Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-0369
(683 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.09c |paa1||protein phosphatase regulatory subunit Paa1|S... 137 1e-33
SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizos... 44 2e-05
SPAC2F3.06c |kap104||karyopherin Kap104|Schizosaccharomyces pomb... 29 0.83
SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom... 28 1.4
SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosacc... 27 3.3
SPAC31G5.13 |rpn11|pad1, sks1, bfr2, mts5|19S proteasome regulat... 26 5.8
SPCC736.12c |||conserved protein|Schizosaccharomyces pombe|chr 3... 26 5.8
SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces pombe... 26 5.8
>SPAP8A3.09c |paa1||protein phosphatase regulatory subunit
Paa1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 590
Score = 137 bits (332), Expect = 1e-33
Identities = 70/151 (46%), Positives = 101/151 (66%)
Frame = +1
Query: 37 DMEQHVMPTVRARAGDTSWRVRYMVADKFVELQQAVGPELARTDLAQIFQALLKDSEAEV 216
++ +++ +R+ D+SWR RYMVA FV+L + VGP L + +L + F L+KD+E EV
Sbjct: 241 EIRHYLLQPLRSFVSDSSWRTRYMVAANFVKLAKVVGPSLIKDELIKPFVLLMKDTEQEV 300
Query: 217 RAAAAGKVKDFCMNLDKAHQEHIIMTMILPQIKDLVCDANQHVKSALASVIMGLSPIVGR 396
R A A ++ FC LDK I++ I+P I++L+ D QHV++AL I L+P +G+
Sbjct: 301 RRAIATQIPGFCELLDK----RIVLEEIIPVIQELINDPAQHVRAALGMNIGALAPQLGK 356
Query: 397 QNTIEHLLPLFLTQLKDECPEVRLNIISNLE 489
+ T E+LLP+FL LKDE PEVRLNIIS LE
Sbjct: 357 EKTTEYLLPMFLELLKDENPEVRLNIISKLE 387
Score = 44.8 bits (101), Expect = 1e-05
Identities = 33/132 (25%), Positives = 57/132 (43%)
Frame = +1
Query: 82 DTSWRVRYMVADKFVELQQAVGPELARTDLAQIFQALLKDSEAEVRAAAAGKVKDFCMNL 261
DT VR +A + + + + ++ + Q L+ D VRAA + L
Sbjct: 295 DTEQEVRRAIATQIPGFCELLDKRIVLEEIIPVIQELINDPAQHVRAALGMNIGALAPQL 354
Query: 262 DKAHQEHIIMTMILPQIKDLVCDANQHVKSALASVIMGLSPIVGRQNTIEHLLPLFLTQL 441
K ++ M L +KD N V+ + S + ++ +VG + + LLP +T
Sbjct: 355 GKEKTTEYLLPMFLELLKD----ENPEVRLNIISKLEVVNKVVGIELLSQSLLPAIVTLA 410
Query: 442 KDECPEVRLNII 477
+D+ VRL II
Sbjct: 411 EDKQWRVRLAII 422
Score = 44.8 bits (101), Expect = 1e-05
Identities = 32/154 (20%), Positives = 59/154 (38%)
Frame = +1
Query: 28 GTRDMEQHVMPTVRARAGDTSWRVRYMVADKFVELQQAVGPELARTDLAQIFQALLKDSE 207
G + Q ++P + A D WRVR + D L Q +G E + + + L+D
Sbjct: 394 GIELLSQSLLPAIVTLAEDKQWRVRLAIIDYIPLLAQQLGVEFFNEKMGNLCMSWLEDHV 453
Query: 208 AEVRAAAAGKVKDFCMNLDKAHQEHIIMTMILPQIKDLVCDANQHVKSALASVIMGLSPI 387
+R AA ++ L + I+P+ + N + I ++P
Sbjct: 454 YSIREAAIKNLR----KLTEIFGLEWATETIIPKFLAMRSHPNYLYRMTTIFAISEIAPA 509
Query: 388 VGRQNTIEHLLPLFLTQLKDECPEVRLNIISNLE 489
+ + + +LP + D P +R N+ E
Sbjct: 510 LNAEVIEKQILPTLEQLVNDPIPNIRFNVAKAFE 543
Score = 31.1 bits (67), Expect = 0.16
Identities = 31/137 (22%), Positives = 58/137 (42%)
Frame = +1
Query: 82 DTSWRVRYMVADKFVELQQAVGPELARTDLAQIFQALLKDSEAEVRAAAAGKVKDFCMNL 261
D + VR A + V + A + +F +L D + VR + + L
Sbjct: 177 DEAPMVRRPAATNCAKFVFLVTKQEAIDEFIPLFNSLSNDDQDSVRLLSFDIMVSLAEVL 236
Query: 262 DKAHQEHIIMTMILPQIKDLVCDANQHVKSALASVIMGLSPIVGRQNTIEHLLPLFLTQL 441
K+ E I +L ++ V D++ + +A+ + L+ +VG + L+ F+ +
Sbjct: 237 -KSDSE--IRHYLLQPLRSFVSDSSWRTRYMVAANFVKLAKVVGPSLIKDELIKPFVLLM 293
Query: 442 KDECPEVRLNIISNLEG 492
KD EVR I + + G
Sbjct: 294 KDTEQEVRRAIATQIPG 310
>SPBC119.07 |ppk19||serine/threonine protein kinase
Ppk19|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1706
Score = 44.0 bits (99), Expect = 2e-05
Identities = 27/101 (26%), Positives = 49/101 (48%)
Frame = +1
Query: 190 LLKDSEAEVRAAAAGKVKDFCMNLDKAHQEHIIMTMILPQIKDLVCDANQHVKSALASVI 369
LL DS + VR + + C+ KA +I++ ++ + D + ++ A I
Sbjct: 600 LLADSSSIVRRSLLNALAPLCVFFGKAKSNDLILSHLITYLND----TDWMLRCAFFESI 655
Query: 370 MGLSPIVGRQNTIEHLLPLFLTQLKDECPEVRLNIISNLEG 492
GLS +G ++ E++LPL L L D P V +++ + G
Sbjct: 656 TGLSIFIGPRSVDEYILPLMLQALVDPEPAVLESVLGSFSG 696
Score = 29.1 bits (62), Expect = 0.63
Identities = 23/96 (23%), Positives = 38/96 (39%)
Frame = +1
Query: 82 DTSWRVRYMVADKFVELQQAVGPELARTDLAQIFQALLKDSEAEVRAAAAGKVKDFCMNL 261
DT W +R + L +GP + + L D E V + G F +
Sbjct: 642 DTDWMLRCAFFESITGLSIFIGPRSVDEYILPLMLQALVDPEPAVLESVLG---SFSGLI 698
Query: 262 DKAHQEHIIMTMILPQIKDLVCDANQHVKSALASVI 369
+ E +++ IL + LV N +++ A SVI
Sbjct: 699 ELHLFEKLVVVDILQLVLPLVAVPNAYIRRAALSVI 734
Score = 25.8 bits (54), Expect = 5.8
Identities = 14/62 (22%), Positives = 34/62 (54%), Gaps = 1/62 (1%)
Frame = +1
Query: 295 MILPQIKDLVCDAN-QHVKSALASVIMGLSPIVGRQNTIEHLLPLFLTQLKDECPEVRLN 471
++LP + + N + K S++ LS + ++ ++ +LP +T L+D+ +VR++
Sbjct: 431 VLLPIVLSTIRHVNTRESKINALSLVQILSRNICDESKLDTVLPFVMTLLRDQYADVRIS 490
Query: 472 II 477
+
Sbjct: 491 AL 492
>SPAC2F3.06c |kap104||karyopherin Kap104|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 910
Score = 28.7 bits (61), Expect = 0.83
Identities = 23/68 (33%), Positives = 28/68 (41%), Gaps = 2/68 (2%)
Frame = +1
Query: 58 PTVRARAGDTSWRVRYMVADKFVELQQAVGPELARTD--LAQIFQALLKDSEAEVRAAAA 231
PTV D +V+ L V P LA+ D L QI KD EVR +A
Sbjct: 651 PTVDVPDRDFLVTTLDLVSGIIQALGSQVSPLLAQADPPLGQIIGICAKDEVPEVRQSAY 710
Query: 232 GKVKDFCM 255
+ D CM
Sbjct: 711 ALLGDMCM 718
>SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1067
Score = 27.9 bits (59), Expect = 1.4
Identities = 27/96 (28%), Positives = 50/96 (52%), Gaps = 4/96 (4%)
Frame = +1
Query: 166 DLAQIFQALLKDSEAEVRAAAAGKVKDFC-MNLDKAHQEHIIMTMILPQIKDLVCDANQH 342
D+ +F A L+DSE EVR+ AA + C + + E++ IL +++ +
Sbjct: 894 DVFSLFMAALEDSEGEVRSNAAYSMGLLCQFSTEDLSSEYL---NILQKLQPFF--TQEV 948
Query: 343 VKSALASVIMGLSP-IVGRQNTI--EHLLPLFLTQL 441
++AL + I +S I+ QN I + +LP+ ++L
Sbjct: 949 FRTALDNAIGCISRLILHNQNAIPVDQVLPIVFSKL 984
>SPCC132.04c |||NAD-dependent glutamate dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1106
Score = 26.6 bits (56), Expect = 3.3
Identities = 9/42 (21%), Positives = 20/42 (47%)
Frame = -1
Query: 419 RRCSIVFCLPTMGLRPMMTEARADLTCWLASHTKSLICGKIM 294
R S+++CLP+ +P+ + + +H + C +M
Sbjct: 387 REASLLYCLPSTDFQPLFVSEKLSIQEVTYAHCVRIFCEHVM 428
>SPAC31G5.13 |rpn11|pad1, sks1, bfr2, mts5|19S proteasome regulatory
subunit Rpn11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 308
Score = 25.8 bits (54), Expect = 5.8
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = +3
Query: 606 AHPPFASWRNSEEARTDRPFQQL 674
+HP F W +S + T + F+QL
Sbjct: 113 SHPGFGCWLSSVDINTQQSFEQL 135
>SPCC736.12c |||conserved protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 437
Score = 25.8 bits (54), Expect = 5.8
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = +3
Query: 450 MSRSPTQHNLQPRGGARYPIRPIVSRITIHWPS 548
+S T+H + G RYP P +R + H PS
Sbjct: 234 LSHEDTRHGIASSGSTRYPFVPANTRAS-HSPS 265
>SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 570
Score = 25.8 bits (54), Expect = 5.8
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = -3
Query: 321 QVLDLWQDHGHDDVFLMSFIQVHTEVLDLP 232
++LDL++D ++ ++VH VLD P
Sbjct: 525 ELLDLFKDEEFGKADVIQLVEVHMPVLDAP 554
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,809,260
Number of Sequences: 5004
Number of extensions: 54836
Number of successful extensions: 178
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 166
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 315915086
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -