Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-0397
(754 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g48920.1 68414.m05480 nucleolin, putative similar to nuM1 pro... 33 0.15
At5g26200.1 68418.m03118 mitochondrial substrate carrier family ... 32 0.47
At3g49990.1 68416.m05466 expressed protein 31 0.62
At5g63550.1 68418.m07976 expressed protein 31 1.1
At1g22530.1 68414.m02814 SEC14 cytosolic factor family protein /... 30 1.4
At1g62480.1 68414.m07050 vacuolar calcium-binding protein-relate... 29 2.5
At3g10660.1 68416.m01282 calcium-dependent protein kinase isofor... 27 3.0
At2g45800.1 68415.m05696 LIM domain-containing protein similar t... 29 4.4
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 29 4.4
At1g03100.1 68414.m00285 pentatricopeptide (PPR) repeat-containi... 28 5.8
At2g02170.1 68415.m00153 remorin family protein contains Pfam do... 28 7.7
>At1g48920.1 68414.m05480 nucleolin, putative similar to nuM1
protein GI:1279562 from [Medicago sativa]
Length = 557
Score = 33.5 bits (73), Expect = 0.15
Identities = 15/51 (29%), Positives = 28/51 (54%)
Frame = +2
Query: 413 PDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSA 565
P A AK+ +K ++ ++ +DS +E + +K KP+A D+ S D +
Sbjct: 141 PAAAAKNGSVKAKKESSSEDDSSSEDEPAKKPAAKIAKPAAKDSSSSDDDS 191
>At5g26200.1 68418.m03118 mitochondrial substrate carrier family
protein contains Pfam profile: PF00153 mitochondrial
carrier protein
Length = 342
Score = 31.9 bits (69), Expect = 0.47
Identities = 17/35 (48%), Positives = 24/35 (68%)
Frame = -2
Query: 219 LTESSILGATSRIG*AGTTSFSSATGIAKLTAAKA 115
+T+SS+ AT R+G + TTS + A G A LT+A A
Sbjct: 112 ITKSSVGQATVRLGLSDTTSLAVANGAAGLTSAVA 146
>At3g49990.1 68416.m05466 expressed protein
Length = 502
Score = 31.5 bits (68), Expect = 0.62
Identities = 17/46 (36%), Positives = 25/46 (54%)
Frame = +2
Query: 410 IPDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAE 547
+P A+ D+K E P A+P KT Q + E K+E+ +A AE
Sbjct: 422 LPGRRAEQTDVKAEIPKAEPIKRKTHGQESKEE--KKERKNAVKAE 465
>At5g63550.1 68418.m07976 expressed protein
Length = 530
Score = 30.7 bits (66), Expect = 1.1
Identities = 18/67 (26%), Positives = 34/67 (50%)
Frame = +2
Query: 407 EIPDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSAAIIPNMV 586
E D + + +++VE+P+ + SK V+ + K+++PS A+GSA S +
Sbjct: 320 EKTDTDDEKDEVEVEKPSKKKSSSKKTVEESSGSKGKDKQPS---AKGSARSGEKSSKQI 376
Query: 587 KKIDLAP 607
K +P
Sbjct: 377 AKSTSSP 383
>At1g22530.1 68414.m02814 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus; similar to SEC14-like protein 2
(Alpha-tocopherol associated protein) (TAP) (bTAP)
(Fragment) (SP:P58875) {Bos taurus}
Length = 683
Score = 30.3 bits (65), Expect = 1.4
Identities = 16/41 (39%), Positives = 23/41 (56%)
Frame = +2
Query: 413 PDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSA 535
P E K + K EE + E+ KTE ++ AE +EEK +A
Sbjct: 123 PVKEEKVEEKKTEETEEKKEEVKTEEKSLEAETKEEEKSAA 163
>At1g62480.1 68414.m07050 vacuolar calcium-binding protein-related
contains weak similarity to vacuolar calcium binding
protein [Raphanus sativus] gi|9049359|dbj|BAA99394
Length = 152
Score = 29.5 bits (63), Expect = 2.5
Identities = 21/83 (25%), Positives = 38/83 (45%), Gaps = 2/83 (2%)
Frame = +2
Query: 284 EVPAIPEAKKDDIAPEDSDIAXXXXXXXXXXXXXXXXAKSSEIPDAEAKSADIKV-EEPA 460
E P + E KKD+ AP ++ + K E+ + + + A + V EE
Sbjct: 73 ETPEVVEIKKDEEAPVETPVV---VEDESKTEEVVEAKKEEEVEEKKTEEAPVVVEEEKK 129
Query: 461 AQPEDSKTEVQATV-AEISKEEK 526
+ E+ K V+A+V A + K ++
Sbjct: 130 PEAEEEKPAVEASVTAPVEKADE 152
>At3g10660.1 68416.m01282 calcium-dependent protein kinase isoform 2
(CPK2) identical to calcium-dependent protein kinase
isoform 2 [Arabidopsis thaliana] gi|9837343|gb|AAG00535;
contains protein kinase domain, Pfam:PF00069; contains
EF hand domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 646
Score = 27.1 bits (57), Expect(2) = 3.0
Identities = 17/48 (35%), Positives = 29/48 (60%), Gaps = 1/48 (2%)
Frame = +2
Query: 407 EIPDAEAKSADIKVEEPAAQPED-SKTEVQATVAEISKEEKPSATDAE 547
E+P E+K ++ V+ +A+PE S+++ + T E + E KP T AE
Sbjct: 109 EVPPEESKR-EVVVQPESAKPETKSESKPETTKPETTSETKPE-TKAE 154
Score = 20.6 bits (41), Expect(2) = 3.0
Identities = 6/14 (42%), Positives = 10/14 (71%)
Frame = +2
Query: 296 IPEAKKDDIAPEDS 337
+PE K ++ PE+S
Sbjct: 102 VPEESKQEVPPEES 115
>At2g45800.1 68415.m05696 LIM domain-containing protein similar to
PGPS/D1 [Petunia x hybrida] GI:4105772, LIM domain
protein PLIM1 [Nicotiana tabacum] GI:5932418; contains
Pfam profile PF00412: LIM domain
Length = 226
Score = 28.7 bits (61), Expect = 4.4
Identities = 11/17 (64%), Positives = 14/17 (82%)
Frame = +2
Query: 281 DEVPAIPEAKKDDIAPE 331
D+ +IPEAK+DD APE
Sbjct: 197 DDTASIPEAKEDDAAPE 213
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 28.7 bits (61), Expect = 4.4
Identities = 22/72 (30%), Positives = 32/72 (44%), Gaps = 7/72 (9%)
Frame = +2
Query: 395 AKSSEIPDAEAKSADIKVEEPAAQPED-------SKTEVQATVAEISKEEKPSATDAEGS 553
A E P AE ++ VEE AAQPE + E + A K++K + E
Sbjct: 308 AALGETPAAERPASSTPVEEKAAQPEPVAPVENAGEKEGEEETAAAKKKKKKKEKEKEKK 367
Query: 554 ADSAAIIPNMVK 589
A +AA + V+
Sbjct: 368 AAAAAAATSSVE 379
>At1g03100.1 68414.m00285 pentatricopeptide (PPR) repeat-containing
protein low similarity to CRP1 [Zea mays] GI:3289002;
contains Pfam profile PF01535: PPR repeat
Length = 793
Score = 28.3 bits (60), Expect = 5.8
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +3
Query: 417 MLKQNLLILKWKNQLLSLKIQKLKYKLPSLKFQKKKNL 530
M K+N+ + K+K ++L LK K YK + K Q + L
Sbjct: 740 MEKKNMFVDKYKYRMLFLKYHKTAYKGKAPKVQSESQL 777
>At2g02170.1 68415.m00153 remorin family protein contains Pfam
domain, PF03763: Remorin, C-terminal region
Length = 486
Score = 27.9 bits (59), Expect = 7.7
Identities = 16/50 (32%), Positives = 27/50 (54%)
Frame = +2
Query: 416 DAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSA 565
+AE K ++KVE + +D + AT+ E EEK +A +A+ +A
Sbjct: 411 EAEMKKTEVKVERIKGRAQDRLMKKLATI-ERKAEEKRAAAEAKKDHQAA 459
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,214,380
Number of Sequences: 28952
Number of extensions: 198458
Number of successful extensions: 559
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 547
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 559
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1672953192
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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