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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ceN-0473
         (698 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.)             183   1e-46
SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0)                     181   6e-46
SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.)              52   4e-07
SB_23213| Best HMM Match : SH3_1 (HMM E-Value=9.2e-12)                 31   0.90 
SB_13504| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   4.8  
SB_3346| Best HMM Match : Spectrin (HMM E-Value=0.012)                 28   8.4  

>SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 309

 Score =  183 bits (446), Expect = 1e-46
 Identities = 95/149 (63%), Positives = 110/149 (73%), Gaps = 4/149 (2%)
 Frame = +2

Query: 62  LLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDAFEISKA- 238
           LL+  LIP A + ESKVFYLKMKGDYYRY  EVA  + R  VV+ + KAY +A EI++  
Sbjct: 79  LLESKLIPNAQSTESKVFYLKMKGDYYRYEGEVAGADRRREVVQKAMKAYSEAQEIAEKD 138

Query: 239 -KMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLI 415
            K+ PT PIRLGLALNFSVFYYEI+    +AC LAK+AFDDAIAELDTL+ED YKDSTLI
Sbjct: 139 PKLPPTDPIRLGLALNFSVFYYEIVEDSKQACDLAKKAFDDAIAELDTLSEDQYKDSTLI 198

Query: 416 MQLLRDNLTLWTSDTQG--DGDEPAEGGD 496
           MQLLRDNLT+     Q   +  E AE GD
Sbjct: 199 MQLLRDNLTVVEKALQAYKEAKEAAETGD 227



 Score =  138 bits (335), Expect = 3e-33
 Identities = 69/103 (66%), Positives = 86/103 (83%), Gaps = 4/103 (3%)
 Frame = +2

Query: 182 SVVEDSQKAYQDAFEISKA---KMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAF 352
           +VVE + +AY++A E ++    K+ PT PIRLGLALNFSVF+YEI  + ++AC+LAKQAF
Sbjct: 207 TVVEKALQAYKEAKEAAETGDGKLAPTDPIRLGLALNFSVFHYEIQENQEEACKLAKQAF 266

Query: 353 DDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDTQGD-GDE 478
           DDAIAELD+LNED YKDSTLIMQLLRDNLTLW+S+ Q D GD+
Sbjct: 267 DDAIAELDSLNEDQYKDSTLIMQLLRDNLTLWSSENQEDQGDD 309


>SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0)
          Length = 248

 Score =  181 bits (440), Expect = 6e-46
 Identities = 84/128 (65%), Positives = 104/128 (81%)
 Frame = +2

Query: 95  NPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGL 274
           N E+KVFYLKM+GDY+RYL EVA G++R   +E S++AY+DA      ++ P+HPIRLGL
Sbjct: 120 NTEAKVFYLKMRGDYHRYLVEVAEGDSRKENIEKSREAYKDA-SAKAEELSPSHPIRLGL 178

Query: 275 ALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTS 454
           ALNFSVFYYEI N P +AC+LAK+AFDDAIA LD L ++SYKDSTLIMQLLRDNLTLWTS
Sbjct: 179 ALNFSVFYYEIENKPPEACKLAKEAFDDAIAVLDNLKDESYKDSTLIMQLLRDNLTLWTS 238

Query: 455 DTQGDGDE 478
           +   +G +
Sbjct: 239 EQDQEGQD 246


>SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 219

 Score = 52.0 bits (119), Expect = 4e-07
 Identities = 23/38 (60%), Positives = 31/38 (81%)
 Frame = +2

Query: 59  GLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGE 172
           G+L++ LIP A + E+KVFY K+KGDYYRYLAE + G+
Sbjct: 179 GILER-LIPGAEDEENKVFYFKLKGDYYRYLAEFSHGQ 215


>SB_23213| Best HMM Match : SH3_1 (HMM E-Value=9.2e-12)
          Length = 979

 Score = 31.1 bits (67), Expect = 0.90
 Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 3/49 (6%)
 Frame = +2

Query: 353 DDAIAELDTLNEDSYKDSTLIMQL---LRDNLTLWTSDTQGDGDEPAEG 490
           DD   + D  ++DS  + +   QL   L+  + LW S TQGD D  A G
Sbjct: 850 DDDDFDTDEWSDDSDAEGSAAGQLKLCLKREILLWKSGTQGDADRRATG 898


>SB_13504| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 4924

 Score = 28.7 bits (61), Expect = 4.8
 Identities = 19/53 (35%), Positives = 30/53 (56%), Gaps = 1/53 (1%)
 Frame = +2

Query: 161  ATGETRHS-VVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNS 316
            A GETR    + D+ +A +D     KA+ Q     +   ALN S+F++E++NS
Sbjct: 4020 ALGETRRKRSLVDTPEANED-----KAQHQVPDAEKCQFALNASLFFFEVINS 4067


>SB_3346| Best HMM Match : Spectrin (HMM E-Value=0.012)
          Length = 983

 Score = 27.9 bits (59), Expect = 8.4
 Identities = 17/58 (29%), Positives = 27/58 (46%)
 Frame = +2

Query: 296 YYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDTQGD 469
           YY I  +  K      Q FDD    +   +ED+ +D+T  ++L+       T+D  GD
Sbjct: 505 YYNIPYNKPKKNDFKLQLFDDYKQAVTQASEDTLRDATTAIELMNAP----TTDVHGD 558


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,740,264
Number of Sequences: 59808
Number of extensions: 420012
Number of successful extensions: 1242
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 1141
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1240
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1829596184
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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