLDLC 251
EG +T K+ +T W +RWF L E G+ L Y + R +GTI L+ C
Sbjct: 571 EGEITAGYLMKKSAKTNGWSRRWFVL---NEKTGK--LGYTKKQEERNFRGTITLEEC 623
>At5g57370.1 68418.m07168 expressed protein low similarity to
nucleic acid binding protein [Homo sapiens] GI:431953
Length = 219
Score = 28.3 bits (60), Expect = 5.1
Identities = 18/56 (32%), Positives = 24/56 (42%), Gaps = 2/56 (3%)
Frame = +3
Query: 426 RSTDDDRPEAVASYQNNTRAASNDHYHRASETTGP--YIPISECITGVRDQDDQRH 587
R D +R +R+ + DH+ RA P Y S I RD+D QRH
Sbjct: 36 RERDRERDRDRGLRNKKSRSRTPDHHARARHVRSPERYRSRSRSIDRDRDRDRQRH 91
>At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-containing
protein / RhoGAP domain-containing protein weak
similarity to rho-GTPase activating protein [Homo
sapiens] GI:14245732; contains Pfam profiles PF00169: PH
domain, PF00620: RhoGAP domain
Length = 870
Score = 28.3 bits (60), Expect = 5.1
Identities = 24/93 (25%), Positives = 42/93 (45%), Gaps = 3/93 (3%)
Frame = +3
Query: 126 TKWQKRWFALRQSGELPGQYFLDYYSDRNCRRHK-GTINLDLCEQVDAGLHMERSGNGTL 302
T W+KRWF L ++ + + +D K G +NL L G+ + SG+ +
Sbjct: 35 TSWKKRWFILTRTS------LVFFKNDPGTLPQKGGEVNLTL-----GGIDLNNSGSVVV 83
Query: 303 --DPKIRGSVFTIQTQTRTYHLEAECEAEMEKW 395
D K+ +F R + L+AE ++ +W
Sbjct: 84 REDKKLLTVLFPDGRDGRAFTLKAETFEDLYEW 116
>At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-containing
protein / RhoGAP domain-containing protein weak
similarity to rho-GTPase activating protein [Homo
sapiens] GI:14245732; contains Pfam profiles PF00169: PH
domain, PF00620: RhoGAP domain
Length = 822
Score = 28.3 bits (60), Expect = 5.1
Identities = 24/93 (25%), Positives = 42/93 (45%), Gaps = 3/93 (3%)
Frame = +3
Query: 126 TKWQKRWFALRQSGELPGQYFLDYYSDRNCRRHK-GTINLDLCEQVDAGLHMERSGNGTL 302
T W+KRWF L ++ + + +D K G +NL L G+ + SG+ +
Sbjct: 35 TSWKKRWFILTRTS------LVFFKNDPGTLPQKGGEVNLTL-----GGIDLNNSGSVVV 83
Query: 303 --DPKIRGSVFTIQTQTRTYHLEAECEAEMEKW 395
D K+ +F R + L+AE ++ +W
Sbjct: 84 REDKKLLTVLFPDGRDGRAFTLKAETFEDLYEW 116
>At5g12150.1 68418.m01426 pleckstrin homology (PH) domain-containing
protein / RhoGAP domain-containing protein weak
similarity to glucocorticoid receptor DNA binding factor
1 [Canis familiaris] GI:23266717; contains Pfam profiles
PF00169: PH domain, PF00620: RhoGAP domain
Length = 827
Score = 28.3 bits (60), Expect = 5.1
Identities = 24/93 (25%), Positives = 43/93 (46%), Gaps = 3/93 (3%)
Frame = +3
Query: 126 TKWQKRWFALRQSGELPGQYFLDYYSDRNCRRHK-GTINLDLCEQVDAGLHMERSGNGTL 302
T W+KRWF L ++ + + +D + K G +NL L G+ + SG+ +
Sbjct: 35 TSWKKRWFILTRTS------LVFFKNDPSALPQKGGEVNLTL-----GGIDLNSSGSVVV 83
Query: 303 --DPKIRGSVFTIQTQTRTYHLEAECEAEMEKW 395
D K+ +F R + L+AE ++ +W
Sbjct: 84 REDKKLLTVLFPDGRDGRAFTLKAETLDDLYEW 116
>At3g56410.2 68416.m06274 expressed protein
Length = 1535
Score = 27.5 bits (58), Expect = 9.0
Identities = 17/62 (27%), Positives = 29/62 (46%)
Frame = +3
Query: 273 HMERSGNGTLDPKIRGSVFTIQTQTRTYHLEAECEAEMEKWVDVICRVCGLRSTDDDRPE 452
H++ GN L + S TI +T HLE EA EK ++ +C + + ++ E
Sbjct: 522 HLKPMGNRRLSEQQPTSSETIG-ETSKIHLEQSQEAHSEKSTEMDNNICDIEEPEYEKNE 580
Query: 453 AV 458
+
Sbjct: 581 II 582
>At3g56410.1 68416.m06273 expressed protein
Length = 1488
Score = 27.5 bits (58), Expect = 9.0
Identities = 17/62 (27%), Positives = 29/62 (46%)
Frame = +3
Query: 273 HMERSGNGTLDPKIRGSVFTIQTQTRTYHLEAECEAEMEKWVDVICRVCGLRSTDDDRPE 452
H++ GN L + S TI +T HLE EA EK ++ +C + + ++ E
Sbjct: 475 HLKPMGNRRLSEQQPTSSETIG-ETSKIHLEQSQEAHSEKSTEMDNNICDIEEPEYEKNE 533
Query: 453 AV 458
+
Sbjct: 534 II 535
>At2g31020.1 68415.m03782 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 760
Score = 27.5 bits (58), Expect = 9.0
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +3
Query: 327 FTIQTQTRTYHLEAECEAEMEKWVDVI 407
F+I T T+ HL AE + E W++ +
Sbjct: 172 FSIFTGTKRLHLRAETREDREAWIEAL 198
>At1g06660.1 68414.m00706 expressed protein
Length = 481
Score = 27.5 bits (58), Expect = 9.0
Identities = 8/26 (30%), Positives = 17/26 (65%)
Frame = +1
Query: 577 TKGTVPVLGIIGLPWAGSRRTKLTPK 654
T G P++G++ W + +T+++PK
Sbjct: 379 TPGDRPIIGLVAAQWIENEQTEISPK 404
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,125,138
Number of Sequences: 28952
Number of extensions: 366328
Number of successful extensions: 957
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 927
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 954
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1487069504
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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