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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ceN-0371
         (703 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_56549| Best HMM Match : Spermine_synth (HMM E-Value=1.5e-29)        81   6e-16
SB_55793| Best HMM Match : Spermine_synth (HMM E-Value=1.6e-15)        73   2e-13
SB_45640| Best HMM Match : Epimerase (HMM E-Value=9.4e-38)             36   0.042
SB_26979| Best HMM Match : THF_DHG_CYH_C (HMM E-Value=0.017)           36   0.042
SB_56512| Best HMM Match : F5_F8_type_C (HMM E-Value=0)                29   3.6  
SB_43221| Best HMM Match : PHF5 (HMM E-Value=2)                        29   4.8  
SB_25716| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   4.8  
SB_25563| Best HMM Match : HEAT (HMM E-Value=2.5e-20)                  29   4.8  
SB_8718| Best HMM Match : Lipase_GDSL (HMM E-Value=0.023)              29   4.8  
SB_21644| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   6.4  

>SB_56549| Best HMM Match : Spermine_synth (HMM E-Value=1.5e-29)
          Length = 294

 Score = 81.4 bits (192), Expect = 6e-16
 Identities = 35/48 (72%), Positives = 40/48 (83%)
 Frame = +2

Query: 464 SLGKVLVLDGIIQCTQKDEFSYQEMISFLPLCCHKNPENVLIVGGGDG 607
           S G VLVLDG+IQCT +DEFSYQEMIS LPL  H NP+ VL++GGGDG
Sbjct: 157 SFGTVLVLDGVIQCTDRDEFSYQEMISMLPLNSHPNPKKVLVIGGGDG 204



 Score = 35.5 bits (78), Expect = 0.042
 Identities = 15/27 (55%), Positives = 20/27 (74%)
 Frame = +1

Query: 622 EVAKHPQVKHIVLVEIDDRVIELSKRY 702
           E +KHP V+ IV  EID+ VI +SK+Y
Sbjct: 209 EASKHPSVESIVQCEIDEDVINISKKY 235


>SB_55793| Best HMM Match : Spermine_synth (HMM E-Value=1.6e-15)
          Length = 317

 Score = 72.9 bits (171), Expect = 2e-13
 Identities = 33/72 (45%), Positives = 47/72 (65%)
 Frame = +2

Query: 392 SFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTQKDEFSYQEMISFLPLCCHKN 571
           SFE+ EVL  +++   ++ +F     G V+ LDGIIQ T++DEF Y EM++  PL  H  
Sbjct: 194 SFEIGEVLFEQQTDSWHLIIFRNEEFGTVMALDGIIQTTERDEFIYHEMLTHTPLFAHGA 253

Query: 572 PENVLIVGGGDG 607
            + VLI+GGGDG
Sbjct: 254 AKRVLIIGGGDG 265



 Score = 37.9 bits (84), Expect = 0.008
 Identities = 15/27 (55%), Positives = 20/27 (74%)
 Frame = +1

Query: 622 EVAKHPQVKHIVLVEIDDRVIELSKRY 702
           EV KHP+V+H+  VEID  VI++ K Y
Sbjct: 270 EVLKHPEVEHVTQVEIDQSVIDMCKTY 296


>SB_45640| Best HMM Match : Epimerase (HMM E-Value=9.4e-38)
          Length = 451

 Score = 35.5 bits (78), Expect = 0.042
 Identities = 21/74 (28%), Positives = 38/74 (51%)
 Frame = +1

Query: 316 KQEKDGQTKNQLVYGIMRYVAWGYFLIRSQRSTAHRKIKIPEYPSF*YNQFGEGSCLGRN 495
           K+    QTK   V  ++R     YF+I+ +  T  R++++P+ PSF  N   + +   + 
Sbjct: 46  KKSGADQTKRH-VLALLRAAFAIYFVIQIKERTVRRELEMPDQPSFDNNNLVQNTICSQE 104

Query: 496 HTMHSER*VFLSGN 537
           HT +     +L+GN
Sbjct: 105 HTANGLS--YLNGN 116


>SB_26979| Best HMM Match : THF_DHG_CYH_C (HMM E-Value=0.017)
          Length = 453

 Score = 35.5 bits (78), Expect = 0.042
 Identities = 22/71 (30%), Positives = 36/71 (50%), Gaps = 4/71 (5%)
 Frame = +2

Query: 371 MWPGGTFSFEVKEVLHTEKSKYQNIQVF----DTTSLGKVLVLDGIIQCTQKDEFSYQEM 538
           +WPG   +  +K++L +EKS  Q  +VF    D T  GK +    +   T   +  YQ M
Sbjct: 132 LWPGPVSTEAIKDMLKSEKSS-QMQRVFADSEDVTFTGKAVA--WLAAATDPSQCVYQLM 188

Query: 539 ISFLPLCCHKN 571
           ++  P+C + N
Sbjct: 189 VNHRPMCVYSN 199


>SB_56512| Best HMM Match : F5_F8_type_C (HMM E-Value=0)
          Length = 1219

 Score = 29.1 bits (62), Expect = 3.6
 Identities = 20/86 (23%), Positives = 43/86 (50%), Gaps = 1/86 (1%)
 Frame = +3

Query: 405 KKYCTQKNQNTRISKFLIQPVWGRFLSWTESYNALRKMSFLIRK*YHFCLYAATK-IQKM 581
           K +   +++NT +S  L +P++ RF+     + +       +R  +H C    T+ +  +
Sbjct: 623 KYFIGNRDRNTVVSSVLEEPIYPRFV---RIHPSKWYQHISLRLEFHGCRNGFTQPVMPL 679

Query: 582 YS*SVEVMGGVARGSRQTSSSETHCS 659
            S ++ +  G+ + S+ T+SSE   S
Sbjct: 680 CSSALGLQSGLIKDSQMTASSELSSS 705


>SB_43221| Best HMM Match : PHF5 (HMM E-Value=2)
          Length = 361

 Score = 28.7 bits (61), Expect = 4.8
 Identities = 12/33 (36%), Positives = 17/33 (51%)
 Frame = +2

Query: 269 TCLILDFNCRLGTNY*SKKKMDKLKTNWFTESC 367
           T L+L+ + + G  Y  K+K  K    WF E C
Sbjct: 207 TKLLLEISEKAGFKYGDKQKCQKTNAPWFDEEC 239


>SB_25716| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 124

 Score = 28.7 bits (61), Expect = 4.8
 Identities = 9/17 (52%), Positives = 13/17 (76%)
 Frame = +2

Query: 593 GGGDGRCRKGKSPNILK 643
           GGG+G+C+KGK   + K
Sbjct: 9   GGGEGKCKKGKDEGVCK 25


>SB_25563| Best HMM Match : HEAT (HMM E-Value=2.5e-20)
          Length = 1803

 Score = 28.7 bits (61), Expect = 4.8
 Identities = 17/56 (30%), Positives = 28/56 (50%), Gaps = 1/56 (1%)
 Frame = -1

Query: 622 PLATPPITSTDYEYIFW-IFVAA*RQK*YHFLIRKLIFLSALYDSVQDKNLPQTGC 458
           P    PI   D E I   +   A  +  Y +++ K   ++AL+DS+QD+ L +  C
Sbjct: 573 PFLKQPIIQVDQEVILVSVLKEAINRSVYDYIV-KSTHIAALFDSLQDRQLMRNVC 627


>SB_8718| Best HMM Match : Lipase_GDSL (HMM E-Value=0.023)
          Length = 836

 Score = 28.7 bits (61), Expect = 4.8
 Identities = 12/33 (36%), Positives = 17/33 (51%)
 Frame = +2

Query: 269 TCLILDFNCRLGTNY*SKKKMDKLKTNWFTESC 367
           T L+L+ + + G  Y  K+K  K    WF E C
Sbjct: 478 TKLLLEISEKAGFKYGDKQKCQKTNAPWFDEEC 510


>SB_21644| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 787

 Score = 28.3 bits (60), Expect = 6.4
 Identities = 18/56 (32%), Positives = 26/56 (46%)
 Frame = +2

Query: 386 TFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTQKDEFSYQEMISFLP 553
           TF  ++K      + KY N  V  TTS G V   D ++ CT        +M+ F+P
Sbjct: 170 TFKDKIKLNETVARVKYNNTGVEVTTSSGDVYSADYVV-CTFSTGVLASDMVEFVP 224


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,867,875
Number of Sequences: 59808
Number of extensions: 462071
Number of successful extensions: 1130
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1050
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1130
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1841633001
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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