BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ceN-0531 (760 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC11E3.07 |vma4||V-type ATPase subunit E|Schizosaccharomyces p... 118 7e-28 SPBC3B9.09 |vps36||RBZ zinc finger protein Vps36|Schizosaccharom... 31 0.24 SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S... 28 1.3 SPBC14C8.04 |||acetolactate synthase regulatory unit|Schizosacch... 28 1.3 SPBC215.10 |||haloacid dehalogenase-like hydrolase|Schizosacchar... 27 2.9 SPBC27.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||M... 27 2.9 SPAC3H1.01c |orp3|orc3, SPAP14E8.06c|origin recognition complex ... 27 2.9 SPAC824.05 |vps16||HOPS complex subunit Vps16 |Schizosaccharomyc... 26 5.1 SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 26 5.1 SPAC637.06 |||alpha-1,2-galactosyltransferase |Schizosaccharomyc... 26 6.7 SPCC16C4.15 |rml2||mitochondrial ribosomal protein subunit L2|Sc... 26 6.7 >SPAC11E3.07 |vma4||V-type ATPase subunit E|Schizosaccharomyces pombe|chr 1|||Manual Length = 227 Score = 118 bits (285), Expect = 7e-28 Identities = 67/216 (31%), Positives = 113/216 (52%), Gaps = 1/216 (0%) Frame = +3 Query: 108 LSDADVQKQIKHMMAFIEQXXXXXXXXXXXXXXXXFNIEKGRLVQQQRLKIMXXXXXXXX 287 LSD VQ ++ M++FI+Q F +EK ++V++Q I Sbjct: 3 LSDEQVQAEMHKMVSFIKQEALEKAKEIHTLSEEEFQVEKAKIVREQCDAIDQTYDMKLK 62 Query: 288 XXXXXXXIQSSNMLNQARLKVLKVREDHVRNVLDEARKRLAEVPKDTKLYSELLVTLIVQ 467 I SN+LN++RL++L ++ + ++ K+L + + Y++ + LIVQ Sbjct: 63 RASMAQKIAKSNVLNKSRLEILNSKQKVIDDIFSRVEKKLDGIEQKKDAYTKFMADLIVQ 122 Query: 468 ALFQLMEPTVTIRVRQTDKALVESLLGKAQTDYKNKIKK-DVVLKVDTENFLSPDTCGGI 644 A+ L EP + RQ D +V++ + KA K+K D L +T++FL+ GG+ Sbjct: 123 AMELLGEPVGIVYSRQRDAEIVKAAIPKATEVLKSKNGSIDYELDAETDDFLNDSVLGGV 182 Query: 645 ELVAARGRIKISNTLESRLELIAQQLLPEIRNALFG 752 LV G+I++ NTL +RLE++ ++ LPEIR LFG Sbjct: 183 VLVGLGGKIRVDNTLRARLEIVKEEALPEIRRLLFG 218 >SPBC3B9.09 |vps36||RBZ zinc finger protein Vps36|Schizosaccharomyces pombe|chr 2|||Manual Length = 467 Score = 30.7 bits (66), Expect = 0.24 Identities = 15/41 (36%), Positives = 21/41 (51%), Gaps = 1/41 (2%) Frame = -2 Query: 636 HRCRATKSS-RCRLSTQHPS*SYSCSLFGLFRAGTPPEPCR 517 H R +SS + RLS +H S++C + G P PCR Sbjct: 77 HTSRYFRSSPKIRLSLRHVEKSWACKICTFINVGDPINPCR 117 >SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |Schizosaccharomyces pombe|chr 2|||Manual Length = 1944 Score = 28.3 bits (60), Expect = 1.3 Identities = 19/68 (27%), Positives = 33/68 (48%), Gaps = 2/68 (2%) Frame = +3 Query: 516 TDKALVESLLGKAQTDYKNKIKKDVVLKVDTENF--LSPDTCGGIELVAARGRIKISNTL 689 TD+ ++ + K + D + K+DV+ K+D S G +E +G +K S Sbjct: 1861 TDQTAADNKVTKPRLDESSSSKQDVLNKIDESEIEQASSKKPGYVEKNKDKGHMKKSKKP 1920 Query: 690 ESRLELIA 713 +S+L L A Sbjct: 1921 KSKLALAA 1928 >SPBC14C8.04 |||acetolactate synthase regulatory unit|Schizosaccharomyces pombe|chr 2|||Manual Length = 289 Score = 28.3 bits (60), Expect = 1.3 Identities = 16/45 (35%), Positives = 26/45 (57%), Gaps = 1/45 (2%) Frame = +2 Query: 17 TSFPEPVINFLALYRPFFVLVVLKISSSHGAQRCRCSE-TDQAHD 148 T+ P+ V NFL+L RP+ VL + +S + +E T++A D Sbjct: 233 TATPDRVDNFLSLLRPYGVLEACRTGTSAMTRAPHSNEVTEEAED 277 >SPBC215.10 |||haloacid dehalogenase-like hydrolase|Schizosaccharomyces pombe|chr 2|||Manual Length = 302 Score = 27.1 bits (57), Expect = 2.9 Identities = 15/42 (35%), Positives = 24/42 (57%) Frame = +3 Query: 366 DHVRNVLDEARKRLAEVPKDTKLYSELLVTLIVQALFQLMEP 491 D N L+EA+KRLA +P D E+ +T + F+++ P Sbjct: 181 DDDTNGLEEAKKRLAGIPSD-----EVALTQALPQTFEIIPP 217 >SPBC27.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 1052 Score = 27.1 bits (57), Expect = 2.9 Identities = 28/93 (30%), Positives = 40/93 (43%), Gaps = 9/93 (9%) Frame = +3 Query: 384 LDEARKRLAEVPKDTK-LYSELLVTLIVQALFQL---MEPTVTIRVR-----QTDKALVE 536 ++EA K KD K L +L T+ + + +E T+TI V DK E Sbjct: 745 VEEAEKDYQPSEKDDKSLIGGILSTVEMTQSTDVNDEIENTLTIPVEVEHSDNIDKGSEE 804 Query: 537 SLLGKAQTDYKNKIKKDVVLKVDTENFLSPDTC 635 + LGK D N + DV+ V E + D C Sbjct: 805 NDLGKEAVDEANNNQNDVLESVVVEQSTTTDDC 837 >SPAC3H1.01c |orp3|orc3, SPAP14E8.06c|origin recognition complex subunit Orp3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 690 Score = 27.1 bits (57), Expect = 2.9 Identities = 18/60 (30%), Positives = 30/60 (50%), Gaps = 1/60 (1%) Frame = -3 Query: 497 DSGFHELEESLHNKCDQQL*VQF-GVLWHFSQALASFVEYITYMIFTHFQYLQTSLVQHV 321 +S F +EESL K + F + + S ++ +E I Y I THF S+++H+ Sbjct: 241 ESLFTSIEESLSLKFGWRTRRFFRSMFYERSWSVERVIECIRYSILTHFYGNALSIIEHL 300 >SPAC824.05 |vps16||HOPS complex subunit Vps16 |Schizosaccharomyces pombe|chr 1|||Manual Length = 835 Score = 26.2 bits (55), Expect = 5.1 Identities = 16/42 (38%), Positives = 21/42 (50%) Frame = -3 Query: 692 LQSVADLDTSPGCNQLDSTTGVGRQKVLGVDFQHNILLDLIL 567 LQ V+ + SP L VG+ +V+ DF LLDL L Sbjct: 223 LQHVSHISISPNARYLALYESVGKVRVISSDFSKE-LLDLRL 263 >SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1|||Manual Length = 1841 Score = 26.2 bits (55), Expect = 5.1 Identities = 17/49 (34%), Positives = 25/49 (51%) Frame = -3 Query: 569 LVVCLGFSEQGLHQSLVGLTDADSDSGFHELEESLHNKCDQQL*VQFGV 423 L+V EQG ++ D+ +SG HE+ + L N DQQL Q + Sbjct: 492 LIVACEELEQGFDLDIL---DSLRESGIHEVIQLLRNFPDQQLEKQLNI 537 >SPAC637.06 |||alpha-1,2-galactosyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 347 Score = 25.8 bits (54), Expect = 6.7 Identities = 13/39 (33%), Positives = 21/39 (53%), Gaps = 1/39 (2%) Frame = -3 Query: 662 PGC-NQLDSTTGVGRQKVLGVDFQHNILLDLILVVCLGF 549 PG N LDS G R+K H + + ++L++C G+ Sbjct: 22 PGTSNVLDSKEGDTRRKYFTKTHLHRLFVFVVLLLCSGY 60 >SPCC16C4.15 |rml2||mitochondrial ribosomal protein subunit L2|Schizosaccharomyces pombe|chr 3|||Manual Length = 318 Score = 25.8 bits (54), Expect = 6.7 Identities = 13/41 (31%), Positives = 21/41 (51%), Gaps = 1/41 (2%) Frame = -2 Query: 414 LQPGAC-ELRRVHYVHDLHALSVPSDELGSACSKIGSSSEV 295 L+PG C LR + +HA+ V ++ C GSS+ + Sbjct: 162 LKPGNCFPLRLIPIGTVIHAIGVNPNQKAKLCRSAGSSARI 202 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,828,373 Number of Sequences: 5004 Number of extensions: 54346 Number of successful extensions: 169 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 160 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 168 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 363302114 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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