BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ceN-0410 (543 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC4C3.07 |||translation initiation factor eIF3f|Schizosaccharo... 99 4e-22 SPCC1682.10 |rpn8||19S proteasome regulatory subunit Rpn8|Schizo... 71 1e-13 SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 27 2.4 SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr... 26 3.1 SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc... 26 4.1 SPBC16D10.07c |sir2||Sir2 family histone deacetylase Sir2|Schizo... 25 5.5 >SPBC4C3.07 |||translation initiation factor eIF3f|Schizosaccharomyces pombe|chr 2|||Manual Length = 302 Score = 99.1 bits (236), Expect = 4e-22 Identities = 58/172 (33%), Positives = 92/172 (53%), Gaps = 15/172 (8%) Frame = +2 Query: 2 VHPVVLFQIVDAYERRNADSHRVIGTLLGT--SDKGVVEVTNCFCVPHKEHADQVEAELN 175 + P VLF I+D R++ ++ RVIGTLLGT D +E+ +CF VPH E ++QVE E+ Sbjct: 25 IEPAVLFSILDHSTRKSENNQRVIGTLLGTRSEDGREIEIKSCFAVPHNESSEQVEVEME 84 Query: 176 YAMDVYELNRRVNSSESIVGWWATGNEVTNHSSVIHEYYSRECREP------------VH 319 Y +Y L+ + N E +VGW+AT ++ S++I Y+ EP VH Sbjct: 85 YHRAMYHLHLKANPREVVVGWYATSPDLDAFSALIQNLYASPA-EPGTAPLGTYPHPCVH 143 Query: 320 VTLDTSLAGGRMGLRAYVCVPLGVPNG-KQGCMFTPVDVTLTCYEPEIVGLQ 472 +T++T ++ + ++ YV P+G+ C F P T+ E GL+ Sbjct: 144 LTVNTDVS-SPLAIKTYVSSPVGITERLADSCAFVPTPFTIRDDEAVRSGLK 194 >SPCC1682.10 |rpn8||19S proteasome regulatory subunit Rpn8|Schizosaccharomyces pombe|chr 3|||Manual Length = 324 Score = 70.9 bits (166), Expect = 1e-13 Identities = 43/162 (26%), Positives = 76/162 (46%), Gaps = 5/162 (3%) Frame = +2 Query: 2 VHPVVLFQIVDAYERR-NADSHRVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVEA---E 169 VHP+VL VD+Y R RV+G LLG ++ VV V N + +P +E + Sbjct: 19 VHPLVLLSAVDSYNRSAKGTKRRVVGILLGQNNGDVVNVANSYAIPFEEDEKNASVWFLD 78 Query: 170 LNYAMDVYELNRRVNSSESIVGWWATGNEVTNHSSVIHEYYSRECREPVHVTLDTSLAGG 349 N+ + E+ +++N++E +VGW+ TG ++ I+ + PV V +D Sbjct: 79 HNFMESMNEMFKKINANEKLVGWYHTGPQLRPSDLEINNLLKKYIPNPVLVIIDVKPKSV 138 Query: 350 RMGLRAYVCVPLGVPNG-KQGCMFTPVDVTLTCYEPEIVGLQ 472 + AY + +G K F + ++ E E +G++ Sbjct: 139 GLPTNAYFAIDEIEDDGSKSSRTFVHLPSSIEAEEAEEIGVE 180 >SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1334 Score = 26.6 bits (56), Expect = 2.4 Identities = 13/42 (30%), Positives = 21/42 (50%) Frame = +2 Query: 38 YERRNADSHRVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVE 163 Y+ R DSHR I L GT + + + + +K H ++E Sbjct: 483 YDHRLRDSHREIYQLQGTGYRPFLRANDNASINNKNHHKELE 524 >SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 131 Score = 26.2 bits (55), Expect = 3.1 Identities = 6/11 (54%), Positives = 10/11 (90%) Frame = +1 Query: 412 HVHSCRCHSHV 444 H+H CRCH+++ Sbjct: 69 HIHCCRCHTYI 79 >SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc25|Schizosaccharomyces pombe|chr 1|||Manual Length = 596 Score = 25.8 bits (54), Expect = 4.1 Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 1/49 (2%) Frame = +1 Query: 49 KCRFSQSYRHLIGHERQRSGG-SNQLLLRATQRTCRSSRSGT*LRDGCL 192 + R S SY I ++ SGG + + L A RTC S + T L + CL Sbjct: 201 RSRSSSSYS--INKKKGTSGGQATRHLTYALSRTCSQSSNTTSLLESCL 247 >SPBC16D10.07c |sir2||Sir2 family histone deacetylase Sir2|Schizosaccharomyces pombe|chr 2|||Manual Length = 475 Score = 25.4 bits (53), Expect = 5.5 Identities = 13/27 (48%), Positives = 17/27 (62%) Frame = +3 Query: 219 LKVLLVGGRLAMK*PTTPLLYTSITPV 299 LKV V +++ PTTP +Y S TPV Sbjct: 376 LKVAPVSELISVIPPTTPQIYISRTPV 402 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,405,278 Number of Sequences: 5004 Number of extensions: 50482 Number of successful extensions: 148 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 141 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 146 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 223909422 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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