Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-0473
(698 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 211 8e-56
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 196 3e-51
SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce... 28 1.5
SPAC23H3.05c |swd1||COMPASS complex subunit Swd1|Schizosaccharom... 27 2.0
SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb... 27 2.6
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 27 2.6
SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S... 27 3.4
SPAC30D11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 25 7.9
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 211 bits (515), Expect = 8e-56
Identities = 98/134 (73%), Positives = 115/134 (85%)
Frame = +2
Query: 62 LLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDAFEISKAK 241
+L+KHLIP A++ ESKVFY KMKGDYYRYLAE A GE R + S + Y+ A EI+ A+
Sbjct: 105 VLEKHLIPNAASAESKVFYYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEIATAE 164
Query: 242 MQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLIMQ 421
+ PTHPIRLGLALNFSVFYYEILNSPD+AC LAKQAFD+AI+ELD+L+E+SYKDSTLIMQ
Sbjct: 165 LAPTHPIRLGLALNFSVFYYEILNSPDRACYLAKQAFDEAISELDSLSEESYKDSTLIMQ 224
Query: 422 LLRDNLTLWTSDTQ 463
LLRDNLTLWTSD +
Sbjct: 225 LLRDNLTLWTSDAE 238
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 196 bits (477), Expect = 3e-51
Identities = 91/134 (67%), Positives = 110/134 (82%)
Frame = +2
Query: 62 LLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDAFEISKAK 241
+L+KHLIP A+ ESKVFY KMKGDYYRYLAE GE + S +AY+ A +I+ A+
Sbjct: 104 VLEKHLIPAATTGESKVFYYKMKGDYYRYLAEFTVGEVCKEAADSSLEAYKAASDIAVAE 163
Query: 242 MQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLIMQ 421
+ PT P+RLGLALNFSVFYYEIL+SP+ AC LAKQ FD+AI+ELD+L+E+SYKDSTLIMQ
Sbjct: 164 LPPTDPMRLGLALNFSVFYYEILDSPESACHLAKQVFDEAISELDSLSEESYKDSTLIMQ 223
Query: 422 LLRDNLTLWTSDTQ 463
LLRDNLTLWTSD +
Sbjct: 224 LLRDNLTLWTSDAE 237
>SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 817
Score = 27.9 bits (59), Expect = 1.5
Identities = 34/134 (25%), Positives = 53/134 (39%), Gaps = 4/134 (2%)
Frame = +2
Query: 47 FAFQGLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDS--QKAYQDA 220
F F L+K IP + + VF K K R LA R + + Q+ D
Sbjct: 366 FCFTPNLEKLTIPYSVTEAANVFQNKTK----RTLAVEQLLSERELLRRFTLQQRLVADL 421
Query: 221 FEISKAKMQPTHPIRL-GLALNF-SVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDS 394
E + P H L F S Y ++ D QLA Q F +++ L ++
Sbjct: 422 HEFYNSVKGPAHSFETEDPVLKFVSQSYDDLFPYMDNFIQLAVQLFYHISKKVNCLYVEA 481
Query: 395 YKDSTLIMQLLRDN 436
+ S ++Q ++DN
Sbjct: 482 FSSSDAVLQRIQDN 495
>SPAC23H3.05c |swd1||COMPASS complex subunit
Swd1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 398
Score = 27.5 bits (58), Expect = 2.0
Identities = 17/44 (38%), Positives = 25/44 (56%), Gaps = 1/44 (2%)
Frame = -2
Query: 616 LASASVMIPRQTTFSNVTRFLI-NTDENKCVCWGECGEILVVAA 488
L + SV+I +TFS V+R L +T + VCW C L+ A+
Sbjct: 43 LVNGSVVIWDLSTFS-VSRVLTGHTRAIQSVCWSSCDRFLLTAS 85
>SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1207
Score = 27.1 bits (57), Expect = 2.6
Identities = 17/51 (33%), Positives = 29/51 (56%), Gaps = 2/51 (3%)
Frame = +2
Query: 143 RYLAEVATG-ETRHSVVEDSQKAYQDAFEISKAKMQPTHP-IRLGLALNFS 289
RY+ ++ E R ED+++A + ++ + A +QP H I L LA+N S
Sbjct: 1049 RYIVALSCELERRRLGEEDTKRALELSYYFASADLQPMHSIIALRLAINAS 1099
>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1778
Score = 27.1 bits (57), Expect = 2.6
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 3/49 (6%)
Frame = +2
Query: 62 LLDKHLIPKASNPESKVFYLKMKGDYYR---YLAEVATGETRHSVVEDS 199
+++K IP++ E+K Y + GD+ +L E A E H V+ D+
Sbjct: 1608 MIEKLCIPESWLNEAKALYARYVGDHLNELYFLQEAALYEDAHKVLLDT 1656
>SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 971
Score = 26.6 bits (56), Expect = 3.4
Identities = 16/54 (29%), Positives = 26/54 (48%), Gaps = 1/54 (1%)
Frame = +2
Query: 41 LFFAFQGLL-DKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDS 199
L++ F+ L+ + H SNP S K KG++ Y A G ++ +DS
Sbjct: 213 LYWDFKPLVFNLHTSKTYSNPSSFATRKKEKGEHLSYAEAAAVGTQAKNIKKDS 266
>SPAC30D11.02c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 85
Score = 25.4 bits (53), Expect = 7.9
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = -2
Query: 253 CGLHFRLADFKSILVCFL*ILYNRMSGFSCGHFC 152
C L+F L + K+ L+ I+Y + GFS H C
Sbjct: 46 CLLNFSLRENKNYLI----IVYLPIEGFSANHMC 75
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,721,060
Number of Sequences: 5004
Number of extensions: 54294
Number of successful extensions: 166
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 166
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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