BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ceN-0630 (522 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g13950.1 68414.m01639 eukaryotic translation initiation facto... 193 5e-50 At1g69410.1 68414.m07972 eukaryotic translation initiation facto... 188 3e-48 At1g26630.1 68414.m03243 eukaryotic translation initiation facto... 182 1e-46 At3g26570.2 68416.m03317 phosphate transporter family protein co... 33 0.15 At3g26570.1 68416.m03316 phosphate transporter family protein co... 33 0.15 At3g58930.1 68416.m06567 F-box family protein contains F-box dom... 30 0.82 At5g14230.1 68418.m01663 ankyrin repeat family protein contains ... 30 1.1 At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative simila... 29 1.4 At1g56345.1 68414.m06477 pseudouridine synthase family protein l... 29 1.4 At5g41460.1 68418.m05035 fringe-related protein strong similarit... 29 2.5 At5g03140.1 68418.m00262 lectin protein kinase family protein co... 29 2.5 At3g53330.1 68416.m05884 plastocyanin-like domain-containing pro... 28 4.4 At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein... 27 5.8 At5g64290.1 68418.m08076 oxoglutarate/malate translocator, putat... 27 7.7 At5g64280.1 68418.m08075 oxoglutarate/malate translocator, putat... 27 7.7 At3g51290.1 68416.m05614 proline-rich family protein 27 7.7 At3g12240.1 68416.m01527 serine carboxypeptidase S10 family prot... 27 7.7 At2g27680.1 68415.m03354 aldo/keto reductase family protein cont... 27 7.7 At1g09780.1 68414.m01097 2,3-biphosphoglycerate-independent phos... 27 7.7 >At1g13950.1 68414.m01639 eukaryotic translation initiation factor 5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Arabidopsis thaliana} Length = 158 Score = 193 bits (471), Expect = 5e-50 Identities = 84/150 (56%), Positives = 115/150 (76%), Gaps = 1/150 (0%) Frame = +3 Query: 66 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRGCKIVEMSTSKTGKHGHAKVHLVGID 245 E+ HFE+ D+GAS T+P Q +RKNG++++K R CK+VE+STSKTGKHGHAK H V ID Sbjct: 4 EEHHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63 Query: 246 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGD-LG 422 IF KK EDI PS+HN DVPHV R DYQL DIS+DGY++L+ DNG ++DLK+P+ D L Sbjct: 64 IFTSKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLL 123 Query: 423 TQLRTDFNSGKELLCTVLKSCGEECVIPVK 512 Q+++ F+ GK+L+ +V+ + GEE + +K Sbjct: 124 QQIKSGFDDGKDLVVSVMSAMGEEQINALK 153 >At1g69410.1 68414.m07972 eukaryotic translation initiation factor 5A, putative / eIF-5A, putative strong similarity to eukaryotic initiation factor 5A (2) (Nicotiana plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Lycopersicon esculentum} Length = 158 Score = 188 bits (457), Expect = 3e-48 Identities = 83/150 (55%), Positives = 112/150 (74%), Gaps = 1/150 (0%) Frame = +3 Query: 66 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRGCKIVEMSTSKTGKHGHAKVHLVGID 245 ++ HFE+ D+GAS T+P Q +RK G +++KGR CK+VE+STSKTGKHGHAK H V ID Sbjct: 4 DEHHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAID 63 Query: 246 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLG 422 IF KK EDI PS+HN DVPHV R DYQL DIS+DG+++L+ DNG ++DLK+P D L Sbjct: 64 IFTSKKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALL 123 Query: 423 TQLRTDFNSGKELLCTVLKSCGEECVIPVK 512 TQL+ F GK+++ +V+ + GEE + +K Sbjct: 124 TQLKNGFEEGKDIVVSVMSAMGEEQMCALK 153 >At1g26630.1 68414.m03243 eukaryotic translation initiation factor 5A, putative / eIF-5A, putative strong similariy to SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Lycopersicon esculentum} Length = 159 Score = 182 bits (443), Expect = 1e-46 Identities = 80/150 (53%), Positives = 109/150 (72%), Gaps = 1/150 (0%) Frame = +3 Query: 66 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRGCKIVEMSTSKTGKHGHAKVHLVGID 245 ++ HFE +SGAS T+P +RK G +++K R CK+VE+STSKTGKHGHAK H V ID Sbjct: 4 DEHHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63 Query: 246 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLG 422 IF KK EDI PS+HN DVPHV R DYQL DI++DG+++L+ D+G ++DLK+P D L Sbjct: 64 IFTAKKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLT 123 Query: 423 TQLRTDFNSGKELLCTVLKSCGEECVIPVK 512 Q+R F+ GK+++ +V+ S GEE + VK Sbjct: 124 AQMRLGFDEGKDIVVSVMSSMGEEQICAVK 153 >At3g26570.2 68416.m03317 phosphate transporter family protein contains Pfam profile: PF01384 phosphate transporter family Length = 587 Score = 32.7 bits (71), Expect = 0.15 Identities = 18/37 (48%), Positives = 22/37 (59%) Frame = -3 Query: 487 PQDFSTVHNNSLPLLKSVRNCVPRSPSGILRSSRRSP 377 P FS+V N+SL LLK+ C PRS G S + SP Sbjct: 4 PYRFSSVRNHSL-LLKTSHLCTPRSALGCCFSPKESP 39 >At3g26570.1 68416.m03316 phosphate transporter family protein contains Pfam profile: PF01384 phosphate transporter family Length = 613 Score = 32.7 bits (71), Expect = 0.15 Identities = 18/37 (48%), Positives = 22/37 (59%) Frame = -3 Query: 487 PQDFSTVHNNSLPLLKSVRNCVPRSPSGILRSSRRSP 377 P FS+V N+SL LLK+ C PRS G S + SP Sbjct: 30 PYRFSSVRNHSL-LLKTSHLCTPRSALGCCFSPKESP 65 >At3g58930.1 68416.m06567 F-box family protein contains F-box domain Pfam:PF00646 Length = 482 Score = 30.3 bits (65), Expect = 0.82 Identities = 27/88 (30%), Positives = 40/88 (45%), Gaps = 11/88 (12%) Frame = -3 Query: 286 VEGQISSYFLPLK----ISIPTK*TL-AWPCFPVLDVDISTILHPRP-----FSITKPFL 137 V G I S FLP K S+ +K L W P+LD+D S LHP I + F+ Sbjct: 11 VRGHILS-FLPAKHIALTSVLSKSWLNLWKLIPILDIDDSEFLHPEEGKAERLEIRQSFV 69 Query: 136 RRAEHCMGKVAEAPESPVS-KCVSSMSP 56 + + ++P S KC++ + P Sbjct: 70 DFVDRVLALQDDSPIDRFSLKCITGIHP 97 >At5g14230.1 68418.m01663 ankyrin repeat family protein contains ankyrin repeats, Pfam:PF00023 Length = 591 Score = 29.9 bits (64), Expect = 1.1 Identities = 15/34 (44%), Positives = 16/34 (47%) Frame = +3 Query: 396 LKIPDGDLGTQLRTDFNSGKELLCTVLKSCGEEC 497 L IPDGD T L G +C L SCG C Sbjct: 412 LDIPDGDGYTPLMLAAREGHGHMCEYLISCGANC 445 >At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative similar to SP|P35207 Antiviral protein SKI2 {Saccharomyces cerevisiae}; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 1347 Score = 29.5 bits (63), Expect = 1.4 Identities = 18/51 (35%), Positives = 30/51 (58%), Gaps = 6/51 (11%) Frame = +3 Query: 339 ISDDGYLTLMADNG--DLREDLKIPDGDLGTQLR----TDFNSGKELLCTV 473 +SD+ L + A G D+ +++ D DL Q++ + NSG+EL+CTV Sbjct: 1141 MSDEALLQMPAFQGRIDVLKNIGCIDDDLVVQIKGRVACEMNSGEELICTV 1191 >At1g56345.1 68414.m06477 pseudouridine synthase family protein low similarity to SP|P23851 Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate synthase) (Uracil hydrolyase) {Escherichia coli}; contains Pfam profile PF00849: RNA pseudouridylate synthase Length = 322 Score = 29.5 bits (63), Expect = 1.4 Identities = 20/69 (28%), Positives = 35/69 (50%), Gaps = 1/69 (1%) Frame = -2 Query: 476 QHGAQQFLAAVEVSTQLCAEVTIRDFEVLTQ-VTVVSHQGQVAIIRDISQLVVFALHVGY 300 +HGA + AA++V L +RD E + V+V S + + + D++ ++V G Sbjct: 176 KHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSVNSVKNESCELEDVNHVIVAE---GE 232 Query: 299 VHVVCGGTD 273 + CGG D Sbjct: 233 RELSCGGDD 241 >At5g41460.1 68418.m05035 fringe-related protein strong similarity to unknown protein (pir||T13026) similarity to predicted proteins + similar to hypothetical protein GB:AAC23643 [Arabidopsis thaliana] + weak similarity to Fringe [Schistocerca gregaria](GI:6573138);Fringe encodes an extracellular protein that regulates Notch signalling. Length = 524 Score = 28.7 bits (61), Expect = 2.5 Identities = 11/29 (37%), Positives = 16/29 (55%) Frame = +1 Query: 31 VVKFKTQQWVTSKTHTSRPETPGPQPPSP 117 ++ F T Q K++ S P +P P PP P Sbjct: 80 LILFHTNQTAVIKSYASPPPSPPPPPPPP 108 >At5g03140.1 68418.m00262 lectin protein kinase family protein contains Pfam domains, PF00138: Legume lectins alpha domain, PF00139: Legume lectins beta domain and PF00069: Protein kinase domain Length = 711 Score = 28.7 bits (61), Expect = 2.5 Identities = 13/36 (36%), Positives = 21/36 (58%) Frame = -3 Query: 166 RPFSITKPFLRRAEHCMGKVAEAPESPVSKCVSSMS 59 +P +T+P +R + A+ PE P++K SSMS Sbjct: 631 QPDPVTRPTMRSVVQILVGEADVPEVPIAKPSSSMS 666 >At3g53330.1 68416.m05884 plastocyanin-like domain-containing protein similar to mavicyanin SP:P80728 from [Cucurbita pepo] Length = 310 Score = 27.9 bits (59), Expect = 4.4 Identities = 13/18 (72%), Positives = 13/18 (72%), Gaps = 1/18 (5%) Frame = +1 Query: 64 SKTHT-SRPETPGPQPPS 114 SKTH SRP TP P PPS Sbjct: 127 SKTHERSRPITPSPPPPS 144 >At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein contains INTERPRO domain, IPR001878: Zn-finger CCHC type Length = 353 Score = 27.5 bits (58), Expect = 5.8 Identities = 15/45 (33%), Positives = 16/45 (35%), Gaps = 3/45 (6%) Frame = +1 Query: 67 KTHTSRPETPGPQPPS---PCNVRPCVKTVSLC*RVGGARLLKCP 192 KT T P PP PCN PC S + R CP Sbjct: 22 KTTTKPTAAAAPSPPDIHCPCNAGPCNTVTSKTEKNPNRRFYTCP 66 >At5g64290.1 68418.m08076 oxoglutarate/malate translocator, putative similar to SWISS-PROT:Q41364 2-oxoglutarate/malate translocator, chloroplast precursor. [Spinach]{Spinacia oleracea} Length = 563 Score = 27.1 bits (57), Expect = 7.7 Identities = 16/70 (22%), Positives = 26/70 (37%) Frame = +1 Query: 94 PGPQPPSPCNVRPCVKTVSLC*RVGGARLLKCPHPKPESTATLKFTWLGLISSMVKSMKI 273 P P PSP K + L + +L+ P PE + L + S + + + Sbjct: 80 PPPPSPSPSPSPQGAKLIPLILSISVGLILRFAVPVPEGVTPQGWQLLSIFLSTIAGLVL 139 Query: 274 SVPPHTTWTY 303 S P W + Sbjct: 140 SPLPVGAWAF 149 >At5g64280.1 68418.m08075 oxoglutarate/malate translocator, putative similar to SWISS-PROT:Q41364 2-oxoglutarate/malate translocator, chloroplast precursor [Spinach]{Spinacia oleracea} Length = 549 Score = 27.1 bits (57), Expect = 7.7 Identities = 15/75 (20%), Positives = 30/75 (40%) Frame = +1 Query: 79 SRPETPGPQPPSPCNVRPCVKTVSLC*RVGGARLLKCPHPKPESTATLKFTWLGLISSMV 258 ++P+ P P PP P K + L V +++ P+PE + + L + + Sbjct: 65 NKPQPPPPSPPQPQG----AKLIPLAISVSIGLIVRFLIPRPEQVTSQGWQLLSIFLFTI 120 Query: 259 KSMKISVPPHTTWTY 303 + + P W + Sbjct: 121 SGLVLGPLPVGAWAF 135 >At3g51290.1 68416.m05614 proline-rich family protein Length = 602 Score = 27.1 bits (57), Expect = 7.7 Identities = 10/25 (40%), Positives = 13/25 (52%) Frame = +1 Query: 43 KTQQWVTSKTHTSRPETPGPQPPSP 117 +T W T+ T + P P P PP P Sbjct: 91 ETTTWTTTTTSSVLPPPPPPPPPPP 115 >At3g12240.1 68416.m01527 serine carboxypeptidase S10 family protein contains Pfam profile: PF00450 serine carboxypeptidase; similar to serine carboxypeptidase I precursor (SP:P37890) [Oryza sativa] Length = 436 Score = 27.1 bits (57), Expect = 7.7 Identities = 20/74 (27%), Positives = 32/74 (43%), Gaps = 4/74 (5%) Frame = -2 Query: 383 VTVVSHQGQVAIIRDISQL---VVFALHVGYVHVVCGGTD-IFILFTIEDINPNQVNFSV 216 + ++ H +IIR + + F L GY+ V D +F F + NP + V Sbjct: 16 LVLIQHADSSSIIRYLPGFEGPLPFELETGYIGVGQKEEDQLFYYFIKSENNPEEDPLLV 75 Query: 215 AVLSGFGCGHFNNL 174 + G GC F+ L Sbjct: 76 WLTGGPGCSSFSGL 89 >At2g27680.1 68415.m03354 aldo/keto reductase family protein contains Pfam profile PF00248: oxidoreductase, aldo/keto reductase family Length = 384 Score = 27.1 bits (57), Expect = 7.7 Identities = 15/46 (32%), Positives = 24/46 (52%) Frame = +3 Query: 267 EDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKI 404 ++I S MDV + + D ++DGYL + DL+E+ KI Sbjct: 147 QNIDISRKRMDVAALDMLQFHWWDYANDGYLDALKHLTDLKEEGKI 192 >At1g09780.1 68414.m01097 2,3-biphosphoglycerate-independent phosphoglycerate mutase, putative / phosphoglyceromutase, putative strong similarity to SP|Q42908 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (EC 5.4.2.1) (Phosphoglyceromutase) {Mesembryanthemum crystallinum}; contains Pfam profile PF01676: Metalloenzyme superfamily Length = 557 Score = 27.1 bits (57), Expect = 7.7 Identities = 10/34 (29%), Positives = 18/34 (52%) Frame = -3 Query: 520 VFALTGMTHSSPQDFSTVHNNSLPLLKSVRNCVP 419 V L G S+P ++ +HN P + S+++ P Sbjct: 23 VIVLDGWGESAPDQYNCIHNAPTPAMDSLKHGAP 56 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,646,257 Number of Sequences: 28952 Number of extensions: 286506 Number of successful extensions: 957 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 872 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 949 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 957410176 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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