Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-0591
(684 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g48920.1 68414.m05480 nucleolin, putative similar to nuM1 pro... 33 0.13
At5g63550.1 68418.m07976 expressed protein 32 0.31
At5g26200.1 68418.m03118 mitochondrial substrate carrier family ... 32 0.41
At3g49990.1 68416.m05466 expressed protein 31 0.54
At1g22530.1 68414.m02814 SEC14 cytosolic factor family protein /... 30 1.2
At1g62480.1 68414.m07050 vacuolar calcium-binding protein-relate... 29 2.2
At3g10660.1 68416.m01282 calcium-dependent protein kinase isofor... 27 2.6
At2g45800.1 68415.m05696 LIM domain-containing protein similar t... 29 3.8
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 29 3.8
At1g03100.1 68414.m00285 pentatricopeptide (PPR) repeat-containi... 28 5.0
At2g02170.1 68415.m00153 remorin family protein contains Pfam do... 28 6.6
At4g25610.1 68417.m03689 zinc finger (C2H2 type) family protein ... 27 8.8
At4g20260.2 68417.m02960 DREPP plasma membrane polypeptide famil... 27 8.8
At4g20260.1 68417.m02959 DREPP plasma membrane polypeptide famil... 27 8.8
>At1g48920.1 68414.m05480 nucleolin, putative similar to nuM1
protein GI:1279562 from [Medicago sativa]
Length = 557
Score = 33.5 bits (73), Expect = 0.13
Identities = 15/51 (29%), Positives = 28/51 (54%)
Frame = +3
Query: 405 PDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSA 557
P A AK+ +K ++ ++ +DS +E + +K KP+A D+ S D +
Sbjct: 141 PAAAAKNGSVKAKKESSSEDDSSSEDEPAKKPAAKIAKPAAKDSSSSDDDS 191
Score = 27.5 bits (58), Expect = 8.8
Identities = 19/81 (23%), Positives = 35/81 (43%)
Frame = +3
Query: 297 AKKDDIAPEDSDIAXXXXXXXXXXXXXXXXAKSSEIPDAEAKSADIKVEEPAAQPEDSKT 476
+ DD + EDS+ + S D++ +S D E+PA + D+K
Sbjct: 185 SSSDDDSDEDSEDEKPATKKAAPAAAKAASSSDSSDEDSDEESED---EKPAQKKADTKA 241
Query: 477 EVQATVAEISKEEKPSATDAE 539
+++ E S+ E+ + D E
Sbjct: 242 SKKSSSDESSESEEDESEDEE 262
>At5g63550.1 68418.m07976 expressed protein
Length = 530
Score = 32.3 bits (70), Expect = 0.31
Identities = 19/72 (26%), Positives = 37/72 (51%)
Frame = +3
Query: 399 EIPDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSAAIIPNMV 578
E D + + +++VE+P+ + SK V+ + K+++PS A+GSA S +
Sbjct: 320 EKTDTDDEKDEVEVEKPSKKKSSSKKTVEESSGSKGKDKQPS---AKGSARSGEKSSKQI 376
Query: 579 KKIDLAPNVKKR 614
K +P K++
Sbjct: 377 AKSTSSPAKKQK 388
>At5g26200.1 68418.m03118 mitochondrial substrate carrier family
protein contains Pfam profile: PF00153 mitochondrial
carrier protein
Length = 342
Score = 31.9 bits (69), Expect = 0.41
Identities = 17/35 (48%), Positives = 24/35 (68%)
Frame = -3
Query: 211 LTESSILGATSRIG*AGTTSFSSATGIAKLTAAKA 107
+T+SS+ AT R+G + TTS + A G A LT+A A
Sbjct: 112 ITKSSVGQATVRLGLSDTTSLAVANGAAGLTSAVA 146
>At3g49990.1 68416.m05466 expressed protein
Length = 502
Score = 31.5 bits (68), Expect = 0.54
Identities = 17/46 (36%), Positives = 25/46 (54%)
Frame = +3
Query: 402 IPDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAE 539
+P A+ D+K E P A+P KT Q + E K+E+ +A AE
Sbjct: 422 LPGRRAEQTDVKAEIPKAEPIKRKTHGQESKEE--KKERKNAVKAE 465
>At1g22530.1 68414.m02814 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus; similar to SEC14-like protein 2
(Alpha-tocopherol associated protein) (TAP) (bTAP)
(Fragment) (SP:P58875) {Bos taurus}
Length = 683
Score = 30.3 bits (65), Expect = 1.2
Identities = 16/41 (39%), Positives = 23/41 (56%)
Frame = +3
Query: 405 PDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSA 527
P E K + K EE + E+ KTE ++ AE +EEK +A
Sbjct: 123 PVKEEKVEEKKTEETEEKKEEVKTEEKSLEAETKEEEKSAA 163
>At1g62480.1 68414.m07050 vacuolar calcium-binding protein-related
contains weak similarity to vacuolar calcium binding
protein [Raphanus sativus] gi|9049359|dbj|BAA99394
Length = 152
Score = 29.5 bits (63), Expect = 2.2
Identities = 21/83 (25%), Positives = 38/83 (45%), Gaps = 2/83 (2%)
Frame = +3
Query: 276 EVPAIPEAKKDDIAPEDSDIAXXXXXXXXXXXXXXXXAKSSEIPDAEAKSADIKV-EEPA 452
E P + E KKD+ AP ++ + K E+ + + + A + V EE
Sbjct: 73 ETPEVVEIKKDEEAPVETPVV---VEDESKTEEVVEAKKEEEVEEKKTEEAPVVVEEEKK 129
Query: 453 AQPEDSKTEVQATV-AEISKEEK 518
+ E+ K V+A+V A + K ++
Sbjct: 130 PEAEEEKPAVEASVTAPVEKADE 152
>At3g10660.1 68416.m01282 calcium-dependent protein kinase isoform 2
(CPK2) identical to calcium-dependent protein kinase
isoform 2 [Arabidopsis thaliana] gi|9837343|gb|AAG00535;
contains protein kinase domain, Pfam:PF00069; contains
EF hand domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 646
Score = 27.1 bits (57), Expect(2) = 2.6
Identities = 17/48 (35%), Positives = 29/48 (60%), Gaps = 1/48 (2%)
Frame = +3
Query: 399 EIPDAEAKSADIKVEEPAAQPED-SKTEVQATVAEISKEEKPSATDAE 539
E+P E+K ++ V+ +A+PE S+++ + T E + E KP T AE
Sbjct: 109 EVPPEESKR-EVVVQPESAKPETKSESKPETTKPETTSETKPE-TKAE 154
Score = 20.6 bits (41), Expect(2) = 2.6
Identities = 6/14 (42%), Positives = 10/14 (71%)
Frame = +3
Query: 288 IPEAKKDDIAPEDS 329
+PE K ++ PE+S
Sbjct: 102 VPEESKQEVPPEES 115
>At2g45800.1 68415.m05696 LIM domain-containing protein similar to
PGPS/D1 [Petunia x hybrida] GI:4105772, LIM domain
protein PLIM1 [Nicotiana tabacum] GI:5932418; contains
Pfam profile PF00412: LIM domain
Length = 226
Score = 28.7 bits (61), Expect = 3.8
Identities = 11/17 (64%), Positives = 14/17 (82%)
Frame = +3
Query: 273 DEVPAIPEAKKDDIAPE 323
D+ +IPEAK+DD APE
Sbjct: 197 DDTASIPEAKEDDAAPE 213
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 28.7 bits (61), Expect = 3.8
Identities = 22/72 (30%), Positives = 32/72 (44%), Gaps = 7/72 (9%)
Frame = +3
Query: 387 AKSSEIPDAEAKSADIKVEEPAAQPED-------SKTEVQATVAEISKEEKPSATDAEGS 545
A E P AE ++ VEE AAQPE + E + A K++K + E
Sbjct: 308 AALGETPAAERPASSTPVEEKAAQPEPVAPVENAGEKEGEEETAAAKKKKKKKEKEKEKK 367
Query: 546 ADSAAIIPNMVK 581
A +AA + V+
Sbjct: 368 AAAAAAATSSVE 379
>At1g03100.1 68414.m00285 pentatricopeptide (PPR) repeat-containing
protein low similarity to CRP1 [Zea mays] GI:3289002;
contains Pfam profile PF01535: PPR repeat
Length = 793
Score = 28.3 bits (60), Expect = 5.0
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +1
Query: 409 MLKQNLLILKWKNQLLSLKIQKLKYKLPSLKFQKKKNL 522
M K+N+ + K+K ++L LK K YK + K Q + L
Sbjct: 740 MEKKNMFVDKYKYRMLFLKYHKTAYKGKAPKVQSESQL 777
>At2g02170.1 68415.m00153 remorin family protein contains Pfam
domain, PF03763: Remorin, C-terminal region
Length = 486
Score = 27.9 bits (59), Expect = 6.6
Identities = 16/50 (32%), Positives = 27/50 (54%)
Frame = +3
Query: 408 DAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSA 557
+AE K ++KVE + +D + AT+ E EEK +A +A+ +A
Sbjct: 411 EAEMKKTEVKVERIKGRAQDRLMKKLATI-ERKAEEKRAAAEAKKDHQAA 459
>At4g25610.1 68417.m03689 zinc finger (C2H2 type) family protein
contains Pfam profile: PF00096 zinc finger, C2H2 type
Length = 586
Score = 27.5 bits (58), Expect = 8.8
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = -1
Query: 375 FLRFSLQERFPVSRYRSLLGLYRPFWPLV 289
FL F E PV ++ L L RP WPL+
Sbjct: 25 FLSFPRPENTPVQLFQLLHTLERPGWPLL 53
>At4g20260.2 68417.m02960 DREPP plasma membrane polypeptide family
protein contains Pfam profile: PF05558 DREPP plasma
membrane polypeptide
Length = 225
Score = 27.5 bits (58), Expect = 8.8
Identities = 16/45 (35%), Positives = 21/45 (46%), Gaps = 1/45 (2%)
Frame = +3
Query: 399 EIPDAEAKSADIKVEEPAAQPEDSKTE-VQATVAEISKEEKPSAT 530
E+ E ++K EEPA E +KTE EI +E K T
Sbjct: 133 EVKTKEIPVEEVKAEEPAKTEEPAKTEGTSGEKEEIVEETKKGET 177
>At4g20260.1 68417.m02959 DREPP plasma membrane polypeptide family
protein contains Pfam profile: PF05558 DREPP plasma
membrane polypeptide
Length = 225
Score = 27.5 bits (58), Expect = 8.8
Identities = 16/45 (35%), Positives = 21/45 (46%), Gaps = 1/45 (2%)
Frame = +3
Query: 399 EIPDAEAKSADIKVEEPAAQPEDSKTE-VQATVAEISKEEKPSAT 530
E+ E ++K EEPA E +KTE EI +E K T
Sbjct: 133 EVKTKEIPVEEVKAEEPAKTEEPAKTEGTSGEKEEIVEETKKGET 177
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,810,965
Number of Sequences: 28952
Number of extensions: 173076
Number of successful extensions: 542
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 529
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 542
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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