BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ceN-0493 (664 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g13950.1 68414.m01639 eukaryotic translation initiation facto... 201 4e-52 At1g69410.1 68414.m07972 eukaryotic translation initiation facto... 194 4e-50 At1g26630.1 68414.m03243 eukaryotic translation initiation facto... 190 9e-49 At3g06450.1 68416.m00746 anion exchange family protein similar t... 31 0.90 At1g56345.1 68414.m06477 pseudouridine synthase family protein l... 29 2.1 At5g14230.1 68418.m01663 ankyrin repeat family protein contains ... 29 2.8 At5g41460.1 68418.m05035 fringe-related protein strong similarit... 29 3.6 At5g03140.1 68418.m00262 lectin protein kinase family protein co... 29 3.6 At3g05830.1 68416.m00654 expressed protein 29 3.6 At3g53330.1 68416.m05884 plastocyanin-like domain-containing pro... 28 6.4 At3g49180.1 68416.m05375 transducin family protein / WD-40 repea... 28 6.4 At3g12240.1 68416.m01527 serine carboxypeptidase S10 family prot... 28 6.4 At3g06490.1 68416.m00753 myb family transcription factor (MYB108... 28 6.4 At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein... 28 6.4 At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-... 27 8.4 At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative simila... 27 8.4 At1g20860.1 68414.m02613 phosphate transporter family protein si... 27 8.4 >At1g13950.1 68414.m01639 eukaryotic translation initiation factor 5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Arabidopsis thaliana} Length = 158 Score = 201 bits (490), Expect = 4e-52 Identities = 87/150 (58%), Positives = 117/150 (78%), Gaps = 1/150 (0%) Frame = +1 Query: 58 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 237 E+ HFE+ D+GAS T+P Q +RKNG++++K RPCK+VE+STSKTGKHGHAK H V ID Sbjct: 4 EEHHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63 Query: 238 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGD-LG 414 IF KK EDI PS+HN DVPHV R DYQL DIS+DGY++L+ DNG ++DLK+P+ D L Sbjct: 64 IFTSKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLL 123 Query: 415 TQLRTDFDSGKELLCTVLKSCGEECVIAVK 504 Q+++ FD GK+L+ +V+ + GEE + A+K Sbjct: 124 QQIKSGFDDGKDLVVSVMSAMGEEQINALK 153 >At1g69410.1 68414.m07972 eukaryotic translation initiation factor 5A, putative / eIF-5A, putative strong similarity to eukaryotic initiation factor 5A (2) (Nicotiana plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Lycopersicon esculentum} Length = 158 Score = 194 bits (473), Expect = 4e-50 Identities = 85/150 (56%), Positives = 115/150 (76%), Gaps = 1/150 (0%) Frame = +1 Query: 58 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 237 ++ HFE+ D+GAS T+P Q +RK G +++KGRPCK+VE+STSKTGKHGHAK H V ID Sbjct: 4 DEHHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAID 63 Query: 238 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLG 414 IF KK EDI PS+HN DVPHV R DYQL DIS+DG+++L+ DNG ++DLK+P D L Sbjct: 64 IFTSKKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALL 123 Query: 415 TQLRTDFDSGKELLCTVLKSCGEECVIAVK 504 TQL+ F+ GK+++ +V+ + GEE + A+K Sbjct: 124 TQLKNGFEEGKDIVVSVMSAMGEEQMCALK 153 >At1g26630.1 68414.m03243 eukaryotic translation initiation factor 5A, putative / eIF-5A, putative strong similariy to SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Lycopersicon esculentum} Length = 159 Score = 190 bits (462), Expect = 9e-49 Identities = 83/150 (55%), Positives = 111/150 (74%), Gaps = 1/150 (0%) Frame = +1 Query: 58 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 237 ++ HFE +SGAS T+P +RK G +++K RPCK+VE+STSKTGKHGHAK H V ID Sbjct: 4 DEHHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63 Query: 238 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLG 414 IF KK EDI PS+HN DVPHV R DYQL DI++DG+++L+ D+G ++DLK+P D L Sbjct: 64 IFTAKKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLT 123 Query: 415 TQLRTDFDSGKELLCTVLKSCGEECVIAVK 504 Q+R FD GK+++ +V+ S GEE + AVK Sbjct: 124 AQMRLGFDEGKDIVVSVMSSMGEEQICAVK 153 >At3g06450.1 68416.m00746 anion exchange family protein similar to putative Anion exchanger family members: GB:AAD39673, GB:AAD55295 [Arabidopsis thaliana] Length = 732 Score = 30.7 bits (66), Expect = 0.90 Identities = 12/21 (57%), Positives = 13/21 (61%) Frame = -2 Query: 663 LRAPPLYYYSFFKLNLYTILC 601 LR PP Y+Y F L TILC Sbjct: 328 LRKPPAYHYDLFLLGFLTILC 348 >At1g56345.1 68414.m06477 pseudouridine synthase family protein low similarity to SP|P23851 Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate synthase) (Uracil hydrolyase) {Escherichia coli}; contains Pfam profile PF00849: RNA pseudouridylate synthase Length = 322 Score = 29.5 bits (63), Expect = 2.1 Identities = 20/69 (28%), Positives = 35/69 (50%), Gaps = 1/69 (1%) Frame = -2 Query: 468 QHGAQQFLAAVEVSTQLCAEVTIRDFEVLTQ-VTVVSHQGQVAIIRDISQLVVFALHVGY 292 +HGA + AA++V L +RD E + V+V S + + + D++ ++V G Sbjct: 176 KHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSVNSVKNESCELEDVNHVIVAE---GE 232 Query: 291 VHVVCGGTD 265 + CGG D Sbjct: 233 RELSCGGDD 241 >At5g14230.1 68418.m01663 ankyrin repeat family protein contains ankyrin repeats, Pfam:PF00023 Length = 591 Score = 29.1 bits (62), Expect = 2.8 Identities = 15/34 (44%), Positives = 16/34 (47%) Frame = +1 Query: 388 LKIPDGDLGTQLRTDFDSGKELLCTVLKSCGEEC 489 L IPDGD T L G +C L SCG C Sbjct: 412 LDIPDGDGYTPLMLAAREGHGHMCEYLISCGANC 445 >At5g41460.1 68418.m05035 fringe-related protein strong similarity to unknown protein (pir||T13026) similarity to predicted proteins + similar to hypothetical protein GB:AAC23643 [Arabidopsis thaliana] + weak similarity to Fringe [Schistocerca gregaria](GI:6573138);Fringe encodes an extracellular protein that regulates Notch signalling. Length = 524 Score = 28.7 bits (61), Expect = 3.6 Identities = 11/29 (37%), Positives = 16/29 (55%) Frame = +2 Query: 23 VVKFKTQQWVTSKTHTSRPETPGPQPPSP 109 ++ F T Q K++ S P +P P PP P Sbjct: 80 LILFHTNQTAVIKSYASPPPSPPPPPPPP 108 >At5g03140.1 68418.m00262 lectin protein kinase family protein contains Pfam domains, PF00138: Legume lectins alpha domain, PF00139: Legume lectins beta domain and PF00069: Protein kinase domain Length = 711 Score = 28.7 bits (61), Expect = 3.6 Identities = 13/36 (36%), Positives = 21/36 (58%) Frame = -3 Query: 158 RPFSITKPFLRRAEHCMGKVAEAPESPVSKCVSSMS 51 +P +T+P +R + A+ PE P++K SSMS Sbjct: 631 QPDPVTRPTMRSVVQILVGEADVPEVPIAKPSSSMS 666 >At3g05830.1 68416.m00654 expressed protein Length = 336 Score = 28.7 bits (61), Expect = 3.6 Identities = 24/82 (29%), Positives = 39/82 (47%) Frame = +1 Query: 211 AKVHLVGIDIFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDL 390 AK + G+D K ED+ P V+ +D ++T + D+ + LM +G + Sbjct: 186 AKAGIGGMDSELQKLLEDVSPMKFERMNRLVEVKDEEITKLKDE--IRLM--SGQWKHKT 241 Query: 391 KIPDGDLGTQLRTDFDSGKELL 456 K + L Q RTD D K++L Sbjct: 242 KELESQLEKQRRTDQDLKKKVL 263 >At3g53330.1 68416.m05884 plastocyanin-like domain-containing protein similar to mavicyanin SP:P80728 from [Cucurbita pepo] Length = 310 Score = 27.9 bits (59), Expect = 6.4 Identities = 13/18 (72%), Positives = 13/18 (72%), Gaps = 1/18 (5%) Frame = +2 Query: 56 SKTHT-SRPETPGPQPPS 106 SKTH SRP TP P PPS Sbjct: 127 SKTHERSRPITPSPPPPS 144 >At3g49180.1 68416.m05375 transducin family protein / WD-40 repeat family protein contains 5 WD-40 repeats (PF00400); GTP-binding protein beta chain homolog, Nicotiana tabacum, PIR:T16970 Length = 438 Score = 27.9 bits (59), Expect = 6.4 Identities = 10/20 (50%), Positives = 15/20 (75%) Frame = -2 Query: 516 SCVCFDGDDALLTAGFQHGA 457 +C+ F GDD+LL +G Q G+ Sbjct: 124 TCLVFSGDDSLLVSGSQDGS 143 >At3g12240.1 68416.m01527 serine carboxypeptidase S10 family protein contains Pfam profile: PF00450 serine carboxypeptidase; similar to serine carboxypeptidase I precursor (SP:P37890) [Oryza sativa] Length = 436 Score = 27.9 bits (59), Expect = 6.4 Identities = 20/76 (26%), Positives = 33/76 (43%), Gaps = 4/76 (5%) Frame = -2 Query: 375 VTVVSHQGQVAIIRDISQL---VVFALHVGYVHVVCGGTD-IFILFTIEDINPNQVNFSV 208 + ++ H +IIR + + F L GY+ V D +F F + NP + V Sbjct: 16 LVLIQHADSSSIIRYLPGFEGPLPFELETGYIGVGQKEEDQLFYYFIKSENNPEEDPLLV 75 Query: 207 AVLSGFGCGHFNNLAW 160 + G GC F+ L + Sbjct: 76 WLTGGPGCSSFSGLVY 91 >At3g06490.1 68416.m00753 myb family transcription factor (MYB108) identical to transcription factor MYB108 GI:15375290 from [Arabidopsis thaliana] Length = 323 Score = 27.9 bits (59), Expect = 6.4 Identities = 17/56 (30%), Positives = 27/56 (48%), Gaps = 2/56 (3%) Frame = +2 Query: 161 HARLLKCPHPKPESTATLKFTWLGLISSMVKSMKIS--VPPHTTWTYPT*SAKTTN 322 HA+ LKC + T+K+ W+ + ++S S TT T T SA T++ Sbjct: 122 HAKQLKCDVNSQQFKDTMKYLWMPRLVERIQSASASSAAAATTTTTTTTGSAGTSS 177 >At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein contains INTERPRO domain, IPR001878: Zn-finger CCHC type Length = 353 Score = 27.9 bits (59), Expect = 6.4 Identities = 15/45 (33%), Positives = 17/45 (37%), Gaps = 3/45 (6%) Frame = +2 Query: 59 KTHTSRPETPGPQPPS---PCNVRPCVKTVSLC*RVVHARLLKCP 184 KT T P PP PCN PC S + + R CP Sbjct: 22 KTTTKPTAAAAPSPPDIHCPCNAGPCNTVTSKTEKNPNRRFYTCP 66 >At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-box RNA helicase [Chlamydomonas reinhardtii] GI:12044832; contains Pfam profiles PF04408: Helicase associated domain (HA2), PF00271: Helicase conserved C-terminal domain Length = 1226 Score = 27.5 bits (58), Expect = 8.4 Identities = 15/51 (29%), Positives = 28/51 (54%), Gaps = 1/51 (1%) Frame = +1 Query: 301 VKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDL-GTQLRTDFDSGKE 450 V+R+ +++ Y L ADN + + G + GT+++T+FDS +E Sbjct: 350 VRRDGSKMSLAQSKKYSQLNADNAQWEDRQLLRSGAVRGTEVQTEFDSEEE 400 >At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative similar to SP|P35207 Antiviral protein SKI2 {Saccharomyces cerevisiae}; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 1347 Score = 27.5 bits (58), Expect = 8.4 Identities = 17/51 (33%), Positives = 30/51 (58%), Gaps = 6/51 (11%) Frame = +1 Query: 331 ISDDGYLTLMADNG--DLREDLKIPDGDLGTQLR----TDFDSGKELLCTV 465 +SD+ L + A G D+ +++ D DL Q++ + +SG+EL+CTV Sbjct: 1141 MSDEALLQMPAFQGRIDVLKNIGCIDDDLVVQIKGRVACEMNSGEELICTV 1191 >At1g20860.1 68414.m02613 phosphate transporter family protein similar to phosphate transporter [Catharanthus roseus] GI:2208908, inorganic phosphate transporter 1 [Solanum tuberosum] GI:1420871; contains Pfam profile PF00083: major facilitator superfamily protein Length = 534 Score = 27.5 bits (58), Expect = 8.4 Identities = 17/45 (37%), Positives = 20/45 (44%) Frame = +2 Query: 35 KTQQWVTSKTHTSRPETPGPQPPSPCNVRPCVKTVSLC*RVVHAR 169 K Q V S++H S T P PP P P K S C +H R Sbjct: 243 KDMQRVMSRSHISDEATTDPPPPPP---PPSYKLFSRCFFRLHGR 284 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,884,281 Number of Sequences: 28952 Number of extensions: 328002 Number of successful extensions: 1062 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 974 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1047 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1393347168 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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