Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-0371
(703 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g70310.1 68414.m08089 spermidine synthase 2 (SPDSYN2) / putre... 111 4e-25
At1g23820.2 68414.m03004 spermidine synthase 1 (SPDSYN1) / putre... 110 8e-25
At1g23820.1 68414.m03005 spermidine synthase 1 (SPDSYN1) / putre... 110 8e-25
At5g53120.3 68418.m06603 spermidine synthase, putative / putresc... 105 2e-23
At5g53120.2 68418.m06602 spermidine synthase, putative / putresc... 105 2e-23
At5g53120.1 68418.m06601 spermidine synthase, putative / putresc... 105 2e-23
At5g19530.1 68418.m02326 spermine/spermidine synthase family pro... 77 2e-14
At2g04920.1 68415.m00513 F-box family protein (FBX9) identical t... 31 0.74
At4g14580.1 68417.m02244 CBL-interacting protein kinase 4 (CIPK4... 30 1.3
At4g11800.1 68417.m01879 calcineurin-like phosphoesterase family... 29 3.0
At3g04350.1 68416.m00460 expressed protein 29 3.0
At5g11300.1 68418.m01319 cyclin, putative (CYC3b) similar to cyc... 28 5.2
At1g29900.1 68414.m03654 carbamoyl-phosphate synthase family pro... 28 6.9
At1g14460.1 68414.m01715 DNA polymerase-related weak similarity ... 28 6.9
>At1g70310.1 68414.m08089 spermidine synthase 2 (SPDSYN2) /
putrescine aminopropyltransferase 2 identical to
SP|O48661 Spermidine synthase 2 (EC 2.5.1.16)
(Putrescine aminopropyltransferase 2) (SPDSY 2)
{Arabidopsis thaliana}
Length = 340
Score = 111 bits (267), Expect = 4e-25
Identities = 48/93 (51%), Positives = 66/93 (70%)
Frame = +2
Query: 329 MDKLKTNWFTESCDMWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCT 508
M + WF+E MWPG S +V+++L KS YQ++ VF + + GKVLVLDG+IQ T
Sbjct: 44 MSSIIPGWFSEISPMWPGEAHSLKVEKILFQGKSDYQDVIVFQSATYGKVLVLDGVIQLT 103
Query: 509 QKDEFSYQEMISFLPLCCHKNPENVLIVGGGDG 607
++DE +YQEMI+ LPLC NP+ VL++GGGDG
Sbjct: 104 ERDECAYQEMITHLPLCSISNPKKVLVIGGGDG 136
Score = 30.7 bits (66), Expect = 0.98
Identities = 12/27 (44%), Positives = 20/27 (74%)
Frame = +1
Query: 622 EVAKHPQVKHIVLVEIDDRVIELSKRY 702
EVA+H V+ I + EID V++++K+Y
Sbjct: 141 EVARHSSVEQIDICEIDKMVVDVAKQY 167
>At1g23820.2 68414.m03004 spermidine synthase 1 (SPDSYN1) /
putrescine aminopropyltransferase 1 identical to
SP|Q9ZUB3 Spermidine synthase 1 (EC 2.5.1.16)
(Putrescine aminopropyltransferase 1) (SPDSY 1)
{Arabidopsis thaliana}
Length = 283
Score = 110 bits (265), Expect = 8e-25
Identities = 48/86 (55%), Positives = 64/86 (74%)
Frame = +2
Query: 350 WFTESCDMWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTQKDEFSY 529
WF+E MWPG S +V++VL KS YQ++ VF + + GKVLVLDG+IQ T++DE +Y
Sbjct: 47 WFSEMSPMWPGEAHSLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAY 106
Query: 530 QEMISFLPLCCHKNPENVLIVGGGDG 607
QEMI+ LPLC NP+ VL++GGGDG
Sbjct: 107 QEMITHLPLCSIPNPKKVLVIGGGDG 132
Score = 29.9 bits (64), Expect = 1.7
Identities = 11/27 (40%), Positives = 20/27 (74%)
Frame = +1
Query: 622 EVAKHPQVKHIVLVEIDDRVIELSKRY 702
EVA+H ++ I + EID V+++SK++
Sbjct: 137 EVARHASIEQIDMCEIDKMVVDVSKQF 163
>At1g23820.1 68414.m03005 spermidine synthase 1 (SPDSYN1) /
putrescine aminopropyltransferase 1 identical to
SP|Q9ZUB3 Spermidine synthase 1 (EC 2.5.1.16)
(Putrescine aminopropyltransferase 1) (SPDSY 1)
{Arabidopsis thaliana}
Length = 334
Score = 110 bits (265), Expect = 8e-25
Identities = 48/86 (55%), Positives = 64/86 (74%)
Frame = +2
Query: 350 WFTESCDMWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTQKDEFSY 529
WF+E MWPG S +V++VL KS YQ++ VF + + GKVLVLDG+IQ T++DE +Y
Sbjct: 47 WFSEMSPMWPGEAHSLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAY 106
Query: 530 QEMISFLPLCCHKNPENVLIVGGGDG 607
QEMI+ LPLC NP+ VL++GGGDG
Sbjct: 107 QEMITHLPLCSIPNPKKVLVIGGGDG 132
Score = 29.9 bits (64), Expect = 1.7
Identities = 11/27 (40%), Positives = 20/27 (74%)
Frame = +1
Query: 622 EVAKHPQVKHIVLVEIDDRVIELSKRY 702
EVA+H ++ I + EID V+++SK++
Sbjct: 137 EVARHASIEQIDMCEIDKMVVDVSKQF 163
>At5g53120.3 68418.m06603 spermidine synthase, putative / putrescine
aminopropyltransferase, putative similar to SP|O82147
Spermidine synthase (EC 2.5.1.16) (Putrescine
aminopropyltransferase) (SPDSY) {Coffea arabica};
contains Pfam profile PF01564: Spermine/spermidine
synthase
Length = 359
Score = 105 bits (253), Expect = 2e-23
Identities = 46/79 (58%), Positives = 62/79 (78%)
Frame = +2
Query: 371 MWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTQKDEFSYQEMISFL 550
MWPG S +V++VL +KS +Q + VF++ + GKVLVLDGI+Q T+KDE +YQEMI+ L
Sbjct: 77 MWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQLTEKDECAYQEMIAHL 136
Query: 551 PLCCHKNPENVLIVGGGDG 607
PLC +P+NVL+VGGGDG
Sbjct: 137 PLCSISSPKNVLVVGGGDG 155
>At5g53120.2 68418.m06602 spermidine synthase, putative / putrescine
aminopropyltransferase, putative similar to SP|O82147
Spermidine synthase (EC 2.5.1.16) (Putrescine
aminopropyltransferase) (SPDSY) {Coffea arabica};
contains Pfam profile PF01564: Spermine/spermidine
synthase
Length = 359
Score = 105 bits (253), Expect = 2e-23
Identities = 46/79 (58%), Positives = 62/79 (78%)
Frame = +2
Query: 371 MWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTQKDEFSYQEMISFL 550
MWPG S +V++VL +KS +Q + VF++ + GKVLVLDGI+Q T+KDE +YQEMI+ L
Sbjct: 77 MWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQLTEKDECAYQEMIAHL 136
Query: 551 PLCCHKNPENVLIVGGGDG 607
PLC +P+NVL+VGGGDG
Sbjct: 137 PLCSISSPKNVLVVGGGDG 155
>At5g53120.1 68418.m06601 spermidine synthase, putative / putrescine
aminopropyltransferase, putative similar to SP|O82147
Spermidine synthase (EC 2.5.1.16) (Putrescine
aminopropyltransferase) (SPDSY) {Coffea arabica};
contains Pfam profile PF01564: Spermine/spermidine
synthase
Length = 359
Score = 105 bits (253), Expect = 2e-23
Identities = 46/79 (58%), Positives = 62/79 (78%)
Frame = +2
Query: 371 MWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTQKDEFSYQEMISFL 550
MWPG S +V++VL +KS +Q + VF++ + GKVLVLDGI+Q T+KDE +YQEMI+ L
Sbjct: 77 MWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQLTEKDECAYQEMIAHL 136
Query: 551 PLCCHKNPENVLIVGGGDG 607
PLC +P+NVL+VGGGDG
Sbjct: 137 PLCSISSPKNVLVVGGGDG 155
>At5g19530.1 68418.m02326 spermine/spermidine synthase family
protein similar to SP|P09158 Spermidine synthase (EC
2.5.1.16) (Putrescine aminopropyltransferase)
{Escherichia coli}; contains Pfam profile PF01564:
Spermine/spermidine synthase
Length = 339
Score = 76.6 bits (180), Expect = 2e-14
Identities = 37/87 (42%), Positives = 53/87 (60%)
Frame = +2
Query: 347 NWFTESCDMWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTQKDEFS 526
+W+ E+ D +SF + VLH S+YQ+I + DT GKVLV+DG +Q ++DEF
Sbjct: 34 HWYEETID--DDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDGKMQSAERDEFI 91
Query: 527 YQEMISFLPLCCHKNPENVLIVGGGDG 607
Y E + L H NP+ V I+GGG+G
Sbjct: 92 YHECLIHPALLFHPNPKTVFIMGGGEG 118
Score = 27.9 bits (59), Expect = 6.9
Identities = 8/27 (29%), Positives = 18/27 (66%)
Frame = +1
Query: 622 EVAKHPQVKHIVLVEIDDRVIELSKRY 702
E+ KH ++ +V+ +ID V++ +R+
Sbjct: 123 EILKHTTIEKVVMCDIDQEVVDFCRRF 149
>At2g04920.1 68415.m00513 F-box family protein (FBX9) identical to
F-box protein family, AtFBX9 (GI:20197985) [Arabidopsis
thaliana]; contains F-box domain PF:00646; contains
TIGRFAM TIGR01640 : F-box protein interaction domain
Length = 376
Score = 31.1 bits (67), Expect = 0.74
Identities = 17/42 (40%), Positives = 23/42 (54%)
Frame = +2
Query: 392 SFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTQKD 517
S E K VL + S Y + QV+ + KV DG++ CT KD
Sbjct: 75 SIEFKSVLSLKDSHYNSEQVY----IAKVFHCDGLLLCTTKD 112
>At4g14580.1 68417.m02244 CBL-interacting protein kinase 4 (CIPK4)
identical to CBL-interacting protein kinase 4
[Arabidopsis thaliana] gi|13249503|gb|AAG01367;
identical to cDNA calcineurin B-like (CBL) interacting
protein kinase 4 (CIPK4) GI:13249502
Length = 426
Score = 30.3 bits (65), Expect = 1.3
Identities = 18/67 (26%), Positives = 29/67 (43%)
Frame = +2
Query: 344 TNWFTESCDMWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTQKDEF 523
T WF +S ++ + FE+ L E I FD SL L L G+ + ++ E
Sbjct: 274 TVWFQKSLEISEFQSSVFELDRFLEKEAKSSNAITAFDLISLSSGLDLSGLFERRKRKEK 333
Query: 524 SYQEMIS 544
+ +S
Sbjct: 334 RFTARVS 340
>At4g11800.1 68417.m01879 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 calcineurin-like
phosphoesterase
Length = 1012
Score = 29.1 bits (62), Expect = 3.0
Identities = 17/68 (25%), Positives = 29/68 (42%)
Frame = +2
Query: 350 WFTESCDMWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTQKDEFSY 529
WF D GG S+ V ++L + F + G VL++ G + F+Y
Sbjct: 369 WFDFMADTGDGGNSSYSVAKLLAQPSLRVPVANNFISLPRGNVLLIGGDLAYPNPSSFTY 428
Query: 530 QEMISFLP 553
++ + F P
Sbjct: 429 EKRL-FCP 435
>At3g04350.1 68416.m00460 expressed protein
Length = 567
Score = 29.1 bits (62), Expect = 3.0
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = -1
Query: 514 FLSALYDSVQDKNLPQTGCIKNLD 443
F YD ++ +P GC+KNLD
Sbjct: 227 FFCCTYDLSSERTVPDIGCLKNLD 250
>At5g11300.1 68418.m01319 cyclin, putative (CYC3b) similar to cyclin
3a [Arabidopsis thaliana] GI:509425; contains Pfam
profiles PF00134: Cyclin, N-terminal domain, PF02984:
Cyclin, C-terminal domain; identical to cDNA cyc3b mRNA
for cyclin 3b protein GI:728520
Length = 436
Score = 28.3 bits (60), Expect = 5.2
Identities = 28/112 (25%), Positives = 48/112 (42%), Gaps = 4/112 (3%)
Frame = +1
Query: 55 PQKIYLSTN---RSLSRVSVCHMYRIISLDIYIVIVKCEFSNVEFVRRAKRVFIFRARNT 225
P +YL+ N R LS S R+ L + +++ ++ E F F NT
Sbjct: 224 PDTLYLTVNLIDRFLSN-SYIERQRLQLLGVSCMLIASKYE--ELSAPGVEEFCFITANT 280
Query: 226 LFEREIVKTET*CQNLFNFRLQLPTWY*LLKQ-EKDGQTKNQLVYGIMRYVA 378
E++ E N +FRL +PT L++ K Q ++ + + Y+A
Sbjct: 281 YTRPEVLSMEIQILNFVHFRLSVPTTKTFLRRFIKAAQASYKVPFIELEYLA 332
>At1g29900.1 68414.m03654 carbamoyl-phosphate synthase family
protein similar to carbamoylphosphate synthetase
GI:6552726 from [Medicago sativa]; contains Pfam
profiles PF02786: Carbamoyl-phosphate synthase L chain
ATP binding domain, PF00289: Carbamoyl-phosphate
synthase L chain N-terminal domain, PF02787:
Carbamoyl-phosphate synthetase large chain
oligomerisation domain
Length = 1187
Score = 27.9 bits (59), Expect = 6.9
Identities = 22/79 (27%), Positives = 33/79 (41%), Gaps = 3/79 (3%)
Frame = +2
Query: 395 FEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTQKDEFSYQEMISFLPLCCHKNP 574
F K++ K+ + ++ SLG V + C + E M S + C P
Sbjct: 588 FSDKQIAFATKTTEEEVRT-KRISLGVVPSYKRVDTCAAEFEAHTPYMYSSYDVECESAP 646
Query: 575 EN---VLIVGGGDGRCRKG 622
N VLI+GGG R +G
Sbjct: 647 NNKKKVLILGGGPNRIGQG 665
>At1g14460.1 68414.m01715 DNA polymerase-related weak similarity to
DNA polymerase III holoenzyme tau subunit [Thermus
thermophilus] GI:2583049
Length = 1116
Score = 27.9 bits (59), Expect = 6.9
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = -3
Query: 251 VFTISRSNNVFLARNIKTRFALLTNSTLLNSHFT 150
V +I+ S + L RN++ R LL+ + LLNS T
Sbjct: 925 VSSITNSIEMVLRRNVEVRIILLSETELLNSKQT 958
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,574,631
Number of Sequences: 28952
Number of extensions: 337774
Number of successful extensions: 863
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 828
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 863
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1506636208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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